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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | Influence of heart rate on vessel visibility in noninvasive coronary angiography using new multislice computed tomography显示文摘 | Stephen Schroeder Andreas F Kopp Axel Kuettner Christof Burgstahler Christian Herdeg Martin Heuschmid Andreas Baumbach Claus D Claussen Karl R Karsch Ludger Seipel | 2002 | Clinical Imaging2002，，2： | 2 |
| 3 | Stable RNA interference rules for silencing显示文摘 | Christof F Scott W L | 2014 | Nat Cell Biol2014，16，1： | 1 |
| 4 | Technical advances in digital audio radio broadcasting显示文摘 | CHRISTOF F BIING-HWANG J | 2002 | Proceeding of the IEEE2002，90，8： | 1 |
| 5 | Erythropoietin mRNA expression in human fetal and neonatal tissue 显示文摘 | Christof D Hubert F Patricia F | 1998 | Blood1998，92，： | 1 |
| 6 | Local and remote visualization techniques for interactive direct volume rendering in neuroradiology显示文摘 | Bernd F T Peter H Christof R S | 2001 | RadioGraphics2001，21，6： | 1 |
| 7 | Influence of heart rate on vessel visibility in noninvasive coronary angiography using new multislice computed tomography显示文摘 | Stephen Schroeder Andreas F Kopp Axel Kuettner Christof Burgstahler Christian Herdeg Martin Heuschmid Andreas Baumbach Claus D Claussen Karl R Karsch Ludger Seipel | 2002 | Clinical Imaging2002，，2： | 1 |
| 8 | Economic development and the demand for energy: A historical perspective on the next 20 years 显示文摘 | Christof R Paul A Julian F | 2012 | Energy Policy2012，50，： | 1 |
| 9 | Local and remote visualization techniques for interactive direct volume rendering in neuroradiology 显示文摘 | BERND F T PETER H CHRISTOF R S | 2001 | RadioGraphics2001，21，6： | 1 |
| 10 | Inhibition of tumor necrosis factor- a improves postischemic recovery of hypertrophied hearts 显示文摘 | Christof S lngcborg F Douglas B | 2001 | Circulation2001，104，： | 1 |
| 11 | Erythropoietin mRNA expression in human fetal and neonatal tissue 显示文摘 | Christof D Hubert F Patricia F | 1998 | Blood1998，92，： | 1 |
| 12 | A distributed robotic control system based on a temporal self- organizing neural network 显示文摘 | GUILHERME A Barreto ALUIZIO F R Araujo CHRISTOF Ducker HELGE Ritter | 2002 | IEEE Transactions on System Man and Cybernetics-Part C: Applications and Reviews2002，32，4： | 1 |
| 13 | Improved total variation - based CT image reconstruction applied to clinical data 显示文摘 | Ludwig H Frank B Christof F | 2011 | Physics in Medicine & Biology2011，56，6： | 1 |
| 14 | Insecticidal Activity and Mode of Action of Novel Nicotinoids Synthesized by New Acylpyridinium Salt Chemistry and Directed Lithiation 显示文摘 | CHRISTOF F S SPARKS T C DONOHUE K V | 2007 | Pesticide Biochemistry and Physiology2007，87，： | 1 |
| 15 | A direct quantitative relationship between the functional properties of human and macaque V5 显示文摘 | Karl F Christof K | 2000 | Nature Neuroscience2000，3，7： | 1 |
| 16 | SAP HANA database显示文摘 | Franz F?rber Sang Kyun Cha Jürgen Primsch Christof Bornh?vd Stefan Sigg Wolfgang Lehner | 2012 | ACM SIGMOD Record2012，，4： | 1 |
| 17 | Monitoring spatiotemporal soil moisture changes in the subsurface of forest sites using electrical resistivity tomography (ERT)显示文摘The effects of drought on tree mortality at forest stands are not completely understood. For assessing their water supply, knowledge of the small-scale distribution of soil moisture as well as its temporal changes is a key issue in an era of climate change. However, traditional methods like taking soil samples or installing data loggers solely collect parameters of a single point or of a small soil volume. Electrical resistivity tomography (ERT) is a suitable method for monitoring soil moisture changes and has rarely been used in forests. This method was applied at two forest sites in Bavaria, Germany to obtain high-resolution data of temporal soil moisture variations. Geoelectrical measurements (2D and 3D) were conducted at both sites over several years (2015-2018/2020) and compared with soil moisture data (matric potential or volumetric water content) for the monitoring plots. The greatest variations in resistivity values that highly correlate with soil moisture data were found in the main rooting zone. Using the ERT data, temporal trends could be tracked in several dimensions, such as the interannual increase in the depth of influence from drought events and their duration, as well as rising resistivity values going along with decreasing soil moisture. The results reveal that resistivity changes are a good proxy for seasonal and interannual soil moisture variations. Therefore, 2D- and 3D-ERT are recommended as comparatively non-laborious methods for small-spatial scale monitoring of soil moisture changes in the main rooting zone and the underlying subsurface of forested sites. Higher spatial and temporal resolution allows a better understanding of the water supply for trees, especially in times of drought. | Julian Fäth Julius Kunz Christof Kneisel | 2022 | Journal of Forestry Research2022，33，5： | 0 |
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