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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Is it possible to treat recurrent clubfoot with the Ponseti technique after posteromedial release?: a preliminary study显示文摘 | Nogueira MP Ey Batlle AM Alves CG | 2009 | Clin Orthop Relat Res2009，467，5： | 1 |
| 3 | Perioperative blood glucose level and postoperative complications in pediatric cardiac surgery显示文摘 | Alves RL Cerqueira MP Kraychete NC | | 0，，05： | 1 |
| 4 | Enl and Wnt signaling in mid- brain dopaminergic neuronal development 显示文摘 | Alves dos Santos MT Smidt MP | 2011 | Neural Dev2011，6，： | 1 |
| 5 | En1 and Wnt signaling in midbrain dopaminergic neuronal development显示文摘 | Alves DSM Smidt MP | 2011 | Neural Dev2011，6，： | 1 |
| 6 | Wild-type and mutated presenilins 2 trigger p53-dependent apoptosis and down-regulate presenilin 1 expression in HEK293 human cells and in murine neurons显示文摘 | Alves da Costa C Paitel E Mattson MP | 2002 | Proc Natl Acad Sci USA2002，99，6： | 1 |
| 7 | Cetuximab plus platinum?based chemotherapy in head and neck squamous cell carcinoma: a ret?rospective study in a single comprehensive European cancer institution显示文摘 | de Mello RA Gems S Alves MP | 2014 | PLoS One2014，9，86： | 1 |
| 8 | Identification of the role of RIG-I, MDA-5 and TLR3 in sensing RNA viruses in porcine epithelial cells using lentivirus driven RNA interference 显示文摘 | Hasser L Alves MP Ruggli N | 2011 | Virus Res2011，159，1： | 1 |
| 9 | Liver injury and viremia in mice infected with dengue-2 virus 显示文摘 | Paes MV Pinhao AT Barreto DF Costa SM Oliveira MP Nogueira AC Takiya CM Farias-Filho JC Schatzmayr HG Alves AM Barth OM | 2005 | Virology2005，338，2： | 1 |
| 10 | Human skin penetration and distribution of nimesulide from hydrophilie gels containing nanocarriers 显示文摘 | ALVES MP SCARRONE AL GUTERRES SS | 2007 | Int J Pharm2007，341，12： | 1 |
| 11 | Identification of the role of RIG-I,MDA-5 and TLR3 in sensing RNA viruses in porcine epithelial cells using lentivirus-driven RNA interference显示文摘 | Hüsser L Alves MP Ruggli N | 2011 | Virus Res2011，159，1： | 1 |
| 12 | Hereditary warfarin resistance : investigation of a rare phenomenon 显示文摘 | Alving BM Strickler MP Knight RD | 1985 | Arch Intern Med1985，145，： | 1 |
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