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| 1 | 犬体内细粒棘球绦虫和多房棘球绦虫的混合感染显示文摘目的 证实家犬体内是否存在细粒棘球绦虫和多房棘球绦虫混合感染。方法 从新疆和静县巴音布鲁克草原现场收购的30条牧羊犬,经麻醉后处死解剖,在1只雌性牧羊犬小肠内发现棘球绦虫成虫1万条以上,经显微镜观察,疑为细粒棘球绦虫和多房棘球绦虫混合感染。用Eglf/r和EM-15/17EM引物分别对两种棘球绦虫的线粒体DNA特异目的片段进行序列分析鉴定。结果 形态学观察:细粒棘球绦虫孕节长大,生殖孔偏后,位于节片一侧中部。子宫有不规则的分支和侧突(侧囊),内含虫卵200~800个。多房棘球绦虫较短小,4~5体节。孕节中子宫呈简单的囊状,无侧囊。生殖孔开口于侧缘的前半部。线粒体12S RNA序列鉴定,扩增样本DNA经同源序列比较发现,与细粒棘球绦虫G1型具有相同的序列;扩增样本与多房棘球绦虫具有相同的序列。分别确定为细粒棘球绦虫和多房棘球绦虫两种虫种。结论 首次证实家犬体内存在细粒棘球绦虫和多房棘球绦虫的混合感染。 | 温浩 张亚楼 Jean-Mathieu BART Giraudoux P Vuitton DA 马旭东 邹林樾 苗玉清 Craig PS | 2006 | 中国寄生虫学与寄生虫病杂志2006，24，1： | 18 |
| 2 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 3 | Near-infrared fluorescence guided esophageal reconstructive surgery: A systematic review显示文摘BACKGROUND After an esophagectomy, the stomach is most commonly used to restore continuity of the upper gastrointestinal tract. These esophago-gastric anastomoses are prone to serious complications such as leakage associated with high morbidity and mortality. Graft perfusion is considered to be an important predictor for anastomotic integrity. Based on the current literature we believe Indocyanine green fluorescence angiography(ICGA) is an easy assessment tool for gastric tube(GT) perfusion, and it might predict anastomotic leakage(AL).AIM To evaluate feasibility and effectiveness of ICGA in GT perfusion assessment and as a predictor of AL.METHODS This study was designed according to the PRISMA guidelines and registered in the PROSPERO database. PubMed and EMBASE were independently searched by 2 reviewers for studies presenting data on intraoperative ICGA GT perfusion assessment during esophago-gastric reconstruction after esophagectomy.Relevant outcomes such as feasibility, complications, intraoperative surgical changes based on ICGA findings, quantification attempts, anatomical data and the impact of ICGA on postoperative anastomotic complications, were collected by 2 independent researchers. The quality of the included articles was assessed based on the Methodological Index for Non-Randomized Studies. The 19 included studies presented data on 1192 esophagectomy patients, in 758 patients ICGA was used perioperative to guide esophageal reconstruction.RESULTS The 19 included studies for qualitative analyses all described ICGA as a safe and easy method to evaluate gastric graft perfusion. AL occurred in 13.8% of the entire cohort, 10% in the ICG guided group and 20.6% in the control group(P <0.001). When poorly perfused cases are excluded from the analyses, the difference in AL was even larger(AL well-perfused group 6.3% vs control group 20.5%, P <0.001). The AL rate in the group with an altered surgical plan based on the ICG image was 6.5%, similar to the well perfused group(6.3%) and significantly less than the poorly perfused group(47.8%)(P < 0.001), suggesting that the technique is able to identify and alter a potential bad outcome.CONCLUSION ICGA is a safe, feasible and promising method for perfusion assessment. The lower AL rate in the well perfused group suggest that a good fluorescent signal predicts a good outcome. | Elke Van Daele Yves Van Nieuwenhove Wim Ceelen Christian Vanhove Bart P Braeckman Anne Hoorens Jurgen Van Limmen Oswald Varin Dirk Van de Putte Wouter Willaert Piet Pattyn | 2019 | World Journal of Gastrointestinal Oncology2019，11，3： | 3 |
| 4 | Sensitivity analysis of parameters and contrasting performance of ground filtering algorithms with UAV photogrammetry-based and LiDAR point clouds显示文摘Most ground filtering algorithms are primarily designed for airborne LiDAR point cloud processing and their successful use in identifying ground points from photogrammetric point clouds remains questionable.We compared six ground filtering algorithms implemented in Metashape,ArcGIS,CloudCompare,LAStools,and PDAL.We used UAV photogrammetry-based(acquired under leaf-off conditions)and airborne LiDAR(leaf-on)point clouds of the same area to:(i)compare accuracy of generated DTMs;(ii)evaluate the effect of vegetation density and terrain slope on filtering accuracy;and(iii)assess which algorithm parameters have the greatest effect on the filtering accuracy.Our results show that the performance of filtering algorithms was affected by the point cloud type,terrain slope and vegetation cover.The results were generally better for LiDAR(RMSE 0.13–0.19 m)than for photogrammetric(RMSE 0.19–0.23 m)point clouds.The behavior in varying vegetation and terrain conditions was consistent for LiDAR point clouds.However,when applied on photogrammetric point clouds,the algorithms’behavior was inconsistent,especially in areas of steep slope(except for the Progressive Triangulated Irregular Network in LAStools).Parameters related to the selection of the initial minimum elevation ground points were the most influential in all algorithms and point clouds. | Petr Klápště Michal Fogl Vojtěch Barták Kateřina Gdulová Rudolf Urban Vítězslav Moudrý | 2020 | International Journal of Digital Earth2020，13，12： | 2 |
| 5 | Treatment of organic pollutants in coke plant wastewater by the method of ultrasonic irradiation, catalytic oxidation and activated sludge显示文摘 | NING P BART H J JIANG Y J | 2005 | Separation and Purification Technology2005，41，2： | 1 |
| 6 | The PolyMAX frequency domain method: a new standard for modal parameter estimation 显示文摘 | BART P HERMAN V D A PATRICK G | 2004 | Shock and Vibration2004，，11： | 1 |
| 7 | Falling Film Evap- oration with Polymeric Heat Transfer Surfaces 显示文摘 | Christmann Joehen B P Kritz L J Bart H J | 2011 | Desalination2011，，： | 1 |
| 8 | The prognostic value of histology in the assessment of patients with Budd–Chiari syndrome显示文摘 | Thjon J Tang Kenneth P Batts Piet C de Groen Bart van Hoek Elizabeth B Haagsma Wim C.J Hop Harry L.A Janssen | 2001 | Journal of Hepatology2001，，3： | 1 |
| 9 | The PloyMAX frequency domain method:a new standard for modal parameter estimation显示文摘 | Bart P Herman V D A Patrick G | 2004 | Shock and Vibration2004，，11： | 1 |
| 10 | Heuristic methods for estima- ting the generalized vertex median of a weighted graph显示文摘 | TEITZ M B BART P | 1968 | Operations Research1968，16，： | 1 |
| 11 | Product shape selectivity dominates the Methanol-to-Olefins (MTO) reaction over H-SAPO-34 catalysts显示文摘 | Hereijgers Bart P C Bleken Francesca Nilsen Merete H | 2009 | Journal of Cataly- sis2009，264，1： | 1 |
| 12 | Heuristic methods for estimating the generalized vertex median of a weighted graph显示文摘 | Tietz M B Bart P | 1968 | Operations Research1968，，： | 1 |
| 13 | Chemisorptive catalytic oxidation process for SO2 from smelting waste gases by Fe(I)显示文摘 | Bart H J Ning P Sun P S | 1996 | Separations Technology61996，6，4： | 1 |
| 14 | Chromosome R in wheat breeding 显示文摘 | BARTS P | 1993 | Plant Breed Abstr1993，63，： | 1 |
| 15 | Concomitant activation of jasmonate and thylene response pathways is required for induction of a plant defensin gene in Arabidopsis显示文摘 | Thomma Bart P J Buchala A | 1998 | The Plant Cell1998，10，： | 1 |
| 16 | An HIV-1 clade C DNA prime,NYVAC boost vaccine regimen induces reliable,polyfunctional,and long-lasting T cell responses显示文摘 | Harari A Bart PA St(o)hr W Tapia G Garcia M Medjitna-Rais E Burnet S Cellerai C Erlwein O Barber T Moog C Liljestrom P Wagner R Wolf H Kraehenbuhl JP Esteban M Heeney J Frachette MJ Tartaglia J McCormack S Babiker A Weber J Pantaleo G | 2008 | J Exp Med2008，205，1： | 1 |
| 17 | Heuristic methods for estimating the generalized vertex median of a weighted graph显示文摘 | Teitz M B Bart P | 1968 | Operation Research1968，1968，： | 1 |
| 18 | Stochastic System Identification for Operational Modal Analysis显示文摘 | Bart P Buido D R | 2001 | Journal of Dynamic Systems Measurement and Control2001，123，12： | 1 |
| 19 | A Transmission Electron Microscopy Study of the Reactivity Mechanism of Tailor-made CuO Particles Toward Lithium显示文摘 | D(E)BART A DUPONT L POIZOT P | | 0，，11： | 1 |
| 20 | Competitive adsorption of nonionic surfactant and nonionic polymer on silica显示文摘 | Bart R P Frans A M L Luuk K K | 2007 | Langmuir2007，23，： | 1 |
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