|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Helicobacter pylori γ-glutamyl transpeptidase:A formidable virulence factor显示文摘Helicobacter pylori(H.pylori)produce an enzyme known asγ-glutamyl transpeptidase(HpGGT)that is highly conserved and common to all strains.HpGGT has been gaining increasing attention as an important virulence factor of the bacterium,having been demonstrated to be an important colonization factor in several animal models and has also recently been strongly associated with the development of peptic ulcer disease.From the results of various independent researcher groups,it is clear that HpGGT acts through several pathways to damage gastric epithelial cells including the induction of apoptosis and cell cycle arrest,production of reactive oxygen species leading to DNA damage,promotion of inflammation by increasing cyclooxygenase-2 and interleukin-8 expression,and upregulation of heparin-binding epidermal growth factor-like growth factor resulting in cell survival and proliferation.In addition,the potential role of HpGGT in promoting gastric carcinogenesis will also be discussed in this review.Apart from affecting the gastric epithelium,HpGGT also has immunomodulatory actions on host immune cells where it displays an antiproliferative effect on T cells by inducing cell cycle arrest and also works with other H.pylori virulence factors to skew dendritic cells towards a tolerogenic phenotype,possibly contributing to the persistence of the pathogen in the gastric mucosa. | Samantha Shi Min Ling Khay Guan Yeoh Bow Ho | 2013 | World Journal of Gastroenterology2013，19，45： | 10 |
| 2 | Stability of randomly amplified polymorphic DNA fingerprinting in genotyping clinical isolates of Helicobacter pylori显示文摘AIM: Hpylorigenomes are highly diversified. This project was designed to genotype Hpyloriisolates by the polymerase chain reaction (PCR)-based randomly amplified polymorphic DNA (RAPD) fingerprinting technique and to verify its stability by Southern blotting and DNA sequencing.METHODS: Clinical isolates of Hpyloriwere cultured from gastric antra and cardia of 73 individuals, and genomic DNA was prepared for each isolate. RAPD was carried out under optimized conditions. 23S rDNA was regarded as an internal control, and a 361 bp rDNA fragment (RDF) was used as a probe to screen the RAPD products by Southern blotting.Ten RDFs from different clinical isolates and the flanking regions (both upstream and downstream) of four RDFs were amplified and sequenced.RESULTS: Hpyloriisolates from different individuals had different RAPD profiles, but the profiles for isolates cultured from different gastric sites of a given individual were identical in all but one case. Isolates from 27 individuals were RDF positive by Southern blotting. Sequences of the RDFs and their flanking regions were almost the same between the RDF positive and negative isolates as determined by Southern blotting. There was no binding site for random PCR primer inside the sequences.CONCLUSION: RAPD is very useful in genotyping H pylori grossly on a large scale. However, it seems unstable in amplification of low yield fragments, especially those that do not appear as visible bands on the agarose gel stained with EB, since the palmer is partially matched to the template. | Feng-Chan Han Han-Chong Ng Bow Ho Department of Microbiology,Faculty of Medicine,National University of Singapore,5 Science Drive 2,Singapore 117595,Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，9： | 9 |
| 3 | Helicobacter pylori acquisition of metronidazole resistance by natural transformation in vitro显示文摘INTRODUCTIONGenetictransformationisaprocesbywhichaceltakesupnakedDNAfromthesurroundingmediumandincorporatesitintoitsowngenomi... | HUA Jiesong 1, ZHENG Peng Yuan 1, Teo Keng Fong 1, Khin Mar Mar 2 and HO Bow 1 | 1998 | World Journal of Gastroenterology1998，4，5： | 8 |
| 4 | Species differentiation and identification in the genus of Helicobacter显示文摘Asearlyasnineteencentury,incidentalpresenceofspiralorganismswasnotedinthestomachsofdogs[1],ratsandcats[2].Intheearlyyearsofth... | HUA Jie Song, ZHENG Peng Yuan and HO Bow | 1999 | World Journal of Gastroenterology1999，5，1： | 5 |
| 5 | Identification of H.pyloristrain specific DNA sequences between two clinical isolates from NUD and gastric ulcer by SSH显示文摘AIM: The genomes of Helicobacter pylori(H. pylori) from different individuals are different. This project was to identify the strain specific DNA sequences between two clinical H. pylori isolates by suppression subtractive hybridization (SSH).METHODS: Two clinical H. pylori isolates, one from gastric ulcer (GU, tester) and the other from non-ulcer dyspepsia (NUD, driver), were cultured and the genomic DNA was prepared and submitted to AluⅠdigestion. Then two different adaptors were ligated respectively to the 5′-end of two aliquots of the tester DNA fragments and SSH was made between the tester and driver DNA. The un-hybridized tester DNA sequences were amplified by two sequential PCR and cloned into pGEM-T-Easy Vector. The tester strain specific inserts were screened and disease related DNA sequences were identified by dot blotting.RESULTS: Among the 240 colonies randomly chosen, 50contained the tester strain specific DNA sequences. Twenty three inserts were sequenced and the sizes ranged from 261 bp to 1 036 bp. Fifteen inserts belonged to the H.pylori plasmid pHPO100 that is about 3.5 kb and codes a replication protein A. Other inserts had patches of homologous to the genes of H. pylori in GenBank. Various patterns of dot blots were given and no GU strain unique DNA sequences were found when 4 inserts were used as probes to screen the genomic DNA from 27 clinical isolates, 8 from GU, 12 from duodenum ulcer (DU), 4 from GU-DU, 2 from NUD and 1from gastric cancer (GC). But a 670 bp DNA fragment (GU198)that was a bit homologous to the 3′-end of the gene of thymidylate kinase was positive in 7 GU strains (7/8), 3 GUDU strains (3/4) and 3 DU strains (3/12). A 384 bp fragment (GU79) of the replication gene A (repA) was positive only in 4 H, pylori isolates, 2 from GU and 2 from GU-DU.CONCLUSION: Differences exist in the genes of different H.pylori isolates. SSH is very effective to screen H. pylori strain specific DNA sequences between two clinical isolates,and some of these sequences may have clinical significance. | Feng-Chan Han Min Gong Han-Chong Ng Bow Ho, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117595, Republic of Singapore | 2003 | World Journal of Gastroenterology2003，9，8： | 5 |
| 6 | Helicobacter pylori γ-Glutamyl Transpeptidase Is a Pathogenic Factor in the Development of Peptic Ulcer Disease显示文摘 | Min Gong Samantha Shi Min Ling Sook Yin Lui Khay Guan Yeoh Bow Ho | 2010 | Gastroenterology2010，，2： | 3 |
| 7 | Coexistence of Helicobacter pylori spiral and coccoid forms in experimental mice显示文摘AbstractAIMToinfectmicewithHelicobacterpylorianddetectimmuneresponseagainsttwoformsofH.pylori.METHODSAnisolateofH.pyloriobtai... | HUA Jiesong 1, HO Bow 1, ZHENG Pengyuan 1, YEOH Khay Guan 2, NG Han Chong 1 and LIM Seng Gee 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 2 |
| 8 | Variants in linkage disequilibrium with the late cornified envelope gene cluster deletion are associated with susceptibility to psoriatic arthritis显示文摘 | Bowes J Flynn E Ho P | 2010 | Ann Rheum Dis2010，69，12： | 1 |
| 9 | Agglutination of Helicobacter pylori coccoids by lectins显示文摘AIM To study the agglutination pattern ofHelicobacter pylori coccoid and spiral forms.METHODS Assays of agglutination andagglutination inhibition were applied usingfifteen commercial lectins.RESULTS Strong agglutination was observedwith mannose-specific Concanavalin A(Con A),fucose-specific Tetragonolobus purpureas(Lotus A)and N-acetyl glucosamine-specificTriticum vulgaris(WGA)lectins.Mannose andfucose specific lectins were reactive with allstrains of H.pylori coccoids as compared to thespirals.Specific carbohydrates,glycoproteinsand mucin were shown to inhibit H.pylorilectin-agglutination reactions.Pre-treatment ofthe bacterial cells with formalin and sulphuricacid did not alter the agglutination patterns withlectins.However,sodium periodate treatment ofbacterial cells were shown to inhibitagglutination reaction with Con A,Lotus A andWGA lectins.On the contrary,enzymatictreatment of coccoids and spirals did not showmarked inhibition of H.pylori-lectinagglutination.Interestingly,heating of H.pylori cells at 60℃ for 1 hour was shown toaugment the agglutination with all of the lectinstested.CONCLUSION The considerable differences inlectin agglutination patterns seen among the twodifferentiated forms of H.pylori might beattributable to the structural changes during theevents of morphological transformation,resulting in exposing or masking some of the sugar residues on the cell surface.Possibility ofvarious sugar residues on the cell wall of thecoccoids may allow them to bind to differentcarbohydrate receptors on gastric mucus andepithelial cells.The coccoids with adherencecharacteristics like the spirals could aid in thepathogenic process of Helicobacter infection.This may probably lead to different clinicaloutcome of H.pylori associated gastroduodenaldisease. | Mar Mar Khin Jie Song Hua Han Cong Ng Torkel Wadstrm Bow Ho | 2000 | World Journal of Gastroenterology2000，6，2： | 1 |
| 10 | Variants in linkage disequilibrium with the late cornified envelope gene cluster deletion are associated with susceptibility to psoriatic arthritis显示文摘 | Bowes J Flynn E Ho P | 2010 | Ann Rheum Dis2010，69，12： | 1 |
| 11 | Serum IgG response to differentiated antigens of Helicobacter pylori显示文摘SerumIgGresponsetodiferentiatedantigensofHelicobacterpyloriHUAJieSong1,KHINMarMar1,ZHENGPengYuan1,YEOHKhayGuan2,NgHanChon... | HUA Jie Song 1, KHIN Mar Mar 1, ZHENG Peng Yuan 1, YEOH Khay Guan 2, Ng Han Chong 1 and HO Bow 1 | 1998 | World Journal of Gastroenterology1998，4，3： | 1 |
| 12 | Comprehensive assessment of rheumatoid arthritis susceptibility loci in a large psori- atic arthritis cohort显示文摘 | Bowes J Ho P Flynn E | 2012 | Ann Rheum Dis2012，71，8： | 1 |
| 13 | Recombinant COS- 1 cells express Carcinoscorpius rout ndicauda Factor C 显示文摘 | Roopashree SD Bow Ho Jeak LD | 1997 | Biotechnology Letters1997，19，4： | 1 |
| 14 | A new era in pyrogen testing显示文摘 | i Jeak Ding Bow Ho | 2001 | TRENDS in Biotechnology2001，19，8： | 1 |
| 15 | Helicobacter pylori γ-Glutamyl Transpeptidase Is a Pathogenic Factor in the Development of Peptic Ulcer Disease显示文摘 | Min Gong Samantha Shi Min Ling Sook Yin Lui Khay Guan Yeoh Bow Ho | 2010 | Gastroenterology2010，，2： | 1 |
| 16 | Comprehensive assessment of eheu- matoid arthritis susceptibility loci in a large psoriatic arthritis cohort 显示文摘 | Bowes J Ho P Flynn E | 2012 | Ann Rheum Dis2012，71，8： | 1 |
| 17 | Prominent role of y-glutamyl-transpeptidase on the growth of Helicobacter pylori显示文摘AIM: γ-glutamyl transpeptidase (GGT) has been reported as a virulence and colonizing factor of Helicobacter pylori (Hpylori). This study examined the effect of GGT on thegrowth of H pylori. METHODS: Standard H pylori strain NCTC 11637 and 4 dinical isolates with different levels of GGT activity as measured by an enzymatic assay were used in this study. Growth inhibilJon and stimulation studies were carried out by culturing H pyloriin brain heart infusion broth supplemented with specific GGT inhibitor (L-serine sodium borate complex, SBC) or enhancer (glutathione together with glycyl-glycine), respectively. The growth profiles of Hpyloriwere determined based on viable bacterial count at time interval. RESULTS: Growth was more profuse for Hpyloriisolates with higher GGT activity than those present with lower GGT activity. However, in the presence of SBC, growth of Hpylori was retarded in a dose dependent manner (P = 0.034). In contrast, higher growth rate was observed when GGT activity was enhanced in the presence of glutathione and glycyl-glycine. CONCLUSION: Higher GGT activity provides an advantage to the growth of Hpy/oriin vitro. Inhibition of GGT activity by SBC resulted in growth retardation. The study shows that GGT plays an important role on the growth of Hpy/ori. | Bow Ho | 2004 | World Journal of Gastroenterology2004，10，20： | 0 |
| 18 | Effect of biopsies on sensitivity and specificity of ultra-rapid urease test for detection of Helicobacter pylori infection:A prospective evaluation显示文摘AIM: To prospectively assess the sensitivity, specificity and time to positivity of theUItra-rapid urease test (URUT) for Helicobacter pylori ( H pylori ), and compare the results of one with those of two biopsies.METHODS: Five antral biopsies were taken in consecutive patients undergoing upper endoscopy: one and two biopsies for URUT, and one each for H pylori culture and histology.URUT was read at 1, 5, 10, 20 and 30 rain, 1, 2, 3 and 24 h after biopsy insertion into the reagent. A positive histology and/or culture was used as positive reference “gold standards”:RESULTS: URUT was more sensitive for detecting Hpy/ori with two biopsies rather than one, at all time points up to 120 rain. The sensitivity improved from 3.6% to 82.1% for one biopsy and 10.7% to 85.7% for two biopsies from 1 to 120 rain. The sensitivity reached 96.4% at 24 h for both,but the specificity reduced from 100% to 96% and 92% for one and two biopsies, respectively.CONCLUSION: Development of a positive URUT result is hastened by doubling the number of gastric biopsies. We recommend taking two instead of one biopsy to achieve an earlier positive URUT result so that H pylorieradication therapy can be initiated before patient is discharged from the endoscopy suite. | LiLinLim KhekYuHo Bow Ho Manuel Salto-Tellez | 2004 | World Journal of Gastroenterology2004，10，13： | 0 |
| 19 | Validation of four Helicobacter pylori rapid blood tests in a multi-ethnic Asian population显示文摘AIM: To validate the accuracy of four rapid blood tests in the diagnosis of Helicobacter pylori.METHODS: Consecutive dyspeptic patients scheduled for endoscopy at the National University Hospital,Singapore, were interviewed and had blood drawn for serology. The first 109 patients were tested with BM-test (BM), Pyloriset Screen (PS) and QuickVue (QV), and the next 99 subjects were tested with PS and Unigold (UG).Endoscopies were performed blinded to rapid blood test results and biopsies were taken for culture and rapid urease test. Urea breath tests were performed after endoscopies. The rapid blood test results were compared with four reference tests (rapid urease test, culture,serology, and breath test).RESULTS: The study population composed of 208patients (mean age 43.1 years; range 18-73 years; 119males; 174 Chinese). The number of evaluable patientsfor BM, QV, UG and PS were 102, 102, 95, and 197,respectively. The sensitivity and specificity, respectively were: PS 80.2%, 95.8%; UG 55.9%, 100%; QV 43.3%,100%; BM 67.2%, 97.1%.CONCLUSION: The rapid blood test kits showed high specificity and positive predictive value (97-100%), while sensitivity and negative predictive value ranged widely (43%-80% and 47%-73%, respectively). Among test kits, PS showed the best sensitivity (80%), best negative predictive value (73%) and best negative likelihood ratio (0.207). PS had a specificity of 96%, positive predictive value of 97% and positive likelihood ratio of 19.1. | Lee-Guan Lim Khay-Guan Yeoh Bow Ho Seng-Gee Lim | 2005 | World Journal of Gastroenterology2005，11，42： | 0 |
| 20 | The Molecular Mechanism of Interaction between Sushi Peptide and Pseudomonas Endotoxin显示文摘腐败吃惊被克否定的细菌的感染引起。Lipopolysaccharide (LPS ) 是在克否定的细菌的外部膜上在场的 bioactive 分子。LPS 在宿主细胞膜上与传感器交往激活导致象 TNF- 那样的 cytokines 的生产过剩的细胞内部的发信号小径,这通常被认为。这原因发炎并且最终,腐败吃惊。类脂化合物 A 是 LPS 分子的 pharmacophore。因此,开发能够在高亲密关系的有约束力的 LPS 的简历分子,特别到一半是的类脂化合物抵销 LPS 毒性的一个有效方法。因素 C,在马蹄铁螃蟹 ameobocytes 的丝氨酸朊酶,是敏感的跟踪 LPS 的层次。我们从因素 C 的 LPS 有约束力的领域导出寿司肽。我们的更早的学习证明寿司肽禁止导致 LPS 腐败在 mice.Here 的吃惊,我们证明在 LPS 和寿司之间的分子的相互作用 1 肽被在 LPS 和寿司的类脂化合物尾巴之间的恐水病的相互作用支持 1 肽。面对 LPS,而且,肽从随机的结构转移进螺旋状的符合构造,因此,它破坏 LPS 总数抵销 LPS 毒性。 | Peng Li Miao Sun Thorsten Wohland Bow Ho Jeak Ling Ding | 2006 | Cellular & Molecular Immunology2006，3，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员