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您的检索式：作者名="Alvarez Ruth"
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| 1 | Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers显示文摘Mesenchymal stem cell(MSC)-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations:CD51/CD140 a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells(DMSCs)from heterogeneous periodontal ligament cells(PDLCs). Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51^+/CD140a^+, 0.8% were CD271^+, and 2.4% were STRO-1^+/CD146^+. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271^+ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine. | Ruth Alvarez Hye-Lim Lee Cun-Yu Wang Christine Hong | 2015 | International Journal of Oral Science2015，7，4： | 8 |
| 2 | Single CD271 marker isolates mesenchymal stem cells from human dental pulp显示文摘Mesenchymal stem cells(MSCs) are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow(BMSCs), adult MSCs are isolated from craniofacial tissues including dental pulp tissues(DPs) using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cells(DMSCs). In this study, we used different combinations of surface markers(CD51/CD140 a, CD271, and STRO-1/CD146) to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells(DPCs) obtained from DP and compared their capacity to undergo multilineage differentiation.Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51^+/CD140a^+, 10.6% were CD271^+, and 0.3% were STRO-1^+/CD146^+. Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271^+ DMSCs demonstrated the greatest odontogenic potential.While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271^+ DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine. | Ruth Alvarez Hye-Lim Lee Christine Hong Cun-Yu Wang | 2015 | International Journal of Oral Science2015，7，4： | 1 |
| 3 | Genetic variation in the renin-angiotensin system and athletic performance显示文摘 | RUTH ALVAREZ NICOLAS TERRADOS RAQUEL ORTOLANO | 2000 | Eur J Appl Physiol2000，82，： | 1 |
| 4 | Study of the influnce of the basicity and chromate-chloride anionic composition in the synthesis of the cancrinite-sodalite system显示文摘 | Alvarez Ruth Moy Beatriz | 2008 | Journal of Materials Science2008，43，1： | 1 |
| 5 | Class IIa Histone Deacetylases Are Hormone-Activated Regulators of FOXO and Mammalian Glucose Homeostasis显示文摘 | Maria M. Mihaylova Debbie S. Vasquez Kim Ravnskjaer Pierre-Damien Denechaud Ruth T. Yu Jacqueline G. Alvarez Michael Downes Ronald M. Evans Marc Montminy Reuben J. Shaw | 2011 | Cell2011，，4： | 1 |
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