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| 1 | Trends of foodborne diseases in China: lessons from laboratory- based surveillance since 2011显示文摘Foodborne 疾病是世界范围的最重要的公共健康问题之一。中国在食物链的所有方面面对各种各样、崭新的挑战。从从 2013 ~ 2016 的基于实验室的 foodborne 疾病监视系统的数据,以及不同区域和年龄,能在与多样的特征检测的病原体的模式与差别一起被发现。Vibrio parahaemolyticus 是在中国的传染腹泻的领先的原因,特别在在沿海的区域的成年人之中。沙门氏菌是为实质的社会经济的负担负责的严肃、广泛地分布式的病原体。Shigella 主要在 5 年下面在孩子之中与开发更少的区域在西北中国和内陆省(河南省) 被识别。从从 2011 ~ 2016 报导系统的 foodborne 疾病爆发的数据证明有毒的动物和为大多数死亡负责的植物因素在在西南区域的遥远的区域是有毒的蘑菇(54.7%) 。引起了报导的大多数案例(42.3%) 的生物危险被归因于 V。parahaemolyticus, foodborne 爆发的领先的原因。在这评论,我们在中国总结最近的监视途径到 foodborne 疾病并且在发达国家把结果与那些作比较。 | Jikai Liu Li Bai Weiwei Li Haihong Han Ping Fu Xiaochen Ma Zhenwang Bi Xiaorong Yang Xiuli Zhang Shiqi Zhen Xiaoling Deng Xiumei Liu Yunchang Guo | 2018 | Frontiers of Medicine2018，12，1： | 55 |
| 2 | Lime and Phosphate Could Reduce Cadmium Uptake by Five Vegetables Commonly Grown in South China显示文摘A pot experiment was conducted in artificially Cd-contaminated (5 mg Cd kg-1) soils to investigate the feasibility of using lime (3 g kg-1) or phosphate (80 mg P kg-1) to mitigate uptake of Cd by vegetables.Five common vegetables in South China,including lettuce (Lactuca sativa L.var.ramosa Hort.),Chinese cabbage [Brassica rapa L.subsp.Chinensis (L.) var.parachinensis (L.H.Bailey) Hanect],Chinese broccoli (Brassica oleracea L.var.albiflora Kuntze),white amaranth (Amaranthus tricolor L.) and purslane (Amaranthus viridis L.),were grown in the soils and harvested after 60 d.The results showed that liming significantly reduced Cd uptake by most vegetables by 40%-50% (or a maximum of 70%),mainly due to immobilization of soil Cd.Increased availability of Ca in the soil might also contribute to the Cd uptake reduction as a result of absorption competition between Ca and Cd.Liming caused biomass reduction in white amaranth and purslane,but did not influence growth of the other vegetables.Phosphate decreased Cd uptake by vegetables by 12%-23%.Compared with lime,phosphate decreased,to a smaller extent,the bioavailability of Cd in most cases.Phosphate markedly promoted growth of vegetables.Changes in soil chemistry by adding lime or phosphate did not markedly influence nutrient uptake of vegetables except that lime increased Ca content and phosphate increased P content in shoots of the vegetables.The results suggested that a proper application of lime could be effective in reducing Cd uptake of vegetables,and phosphate could promote growth of the vegetables as well as alleviate the toxicity of Cd. | TAN Wan-Neng LI Zhi-An QIU Jing ZOU Bi LI Ning-Yu ZHUANG Ping WANG Gang | 2011 | Pedosphere2011，21，2： | 34 |
| 3 | Morphological study on colonic pathology in ulcerative colitis treated by moxibustion显示文摘AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine group.Thirtypatients were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the treatment.Mucin was also analyzed by H.E and AB-PASstaining.RESULTS Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion group.In the control group,5patients were clinically cured,7 improved and 4unchanged.Thirteen patients with active UCwere taken as the subjects for histopathologicanalysis in this study.The colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also changed.Inmost sections,the chronic inflammation ofmucosa was geatly ameliorated(P<0.01).Theinflammatory cell infiltratation much decreased and neutrophils,disapeared in most sections(P<0.001).The goblet cells significantlyincreased(P<0.001);crypt paracrypt abscessor mucosal ulceration was seen(P<0.001).CONCLUSION The rate of cure of ulcerative colitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal. | Huan Gan Wu Li Bin Zhou Da Ren Shi Shi Min Liu Hui Rong Liu Bi Meng Zhang Han Ping Chen Lin Shan Zhang | 2000 | World Journal of Gastroenterology2000，6，6： | 28 |
| 4 | Heavy Metal Contamination in Soil and Soybean near the Dabaoshan Mine, South China显示文摘Concentrations of Pb, Cd, Cu, Zn, Cr and Ni in soybean (Glycine max L.) grown near the Dabaoshan Mine were investigated, and their potential risk to the health of inhabitants was estimated. In the Fandong (FD) and Zhongxin (ZX) villages, which are near the Dabaoshan mineral deposit, concentrations of Pb (0.34 mg kg^(-1) for FD), Cd (0.23 mg kg^(-1) for ZX) and Cr (1.14 and 1.75 mg kg^(-1) for FD and ZX, respectively) in the seeds of soybean exceeded the tolerance limit set by Chinese standards. The estimated daily intakes (EDIs) from consumption of soybean seeds for FD inhabitants were 0.570, 0.170, 38.550, 142.400, 1.910 and 14.530 μg d^(-1) kg^(-1) boby weight for Pb, Cd, Cu, Zn, Cr and Ni, respectively. Our results indicate that soybeans grown in the vicinity of the Dabaoshan Mine accumulate some metals, and the seeds pose a potential health risk to the local inhabitants. | ZHUANG Ping LI Zhi-An ZOU Bi XIA Han-Ping WANG Gang | 2013 | Pedosphere2013，23，3： | 23 |
| 5 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 6 | A new iridoid glycoside from Gardenia jasminoides显示文摘新 iridoid glycoside, 6 ′ - O-sinapoylgeniposide,从栀子 jasminoides 埃利斯被孤立,它的结构根据 1D 和 2D NMR 被阐明, HR-ESI-MS 技术。 | Xiao Qin Zhou Zhi Ming Bi Ping Li Dan Tang Hal Xia Cai | 2007 | Chinese Chemical Letters2007，18，10： | 10 |
| 7 | On the Radiative Properties of Ice Clouds:Light Scattering, Remote Sensing,and Radiation Parameterization显示文摘Presented is a review of the radiative properties of ice clouds from three perspectives: light scattering simulations, remote sensing applications, and broadband radiation parameterizations appropriate for numerical models. On the subject of light scattering simulations, several classical computational approaches are reviewed, including the conventional geometric-optics method and its improved forms, the finite-difference time domain technique, the pseudo-spectral time domain technique, the discrete dipole approximation method, and the T-matrix method, with specific applications to the computation of the singlescattering properties of individual ice crystals. The strengths and weaknesses associated with each approach are discussed.With reference to remote sensing, operational retrieval algorithms are reviewed for retrieving cloud optical depth and effective particle size based on solar or thermal infrared(IR) bands. To illustrate the performance of the current solar- and IR-based retrievals, two case studies are presented based on spaceborne observations. The need for a more realistic ice cloud optical model to obtain spectrally consistent retrievals is demonstrated. Furthermore, to complement ice cloud property studies based on passive radiometric measurements, the advantage of incorporating lidar and/or polarimetric measurements is discussed.The performance of ice cloud models based on the use of different ice habits to represent ice particles is illustrated by comparing model results with satellite observations. A summary is provided of a number of parameterization schemes for ice cloud radiative properties that were developed for application to broadband radiative transfer submodels within general circulation models(GCMs). The availability of the single-scattering properties of complex ice habits has led to more accurate radiation parameterizations. In conclusion, the importance of using nonspherical ice particle models in GCM simulations for climate studies is proven. | Ping YANG Kuo-Nan LIOU Lei BI Chao LIU Bingqi YI Bryan A.BAUM | 2015 | Advances in Atmospheric Sciences2015，32，1： | 9 |
| 8 | Mitochondrial Modulation of Apoptosis Induced by Low-dose Radiation in Mouse Testicular Cells显示文摘Objective To investigate whether apoptosis induced by low-dose radiation(LDR) is regulated by mitochondrial pathways in testicular cells.Methods Male mice were exposed to whole-body LDR,and changes in mitochondrial function and in expression of apoptotic factors were analyzed in the testicular cells as follows.Total nitric-oxide synthase(T-NOS) and Na+/K+ATPase activities were biochemically assayed.Reactive oxygen species(ROS) and mitochondrial membrane potential(Δψm) were determined by flow cytometry using fluorescent probes.Levels of mRNAs encoding cytochrome c(Cyt c) and apoptosis-inducing factor(AIF) were quantified by real-time reverse-transcription PCR(RT-PCR).Expression of Cyt c,AIF,caspase-9,and caspase-3 at the proteinlevel was assessed by western blotting and immunohistochemistry.Results LDR induced an increase in T-NOS activity and ROS levels,and a decrease in Na+/K+ATPase activity and mitochondrial Δψm,in the testicular cells.The intensity of these effects increased with time after irradiation and with dose.The cells showed remarkable swelling and vacuolization of mitochondria,and displayed a time- and dose-dependent increase in the expression of Cyt c,AIF,procaspase-9,and procaspase-3.Activation of the two procaspases was confirmed by detection of the cleaved caspases.The changes in expression of the four apoptotic factors were mostly limited to spermatogonia and spermatocytes.Conclusion LDR can induce testicular cell apoptosis through mitochondrial signaling pathways. | FANG Fang GONG Ping Sheng ZHAO Hong Guang BI Yu Jing ZHAO Gang GONG Shou Liang WANG Zhi Cheng | 2013 | Biomedical and Environmental Sciences2013，26，10： | 9 |
| 9 | Distribution and Provenance of Detrital Minerals in Southern Coast of Shandong Peninsula显示文摘Detrital minerals of 137 offshore and 22 river sediment samples collected from Qingdao coastal areas have been analyzed. Four mineral assemblage provinces can be classified by Q-mode cluster analysis. Factor analysis identifies two major factors that account for the total variability in most common minerals: 1) based on the relationship of quartz, hornblende, actinolite, micas, and authigenic pyrite, 41.55% of the variability is related to sediment sources; 2) based on the relationship of epidote, garnet, sphere, and ilmenite, 23.21% can be related to strong hydrodynamic conditions that control transport and sedimentation. By comparing mineral compositions of river waters in the study area, the following four mineral provenances can be identified. The Qingdao-Laoshan nearshore area has a quartz-feldspar-epidote-hornblende-limenite-limonite-sphene assemblage, which is largely attributed to relict sediment and coastal erosion. The Jimo-Haiyang nearshore area has a quartz-feldspar-hornblende-epidote-limonite-mica-actinolite assemblage, derived largely from the Wulong River and Rushan River, and is also affected by the Huanghe River, while the Qianliyan Island area in the deeper offshore area separated by a mud belt has a similar assemblage. The Haiyang-Rushan nearshore area has a quartz-feldspar-hornblende-epidote-micas-limonite assemblage, indicating multiple sources from the Rushan River, the Wulong River, the Huanghe River, and coastal erosion. The central area, located in an eddy center, has a mica-authigenic pyrite-hornblende-quartz-feldspar assemblage, indicating multiple sources dominated by Huanghe River distal sediments. | LIU Jinqing YIN Ping ZHANG Yong SONG Hongying BI Shipu CAO Zhimin LIU Shanshan | 2017 | Journal of Ocean University of China2017，16，5： | 9 |
| 10 | Blood Pressure Associated with Arsenic Methylation and Arsenic Metabolism Caused by Chronic Exposure to Arsenic in Tube Well Water显示文摘Objective The effects of arsenic exposure from drinking water, arsenic metabolism, and arsenic methylation on blood pressure(BP) were observed in this study. Methods The BP and arsenic species of 560 participants were determined. Logistic regression analysis was applied to estimate the odds ratios of BP associated with arsenic metabolites and arsenic methylation capability. Results BP was positively associated with cumulative arsenic exposure(CAE). Subjects with abnormal diastolic blood pressure(DBP), systolic blood pressure(SBP), and pulse pressure(PP) usually had higher urinary i As(inorganic arsenic), MMA(monomethylated arsenic), DMA(dimethylated arsenic), and TAs(total arsenic) than subjects with normal DBP, SBP, and PP. The i As%, MMA%, and DMA% differed slightly between subjects with abnormal BP and those with normal BP. The PMI and SMI were slightly higher in subjects with abnormal PP than in those with normal PP. Conclusion Our findings suggest that higher CAE may elevate BP. Males may have a higher risk of abnormal DBP, whereas females have a higher risk of abnormal SBP and PP. Higher urinary i As may increase the risk of abnormal BP. Lower PMI may elevate the BP. However, higher SMI may increase the DBP and SBP, and lower SMI may elevate the PP. | WEI Bing Gan YE Bi Xiong YU Jiang Ping YANG Lin Sheng LI Hai Rong XIA Ya Juan WU Ke Gong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 9 |
| 11 | New C(21) steroidal glycoside from Cynanchum paniculatum显示文摘A new C21 steroidal glycoside, neocynapanogenin F 3-O-β-D-thevetoside 1, as well as its known aglycone neocynapanogenin F 2, which have an aberrant 13, 14:14, 15-disecopregnane-type skeleton were isolated from the root of Cynanchum paniculatum.Their structures were elucidated on the basis of spectroscopic data analysis. These compounds showed certain cytotoxic activities invitro. | Jing Dou Ping Li Zhi Ming Bi Jian Liang Zhou | 2007 | Chinese Chemical Letters2007，18，3： | 8 |
| 12 | Wide local excision could be considered as the initial treatment of primary anorectal malignant melanoma显示文摘背景肛门直肠的恶意的黑瘤是有极其差的预后的稀罕疾病。这研究的目的是探索临床的特征,诊断和肛门直肠的恶意的黑瘤的治疗策略。有肛门直肠的恶意的黑瘤的 57 个病人的数据被收集并且回顾地分析了的方法。直肠的流血和肛门质量被发现是肛门直肠的恶意的黑瘤的普通症状的结果。肛门直肠的恶意的黑瘤的外科手术前的诊断率是 48.6% 。全面 3 年、 5 年的幸存率分别地是 38.0% 和 21.3% 。舞台的 3 年的幸存率我和 II 病人分别地是 63.0% 和 16.7%(P=0.000 ) ,并且 5 年的幸存率是 33.3% 和 11.1%(P=0.001 ) ,它两个都有重要统计差别。病人的 3 年的幸存率经历的 abdmoninoperineal 切除术和病人经历的宽本地切除分别地是 36.7% 和 53.0%(P=0.280 ) 当 5 年的幸存率是 24.1% 和 23.1% 时(P=0.642 ) ,它两个都没有重要统计数值差别。这研究没识别幸存的结论在肛门直肠的恶意的黑瘤的治疗对切除术有利到 abdominoperineal,并且我们建议宽本地切除能被看作选择的起始的治疗。 | ZHOU Hai-tao ZHOU Zhi-xiang ZHANG Hai-zeng BI Jian-jun ZHAO Ping | 2010 | Chinese Medical Journal2010，，5： | 7 |
| 13 | Two new saponins from the aerial part of Astragalus membranaceus var. mongholicus显示文摘Two new saponins named mongholicoside A (1) and mongholicoside B (2) were isolated from the aerial part of Astragalus membranaceus var. mongholicus. Their structures were determined by 1D and 2D NMR, ESI-MS techniques and chemical methods. | Qing Tao Yu Ping Li Zhi Ming Bi Jun Luo Xiao Dan Gao | 2007 | Chinese Chemical Letters2007，18，5： | 7 |
| 14 | Grain-Size Distribution of Surface Sediments in the Bohai Sea and the Northern Yellow Sea: Sediment Supply and Hydrodynamics显示文摘The grain-size distribution of surface sediments in the Bohai Sea(BS) and the northern Yellow Sea(NYS), and its relationship with sediment supply and hydrodynamic environment were investigated based on grain-size compositions of surface sediments and modern sedimentation rates. The results showed that the surface sediments in the BS and the NYS were primarily composed of silty sand and clayey silt with a dominant size of silt. In addition, the Yellow River delivered high amount of water and sediments to the BS, and they are dominated in surface sediments(mainly silt) in the Bohai Bay, the Yellow River mouth, the center of the BS, and the north coast of Shandong Peninsula. The coarse-grained sediments were mainly deposited at the river mouth due to the estuarine filtration and physical sorting. Meanwhile, there was a significant relationship among the modern sedimentation rate, the surface sediment grain size distribution and sediment transport pattern. The areas with coarser surface sediments generally corresponded low sedimentation rates because of strong erosion;whereas the sedimentation rate was relatively high at the place that the surface sediments were fine-grained. Furthermore, the grain-size trend analysis showed that the areas with fine-grained surface sediments such as the mud area in the central BS and the upper Liaodong Bay were the convergent centers of surface sediments, except for the Bohai Bay and the subaqueous Yellow River Delta where offshore sediment transport was evident. | YUAN Ping WANG Houjie WU Xiao BI Naishuang | 2020 | Journal of Ocean University of China2020，19，3： | 6 |
| 15 | A Systematic Assessment of Blood Lead Level in Children and Associated Risk Factors in China显示文摘In this study, we searched multiple databases for all relevant original articles (1996-2013). To investigate blood lead levels (BLL) and possible risk factors for lead exposure among children in China A total of 388 articles met our inclusion criteria. The overall geometric mean (GM) BLL was 71 IJ.g/L, and the prevalence of elevated BLL (EBLL, defined as BLL 〉_100 Ixg/L) was l&.4&% among children. The prevalence of EBLL remained significantly higher among boys. In children less than 6 years of age, there were significantly increasing trends in both BLL and prevalence of EBLL in an age-dependent manner. The ban on leaded gasoline significantly reduced the BLL as well as EBLL prevalence; however, children whose parents had lower educational levels or were exposed to lead in the workplace had a higher EBLL prevalence. Despite its decline over time, the average BLL among children in China remains higher than the average level most recently reported in the United States. Childhood lead poisoning remains a public health problem in China. | WANG Lu LI Zhen HUANG Shao Xin DU Chuang WANG Hong HE Li Ping BI Yong Yi SHI Yong WANG Chun Hong | 2015 | Biomedical and Environmental Sciences2015，28，8： | 6 |
| 16 | Near-resonance enhanced label-free stimulated Raman scattering microscopy with spatial resolution near 130 nm显示文摘High-resolution optical microscopes that can break 180 nm in spatial resolution set to conventional microscopies are much-needed tools.However,current optical microscopes have to rely on exogenous fluorescent labels to achieve high resolution in biological imaging.Herein,we report near-resonance enhanced label-free stimulated Raman scattering(SRS)microscopy with a lateral resolution near 130 nm,in which the high-resolution image contrast originates directly from a low concentration of endogenous biomolecules,with sensitivity gains of approximately 23 times.Moreover,by using a 0.3-m-long optical fiber,we developed hyperspectral SRS microscopy based on spectral focusing technology.Attributed to enhancements in spatial resolution and sensitivity,we demonstrated highresolution imaging of three-dimensional structures in single cells and high-resolution mapping of large-scale intact mouse brain tissues in situ.By using enhanced high-resolution hyperspectral SRS,we chemically observed sphingomyelin distributed in the myelin sheath that insulates single axons.Our concept opens the door to biomedical imaging with~130 nm resolution. | Yali Bi Chi Yang Yage Chen Shuai Yan Guang Yang Yaozu Wu Guoping Zhang Ping Wang | 2018 | Light(Science & Applications)2018，7，1： | 4 |
| 17 | Comprehensive analysis of chemical constituents in Xingxiong injection by high performance liquid chromatography coupled with mass spectrometry显示文摘Xingxiong injection(XXI) is a widely used Chinese herbal formula prepared by the folium ginkgo extract and ligustrazine for the treatment of cardiovascular and cerebrovascular diseases. Compared with the pharmacological studies, chemical analysis and quality control studies on this formula are relatively limited. In the present study, a high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-QTOF MS) method was applied to comprehensive analysis of constituents in XXI. According to the fragmentation rules and previous reports, thirty ginkgo flavonoids, four ginkgo terpene lactones, and one alkaloid were identified. A high performance liquid chromatography coupled with triple quadrupole mass spectrometry(HPLC-QQQ MS) method was then applied to quantify ten major constituents in XXI. The method validation results indicated that the developed method had desirable specificity, linearity, precision and accuracy. The total contents of ginkgo flavonoids were about 22.05-25.51 μg·mL-1 and the ginkgo terpene lactones amounts were about 4.41-8.70 μg·m L-1 in six batches of XXI samples, respectively. Furthermore, cosine ratio algorithm and distance measurements were employed to evaluate the similarity of XXI samples, and the results demonstrated a high-quality consistency. This work could provide comprehensive information on the quality control of Xingxiong injection, which be helpful in the establishment of a rational quality control standard. | GUO Long DOU Li-Li DUAN Li LIU Ke BI Zhi-Ming LI Ping LIU E-Hu | 2015 | Chinese Journal of Natural Medicines2015，13，9： | 4 |
| 18 | A New Dimeric Phthalide from Angelica sinensis显示文摘A new dimeric phthalide named Z, Z’-3.3’a, 7.7’a-diligustilide was isolated from the roots of Angelica sinensis. Its structure was determined using spectroscopic methods and X-ray crystallographic diffraction analysis. | Ling YI Ping LI Zhi Ming BI | 2006 | Chinese Chemical Letters2006，17，12： | 4 |
| 19 | Establishment of a Sandwich ELISA Method for Detection of Vascular Endothelial Growth Factor in Serum Samples of Hepatocellular Carcinoma Patients显示文摘Objective To establish a sandwich ELISA method for detecting vascular endothelial growth factor (VEGF) in sera of population and the patients with hepatocellular carcinoma (HCC). Methods Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF proteins were expressed and purified from E. coli in His-Trx or GST fusion forms. The specific VEGF antibodies were elicited in experimental rabbits and mice by immunization of the full length VEGF fusion protein His-Trx-VEGF1-165. After purification of antibodies with chromatograph of Protein G, a sandwich ELISA technique was established. Serum VEGF levels were evaluated in 229 adults and 291 HCC patients. Results SDS-PAGE displayed that the molecular weights of the expressed full length (His-Trx-VEGF1-165), N-terminal (His-Trx-VEGF1-100) and C-terminal (GST-VEGF100-165) human VEGF fusion proteins were about 38KD, 31KD, and 33KD, respectively. Western blots confirmed that the prepared antisera were able to recognize both prokaryoticly and eukaryoticly expressed recombinant VEGF proteins. Assays of serially diluted His-Trx-VEGF1-100 by the established sandwich ELISA method showed that the linear range of the standard curve was 0.625-320 ng/mL, with the squared correlation coefficient R2=0.991. Screening of a serum panel containing 291 serum samples of HCC patients and 229 health adults revealed that the average VEGF level in HCC patients was higher than that in healthy controls, with a statically significant difference. Conclusion The established sandwich ELISA reflects the level of serum VEGF and provide scientific basis for screening metastasis and recurrence of HCC using serum VEGF as an index. | BING SHAN CHEN GAO JIAN-MING CHEN XIN-Yu BI BAO-YUN ZHANG YAN GUO CHEN-FANG DONG RUN AN QI SHI JING-QUN HU PING ZHAO JUN HAN XIAO-PING DONG | 2008 | Biomedical and Environmental Sciences2008，21，1： | 4 |
| 20 | Hepatitis B Immunoprophylactic Failure and Characteristics of the Hepatitis B Virus Gene in Mother-Infant Pairs in Parts of China显示文摘Objective To determine the hepatitis B immunoprophylactic failure rate in infants born to hepatitis B virus(HBV) infected mothers and to characterize HBV genes. Methods HBV-serological testing was conducted for pregnant women and infants. The complete genomes of 30 HBV isolates were sequenced, and genetic characteristics were analyzed using MEGA 5 software. Results The immunoprophylactic failure rate for infants who had completed the scheduled hepatitis B vaccination program was 5.76%(32/556). High sequence homology(99.8%-100%) was observed in 8 of the 10 mother-infant pairs. We identified 19 subgenotype C2 strains, 9 subgenotype B2 strains, and 2 subgenotype C1 strains. Three serotypes were detected: adr(19/30), adw(9/30), and ayw(2/30). The frequency of amino acid mutation of the ‘a' determinant region was 16.67%(5/30), including that of Q129 H, F134 Y, S136 Y, and G145 E. We detected 67 amino acid mutations in the basal core promoter, precore, and core regions of the genome. Conclusion The immunoprophylactic failure rate in infants born to HBV-infected mothers is low in the regions of China examined during this study. Moreover, HBV mutation in the ‘a' determinant region could not account for immunoprophylactic failure for all infants. | YIN Wen Jiao SHEN Li Ping WANG Fu Zhen ZHANG Guo Min ZHENG Hui WANG Feng LIU Tie Zhu MENG Qing Ling YI Yao CUI Fu Qiang BI Sheng Li | 2016 | Biomedical and Environmental Sciences2016，29，11： | 3 |
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