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| 1 | Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice显示文摘AIM: To study whether matrix metalloproteinase-9 (MMP-9) is a key factor in epithelial damage in the dextran sodium sulphate (DSS) model of colitis in mice.METHODS: MMP-9-deficient and wild-type (wt) mice were given 5% DSS in drinking water for 5 d followed by recovery up to 7 d. On d 5 and 12 after induction of colitis, gelatinases, MMP-2 and MMP-9, were measured in homogenates of colonic tissue by zymography and Western blot, whereas tissue inhibitor of metalloproteinases (TIMPs) were measured by reverse zymography. The gelatinolytic activity was also determined in supernatants of polymorphonuclear leukocytes (PMN) isolated from mice blood. Moreover, intestinal epithelial cells were stimulated with TNF-α to study whether these cells were able to produce MMPs. Finally, colonic mucosal lesions were measured by microscopic examination. RESULTS: On d 5 of colitis, the activity of MMP-9 was increased in homogenates of colonic tissues (0.24 ± 0.1 vs 21.3 ± 6.4, P < 0.05) and PMN from peripheral blood in wt (0.5 ± 0.1 vs 10.4 ± 0.7, P < 0.05), but not in MMP-9-deficient animals. The MMP-9 activity was also up-regulated by TNF-α in epithelial intestinal cells (2.5 ± 0.5 vs 14.7 ± 3.0, P < 0.05). Although colitis also led to increase of TIMP-1 activity, the MMP-9/TIMP-1 balance remained elevated. Finally, in the MMP-9-deficient colitic mice both the extent and severity of intestinal epithelialinjury were significantly attenuated when compared with wt mice. CONCLUSION: We conclude that DSS induced colitis is markedly attenuated in animals lacking MMP-9. This suggests that intestinal injury induced by DSS is modu-lated by MMP-9 and that inhibition of this gelatinase may reduce inflammation. | Alfredo Santana Carlos Medina Maria Cristina Paz-Cabrera Federico Díaz-Gonzalez Esther Farré Antonio Salas Marek W Radomski Enrique Quintero | 2006 | World Journal of Gastroenterology2006，12，40： | 9 |
| 2 | The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection 显示文摘 | Di Cristina M Del Porto P Buffolano W | 2004 | Microbes Infect2004，6，2： | 1 |
| 3 | In vivo and in vitro inhibition of mice thioredoxin reductase by methylmercury 显示文摘 | WAGNER C SUDATI J H CRISTINA W | 2010 | Biometals2010，23，6： | 1 |
| 4 | Organoselenium and organotellurirm compounds:toxicology and pharmacology显示文摘 | CRISTINA W N GILSON Z JOAO B T | 2004 | Chem Rev2004，104，： | 1 |
| 5 | Acute liver damage induced by 2-nitropropane in rats: Effect of diphenyl diselenide on antioxidant defenses 显示文摘 | LYSANDRO P CRISTINA W | 2006 | Chemico-Biological Interactions2006，160，2： | 1 |
| 6 | Nitroglycerin induces hyperalgesia in rats a time course study显示文摘 | Cristina T Rosaria G Dechun W | 2003 | Eur J Pharmacol2003，464，23： | 1 |
| 7 | Chronic care management for the eld- erly : an opportunity for gerontological nurse practitioners 显示文摘 | Cristina C Hendrik Colleen W | 2005 | Journal of the American Academy of nurse practitioners2005，17，7： | 1 |
| 8 | Intracellular lipid accumulation in liver and muscle and the insulin resistance syndrome显示文摘 | Cristina LC Timothy W | 2008 | Endocrinol Metab Clin North Am2008，37，4： | 1 |
| 9 | Rapid analytical procedure for determination of mineral oils in edible oil by GC-FID显示文摘 | MAGDALENA W DAVINSON P Cristina Nerin | 2013 | Food Chemistry2013，141，4： | 1 |
| 10 | The effects of storing seeds under extreme dry conditions显示文摘 | Cristina W Jan E | 1998 | Seed Science Research 81998，，： | 1 |
| 11 | The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection 显示文摘 | Porto PD Buffolano W | 2004 | Microbes and Infection2004，6，： | 1 |
| 12 | A Novel Active Packaging for Extending the Shelf-life of Fresh Mushrooms (Agaricus Bisporus) 显示文摘 | MAGDALENA W KARIM B CRISTINA N | 2015 | Food Control2015，54，： | 1 |
| 13 | Effect of calcium dobesilate on occurrence of diabetic macular oedema (CALDIRET study): randomised, double-blind, placebo-controlled, multicentre trial显示文摘 | Christos Haritoglou Joachim Gerss Cristina Sauerland Anselm Kampik Michael W Ulbig | 2009 | The Lancet2009，，9672： | 1 |
| 14 | Acute liver damage induced by 2-nitro- propane in rats: Effect of diphenyl diselenide on antioxidant de- fenses显示文摘 | Lysandro P Cristina W | 2006 | Chemieo-Biologieal Interactions2006，160，2： | 1 |
| 15 | Relative effect of particle size on the physical properties of corn meal extmdates:Effect of particle size on the extrusion of corn meal显示文摘 | CARLOS W P CRISTINA Y T CHARLES I O | 2010 | Journal of Food Engineering2010，98，： | 1 |
| 16 | Nitroglycerin Induces Hyperalgesia in Rats a Time-course Study显示文摘 | CRISTINA T ROSARIA G DECHUN W | 2003 | Eur J Pharmacol(S0014 - 2999)2003，464，23： | 1 |
| 17 | 6-Methylapigenin and hesperidin : new valeriana flavonoids with activity on the CNS 显示文摘 | MARIEL M HAYDEE V CRISTINA W etal | 2003 | Pharmacology Biochemistry and Behavior2003，，75： | 1 |
| 18 | Dopamine receptors;from structure to function显示文摘 | Cristina Missale Russel Nash S Susan W | 1998 | The Americal Physiological Society1998，78，1： | 1 |
| 19 | Spinal-cord injury显示文摘 | John W McDonald Cristina Sadowsky | 2002 | The Lancet2002，，9304： | 1 |
| 20 | Rocha organoselenium and organotellurirm compounds显示文摘 | CRISTINA W N GILSON Z JOAO B T | 2004 | Toxicology Pharmacology2004，104，24： | 1 |
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