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| 1 | Bifunctional chimeric SuperCD suicide gene -YCD: YUPRT fusion is highly effective in a rat hepatoma model显示文摘AIM: To investigate the effects of catalytically superior gene-directed enzyme prodrug therapy systems on a rat hepatoma model.METHODS: To increase hepatoma cell chemosensitivity for the prodrug 5-fluorocytosine (5-FC), we generated a chimeric bifunctional SuperCD suicide gene, a fusion of the yeast cytosine deaminase (YCD) and the yeast uracil phosphoribosyltransferase (YUPRT) gene.RESULTS: In vitro stably transduced Morris rat hepatoma cells (MH) expressing the bifunctional SuperCD suicide gene (MH SuperCD) showed a clearly marked enhancement in cell killing when incubated with 5-FC as compared with MH ceils stably expressing YCD solely (MH YCD) or the cytosine deaminase gene of bacterial origin(MH BCD), respectively. In vivo, MH SuperCD tumors implanted both subcutaneously as well as orthotopically into the livers of syngeneic ACI rats demonstrated significant tumor regressions (P<0.01) under both high dose as well as low dose systemic 5-FC application,whereas MH tumors without transgene expression (MH naive) showed rapid progression. For the first time, an order of in vivo suicide gene effectiveness (SuperCD>>YCD > > BCD > > > negative control) was defi ned as a result of a directin vivo comparison of all three suicide genes.CONCLUSION: Bifunctional SuperCD suicide gene expression is highly effective in a rat hepatoma model,thereby significantly improving both the therapeutic index and the efficacy of hepatocellular carcinoma killing by fluorocytosine. | Florian Graepler Marie-Luise Lemken Wolfgang A Wybranietz Ulrike Schmidt Irina Smirnow Christine D GroB Martin Spiegel Andrea Schenk Schenk Hansj(o|¨)rg Graf Ulrike A Lauer Reinhard Vonthein Michael Gregor Sorin Armeanu Michael Bitzer Ulrich M.Lauer | 2005 | World Journal of Gastroenterology2005，11，44： | 2 |
| 2 | Lunrbar facet joint effusion in MRI: a sign of instability in degenerative spondylolisthesis? 显示文摘 | Latting F Fekete TF Grob D | 2012 | Eur Spine J2012，21，2： | 1 |
| 3 | Translaminar screw fixation of the lumbar spine : long - term outcome 显示文摘 | Aepli M Mannion AF Grob D | 2009 | Spine ( Phila Pa 1976)2009，34，14： | 1 |
| 4 | Clinical experience with the Dynesys semirigid fixation system for the lumbar spine:surgical and patient-oriented outcome in 50 cases after an average of 2 years显示文摘 | Benini A Junge A | 2005 | J Spine2005，30，3： | 1 |
| 5 | A deletion in the bovine myostatin gene cause the double-muscled phenotype in cattle 显示文摘 | Grob I Martin L J Poncelet D | 1997 | Nat Genet1997，17，1： | 1 |
| 6 | Posterior surgical approaeh to the lumbar spine and its effect on the multifidus muscle 显示文摘 | Weber BR Grob D Dvorak J | 1997 | Spine1997，22，15： | 1 |
| 7 | Translaminar screw fixation in the lumbar spine:techniqe, indication ,results显示文摘 | Grob D humke T | 1998 | Eur Spine J1998，7，3： | 1 |
| 8 | Lifetime course ofmyasthenia gravis显示文摘 | Grob D Brunner N Namba T | 2008 | Muscle Nerve2008，37，2： | 1 |
| 9 | Biomechanical evaluation of four different posterior atlantoaxial fixation techniques显示文摘 | Crisco JJ 3rd Panjabi MM | 1992 | Spine1992，17，5： | 1 |
| 10 | Biomechanical evaluation of four different posterior atlanto - axial fixation techniques 显示文摘 | Dvorak J Panjabi MM | 1991 | Spine1991，17，： | 1 |
| 11 | Clinical experience with the Dynesys semirigid fixation system for the lumbar spine:surgical and patient-oriented outcome in 50 cases after an average of 2years显示文摘 | Grob D Benini A Junge A | 2005 | Spine2005，30，3： | 1 |
| 12 | Lifetime course of myasthenia gravis显示文摘 | Grob D Brenner N Namba T | 2008 | Muscle Nerve2008，37，2： | 1 |
| 13 | Clinical experience with the Dyne-sys semirigid fixation system for the lumbar spine: surgical and patient-oriented outcome in 50 cases after an average of 2 years 显示文摘 | Grob D Benini A Junge A | 2005 | Spine(Phila Pa 1976)2005，30，3： | 1 |
| 14 | Translaminar screws in the atlas显示文摘 | Floyd T Grob D | 2000 | Spine (Phila Pa 1976)2000，25，22： | 1 |
| 15 | Translaminar screw fixation of the lumbar and lumbosacral spine: A 5-year followup显示文摘 | HumkeT Grob D Dvorak J | 1998 | Spine1998，23，10： | 1 |
| 16 | Thermal design & verification of the EOS-AM1 nickel hydrogen batteries显示文摘 | Grob E W Chalmers D R Bennett C W etal | 1997 | Aerospace Power Systems and Technologies1997，439，： | 1 |
| 17 | Cotrel-Dubousset and Harfington instrumentation in idiopathic scoliosis:a comparison of long-term results显示文摘 | Humke T Grob D Seheier H | 1995 | Eur Spine J1995，4，5： | 1 |
| 18 | Purified bovine BMP extract and collagen for spine arthrodesis:preclinical safety and efficacy显示文摘 | Damien CJ Grob D Boden SD | 2002 | Spine2002，27，161： | 1 |
| 19 | Lumbar facet joint effusion in MRI: a sign of instability in degenerative spondylol sthes s? 显示文摘 | Lattig F Fekete T F Grob D | 2012 | EurSpineJ2012，21，2： | 1 |
| 20 | A prospective, cohort study comparing translaminar screw fixation with transforaminal lumbar interbody fusion and pedicle screw fixation for fusion of the degenerative lumbar spine 显示文摘 | Grob D Bartanusz V Jeszenszky D | 2009 | J Bone Joint Surg Br2009，91，10： | 1 |
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