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| 1 | MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium显示文摘Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and identified differentially expressed microRNAs in bone marrow- derived mesenchymal stem cells induced by Schwann cell-conditioned medium, and explored targets and related pathways involved in their differentiation into neuronal-like cells. Primary bone marrow-derived mesenchymal stem cells were isolated from femoral and tibial bones, while primary Schwann cells were isolated from bilateral saphenous nerves. Bone marrow-derived mesenchymal stem cells were cultured in unconditioned (control group) and Schwann cell-conditioned medium (bone marrow-derived mesenchymal stem cell + Schwann cell group). Neuronal differentiation of bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium was observed by time-lapse imaging. Upon induction, the morphology of bone marrow-derived mesencaymal stem cells changed into a neural shape with neurites. Results of quantitative reverse transcription-polymerase chain reaction revealed that nestin mRNA expression was upregulated from 1 to 3 days and downregulated from 3 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. Compared with the control group, microtubule-associated protein 2 mRNA expression gradually increased from 1 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. After 7 days of induction, microRNA analysis iden:ified 83 significantly differentially expressed microRNAs between the two groups. Gene Ontology analysis indicated enrichment of microRNA target genes for neuronal projection development, regulation of axonogenesis, and positive regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that Hippo, Wnt, transforming growth factor-beta, and Hedgehog signaling pathv/ays were potentially associated with neural differentiation of bone marrow-derived mesenchymal stem cells. This study, which carried out successful microRNA analysis of neuronal-like cells differentiated from bone marrow-derived mesenchymal stem cells by Schwann cell induction, revealed key microRNAs and pathways involved in neural differentiation of bone marrow-derived mesenchymal stem cells. All protocols were approved by the Animal Ethics Committee of Institute of Radiation Medicine, Chinese Academy of Medical Sciences on March 12, 2017 (approval number: DWLI-20170311). | Zhi-Jian Wei Bao-You Fan Yang Liu Han Ding Hao-Shuai Tang Da-Yu Pan Jia-Xiao Shi Peng-Yuan Zheng Hong-Yu Shi Heng Wu Ang Li Shi-Qing Feng | 2019 | Neural Regeneration Research2019，14，8： | 13 |
| 2 | Prognostic effect analysis of molecular subtype on young breast cancer patients显示文摘Objective: To make a prognostic effect analysis of molecular subtype on young breast cancer patients.Methods: Totally 187 cases of young breast cancer patients less than 40 years old treated in Obstetrics and Gynecology Hospital of Fudan University between June 2005 and June 2011 were included in our study. We described their clinical-pathological characteristics, disease-free survival(DFS) rate, and overall survival(OS) rate after a median follow-up period of 61 months. The factors associated with prognosis were also evaluated by univariate and multivariate analyses.Results: All patients were premenopausal, with an average age of 35.36±3.88 years old. The mean tumor size was 2.43±1.53 cm. Eighty-one cases had lymph node metastasis(43.3%), 126 cases had lymphovascular invasion(67.4%), and 125 cases had histological grade III(66.8%) disease. Twenty-seven cases(14.4%) were Luminal A subtype, 99 cases(52.9%) were Luminal B subtype, 29 cases(15.5%) were human epidermal growth factor receptor 2(HER-2) overexpression subtype, while 32 cases(17.1%) were triple negative breast cancer(TNBC) subtype according to 2013 St Gallen expert consensus. One hundred and thirty-five cases underwent mastectomy whereas 52 cases had breast-conserving surgery. One hundred and seventy-eight cases underwent adjuvant or neoadjuvant chemotherapy. Recurrence or metastasis occurred in 29 cases, 13 of which died. The 5-year DFS and OS rates were 84% and 92%. Multivariate analysis showed that nodal status(P=0.041) and molecular subtype(P=0.037) were both independent prognostic factors of DFS, while nodal status(P=0.037) and TNBC subtype(P=0.048) were both independent prognostic factors of OS. Conclusions: Molecular subtype is an independent prognostic factor of young breast cancer patients. TNBC has a high risk of relapse and death. | Hong-Liang Chen Ang Ding Fu-Wen Wang | 2015 | Chinese Journal of Cancer Research2015，27，4： | 8 |
| 3 | Distinct Contributions of Replication and Transcription to Mutation Rate Variation of Human Genomes显示文摘Here, we evaluate the contribution of two major biological processes-DNA replication and transcription-to mutation rate variation in human genomes. Based on analysis of the public human tissue transcriptomics data, high-resolution replicating map of Hela cells and dbSNP data, we present significant correlations between expres-sion breadth, replication time in local regions and SNP density. SNP density of tissue-specific (TS) genes is sig-nificantly higher than that of housekeeping (HK) genes. TS genes tend to locate in late-replicating genomic re-gions and genes in such regions have a higher SNP density compared to those in early-replication regions. In addi-tion, SNP density is found to be positively correlated with expression level among HK genes. We conclude that the process of DNA replication generates stronger mutational pressure than transcription-associated biological processes do, resulting in an increase of mutation rate in TS genes while having weaker effects on HK genes. In contrast, transcription-associated processes are mainly responsible for the accumulation of mutations in highly-expressed HK genes. | Peng Cui Feng Ding Qiang Lin Lingfang Zhang Ang Li Zhang Zhang Songnian Hu Jun Yu | 2012 | Genomics, Proteomics & Bioinformatics2012，10，1： | 5 |
| 4 | Preparation and drug release behaviors of nimodipine-loaded poly (eaprolaetone)-poly (ethylene oxide)- polylactide amphiphilic copolymer nanoparticles 显示文摘 | I-tU Y J]ANG X DING Y | 2003 | Biomaterials2003，24，13： | 1 |
| 5 | Efficient Scheduling with Random Network Coding in Multi- Relay Wireless Network显示文摘 | DING Liang-hui BI Yahb YANG Feng SUN Da-jiab QIAN Li- ang | 2014 | Journal of Shanghai Jiaotong universi- ty2014，1，： | 1 |
| 6 | The short-chain fatty acid receptor GPR43 is transcriptionally regulated by XBP1 in human monocytes显示文摘 | Ang Z Er JZ Ding JL | 2015 | Scien- tific Reports2015，5，： | 1 |
| 7 | A novel interface for interractive exploration of DTI fibers显示文摘 | CHEN Wei DING Zi' ang ZHANG Song | 2012 | IEEE Transations on Visual- ization and Computer Graphics2012，15，6： | 1 |
| 8 | Hypoxia-Inducible mir-210 Regulates Normoxic Gene Expression Involved in Tumor Initiation显示文摘 | Xin Huang Lianghao Ding Kevin L. Bennewith Ricky T. Tong Scott M. Welford K. Kian Ang Michael Story Quynh-Thu Le Amato J. Giaccia | 2009 | Molecular Cell2009，，6： | 1 |
| 9 | Improvement of exopolysaccharide production by macro-fungus Aurlcularia auricula in submerged culture显示文摘 | WU Jing DING Zhongyang ZH ANG Kechang | 2006 | Enzyme and Microbial Technology2006，39，4： | 1 |
| 10 | Weekly paelitaxel with con- current radiotherapy followed by adjuvant chemotherapy in local lyadiother advanced nasopharyngeal carcinoma 显示文摘 | YIu W Ding W ~ang H | 2009 | Oncol2009，93，3： | 1 |
| 11 | Government ownership and the performance of government-linked companies: The case of Singapore 显示文摘 | Ang J S Ding D K | 2006 | Journal of Multinational Financial Management2006，16，7： | 1 |
| 12 | δ-Calculus:A New Approach to Quantifying Location Privacy显示文摘With the rapid development of mobile wireless Internet and high-precision localization devices,location-based services(LBS)bring more convenience for people over recent years.In LBS,if the original location data are directly provided,serious privacy problems raise.As a response to these problems,a large number of location-privacy protection mechanisms(LPPMs)(including formal LPPMs,FLPPMs,etc.)and their evaluation metrics have been proposed to prevent personal location information from being leakage and quantify privacy leakage.However,existing schemes independently consider FLPPMs and evaluation metrics,without synergizing them into a unifying framework.In this paper,a unified model is proposed to synergize FLPPMs and evaluation metrics.In detail,the probabilistic process calculus(calledδ-calculus)is proposed to characterize obfuscation schemes(which is a LPPM)and integrateα-entropy toδ-calculus to evaluate its privacy leakage.Further,we use two calculus moving and probabilistic choice to model nodes’mobility and compute its probability distribution of nodes’locations,and a renaming function to model privacy leakage.By formally defining the attacker’s ability and extending relative entropy,an evaluation algorithm is proposed to quantify the leakage of location privacy.Finally,a series of examples are designed to demonstrate the efficiency of our proposed approach. | Lihua Yin Ran Li Jingquan Ding Xiao Li Yunchuan Guo Huibing Zhang Ang Li | 2020 | Computers, Materials & Continua2020，，6： | 0 |
| 13 | Dual-sized hollow particle incorporated fibroin thermal insulating coatings on catheter for cerebral therapeutic hypothermia显示文摘Selective endovascular hypothermia has been used to provide cooling-induced cerebral neuroprotection,but current catheters do not support thermally-insulated transfer of cold infusate,which results in an increased exit temperature,causes hemodilution,and limits its cooling efficiency.Herein,air-sprayed fibroin/silica-based coatings combined with chemical vapor deposited parylene-C capping film was prepared on catheter.This coating features in dual-sized-hollow-microparticle incorporated structures with low thermal conductivity.The infusate exit temperature is tunable by adjusting the coating thickness and infusion rate.No peeling or cracking was observed on the coatings under bending and rotational scenarios in the vascular models.Its efficiency was verified in a swine model,and the outlet temperature of coated catheter(75μm thickness)was 1.8-2.0◦C lower than that of the uncoated one.This pioneering work on catheter thermal insulation coatings may facilitate the clinical translation of selective endovascular hypothermia for neuroprotection in patients with acute ischemic stroke. | Ming Li Yuan Gao Miaowen Jiang Hongkang Zhang Yang Zhang Yan Wu Wenhao Zhou Di Wu Chuanjie Wu Longfei Wu Luzi Bao Xiaoxiao Ge Zhengfei Qi Ming Wei Ang Li Yuchuan Ding Jicheng Zhang Guangzhen Pan Yu Wu Yan Cheng Yufeng Zheng Xunming Ji | 2023 | Bioactive Materials2023，，8： | 0 |
| 14 | Focused crawling strategies based on ontologies and simulated annealing methods for rainstorm disaster domain knowledge显示文摘At present,focused crawler is a crucial method for obtaining effective domain knowledge from massive heterogeneous networks.For most current focused crawling technologies,there are some difficulties in obtaining high-quality crawling results.The main difficulties are the establishment of topic benchmark models,the assessment of topic relevance of hyperlinks,and the design of crawling strategies.In this paper,we use domain ontology to build a topic benchmark model for a specific topic,and propose a novel multiple-filtering strategy based on local ontology and global ontology(MFSLG).A comprehensive priority evaluation method(CPEM)based on the web text and link structure is introduced to improve the computation precision of topic relevance for unvisited hyperlinks,and a simulated annealing(SA)method is used to avoid the focused crawler falling into local optima of the search.By incorporating SA into the focused crawler with MFSLG and CPEM for the first time,two novel focused crawler strategies based on ontology and SA(FCOSA),including FCOSA with only global ontology(FCOSA_G)and FCOSA with both local ontology and global ontology(FCOSA_LG),are proposed to obtain topic-relevant webpages about rainstorm disasters from the network.Experimental results show that the proposed crawlers outperform the other focused crawling strategies on different performance metric indices. | Jingfa LIU Fan LI Ruoyao DING Zi’ang LIU | 2022 | Frontiers of Information Technology & Electronic Engineering2022，23，8： | 0 |
| 15 | Recent advances on the total synthesis of alkaloids in China's Mainland显示文摘Alkaloids are a large family of natural products that mostly contain basic nitrogen atoms. Because of their intriguing structures and important functions, they have long been popular targets for synthetic organic chemists. China's chemists have made significant progress in the area of alkaloid synthesis over past decades. In this article, selected total syntheses of alkaloids from research groups in China's Mainland during the period 2011–16 are highlighted. | Yong Li Jian Li Hanfeng Ding Ang Li | 2017 | National Science Review2017，4，3： | 0 |
| 16 | Clinical features of 162 fatal cases of COVID-19:a multi-center retrospective study显示文摘Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan. | Xianlong Zhou Guoyong Ding Qing Fang Jun Guo Luyu Yang Ping Wang Shou-Zhi Fu Ang Li Jian Xia Jiangtao Yu Jianyou Xia Min Ma Zhuanzhuan Hu Lei Huang Ruining Liu Cheng Jiang Shaoping Li Mingxia Yu Xizhu Xu Yan Zhao Quan Hu Weijia Xing Zhigang Zhao | 2022 | Emergency and Critical Care Medicine2022，2，3： | 0 |
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