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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020,28,3: | 516 |
| 2 | Subtyping Animal Influenza Virus with General Multiplex RT-PCR and Liquichip High Throughput (GMPLex)显示文摘This study developed a multiplex RT-PCR integrated with luminex technology to rapidly subtype simultaneously multiple influenza viruses. Primers and probes were designed to amplify NS and M genes of influenza A viruses HA gene of H1, H3, H5, H7, H9 subtypes, and NA gene of the N1 and N2 subtypes. Universal super primers were introduced to establish a multiplex RT-PCR (GM RT-PCR). It included three stages of RT-PCR amplification, and then the RT-PCR products were further tested by LiquiChip probe, combined to give an influenza virus (IV) rapid high throughput subtyping test, designated as GMPLex. The IV GMPLex rapid high throughput subtyping test presents the following features: high throughput, able to determine the subtypes of 9 target genes in H1, H3, H5, H7, H9, N1, and N2 subtypes of the influenza A virus at one time; rapid, completing the influenza subtyping within 6 hours; high specificity, ensured the specificity of the different subtypes by using two nested degenerate primers and one probe, no cross reaction occurring between the subtypes, no non-specific reactions with other pathogens and high sensitivity. When used separately to detect the product of single GM RT-PCR for single H5 or N1 gene, the GMPLex test showed a sensitivity of 10-5(= 280ELD50) forboth tests and the Luminex qualitative ratio results were 3.08 and 3.12, respectively. When used to detect the product of GM RT-PCR for H5N1 strain at the same time, both showed a sensitivity of 10-4(=2800 ELD50). The GMPLex rapid high throughput subtyping test can satisfy the needs of influenza rapid testing. | Zhi-feng Qin Jie Sun Ti-kang Lu Shao-ling Zeng Qun-yi Hua Qing-yan Ling Shu-kun Chen Jian-qiang Lv Cai-hong Zhang Bing Cheng Zhou-xi Ruan Ying-zuo Bi Joseph J Giambrone Hong-zhuan Wu | 2012 | Virologica Sinica2012,27,2: | 8 |
| 3 | Requirement for cyclin D3 in germinal center formation and function显示文摘第二等的淋巴的纸巾的幼芽的中心(GC ) 对装高亲密关系的体液的有免疫力的回答批评。当多样化他们的抗体基因时,在 GC 以内的 B 房间经历快速的同种细胞的扩大和选择。尽管 GC B 房间采用一个唯一的 proliferative 程序提供这些进程,这通常被相信,很少联系 GC 的房间周期怎么被安排被知道。D 类型 cyclins 组成使房间能对生理的变化作出回应的房间周期引擎的一个重要部件。房间类型 -- 并且 D 类型 cyclins 的发展阶段特定的角色被描述了,但是 cyclin D 要求没在 GC 反应期间被探讨。在这研究,我们报导 cyclin D3 为切换的增长和 Ig 班大部分是非必需的在 vitro 激活的 B 房间。相反,在 Ccnd3 的 GC 开发 ?/ ?老鼠显著地被损害,作为是 T 房间依赖者抗体反应。在 GC 以内,尽管切换, unswitched B 房间被 cyclin D3 inactivation 影响, IgM?水池更严重地被减少。有趣地尽管有 cyclin D2 表示的补偿增加, Ccnd3 的一个重要数字 ?/ ?GC B 房间处于静止 G0 状态积累。最后,尽管 cyclin D3 inactivation 没在 GC B 房间破坏 BCL6 表示,支持 BCL6 overexpression 的效果完全堵住了 GC,建议 cyclin D3 调整 GC 形成的 BCL6 下游地行动。这是 cyclin D3 玩的第一示范在 B 房间开发的 GC 阶段的一个重要、唯一的角色。 | Jonathan U Peled J Jessica Yu Jeganathan Venkatesh Enguang Bi B Belinda Ding Melissa Krupski-Downs Rita Shaknovich Piotr Sicinski Betty Diamond Matthew D Scharff B Hilda Ye | 2010 | Cell Research2010,20,6: | 3 |
| 4 | 深部地球条件下铁和氧元素的化学变化显示文摘氧与铁分别为地球上丰度最高的元素与占地球质量最大的元素,它们所形成的化合物遍布我们的星球,具有-2价的氧控制着地球的氧逸度,随着深度的增加氧逸度逐渐降低,与铁所形成的氧化物中铁的价态也逐渐降低,O/Fe逐渐增加,近些年来科学家们发现深部地球条件下铁的一系列氧化物,其中具有高压黄铁矿结构的铁超氧化物FeO2具有最高的O/Fe,并且它还能够携带一定量的H(表示为Py-FeO2H,, x=0-1)。 | 夏群科 Liu J Hu Q Y Bi W L Yang L X Xiao Y M Chow P Meng Y.Prakapenka V B Mao H K Mao W L | 2019 | 矿物岩石地球化学通报2019,38,3: | 2 |
| 5 | Interactional justice: Communication criteria of fairness显示文摘 | BIES R J MOAG J S | 1986 | Reaseach on negotiation in organizations1986,1,: | 1 |
| 6 | Rational syntheses, characterization, crystal structure, and replacement reactions of coordinated water molecules of ^10- (M =Cd, Co, Cu, Fe, Mn, Ni or Zn) 显示文摘 | Bi L H Huang R D Peng J | 2001 | J Chem Soc Dalton Trans2001,,: | 1 |
| 7 | Interactional Fairness Judgements:The Influence of Causal Accounts显示文摘 | SHAPIRO D L | 1987 | Social Justice Research1987,,1: | 1 |
| 8 | The recent progress of solvent sublation显示文摘 | Bi P Y Dong H R Dong J | 2010 | J Chromatogr A2010,1217,: | 1 |
| 9 | Central neuropathogenesis of vesicular stomatitis virus infection of immunodeficient mice 显示文摘 | Huneycutt B S Bi Z Aoki C J | 1993 | Journal of Virology1993,67,11: | 1 |
| 10 | Dimcnsionality reduction via sparse support vector machines 显示文摘 | Bi J Bennett K P Embreehts M | 2003 | Journal of Machine Learn- ing Research2003,3,1: | 1 |
| 11 | Rapid preparation of Bi 2 WO 6 photocatalyst with nanosheet morphology via microwave-assisted sol-vothermal synthesis显示文摘 | Wu L Bi J H Li Z H | 2008 | Catalysis Today2008,131,: | 1 |
| 12 | Robust identification of first-order plus dead- time model from step response 显示文摘 | Bi Q Cai W J | 1999 | Control Engineering Practice1999,6,7: | 1 |
| 13 | Assignment of the human reticu- Ion 4 gene (RTN4) to chromosome 2p14-->2p13 by radiation hybrid mapping 显示文摘 | YANG J YU L BI AD | 2000 | Cytogenet Cell Genet2000,88,12: | 1 |
| 14 | How Employees Respond to Interpersonal Offense: The Effects of Blame Attribution, Offender Status, and Victim Status on Revenge and Reconciliation in the Workplace显示文摘 | AQUINO K TRIPP T M BIES R J | 2001 | Journal of Applied Psychology2001,86,1: | 1 |
| 15 | Source apportionment of PM10 in six cities of northern China 显示文摘 | Bi X H Feng Y C Wu J H | 2007 | Atmospheric Environment2007,41,5: | 1 |
| 16 | Study on China's Food Security Status 显示文摘 | Nie F Y Bi J Y Zhang X B | 2010 | Agriculture and Agricultural Science Procedia2010,,1: | 1 |
| 17 | Status of cardiovascular health in Chinese adults 显示文摘 | Bi Y Jiang Y He J | 2015 | J Am Coll Cardiol2015,65,10: | 1 |
| 18 | High- Performance Liquid for the Determination and Pharmacokinetic Study of Mangiferin in Plasma of Rats Having Taken the Traditional Chinese Medicinal Preparation Zi-Shen Pill 显示文摘 | Dai R H Gao J Bi K Chromatographic Method S | 2004 | Joumal of Chromatographic Science2004,42,1: | 1 |
| 19 | Role for sterol regulatory element bin- ding protein - 1 c activation in mediating skeletal muscle insulin re- sistance via repression of rat insulin receptor substrate - 1 tran- scription显示文摘 | Bi Y Wu W Shi J | 2014 | Diabetologia2014,57,3: | 1 |
| 20 | Study on density, hardness, and moisture uptake of ton'efied wood pellets显示文摘 | Peng J H Bi H T Lira C J | 2013 | Energy & Fuels2013,27,2: | 1 |