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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Sensitivity analysis of parameters and contrasting performance of ground filtering algorithms with UAV photogrammetry-based and LiDAR point clouds显示文摘Most ground filtering algorithms are primarily designed for airborne LiDAR point cloud processing and their successful use in identifying ground points from photogrammetric point clouds remains questionable.We compared six ground filtering algorithms implemented in Metashape,ArcGIS,CloudCompare,LAStools,and PDAL.We used UAV photogrammetry-based(acquired under leaf-off conditions)and airborne LiDAR(leaf-on)point clouds of the same area to:(i)compare accuracy of generated DTMs;(ii)evaluate the effect of vegetation density and terrain slope on filtering accuracy;and(iii)assess which algorithm parameters have the greatest effect on the filtering accuracy.Our results show that the performance of filtering algorithms was affected by the point cloud type,terrain slope and vegetation cover.The results were generally better for LiDAR(RMSE 0.13–0.19 m)than for photogrammetric(RMSE 0.19–0.23 m)point clouds.The behavior in varying vegetation and terrain conditions was consistent for LiDAR point clouds.However,when applied on photogrammetric point clouds,the algorithms’behavior was inconsistent,especially in areas of steep slope(except for the Progressive Triangulated Irregular Network in LAStools).Parameters related to the selection of the initial minimum elevation ground points were the most influential in all algorithms and point clouds. | Petr Klápště Michal Fogl Vojtěch Barták Kateřina Gdulová Rudolf Urban Vítězslav Moudrý | 2020 | International Journal of Digital Earth2020，13，12： | 2 |
| 3 | Coupled package-device nxxtding for microelectromechanical systems 显示文摘 | Bart S F Zhang Su-san Rablnovich V L | 2000 | Microelectronics and Rdiability2000，40，： | 1 |
| 4 | The PolyMAX frequency domain method: a new standard for modal parameter estimation 显示文摘 | BART P HERMAN V D A PATRICK G | 2004 | Shock and Vibration2004，，11： | 1 |
| 5 | Determinants and consequences of trust in e-business:a large scale empirical study显示文摘 | SULTAN F URBAN G L SHANKAR V BART I | 2002 | Sloan school of Management MIT Working Paper2002，2002，： | 1 |
| 6 | Amplification in service supply chains: an exploratory case study from the telecom industry 显示文摘 | Henk Akkermans Bart V | 2003 | Production And Operations Management2003，12，2： | 1 |
| 7 | Cryosurgery in aggressive,benign,and low-grade malignant bone tumours显示文摘 | RenéV Bart S Hermanvan B | 2005 | Lancet Oncol2005，6，： | 1 |
| 8 | The PloyMAX frequency domain method:a new standard for modal parameter estimation显示文摘 | Bart P Herman V D A Patrick G | 2004 | Shock and Vibration2004，，11： | 1 |
| 9 | A multilinear singular value decomposition显示文摘 | Lieven D Bart D Joos V | 2000 | SIAM J Matrix Anal Appl2000，21，4： | 1 |
| 10 | Improved ruthenium catalysts for the modifed friedlaender quinoline synthesis显示文摘 | Hans V M Nele L Bart A | 2007 | New J Chem2007，31，： | 1 |
| 11 | Continuous-time frequency domain subspace system identification显示文摘 | Peter V O Bart D M | 1996 | Signal Processing1996，52，2： | 1 |
| 12 | A review of pressure-driven membrane processes in wastewater treatment and drinking water production 显示文摘 | Bart V D B Carlo V Tim V G | 2003 | Environmental Progress2003，22，1： | 1 |
| 13 | Application of RANS and LES field simulations to predict the critical ventilation velocity in longitudinally ventilated horizontal tunnels 显示文摘 | KARIM V BART M | 2008 | Fire Safety Journal2008，43，： | 1 |
| 14 | Bayesian Network Classifiers for Identifying the Slope of the Customer Lifecycle of Long-life Customers显示文摘 | Bart B Geert V | 2004 | European Journal of Operational Research2004，，156： | 1 |
| 15 | Are the Drivers and Role of Online Trust the Same for All Web Sites and Consumers A Large Scale Exploratory Empirical Study显示文摘 | Bart Y Shandar V Sultan F | 2005 | Journal of Marketing2005，69，： | 1 |
| 16 | Molecular cloning and nucleotide sequence of hog cholera envelope glycoprotein EI 显示文摘 | Moonmann R J M Warmendarm P A M Bart V M | 1990 | Virology1990，177，： | 1 |
| 17 | Spiking the MERS-coronavirus receptor显示文摘 | Berend Jan Bosch V Stalin Raj Bart L Haagmans | 2013 | Cell Research2013，23，9： | 1 |
| 18 | Prediction of Dose Escalation for Rheumatoid Arthritis Patients under Infliximab Treatment显示文摘 | Stijn V L Bert V C Jeroen M Bart W | 2006 | Engineering Applications of Artificial Intelligence2006，19，7： | 1 |
| 19 | PI3K in lymphocyte development, differentation and activation显示文摘 | Klaus O Bart V | 2003 | Nature reviews immulogy2003，3，： | 1 |
| 20 | Antigen receptor signalling: a distinctive role for the p110δ isoform of PI3K显示文摘 | Klaus O Khaled A Bart V | 2007 | Trends in Immunology2007，28，2： | 1 |
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