|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Helicobacter pylori γ-glutamyl transpeptidase:A formidable virulence factor显示文摘Helicobacter pylori(H.pylori)produce an enzyme known asγ-glutamyl transpeptidase(HpGGT)that is highly conserved and common to all strains.HpGGT has been gaining increasing attention as an important virulence factor of the bacterium,having been demonstrated to be an important colonization factor in several animal models and has also recently been strongly associated with the development of peptic ulcer disease.From the results of various independent researcher groups,it is clear that HpGGT acts through several pathways to damage gastric epithelial cells including the induction of apoptosis and cell cycle arrest,production of reactive oxygen species leading to DNA damage,promotion of inflammation by increasing cyclooxygenase-2 and interleukin-8 expression,and upregulation of heparin-binding epidermal growth factor-like growth factor resulting in cell survival and proliferation.In addition,the potential role of HpGGT in promoting gastric carcinogenesis will also be discussed in this review.Apart from affecting the gastric epithelium,HpGGT also has immunomodulatory actions on host immune cells where it displays an antiproliferative effect on T cells by inducing cell cycle arrest and also works with other H.pylori virulence factors to skew dendritic cells towards a tolerogenic phenotype,possibly contributing to the persistence of the pathogen in the gastric mucosa. | Samantha Shi Min Ling Khay Guan Yeoh Bow Ho | 2013 | World Journal of Gastroenterology2013，19，45： | 10 |
| 2 | Stability of randomly amplified polymorphic DNA fingerprinting in genotyping clinical isolates of Helicobacter pylori显示文摘AIM: Hpylorigenomes are highly diversified. This project was designed to genotype Hpyloriisolates by the polymerase chain reaction (PCR)-based randomly amplified polymorphic DNA (RAPD) fingerprinting technique and to verify its stability by Southern blotting and DNA sequencing.METHODS: Clinical isolates of Hpyloriwere cultured from gastric antra and cardia of 73 individuals, and genomic DNA was prepared for each isolate. RAPD was carried out under optimized conditions. 23S rDNA was regarded as an internal control, and a 361 bp rDNA fragment (RDF) was used as a probe to screen the RAPD products by Southern blotting.Ten RDFs from different clinical isolates and the flanking regions (both upstream and downstream) of four RDFs were amplified and sequenced.RESULTS: Hpyloriisolates from different individuals had different RAPD profiles, but the profiles for isolates cultured from different gastric sites of a given individual were identical in all but one case. Isolates from 27 individuals were RDF positive by Southern blotting. Sequences of the RDFs and their flanking regions were almost the same between the RDF positive and negative isolates as determined by Southern blotting. There was no binding site for random PCR primer inside the sequences.CONCLUSION: RAPD is very useful in genotyping H pylori grossly on a large scale. However, it seems unstable in amplification of low yield fragments, especially those that do not appear as visible bands on the agarose gel stained with EB, since the palmer is partially matched to the template. | Feng-Chan Han Han-Chong Ng Bow Ho Department of Microbiology,Faculty of Medicine,National University of Singapore,5 Science Drive 2,Singapore 117595,Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，9： | 9 |
| 3 | Helicobacter pylori acquisition of metronidazole resistance by natural transformation in vitro显示文摘INTRODUCTIONGenetictransformationisaprocesbywhichaceltakesupnakedDNAfromthesurroundingmediumandincorporatesitintoitsowngenomi... | HUA Jiesong 1, ZHENG Peng Yuan 1, Teo Keng Fong 1, Khin Mar Mar 2 and HO Bow 1 | 1998 | World Journal of Gastroenterology1998，4，5： | 8 |
| 4 | Prevalence of primary Helicobacter pylori resistance to metronidazole and clarithromycin in Singapore显示文摘INTRODUCTIONEradication of Helicobacter pylori,a bacteriumresiding in stomach and causing peptic ulcer disease,can be achieved by using combination therapiesconsisting of one or two antibiotics with a protonpump inhibitor (PPI).The major antibiotics widelyused in the regimens to eradicate H.pylori aremetronidazole and clarithromycin.However,resistance to these antibiotics by H.pylori | Hua JS Bow H Zheng PY Khay-Guan Y | 2000 | World Journal of Gastroenterology2000，6，1： | 7 |
| 5 | Species differentiation and identification in the genus of Helicobacter显示文摘Asearlyasnineteencentury,incidentalpresenceofspiralorganismswasnotedinthestomachsofdogs[1],ratsandcats[2].Intheearlyyearsofth... | HUA Jie Song, ZHENG Peng Yuan and HO Bow | 1999 | World Journal of Gastroenterology1999，5，1： | 5 |
| 6 | Identification of H.pyloristrain specific DNA sequences between two clinical isolates from NUD and gastric ulcer by SSH显示文摘AIM: The genomes of Helicobacter pylori(H. pylori) from different individuals are different. This project was to identify the strain specific DNA sequences between two clinical H. pylori isolates by suppression subtractive hybridization (SSH).METHODS: Two clinical H. pylori isolates, one from gastric ulcer (GU, tester) and the other from non-ulcer dyspepsia (NUD, driver), were cultured and the genomic DNA was prepared and submitted to AluⅠdigestion. Then two different adaptors were ligated respectively to the 5′-end of two aliquots of the tester DNA fragments and SSH was made between the tester and driver DNA. The un-hybridized tester DNA sequences were amplified by two sequential PCR and cloned into pGEM-T-Easy Vector. The tester strain specific inserts were screened and disease related DNA sequences were identified by dot blotting.RESULTS: Among the 240 colonies randomly chosen, 50contained the tester strain specific DNA sequences. Twenty three inserts were sequenced and the sizes ranged from 261 bp to 1 036 bp. Fifteen inserts belonged to the H.pylori plasmid pHPO100 that is about 3.5 kb and codes a replication protein A. Other inserts had patches of homologous to the genes of H. pylori in GenBank. Various patterns of dot blots were given and no GU strain unique DNA sequences were found when 4 inserts were used as probes to screen the genomic DNA from 27 clinical isolates, 8 from GU, 12 from duodenum ulcer (DU), 4 from GU-DU, 2 from NUD and 1from gastric cancer (GC). But a 670 bp DNA fragment (GU198)that was a bit homologous to the 3′-end of the gene of thymidylate kinase was positive in 7 GU strains (7/8), 3 GUDU strains (3/4) and 3 DU strains (3/12). A 384 bp fragment (GU79) of the replication gene A (repA) was positive only in 4 H, pylori isolates, 2 from GU and 2 from GU-DU.CONCLUSION: Differences exist in the genes of different H.pylori isolates. SSH is very effective to screen H. pylori strain specific DNA sequences between two clinical isolates,and some of these sequences may have clinical significance. | Feng-Chan Han Min Gong Han-Chong Ng Bow Ho, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117595, Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，8： | 5 |
| 7 | Fifth metatarsal fractures and current treatment显示文摘Metatarsal fractures are one of the most common injuries of the foot. There has been conflicting literatureon management of fifth metatarsal fractures due to inconsistency with respect to classification of these fractures. This article provides a thorough review of fifth metatarsal fractures with examination of relevant literature to describe the management of fifth metatarsal fractures especially the proximal fracture. A description of nonoperative and operative management for fifth metatarsal fractures according to anatomical region is provided. | Julia Bowes Richard Buckley | 2016 | World Journal of Orthopedics2016，7，12： | 3 |
| 8 | Helicobacter pylori γ-Glutamyl Transpeptidase Is a Pathogenic Factor in the Development of Peptic Ulcer Disease显示文摘 | Min Gong Samantha Shi Min Ling Sook Yin Lui Khay Guan Yeoh Bow Ho | 2010 | Gastroenterology2010，，2： | 3 |
| 9 | A rat model of bone cancer pain显示文摘 | S.J Medhurst K Walker M Bowes B.L Kidd M Glatt M Muller M Hattenberger J Vaxelaire T O’Reilly G Wotherspoon J Winter J Green L Urban | 2002 | Pain2002，，： | 2 |
| 10 | Coexistence of Helicobacter pylori spiral and coccoid forms in experimental mice显示文摘AbstractAIMToinfectmicewithHelicobacterpylorianddetectimmuneresponseagainsttwoformsofH.pylori.METHODSAnisolateofH.pyloriobtai... | HUA Jiesong 1, HO Bow 1, ZHENG Pengyuan 1, YEOH Khay Guan 2, NG Han Chong 1 and LIM Seng Gee 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 2 |
| 11 | A system for interpretation of line drawings显示文摘 | BOW S T | 1990 | IEEE Transactions on Pattern Analysis and Machine Intelligence1990，12，10： | 1 |
| 12 | Simple Microprocessor Implementation of New Regular-Sampled Harmonic Elimination PWM Techniques显示文摘 | Bowes S R Clark P R | 1992 | IEEE Trans IA1992，28，1： | 1 |
| 13 | Regular-sampled harmonic-elimination PWM con- trol of inverter Drive显示文摘 | BOWES S R | 1995 | IEEE Transactions on Power Elec- tronics1995，10，5： | 1 |
| 14 | Comparison of the photosynthetic characteristics of three submersed aquatic plants 显示文摘 | Van T K Haller W T Bowes G | 1976 | Plant Physiology1976，58，： | 1 |
| 15 | Facing the inevitable: Plants and increasing atmospheric CO2 显示文摘 | Bowes G | 1993 | Ann Rev Plant Physiol Plant Mol Biol1993，44，： | 1 |
| 16 | Project evaluation in the presence of multiple embedded real options-evidence from the Taiwan high-speed rail project显示文摘 | Bowe M Lee D L | 2004 | Journal of Asian Economics2004，15，： | 1 |
| 17 | Intravenous and oral itraconazole versus intravenous amphotericin B deoxycholate as empirical antifungal therapy for persistent fever in neutropenic patients with cancer who are receiving broad spectrum antibacterial therapy: a randomized, controlled trial显示文摘 | Boogaerts M Winston DJ Bow EJ | 2001 | Ann Intern Med2001，735，6： | 1 |
| 18 | Comparison of a trialof labor with an elective second Cesarean section显示文摘 | McMahon MJ Luthier ER Bowes WA | 1996 | N Engl JMed1996，335，6： | 1 |
| 19 | Antimicrobial prophylaxis and outpatient management of fever and neutropenia in adults treated for malignancy:American society of clinical oncology clinical practice guideline显示文摘 | Flowers CR Seidenfeld J Bow EJ | 2013 | J Clin Oncol2013，31，6： | 1 |
| 20 | Differential role of cortieotrophin-relcasing factor receptor types Ⅰ and 2 in stressinduced suppression of pulsatile luteinising hormone secretion in the female rat显示文摘 | Li XF Bowe JE Kinsey-Jones J | | 0，，8： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员