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| 1 | Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil显示文摘AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA polymorphisms.RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical outcome.CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth. | Mnica Baraúna Assumpo Luisa Caricio Martins Hivana Patricia Melo Barbosa Katarine Antonia dos Santos Barile Sintia Silva de Almeida Paulo Pimentel Assumpo Tereza Cristina de Oliveira Corvelo | 2010 | World Journal of Gastroenterology2010，16，24： | 29 |
| 2 | Oral purgative and simethicone before small bowel capsule endoscopy显示文摘AIM:To evaluate small bowel cleansing quality,diagnostic yield and transit time,comparing three cleansing protocols prior to capsule endoscopy.METHODS:Sixty patients were prospectively enrolled and randomized to one of the following cleansing protocols:patients in Group A underwent a 24 h liquid diet and overnight fasting;patients in Group B followed protocol A and subsequently were administered 2 L of polyethylene glycol(PEG) the evening before the procedure;patients in Group C followed protocol B and were additionally administered 100 mg of simethicone 30 min prior to capsule ingestion.Small bowel cleansing was independently assessed by two experienced endoscopists and classified as poor,fair,good or excellent according to the proportion of small bowel mucosa under perfect conditions for visualization.When there was no agreement between the two endoscopists,the images were reviewed and discussed until a consensus was reached.The preparation was considered acceptable if > 50% or adequate if > 75% of the mucosa was in perfect cleansing condition.The amount of bubbles was assessed independently and it was considered significant if it prevented a correct interpretation of the images.Positive endoscopic findings,gastric emptying time(GET) and small bowel transit time(SBTT) were recorded for each examination.RESULTS:There was a trend favoring Group B in achieving an acceptable(including fair,good or excellent) level of cleansing(Group A:65%;Group B:83.3%;Group C:68.4%) [P = not significant(NS)] and favoring Group C in attaining an excellent level of cleansing(Group A:10%;Group B:16.7%;Group C:21.1%)(P = NS).The number of patients with an adequate cleansing of the small bowel,corresponding to an excellent or good classification,was 5(25%) in Group A,5(27.8%) in Group B and 4(21.1%) in Group C(P = 0.892).Conversely,7 patients(35%) in Group A,3 patients(16.7%) in Group B and 6 patients(31.6%) in Group C were considered to have poor small bowel cleansing(P = 0.417),with significant fluid or debris such that the examination was unreliable.The proportion of patients with a significant amount of bubbles was 50% in Group A,27.8% in Group B and 15.8% in Group C(P = 0.065).This was significantly lower in Group C when compared to Group A(P = 0.026).The mean GET was 27.8 min for Group A,27.2 min for Group B and 40.7 min for Group C(P = 0.381).The mean SBTT was 256.4 min for Group A,256.1 min for Group B and 258.1 min for Group C(P = 0.998).Regarding to the rate of complete examinations,the capsule reached the cecum in 20 patients(100%) in Group A,16 patients(88.9%) in Group B and 17 patients(89.5%) in Group C(P = 0.312).A definite diagnosis based on relevant small bowel endoscopic lesions was established in 60% of the patients in Group A(12 patients),44.4% in Group B(8 patients) and 57.8% in Group C(11 patients)(P = 0.587).CONCLUSION:Preparation with 2 L of PEG before small bowel capsule endoscopy(SBCE) may improve small bowel cleansing and the quality of visualization.Simethicone may further reduce intraluminal bubbles.No significant differences were found regarding GET,SBTT and the proportion of complete exploration or diagnostic yield among the three different cleansing protocols. | Bruno Joel Ferreira Rosa Mara Barbosa Joana Magalhes Ana Rebelo Maria Joo Moreira José Cotter | 2013 | World Journal of Gastrointestinal Endoscopy2013，5，2： | 21 |
| 3 | Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population显示文摘AIM:To study the association between Interleukin-1(IL-1)and tumor necrosis factor(TNF)-αpolymorphisms,infection by Helicobacter pylori(H pylori)and the development of gastrointestinal diseases.METHODS:Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers.The polymorphisms in IL-1βand TNF-αgenes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method(PCRRFLP)and those from IL-1RN with PCR.The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology.The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS:A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL1RN*2 was more frequent among patients with gastric ulcers and adenocarcinoma.Carriers of the allele ILRN*2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma,as well as a higher degree of inflammation and neutrophilic activity in the gastric mucosa.CONCLUSION:Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma. | Hivana Patricia Melo Barbosa Luisa Caricio Martins Sidney Emanuel Batista dos Santos Samia Demachki Mnica Baraúna Assumpo Charliana Damasceno Arago Tereza Cristina de Oliveira Corvelo | 2009 | World Journal of Gastroenterology2009，15，12： | 17 |
| 4 | Computed tomography-guided percutaneous core needle biopsy in pancreatic tumor diagnosis显示文摘AIM: To evaluate the techniques, results, and complications related to computed tomography(CT)-guided percutaneous core needle biopsies of solid pancreatic lesions.METHODS: CT-guided percutaneous biopsies of solid pancreatic lesions performed at a cancer reference center between January 2012 and September 2013 were retrospectively analyzed. Biopsy material was collected with a 16-20 G Tru-Core needle(10-15 cm; Angiotech, Vancouver, CA) using a coaxial system and automatic biopsy gun. When direct access to the lesion was not possible, indirect(transgastric or transhepatic) access or hydrodissection and/or pneumodissection maneuvers were used. Characteristics of the patients, lesions, procedures, and histologic results were recorded using a standardized form. RESULTS: A total of 103 procedures included in the study were performed on patients with a mean age of 64.8 year(range: 39-94 year). The mean size of the pancreatic lesions was 45.5 mm(range: 15-195 mm). Most(75/103, 72.8%) procedures were performed via direct access, though hydrodissection and/or pneumodissection were used in 22.2%(23/103) of cases and indirect transhepatic or transgastric access was used in 4.8%(5/103) of cases. Histologic analysis was performed on all biopsies, and diagnoses were conclusive in 98.1%(101/103) of cases, confirming3.9%(4/103) of tumors were benign and 94.2%(97/103) were malignant; results were atypical in 1.9%(2/103) of cases, requiring a repeat biopsy to diagnose a neuroendocrine tumor, and surgical resection to confirm a primary adenocarcinoma. Only mild/moderate complications were observed in 9/103 patients(8.7%),and they were more commonly associated with biopsies of lesions located in the head/uncinate process(n =8), than of those located in the body/tail(n = 1) of the pancreas, but this difference was not significant.CONCLUSION: CT-guided biopsy of a pancreatic lesion is a safe procedure with a high success rate, and is an excellent option for minimally invasive diagnosis. | Chiang J Tyng Maria Fernanda A Almeida Paula NV Barbosa Almir GV Bitencourt José Augusto AG Berg Macello S Maciel Felipe JF Coimbra Luiz Henrique O Schiavon Maria Dirlei Begnami Marcos D Guimares Charles E Zurstrassen Rubens Chojniak | 2015 | World Journal of Gastroenterology2015，21，12： | 14 |
| 5 | Fatty liver disease in severe obese patients:Diagnostic value of abdominal ultrasound显示文摘AIM:To evaluate the sensitivity and specificity of abdominal ultrasound (US) for the diagnosis of hepatic steatosis in severe obese subjects and its relation to histological grade of steatosis. METHODS: A consecutive series of obese patients, who underwent bariatric surgery from October 2004 to May 2005, was selected. Ultrasonography was performed in all patients as part of routine preoperative time and an intraoperative wedge biopsy was obtained at the beginning of the bariatric surgery. The US and histological findings of steatosis were compared, considering histology as the gold standard. RESULTS: The study included 105 patients. The mean age was 37.2 ± 10.6 years and 75.2% were female. The histological prevalence of steatosis was 89.5%. The sensitivity and specificity of US in the diagnosis of hepatic steatosis were, respectively, 64.9% (95% CI: 54.9-74.3) and 90.9% (95% CI: 57.1-99.5). The positive predictive value and negative predictive value were, respectively, 98.4% (95% CI: 90.2-99.9) and 23.3% (95% CI: 12.3-39.0). The presence of steatosis onUS was associated to advanced grades of steatosis on histology (P = 0.016). CONCLUSION: Preoperative abdominal US in our series has not shown to be an accurate method for the diagnosis of hepatic steatosis in severe obese patients. Until another non-invasive method demonstrates better sensitivity and specificity values, histological evaluation may be recommended to these patients undergoing bariatric surgery. | Alessandro de Moura Almeida Helma Pinchemel Cotrim Daniel Batista Valente Barbosa Luciana Gordilho Matteoni de Athayde Adimeia Souza Santos Almir Galvo Vieira Bitencourt Luiz Antonio Rodrigues de Freitas Adriano Rios Erivaldo Alves | 2008 | World Journal of Gastroenterology2008，14，9： | 10 |
| 6 | Nonalcoholic fatty liver disease in asymptomatic Brazilian adolescents显示文摘AIM:To evaluate the prevalence and clinical characteristics of Nonalcoholic fatty liver disease(NAFLD) among asymptomatic Brazilian adolescents. METHODS:Transversal observational study included asymptomatic adolescents with central obesity from private and public schools in Salvador-Bahia,northeastern Brazil.The children answered a questionnaire that included age,gender,race,and medical history,and were submitted to a complete physical exam and abdominal ultrasound.Biochemical exams included:ALT,AST,GGT,C reactive protein(CRP),fasting glucose,insulin,cholesterol and triglycerides.Criteria for NAFLD included:the presence of steatosis in ultrasound and/or high level of ALT,negative or occasional historic of intake of alcohol(≤140 g/wk),negative investigation for hepatitis A,B,C,auto-immune hepatitis,Wilson disease and hemochromatosis.RESULTS:From October,2005 to October,2006,the study included 1801 subjects between 11 and 18 years of age and a mean age of 13.7±2.0 years.One hundred ninety-nine had central obesity.The prevalence of NAFLD was 2.3%,most of whom were male and white.Insulin resistance(IR)was observed in 22.9% of them and had positive correlations with ALT and GGT(P<0.05).Elevated CRP was observed in 6.9% of the cases;however,it was not associated with WC,IR or liver enzymes. CONCLUSION:The prevalence of NAFLD in Brazilian adolescents was low.The ethnicity may have influence this frequency in the population studied,which had a large proportion of African descendents. | Raquel Rocha Helma Pinchemel Cotrim Almir Galvo Vieira Bitencourt Daniel Batista Valente Barbosa Adméia Souza Santos Alessandro de Moura Almeida Bruno Cunha Isabel Guimares | 2009 | World Journal of Gastroenterology2009，15，4： | 2 |
| 7 | Clinical hematological and biochemical parameters in Swiss,BALB/c,C57BL/6 and B6D2F1 Mus musculus显示文摘Background:Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions.Various lineages of mice have been used to investigate diseases and new therapeutic strategies,and,consequently,hematological and biochemical tests in these laboratory animals are essential to validate scientific studies.Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice.Methods:We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss(heterogeneous),BALB/c and C57BL/6(isogenic),and B6D2F1(hybrid),totaling 160 mice.Analysis were standardized using the systems pocH-100iV Diff™for 19 hematological parameters and VITROS®350 for 12 biochemical parameters.Results:Results are shown as means and standard deviation,grouped by lineage and genre.Comparing the values obtained in this study with the values from previous studies,some variations were detected,which could be explained by differences in methodologies or individual variability.Conclusion:Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary,and emphasises the importance of considering variations influenced by gender,lineage and genotype in the choice of the best experimental model. | Giorgio Silva-Santana Juliet Cunha Bax Débora Cristina Silva Fernandes Daniela Tendler Leibel Bacellar Cleber Hooper Alexandre Alves Souza Oliveira Dias Cristina Barbosa Silva Aline Moreira de Souza Simone Ramos Ricardo Alexandre Santos Thainara Ramos Pinto Mariana Antunes Ramão Ana Luíza Mattos-Guaraldi | 2020 | Animal Models and Experimental Medicine2020，3，4： | 2 |
| 8 | A physical map of large segments of pig chromosome 7q11-q14:comparative analysis with human chromosome 6p21显示文摘 | BARBOSA A DEMEURE O URIEN C | 2004 | Mamm Genome2004，15，12： | 1 |
| 9 | Prevalance of anemia and its association with cardio-renal syndrome显示文摘 | Silva R P Barbosa P H Kimura O S | | 0，，02： | 1 |
| 10 | Azo dye decolorization by optimized cross linked enzyme aggregates (CLEAs) of a royal palm (Roystonea regia) peroxidase 显示文摘 | GRATERON C BARBOSA O RUEDA N | | J Biotechnol0，131，2： | 1 |
| 11 | Brazing parameters determine the degradation and mechanical behaviour of alumina/titanium brazed joints显示文摘 | Paiva O C Barbosa M A | 2000 | Journal of Materials Science2000，35，5： | 1 |
| 12 | Monofunctional catalase P of Paracoccidioides brasiliensis: identification, characterization, molecular cloning and expression analysis显示文摘 | Moreira SF Bail o AM Barbosa MS | 2004 | Yeast2004，21，2： | 1 |
| 13 | Dissemination strategy for immuni- zing scale-free networks 显示文摘 | STAUFFER A O BARBOSA V C | 2006 | Physical Review E2006，74，05： | 1 |
| 14 | Effects of the vitamin E in catalase activities in hippocampus after status epi- lepticus induced by pilocarpine in wistar rats显示文摘 | Barros D O Xavier S M L Barbosa C O etal | 2007 | Neuroscencei Letters2007，416，3： | 1 |
| 15 | An efficient parallel optimization algorithm for the Token Bucket control mechanism显示文摘 | Ahmed N U Lu X Barbosa L O | 2006 | Computer Communications2006，29，12： | 1 |
| 16 | Screening analysis of natural gas with respect to methane content by near-infrared spectrometry显示文摘 | Hebertty V. Dantas Mayara F. Barbosa Elaine C.L. Nascimento Pablo N.T. Moreira Roberto K.H. Galv?o Mário C.U. Araújo | 2014 | Microchemical Journal2014，，： | 1 |
| 17 | Increased interleukin 10,tumor necrosis factor alpha,and interleukin 6 levels in blister fluid of toxic epidermal necrolysis显示文摘 | Correia O Delgado L Barbosa IL | 2002 | J Am Acad Dermatol2002，47，1： | 1 |
| 18 | Characterization of metal/mould interface on investment casting of γ-TiAl显示文摘 | BARBOSA J PUGA H RIBEIRO C S TEODORO O M N D MONTEIRO A C | 2006 | International Journal of Cast Metals Research2006，19，6： | 1 |
| 19 | Stability, antimicrobial activity, and effect of nisin on the physico - chemical properties of fruit juices 显示文摘 | Junior A A D O Moura T R D Barbosa A A T | 2015 | International Journal of Food Microbiology2015，211，： | 1 |
| 20 | Ovarian function during use of a levonorgestrel - releasing IUD 显示文摘 | Barbosa I Bakos O Olsson SE | 1990 | Contraception1990，42，1： | 1 |
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