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| 1 | 外源茉莉酸甲酯诱导茶树鲜叶及其采后乌龙茶加工关键工序中七种脂溶性色素变化显示文摘【目的】系统开展外源茉莉酸甲酯诱导以及“做青”阶段机械损伤双重胁迫下茶叶中脂溶性色素的动态变化研究,阐明外源茉莉酸甲酯诱导后茶树鲜叶制得乌龙茶中类胡萝卜素含量提高的机理,为合理利用外源诱导提升鲜叶品质和加工制得的乌龙茶品质提供科学依据。【方法】0.25%外源茉莉酸甲酯喷施3-4年生盆栽‘金萱’茶树叶面,至均匀挂滴,对不同诱导时间(0、12、24、48和168 h)的鲜叶及利用该鲜叶制作得到的乌龙茶样品进行基于UPLC-QToFMS的靶标代谢组学分析,解析叶黄素、β-胡萝卜素、新叶黄素、玉米黄质、α-胡萝卜素和叶绿素a/b的含量变化;同时对乌龙茶制作的关键工序“做青”以及杀青后揉捻阶段样品中这些脂溶性色素的动态变化进行监测分析。同时,开展不同诱导时间采摘的鲜叶加工得到成品乌龙茶的香气感官评价。【结果】靶标代谢组学分析结果表明,叶黄素是‘金萱’鲜叶中含量最高的类胡萝卜素,其含量达到(405.06±17.71)μg·g^(-1),在成品乌龙茶中含量显著下降,仅为(277.36±32.72)μg·g^(-1)。外源茉莉酸甲酯诱导后,脂溶性色素含量变化显著,诱导48 h内,茶树鲜叶中叶绿素a含量较对照鲜叶中(0 h)增加,随后显著下降;叶绿素b在诱导后的鲜叶中始终处于减少的趋势。叶黄素在12 h样品中含量显著下降,在24 h的样品中其含量均较对照升高;β-胡萝卜素在诱导后的鲜叶中含量始终低于对照,其中12 h的样品中含量仅为116.36μg·g^(-1),减少34.55%。利用茉莉酸甲酯诱导后的鲜叶(12、24和48 h)制作得到的成品茶样品中叶绿素a含量较对照均显著下降;叶黄素含量显著升高,其中48 h的样品中含量最高,达到377.82μg·g^(-1)。“做青”过程中(W1-W3),对7种脂溶性色素含量变化进行相应的热图分析,与对照相比,叶绿素a含量均显著下降,而叶绿素b含量则在12 h样品中显著升高。5种类胡萝卜素的含量动态变化则更加复杂,含量最高的叶黄素在12 h样品中含量显著下降,随后在24和48 h的样品中含量略有升高;β-胡萝卜素的含量始终低于对照。此外,玉米黄质和α-胡萝卜素在诱导12 h以后的样品中始终维持非常高的含量。揉捻阶段的样品,7种脂溶性色素也呈现出显著变化,除了β-胡萝卜素含量较对照样品减少,叶黄素、新叶黄素、玉米黄质以及α-胡萝卜素的含量在诱导12 h后的样品中均比对照高。茉莉酸甲酯诱导的样品中,叶绿素a的含量降低,叶绿素b的含量在诱导12 h后增加。此外,茶叶感官审评结果表明,外源茉莉酸甲酯诱导后茶树鲜叶加工得到的乌龙茶香气品质显著提升,具有持久浓郁的花香,然而其叶底明亮度和柔软性较未处理的对照样品有所下降。【结论】外源茉莉酸甲酯诱导茶树鲜叶,24 h内可以显著提高鲜叶及成品茶中的类胡萝卜素含量,诱导12 h后鲜叶加工得到的成品茶香气品质显著提高,具有浓郁的花香。外源诱导与“做青”机械损伤双重胁迫激发茶鲜叶中脂溶性色素的差异积累。 | 施江 王佳童 彭群华 吕海鹏 BALDERMANN susanne 林智 | 2021 | 中国农业科学2021，54，18： | 2 |
| 2 | Isolation and identification of compounds from the ethanolic extract of flowers of the tea ( Camellia sinensis ) plant and their contribution to the antioxidant capacity显示文摘 | Ziyin Yang Youying Tu Susanne Baldermann Fang Dong Yi Xu Naoharu Watanabe | 2009 | LWT - Food Science and Technology2009，，8： | 2 |
| 3 | Integrated proteomic and metabolomic analyses reveal the importance of aroma precursor accumulation and storage in methyl jasmonate-primed tea leaves显示文摘In response to preharvest priming with exogenous methyl jasmonate(MeJA),tea plants adjust their physiological behavior at the molecular level.The whole-organism reconfiguration of aroma formation from the precursor to storage is poorly understood.In this study,we performed iTRAQ proteomic analysis and identified 337,246,and 413 differentially expressed proteins in tea leaves primed with MeJA for 12 h,24h,and 48 h,respectively.Furthermore,a total of 266 nonvolatile and 100 volatile differential metabolites were identified by utilizing MS-based metabolomics.A novel approach that incorporated the integration of extended self-organizing map-based dimensionality was applied.The vivid time-scale changes tracing physiological responses in MeJA-primed tea leaves are marked in these maps.Jasmonates responded quickly to the activation of the jasmonic acid pathway in tea leaves,while hydroxyl and glycosyl jasmonates were biosynthesized simultaneously on a massive scale to compensate for the exhausted defense.The levels ofα-linolenic acid,geranyl diphosphate,farnesyl diphosphate,geranylgeranyl diphosphate,and phenylalanine,which are crucial aroma precursors,were found to be significantly changed in MeJA-primed tea leaves.Green leaf volatiles,volatile terpenoids,and volatile phenylpropanoids/benzenoids were spontaneously biosynthesized from responding precursors and subsequently converted to their corresponding glycosidic forms,which can be stably stored in tea leaves.This study elucidated the physiological response of tea leaves primed with exogenous methyl jasmonate and revealed the molecular basis of source and sink changes on tea aroma biosynthesis and catabolism in response to exogenous stimuli.The results significantly enhance our comprehensive understanding of tea plant responses to exogenous treatment and will lead to the development of promising biotechnologies to improve fresh tea leaf quality. | Jiang Shi Jiatong Wang Haipeng Lv Qunhua Peng Monika Schreiner Susanne Baldermann Zhi Lin | 2021 | Horticulture Research2021，8，1： | 2 |
| 4 | Changes of hepatic morphology during parenteral nutrition with lipid emulsions containing LCT or MCT/LCT quantified by ultrasound 显示文摘 | BALDERMANN H WICKLMAYR M RETT K | 1996 | Journal of Parenteral and Enteral Nutrition1996，15，6： | 1 |
| 5 | solation of all-trans lycopene by high-speed counter-current chromatography using a temperature-controlled solvent system显示文摘 | SUSANNE BALDERMANN KATHARINA ROPETER | 2008 | Journal of Chromatography A2008，1192，3： | 1 |
| 6 | Recent studies of the volatile compounds in tea 显示文摘 | Yang Z Baldermann S Watanabe N | 2013 | Food Research International2013，53，2： | 1 |
| 7 | Recent studies of the volatile compounds in tea显示文摘 | Yang Z Y Baldermann S Watanabe N | 2013 | Food Research International2013，53，： | 1 |
| 8 | Platelet FegammaRIIA expression is associated with the alpha2 integrin C807T gene polymorphism in type 2 diabetes显示文摘 | Calverley DC Baldermann LV Moran K | 2006 | Platelets2006，17，2： | 1 |
| 9 | Recent studies of the volatile compounds in tea显示文摘 | Ziyin Yang Susanne Baldermann Naoharu Watanabe | 2013 | Food Research International2013，，： | 1 |
| 10 | Enzymatic carotenoid degradation and aroma formation in nectarines(Prunus persica)显示文摘 | Baldermann S M Naim P Fleischmann | 2005 | Food Research International2005，38，83： | 1 |
| 11 | Recent studios of the volatile compounds in tea显示文摘 | YANG Z BALDERMANN S WATANABE N | 2013 | Food Research Interna- tional2013，53，2： | 1 |
| 12 | Isolation of all-trans lycopene by high-speed countercurrent chromatography using a temperature-controlled solvent system显示文摘 | BALDERMANN S ROPETER K KOHLER N | 2008 | Journal of Chromatography A2008，1192，1： | 1 |
| 13 | Metabolism of Carotenoid Degradation in leaves of Camellia sinensis-Functional and biochemical modifications显示文摘 | Felfe C Schemainda M Baldermann S Watanabe N Fleischmann P | | 0，，06： | 1 |
| 14 | Isolation and identification of compounds from the ethanolic extract of flowers of the tea (Camellia sinensis) plant and their contribution to the antioxidant capacity显示文摘 | YANG Z TU Y BALDERMANN S | 2009 | International Journal of Food Science and Technology2009，42，8： | 1 |
| 15 | Novel green technology for wastewater treatment:Geo-material/geopolymer applications for heavy metal removal from aquatic media显示文摘The aim of this paper is to show the concise chemico-physical adsorbent performance of water purification systems utilizing geo-(e.g., allophane, clinoptilolite, and smectite) and bio-polymer materials(e.g.,chitosan or cellulose nanocomposite materials) and to propose an optimal ground-water remediation technique. The performance of geo-materials is evaluated based on the individual sorption and immobilization capacities for various priority substances and pollutants(e.g., lead, zinc, cadmium, copper,arsenic, and others), their availability, and cost-efficient use. A systematic assessment of the sorption potential of geo-materials in comparison to other available sorbents used for the removal of harmful aqueous metal ions is made through a literature review. This paper introduces novel sustainable technologies based on natural and tailored silicate-polymerized substances(geo-materials and geopolymers),and highlights their applicability in the treatment of water and solid matter contaminated by heavy metal ions. The advantages of geo-materials and geopolymers over other commercially available sorbents used for heavy metal ion removal from solution are presented through a literature review. Benefits and current challenges of geo-materials and geopolymers applications in water processing technologies and in environmental remediation are discussed, with recognition of their performance, individual sorption and immobilization capacities, availability, and cost-efficient use. The applications described here comprise:(i) the removal of heavy metal ions from contaminated water using in-situ remediation strategies;(ii) heavy metal ion immobilization through co-precipitation with silicate binders in underground stabilization and waste solidification scenarios;and(iii) a proposal for a new geo-material/geopolymer-based solidification and stabilization technology for efficient, sustainable, and simultaneous treatment of soil/sediment and groundwater at environmental hotspots. Clay-substituted geopolymers, smectite, and zeolites are distinguished by their superb sorption and immobilization capacities for heavy metal ions, while biosorbents can play an important role in the removal of metals, metalloids(e.g., arsenic), and other contaminants. More research on individual removal mechanisms of heavy metals will provide new clues on the development of remediation strategies in advanced scientific and field applications, and on atomic-to-micron scale processes occurring at the solid-liquid interface. | Nenad Grba Andre Baldermann Martin Dietzel | 2023 | International Journal of Sediment Research2023，38，1： | 1 |
| 16 | The rate and mechanism of deep-sea glauconite formation at the Ivory Coat- Ghana marginal ridge显示文摘 | Baldermann A Wart L N Grathoff G H | 2013 | Clay and Clay Minerals2013，61，： | 1 |
| 17 | Recent studies of the volatile compounds in tea显示文摘 | Yang Z Baldermann S Watanabe N | 2013 | Food Research International2013，53，2： | 1 |
| 18 | Enzymatic carotenoid degradation and aroma formation in nectarines (Prunus persica) 显示文摘 | Baldermann S Naim M Fleischmann P | 2005 | Food research international2005，38，8： | 1 |
| 19 | Recent studies of the volatile compounds in tea 显示文摘 | Yang Z Baldermann S Watanabe N | 2013 | Food Research International2013，53，2： | 1 |
| 20 | Enzymatic carotenoid degradation and aroma formation in nectarines ( Prunus persica) 显示文摘 | Baldermann S Naim M Fleischmann P | 2005 | Food Research Int2005，38，89： | 1 |
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