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| 1 | Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties显示文摘In rice, amylose content(AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-associated 9(Cas_9) to introduce a loss-of-function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties. | Jinshan Zhang Hui Zhang Jose Ramón Botella Jian-Kang Zhu | 2018 | Journal of Integrative Plant Biology2018，60，5： | 49 |
| 2 | Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System显示文摘 | Mugui Wang Yuming Lu Jose Ramon Botella Yanfei Mao Kai Hua Jian-kang Zhu | 2017 | Molecular Plant2017，10，7： | 34 |
| 3 | Perspectives on the Application of Genome-Editing Technologies in Crop Breeding显示文摘Most conventional and modern crop-improvement methods exploit natural or artificially induced genetic variations and require laborious characterization of the progenies of multiple generations derived from time-consuming genetic crosses.Genome-editing systems,in contrast,provide the means to rapidly modify genomes in a precise and predictable way,making it possible to introduce improvements directly into elite varieties.Here,we describe the range of applications available to agricultural researchers using existing genome-editing tools.In addition to providing examples of genome-editing applications in crop breeding,we discuss the technical and social challenges faced by breeders using genome-editing tools for crop improvement. | Kai Hua Jinshan Zhang Jose Ramon Botella Changle Ma Fanjiang Kong Baohui Liu Jian-Kang Zhu | 2019 | Molecular Plant2019，12，8： | 19 |
| 4 | A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana显示文摘The newly developed CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)/Cas(CRISPR-associated) system has emerged as an efficient tool for genome-editing, providing an alternative to classical mutagenesis and transgenic methods to study gene function and improve crop traits. CRISPR/Cas facilitates targeted gene editing through RNA-guided DNA cleavage followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. Here we describe a detailed procedure for our previously developed and highly efficient CRISPR/Cas9 method that allows the generation of heritable-targeted gene mutations andcorrections in Arabidopsis. This protocol describes the strategies and steps for the selection of targets, design of single-guide RNA(sg RNA), vector construction and analysis of transgenic lines. We also offer a method to target two loci simultaneously using vectors containing two different sg RNAs. The principles described in this protocol can be applied to other plant species to generate stably inherited DNA modifications. | Wenshan Liu Xiaohong Zhu Mingguang Lei Qingyou Xia Jose Ramon Botella Jian-Kang Zhu Yanfei Mao | 2015 | Science Bulletin2015，60，15： | 18 |
| 5 | Gene editing in plants:progress and challenges显示文摘The clustered regularly interspaced short palindromic repeat(CRISPR)-associated protein 9(Cas9)genome editing system is a powerful tool for targeted gene modifications in a wide range of species,including plants.Over the last few years,this system has revolutionized the way scientists perform genetic studies and crop breeding,due to its simplicity,flexibility,consistency and high efficiency.Considerable progress has been made in optimizing CRISPR/Cas9 systems in plants,particularly for targeted gene mutagenesis.However,there are still a number of important challenges ahead,including methods for the efficient delivery of CRISPR and other editing tools to most plants,and more effective strategies for sequence knock-ins and replacements.We provide our viewpoint on the goals,potential concerns and future challenges for the development and application of plant genome editing tools. | Yanfei Mao Jose Ramon Botella Yaoguang Liu Jian-Kang Zhu | 2019 | National Science Review2019，6，3： | 17 |
| 6 | Arabidopsis Squalene Epoxidase 3 (SQE3) Complements SQE1 and Is Important for Embryo Development and Bulk Squalene Epoxidase Activity显示文摘在 mevalonate 小径的 multigenic 家庭的存在为涉及植物甾醇的生合成的小径部件建议分叉的功能的角色。Squalene epoxidases (SQE ) 是这条小径的关键部件,并且 Squalene Epoxidase 1 (SQE1 ) 在这 biosynthetic 步作为基本的酶被识别了。在现在的工作,我们扩大了留下的 SQE 家庭成员的描述,种系发生地在真 SQE 和对 Brassicaceae 独占的象 SQE 一样蛋白质的一个亚科之间解决。真 SQE 家庭成员, Squalene Epoxidase 2 (SQE2 ) 和 Squalene Epoxidase 3 (SQE3 ) 的功能的描述,显示那 SQE3,然而并非 SQE2,在 Arabidopsis 贡献体积 SQE 活动,与积累 squalene 并且显示敏感到 terbinafine 的 sqe3-1 异种。我们遗传上证明 SQE3 似乎在胚胎开发起一个特别地重要的作用。另外, SQE1 和 SQE3 两个都在 endoplasmic 蜂窝胃本地化,并且 SQE3 能机能上地补充 SQE1。因此, SQE1 和 SQE3 似乎是在在 Arabidopsis 的工厂 SQE 活动的提升的二机能上地不相等的冗余的基因。 | Sara Laranjeira Vitor Amorim-Silva Alicia Esteban Monserrat Arro Albert Ferrer Rui Manuel Tavares Miguel Angel Botella Abel Rosado Herlander Azevedo | 2015 | Molecular Plant2015，8，7： | 12 |
| 7 | The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis显示文摘1 变应原是的草莓兄弟主要桦树花粉变应原的一个相当或相同的事物赌了 v 1。当变异的遗传型的白水果,被知道被过敏症影响的个人容忍,缺乏它时,它被 Fragaria x ananassa 的红成熟水果综合。Proteomic 分析显示出 1 是的那个兄弟在与 anthocyanin 颜料小径的酶一起的容忍的白水果的遗传型下面调整。在这研究,我们报导三个兄弟的空间、时间的表示一编码不同 isoforms 的基因,和兄弟的短暂调停 RNAi 的 silencing 一在有 ihpRNA 的红水果的栽培变种 Elsanta 的草莓水果的基因构造。作为兄弟的减少的层次的后果 mRNAs,水果被获得那生产了 anthocyanins 和在上游的代谢物的显著地减少的层次。这效果与本氨基丙酸氨 lyase (FaPAL ) 的平行下面规定一致,到 chalcone synthase (FaCHS ) 的更小的程度,抄本层次也在这些水果发现。在 F 的自然地发生的白水果的遗传型。chiloensis 和 F。vesca,一个抄本铺平的兄弟比红水果的变化的那些高,可能在这些变异的遗传型补偿 FaPAL 和 FaCHS 的低表示层次。结果表明那个兄弟表情直接被连接到 flavonoid 生合成和表演变应原有的兄弟在在草莓的颜料形成的必要生物功能水果。 | Cristina Munoz Thomas Hoffmann Nieves Medina Escobar Felix Ludemann Miguel A. Botella Victoriano Valpuesta Wilfried Schwab | 2010 | Molecular Plant2010，3，1： | 6 |
| 8 | Multicell power allocation method based on game theory for inter-cell interference coordination显示文摘As a new technology, coordinated multipoint (CoMP) transmission is included in LTE-Advanced study item. Moreover, the network architecture in LTE-Advanced system is modified to take into account coordinated transmission. Under this background, a novel power allocation game model is established to mitigate inter-cell interference with cellular coordination. In the light of cellular cooperation relationship and centralized control in eNodeB, the power allocation in each served antenna unit aims to make signal to interference plus noise ratio (SINR) balanced among inter-cells. Through the proposed power allocation game algorithm, the users' SINR can reach the Nash equilibrium, making it feasible to reduce the co-frequency interference by decreasing the transmitted power. Numerical results show that the proposed power allocation algorithm improves the throughput both in cell-center and cell-edge. Moreover, the blocking rate in cell-edge is reduced too. | ZHANG Hui XU XiaoDong LI JingYa TAO XiaoFeng ZHANG Ping SVENSSON Tommy BOTELLA Carmen | 2009 | Science in China(Series F)2009，52，12： | 5 |
| 9 | Genome editing for plant research and crop improvement显示文摘The advent of clustered regularly interspaced short palindromic repeat(CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel(insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control. | Xiangqiang Zhan Yuming Lu Jian-Kang Zhu Jose Ramon Botella | 2021 | Journal of Integrative Plant Biology2021，63，1： | 5 |
| 10 | Performance of power control in inter-cell interference coordination for frequency reuse显示文摘To mitigate inter-cell interference in 3G evolution systems,a novel inter-cell interference coordination scheme called soft fractional frequency reuse is proposed in this article,which enables to improve the data rate in cell-edge.On this basis,an inter-cell power control is presented for the inter-cell interference coordination,and the inter-cell balanced signal to interference plus noise ratio(SINR) among users is established for power allocation,which enables mitigation of inter-cell interference.Especially,the power control is based on a novel exponential kernel equation at higher convergence speed than the traditional arithmetic kernel equations.Numerical results show that the proposed scheme improves the throughput and reduces the blocking rate compared to the existing power control algorithms. | ZHANG Hui XU Xiao-dong LI Jing-ya TAO Xiao-feng Tommy Svensson Carmen Botella | 2010 | The Journal of China Universities of Posts and Telecommunications2010，17，1： | 2 |
| 11 | Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings显示文摘 | Borsani O Valpuesta V Botella M | 2001 | Journal of Plant Physiology2001，126，： | 1 |
| 12 | Selecive oxidation of propane to acrylic acid on MoVNbTe mixed oxides catalysts prepared by hydrothemal synthesis 显示文摘 | Botella P Solsona B Martinez-Arias A | 2001 | Catalysis Letters2001，74，34： | 1 |
| 13 | The use of VR in the treatment of panic disorders and agoraphobia显示文摘 | Botella C Villa H García Palacios A | | 0，，： | 1 |
| 14 | Selective oxidative dehydrogenation of ethane over MoVSbO mixed oxide catalysts显示文摘 | Botella P Dejoz A Nieto J M L Concepción P Vázquez M I | | 0，，： | 1 |
| 15 | The preparation, characterization and catalytic behaviour of MoVTeNbO catalysts preparedby hydrothermal synthesis显示文摘 | Botella P Lopez Nieto J M Solsona B | 2002 | Journal of Catalysis2002，209，2： | 1 |
| 16 | Adiponectin and resistin in PCOS: A clinical, biochemical and molecular genetic study显示文摘 | Escobar M H F Villuendas G Botella C J I | 2006 | Hum Reprod2006，21，： | 1 |
| 17 | Task and location- switching effects on visual attention显示文摘 | Juola J F Botella J Palacios A | 2004 | Perception Psychophysics2004，66，8： | 1 |
| 18 | Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Spl and KLF6: their potential role in the response to vascular injury显示文摘 | Botella LM Sanchez-Elsner T Sanz-Rodriguez F | 2002 | Blood2002，100，12： | 1 |
| 19 | Influence of chemical composition on the pitting corrosion resistance of non-standard low-Ni high-Mn–N duplex stainless steels显示文摘 | R. Merello F.J. Botana J. Botella M.V. Matres M. Marcos | 2002 | Corrosion Science2002，，5： | 1 |
| 20 | Oxidative dehydrogenation of ethane on Te-containing MoVNbO catalysts显示文摘 | L(O)PEZ NIETO J M BOTELLA P CONCEPCI(O)N P | 2004 | Catal Today2004，9192，： | 1 |
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