|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Molecular epidemiological analysis of Escherichia coli isolates producing Extend-spectrumβ-laetamasefou identification of Nosocomial ourbreaks in Stockholm, Sweden显示文摘 | Hong F Christina L Barbro OL | 2004 | J Clin Microbiol2004，42，12： | 1 |
| 2 | Milk plasmin activity influence oncheddar cheese quality during ripening显示文摘 | NANA Y F CHRISTINA F L | 1992 | Journal of Food Science1992，57，3： | 1 |
| 3 | Thin colagen film scaffolds for retinalepithelialcel culture显示文摘 | James T L Christina J L Stacey F B | | 0，，08： | 1 |
| 4 | Controls on the PGE distribution of Permian Emeishan alkaline and peralkaline volcanic rocks in Longzhoushan,Sichuan Province,SW China显示文摘 | Qi L Christina W Y Zhou M F | 2008 | Lithos2008，106，： | 1 |
| 5 | Compounding and molding of polyethylene composites reinforced with keratin feather fiber显示文摘 | Justin R B Walter F S Christina F E L | 2005 | Composites Science and Technology2005，65，： | 1 |
| 6 | Molecular epidemiological analysis of Escherichia coli isolates producing Extend- spectrumβ-1actamasefou identification of Nosocomial ourbreaks in Stockholm, Sweden 显示文摘 | Hong F Christina L Barbro OL | 2004 | J Clin Microbiol2004，42，12： | 1 |
| 7 | Compounding and molding of polyethylene composites reinforced with kera- tin feather fiber 显示文摘 | Justin R B Walter F St Christina F E L | 2005 | Composites Science and Technology2005，65，34： | 1 |
| 8 | Intrinsic motivation and exercise adherence显示文摘 | RICHARD M CHRISTINA M F DEBORAH L S | 1997 | 1NT J Sport Psychol1997，28，4： | 1 |
| 9 | Polyurethane surfaces modified by amphiphillc Polymers:effects on protein adsorption显示文摘 | Patric J Bengt W | 2000 | Biomaterials2000，21，： | 1 |
| 10 | Family - centered care: Helping parents to help their child with procedural and everyday pain:Practical, evidence - based advice显示文摘 | Nina P Christina L Linda F | 2007 | JSPN2007，12，3： | 1 |
| 11 | Efficient broadcasting usingnetwork coding显示文摘 | CHRISTINA F L JORG W | 2008 | IEEE Transactions on Networking2008，16，2： | 1 |
| 12 | Systematic significance of cell inclusions in Haemodoraceae and allied famillies:silica bodies and tapetal raphides显示文摘 | CHRISTINA J P CAROL A F PAULA J R L | 2003 | Annals of Botany2003，92，： | 1 |
| 13 | 显示文摘 | Christina F L Patric J Bengt W | 2000 | Biomaterials2000，21，： | 1 |
| 14 | Stakeholder collaboration and heritage management显示文摘 | Christina A Adele L John F | 2005 | Annals of Tourism Research2005，32，1： | 1 |
| 15 | Trends in predicting and controlling ash vaporization in coal-fired utility boilers显示文摘 | ERIC G E ADEL F S CHRISTINA M L | 2001 | Fuel Processing Technology2001，71，： | 1 |
| 16 | Thin collagen film scaffolds for retinal epithelial cell culture显示文摘 | James T L Christina J L Stacey F B | 2007 | Biomaterials2007，28，8： | 1 |
| 17 | Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and-susceptible chickens显示文摘 | CHRISTINA L S MICHAEL H K PAMELA J F | 2004 | Immunology2004，113，1： | 1 |
| 18 | Final report of the amended safety assessment of pvm/ma copolymer and its related salts and esters as used in cosmetics 显示文摘 | Christina L Burnett Wilma F Belsito DV | 2011 | International Journal of Toxicology2011，30，： | 1 |
| 19 | Apolipoprotein B100 is required for hepatitis C infectivity and Mipomersen inhibits hepatitis C显示文摘AIM To characterize the role of apolipoprotein B100(apoB 100) in hepatitis C viral(HCV) infection. METHODS In this study, we utilize a gene editing tool, transcription activator-like effector nucleases(TALENs), to generate human hepatoma cells with a stable genetic deletion of APOB to assess of apoB in HCV. Using infectious cell culture-competent HCV, viral pseudoparticles, replicon models, and lipidomic analysis we determined the contribution of apoB to each step of the viral lifecycle. We further studied the effect of mipomersen, an FDAapproved antisense inhibitor of apoB 100, on HCV using in vitro cell-culture competent HCV and determined itsimpact on viral infectivity with the TCID50 method. RESULTS We found that apo B100 is indispensable for HCV infection. Using the JFH-1 fully infectious cell-culture competent virus in Huh 7 hepatoma cells with TALENmediated gene deletion of apoB(APOB KO), we found a significant reduction in HCV RNA and protein levels following infection. Pseudoparticle and replicon models demonstrated that apo B did not play a role in HCV entry or replication. However, the virus produced by APOB KO cells had significantly diminished infectivity as measured by the TCID-50 method compared to wildtype virus. Lipidomic analysis demonstrated that these virions have a fundamentally altered lipidome, with complete depletion of cholesterol esters. We further demonstrate that inhibition of apoB using mipomersen, an FDA-approved anti-sense oligonucleotide, results in a potent anti-HCV effect and significantly reduces the infectivity of the virus. CONCLUSION Apo B is required for the generation of fully infectious HCV virions, and inhibition of apo B with mipomersen blocks HCV. Targeting lipid metabolic pathways to impair viral infectivity represents a novel host targeted strategy to inhibit HCV. | Esperance AK Schaefer James Meixiong Christina Mark Amy Deik Daniel L Motola Dahlene Fusco Andrew Yang Cynthia Brisac Shadi Salloum Wenyu Lin Clary B Clish Lee F Peng Raymond T Chung | 2016 | World Journal of Gastroenterology2016，22，45： | 1 |
| 20 | German population data of three tetrameric short tandem repeat lociD3S1744, D12S1090 and D18S849 显示文摘 | Christina S Thomas L Stanislav F | 1998 | Forensic Science International1998，91，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员