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| 1 | 在诊断 choledochal 包囊的磁性的回声 cholangiopancreatography 的角色: 盒子系列和评论显示文摘 AIM:To determine the merits of magnetic resonance cholangiopancreatography(MRCP) as the primary diagnostic test for choledochal cysts(CC’s).METHODS:Between 2009 and 2012,patients who underwent MRCP for perioperative diagnosis were identified.Demographic information,clinical characteristics,and radiographic findings were recorded.MRCP results were compared with intraoperative findings.A PubMed search identified studies published between 1996-2012,employing MRCP as the primary preoperative imaging and comparing results with either endoscopic retrograde cholangiopancreatography(ERCP) or operative findings.Detection rates for CC’s and abnormal pancreaticobiliary junction(APBJ) were calculated.In addition detection rates for clinically related biliary pathology like choledocholithiasis and cholangiocarcinomas in patients diagnosed with CC’s were also evaluated.RESULTS:Eight patients were identified with CC’s.Six patients out of them had typeⅣCC’s,1 had type I and 1 had a new variant of choledochal cyst with confluent dilatation of the common bile duct(CBD) and cystic duct.Seven patients had an APBJ and 3 of those had a long common-channel.Gallstones were found in 2 patients,1 had a CBD stone,and 1 pancreatic-duct stone was also detected.In all cases,MRCP successfully identified the type of CC’s,as well as APBJ with ductal stones.From analyzing the literature,we found that MRCP has 96%-100% detection rate for CC’s.Additionally,we found that the range for sensitivity,specificity,and diagnostic accuracy was 53%-100%,90%-100% and 56%-100% in diagnosing APBJ.MRCP’s detection rate was 100% for choledocholithiasis and 87% for cholangiocarcinomas with concurrent CC’s.CONCLUSION:After initial ultrasound and computed tomography scan,MRCP should be the next diagnostic test in both adult and pediatric patients.ERCP should be reserved for patients where therapeutic intervention is needed. | Vikas Y Sacher James S Davis Danny Sleeman Javier Casillas | 2013 | World Journal of Radiology2013，5，8： | 15 |
| 2 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 3 | Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy显示文摘AIM To detect hyper-conserved regions in the hepatitis B virus(HBV) X gene(HBX) 5' region that could be candidates for gene therapy.METHODS The study included 27 chronic hepatitis B treatmentnaive patients in various clinical stages(from chronic infection to cirrhosis and hepatocellular carcinoma, both HBeA g-negative and HBeA g-positive), and infected with HBV genotypes A-F and H. In a serum sample from each patient with viremia > 3.5 log IU/m L, the HBX 5' end region [nucleotide(nt) 1255-1611] was PCRamplified and submitted to next-generation sequencing(NGS). We assessed genotype variants by phylogenetic analysis, and evaluated conservation of this region by calculating the information content of each nucleotide position in a multiple alignment of all unique sequences(haplotypes) obtained by NGS. Conservation at the HBx protein amino acid(aa) level was also analyzed.RESULTS NGS yielded 1333069 sequences from the 27 samples, with a median of 4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of viral nucleotide haplotypes showed a complex mixture of genotypic variants. Analysis of the information content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the HBX upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded for a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like domain.CONCLUSION Two hyper-conserved regions detected in the HBX 5' end may be of value for targeted gene therapy, regardless of the patients' clinical stage or HBV genotype. | Carolina González David Tabernero Maria Francesca Cortese Josep Gregori Rosario Casillas Mar Riveiro-Barciela Cristina Godoy Sara Sopena Ariadna Rando Marcal Yll Rosa Lopez-Martinez Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，19： | 6 |
| 4 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 5 | Assessing availability, phytotoxicity and bioaccumulation of lead to ryegrass and millet based on 0.1 mol/L Ca(NO_3)_2 extraction显示文摘This study was conducted to assess availability, phytotoxicity and bioaccumulation of lead (Pb) to ryegrass (Lolium perenne L.) and millet (Echinochloa crusgalli) based on the 0.1 mol/L Ca(NO3)2 extraction. Effect of soil properties on availability, phyto- toxicity and bioaccumulation of Pb to the two plants was also evaluated. Five soils with pH values varying from 3.8 to 7.3, organic carbon (OC) contents from 0.7% to 2.4%, and clay contents from 11.6% to 35.6% were selected. Soils were spiked with Pb to achieve a range of concentrations: 250, 500, 1000, 3000 and 5000 mg/kg. Pb availability in the spiked soils was estimated by extracting soil with 0.1 mol/L Ca(NO3)2. The results indicate that plants yield decreased with decreasing soil pH and increased with increasing soil clay and OC content. Negative relationship between available Pb and the relative dry matter growth (RDMG) of the two plants were significantly related. Available Pb used to assess EC20 (20% effective concentration) and EC50 (50% effective concentration) of millet was 119 and 300 mg/kg, respectively. Available Pb used to assess EC20 and EC50 of ryegrass was 63 and 157 mg/kg, respectively. Bioaccumulation, expressed as bioconcentration factors of Pb, was inversely related to soil pH, soil OC and clay content. Strong relationships were found between available lead and uptake by the two plants (r2 was 0.92 and 0.95 respectively). In general, 0.1 mol/L Ca(NO3)2 available Pb may be used to assess the availability, phytotoxicity and bioaccumulation of lead to the two plants tested. | SI Ji-tao TIAN Bao-guo WANG Hong-tao Nicholas Basta Jackie Schroder Mark Casillas | 2006 | Journal of Environmental Sciences2006，18，5： | 4 |
| 6 | Studies on the Effects of Deltamethrin on Sodium Net Transport Through the in vivo Amphibian Skin显示文摘The action of micromolar concentrations of Deltamethrin on sodium net transport through the in vivo skin of the South American toad Bufo arenarum was studied. The effect of pure ethanolic insecticide solutions and commercial formulations when applied on the mucosal surface was assayed. Deltamethrin provoked a concentration-independent inhibition; the highest inhibition was found at the lowest concentrations. At highest concentrations of the insecticide the J Na was not | ALFREDO SALIBIAN AND JOSE L. MARAZZO(Programa de Ecofisiologia Aplicada, Departamento de Ciencias Basicas,Universidad Nacional de Lujan, Casilla de Correo 221, 6700-Lujaan (B),Argentina)(Comision de Investigaciones Cientificas, Provincia de Buenos Aires, | 1995 | Biomedical and Environmental Sciences1995，8，2： | 3 |
| 7 | Biliary-colonic fistula caused by cholecystectomy bile duct injury显示文摘Biliary-colonic fistula is a rare complication after laparoscopic cholecystectomy. We present a case of post-cholecystectomy iatrogenic biliary injury that resulted in a fistula between the common hepatic duct and large bowel. Magnetic resonance cholangiopancreatography provided good visualization of injury even with concurrent normal level of alkaline phosphatase. Radiologic findings and surgical management of this condition are discussed in detail. | Francisco Igor B Macedo Victor J Casillas James S Davis Joe U Levi Danny Sleeman | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，4： | 2 |
| 8 | Betaglycan presents ligand to the TGF - βsignaling receptor显示文摘 | Casillas LF Cheife Z Doody J | 1993 | Cell1993，73，： | 2 |
| 9 | Total synthesis of O-methylsterigrnatocystin using N-alkylnitrilium salts and carbonyl-alkene interconversion in a new xanthone synthesis 显示文摘 | Casillas L K Townsend C A | 1999 | J Org Chem1999，64，11： | 1 |
| 10 | Early laparoscopic cholecystectomy is the preferred management of acute cholecystitis显示文摘 | Casillas RA Yegiyants S Collins JC | 2008 | Arch Surg2008，143，6： | 1 |
| 11 | Early laparoscopic cholecystectomy is the preferred management of acute cholecystitis 显示文摘 | Robert A Casillas MD Sara Y | 2008 | Arch Surg2008，143，6： | 1 |
| 12 | Early laparoscopic cholecystectomy is the preferred management of acute cholecystitis显示文摘 | Casillas RA Yegiyauts S Collins JC | | 0，，： | 1 |
| 13 | Highly selective fenuron-imprinted polymer with a homogeneous binding site distribution prepared by precipitation polymerisation and its application to the clean-up of fenuron in plant samples显示文摘 | Tamayo FG Casillas JL Martin-Esteban A | 2003 | Anal Chim Acta2003，482，2： | 1 |
| 14 | Relationship of serum chemistry values to liver and kidney histopathology in English sole (Parophrys vetulus) after acute exposure to carbon tetrachloride 显示文摘 | Casillas E Myers M Ames W E | 1983 | Aquatic Toxicol1983，3，： | 1 |
| 15 | Is Laparoscopic Colectomy Applicable to Patients With Body Mass Index >30? A Case-Matched Comparative Study With Open Colectomy显示文摘 | Conor P. Delaney M.Ch. Ph.D. F.R.C.S.I.(Gen.) Naveen Pokala M.B.B.S. M.S.(Gen.) F.R.C.S. Anthony J. Senagore M.D. M.S. M.B.A. Sergio Casillas M.D. Ravi P. Kiran M.B.B.S M.S.(Gen. Surg.) F.R.C.S. Karen M. Brady B.S.N. R.N. Victor W. Fazio M.B. M. | 2005 | Diseases of the Colon & Rectum2005，，5： | 1 |
| 16 | Pathophy of myasthenin gravis显示文摘 | Hughes BW Moro De Casillas ML Kaminski HJ | 2004 | Semin Neurol2004，24，： | 1 |
| 17 | Multicenter e-valuation of the efficacy and safety of gatifloxacin in Mexican a-dult out patients with respiratory tract infections显示文摘 | Casillas JL Rico G Rodriguez-Parga D | 2000 | Adv T-her2000，17，6： | 1 |
| 18 | Does conversion of a laparoscopic colectomy adversely affect patient outcome? 显示文摘 | Casillas S Delaney CP Senagore A J | 2004 | Dis Colon Rectum2004，47，10： | 1 |
| 19 | Histopathologic features seen with differentanimal models following cutaneous sulfur mustard exposure显示文摘 | Smith KJ Casillas R Graham J | 1997 | J Dermatol Sci1997，14，： | 1 |
| 20 | Perfusion to colorectal cancer liver metastases is not uniform and depends on tumor location and feeding vessel 显示文摘 | Casillas S Dietz DW Brand MI | 1997 | J Surg Res1997，67，2： | 1 |
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