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| 1 | Extraction and RP-HPLC determination of taxol in rat plasma, cell culture and quality control samples显示文摘A rapid,sensitive,selective and validated reverse phase high-performance liquid chromatography(RP-HPLC)method for the estimation of paclitaxel in micro-sample of rat plasma and in culture of cancer cells was performed in this study.The mobile phase consisted of an optimized mixture of methanol:water:trifluroacetic acid(80:20:0.1,v/v/v).Column elution at a flow rate of 1 mL/minute with UV detection at 225 nm at room temperature was used.The RP-HPLC method was successfully applied for the determination of paclitaxel in plasma samples and in culture of cancer cells with nano-quantity of estimation.The validation studies were performed in accordance with the International Conference on Harmonization(ICH)guidelines.The intra-and inter-day precision showed that the coefficients of variation ranged from 1.07%to 4.27%at different levels of concentrations.To the best of our knowledge,this study also reported for the first time the optimization of different solvents for effective extraction of paclitaxel wherein tert.-butyl methyl ether(TBME):diethyl ether(DEE)in 50:50 v/v composition was found most efficient with extraction efficiency ranging between 77.99%and 91.74%and between 76.14 and 93.66%in the plasma and cell culture,respectively.This proposed method was successfully applied to study the pharmacokinetics of paclitaxel and the influence of verapamil and all-trans retinoic acid(atRA)on paclitaxel pharmacokinetics in rat models.This proposed method might emerge as a valuable aid in the laboratory monitoring of paclitaxel in a variety of in vitro as well as in vivo scenarios. | Rakesh Kumar Tekade Antony D'Emanuele Abdelbary Elhissi Ashish Agrawal Anurekha Jain Basel Tawfiq Arafat Narendra Kumar Jain | 2013 | The Journal of Biomedical Research2013，27，5： | 5 |
| 2 | Treatment with interferon-a2b and ribavirin improves outcome in MERS-CoV-infected rhesus macaques 显示文摘 | Falzarano D de Wit E Rasmussen AL Feldmann F Oku- mura A Scott DP Brining D Bushmaker T Martellaro C Baseler L Benecke AG Katze MG Munster VJ Feldma- nn H | 2013 | Nat Med2013，19，10： | 1 |
| 3 | Association of Piebaldism, multiple cafe-au-lait maeules, and intertriginous freckling: clinical evidence of a common pathway between KIT and sprouty-related, ena/vasodilator- stimulated phosphoprotein homology - 1 domain containing protein 1 ( SPRED1 ) 显示文摘 | Chiu YE Dugan S Basel D | 2013 | Pediatr Dermato12013，30，3： | 1 |
| 4 | Split hand foot malformation is associated with a reduced level of Dactylin gene expression显示文摘 | Basel D DePaepe A Kilpatrick MW | 2003 | Clin Genet2003，64，： | 1 |
| 5 | miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart显示文摘 | Baseler WA Thapa D Jagannathan R | 2012 | Am J Physiol Cell Physiol2012，303，12： | 1 |
| 6 | miR-141 as a regulatorof the mitochondrial phosphate carrier (Slc25a3) in the type 1diabetic heart显示文摘 | Baseler WA Thapa D Jagannathan R | 2012 | Am J Physiol Cell Physiol2012，303，12： | 1 |
| 7 | Direct and indirect induction by 1,25-dihydroxyvitamin D3 of the NOD2/CARD15-defensin beta2 innate immune path- way defective in Crohn disease 显示文摘 | TIAN-TIAN W BASEL D DAVID L | 2010 | Journal of Biological Chemistry2010，285，4： | 1 |
| 8 | Mir- 141 as a regulator of the mitochondrial phosphate carrier (slc25a3) in the type 1 diabetic heart显示文摘 | BASELER WA THAPA D JAGANNATHAN R | 2012 | Am J Physiol Cell Physiol2012，303，12： | 1 |
| 9 | Osteogenesis imperfecta: recent findings shed new light on this once well understood condition显示文摘 | Basel D Steiner RD | 2009 | Genet Med2009，11，： | 1 |
| 10 | Cyclin AI shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV 16 显示文摘 | Weiss D Koopmann M Basel T | 2012 | BMC Cancer2012，12，： | 1 |
| 11 | Distal limb malformations: underlying mechanisms and clinical associa tions 显示文摘 | Sifakis S Basel D Ianakiev P Kilpatriek M Tsipouras P | 2001 | Clin Genet2001，60，3： | 1 |
| 12 | HIV-1 and T cell dynamics after interruption of highly active antiretroviral therapy (HAA T) in patients with a history of sustained viral suppression 显示文摘 | Davey RT Bhat N Yoder C Chun TW Metcalf JA Dewar R Natarajan V Lempicki RA Adelsberger JW Miller KD Kovacs JA Polis MA Walker RE Falloon J Masur H Gee D Baseler M Dimitrov DS Fauci AS Lane HC | 1999 | Proc Natl Acad Sci USA1999，96，15： | 1 |
| 13 | Treatment with interferon-al- pha2b and ribavirin improves outcome in mers-cov-infec- ted rhesus macaques显示文摘 | Falzarano D de Wit E Rasmussen A L Feldmann F Okumura A Scott D P Brining D Bushmaker T Mar- tellaro C Baseler L Benecke A G Katze M G Mun ster V J Feldmann H | 2013 | Nat Med2013，19，10： | 1 |
| 14 | Osteogenesis imperfecta:recent findings shed new light on this once well-understood condition显示文摘 | Basel D Steiner RD | 2009 | Genet Med2009，11，6： | 1 |
| 15 | Colton a bio2 sensor based on magnetoresistance technology显示文摘 | BASEL T D R LEE G U NATESAN M | 1998 | Biosensors & Bioelectronics1998，13，73： | 1 |
| 16 | The expanding panorama of split hand foot malformation 显示文摘 | Basel D Kilpatriek MW Tsipouras P | 2006 | Am J Med Genet Part A2006，140，13： | 1 |
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