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| 1 | Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC. | Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel | 2017 | World Journal of Stem Cells2017，9，9： | 4 |
| 2 | Comparison of various advanced oxidation processes for the degradation of 4-chloro- 2-nitrophenol 显示文摘 | Saritha P Aparna C Himabindu V | 2007 | Journal of Hazardous Materials2007，149，3： | 1 |
| 3 | Comparison of various advanced oxidation processes for the degradation of 4-chloro-2-nitrophenol显示文摘 | SARITHA P APARNA C HIMABINDU V | 2007 | Journal of Hazardous Materials2007，149，3： | 1 |
| 4 | Effect of curcumin on gelatinase A ( MMP - 2 ) activity in B16F10 melanoma cells 显示文摘 | ANIRUDDHA B JAYATI C APARNA M | 2010 | Cancer Letters2010，211，： | 1 |
| 5 | Culture of human cervical cancer cells,SiHa,in the presence of fibronectin activates MMP -2显示文摘 | Aparna M Jayati C Aniruddha B | 2006 | J Cancer Res Clin Onool2006，132，8： | 1 |
| 6 | Fuzzy linear program- ming under interval uncertainty based on IFS representation 显示文摘 | DIPTI D SURESH C APARNA M | 2012 | Fuzzy Sets and Systems2012，188，1： | 1 |
| 7 | Stabilisation and solidification technologies for the remediation of contaminated soils and sediments : An overview显示文摘 | Raj D Suman S Aparna C | 2005 | Land Contamination and Reclamation2005，13，1： | 1 |
| 8 | Stabilization and solidification technologies for the remediation of contaminated soils and sediments: An overview显示文摘 | Suman Raj D S Aparna C Rekha P | 2005 | Land Contamination and Reclamation2005，13，1： | 1 |
| 9 | Comparison of Various Advanced Oxidation Processes for the Degradation of 4-Chloro-2 Nitrophenol显示文摘 | Saritha P Aparna C Himabindu V | 2007 | J Hazard Mater2007，149，3： | 1 |
| 10 | Stabilization and solidifica- tion technologies for the remediation of contaminated soils and sedi- ments:An overview显示文摘 | Suman Raj D S Aparna C Rekha P | 2005 | Land Contamination and Reclamation2005，13，1： | 1 |
| 11 | Microbial metabolism of pyrazines显示文摘 | Rajini K S Aparna P Sasikala C Ramana C V | 2011 | Crit Rev Microbiol2011，37，： | 1 |
| 12 | Silicene beyond mono-layers—different stacking configurations and their properties显示文摘 | C Kamal Aparna Chakrabarti Arup Banerjee S K Deb | 2013 | Journal of Physics: Condensed Matter2013，，： | 1 |
| 13 | Stabilization and solidification technologies for the remediation of contaminated soils and sediments:An overview显示文摘 | Suman Raj D S Aparna C Rekha P | 2005 | Land Contamination and Reclamation2005，13，1： | 1 |
| 14 | Degradative oxidation of2,4,6 trichlorophenol using advanced oxidation processes-A comparative study显示文摘 | Saritha P Raj D S Aparna C | 2009 | Water Air and Soil Pollution2009，200，14： | 1 |
| 15 | Stabilization and solidifica- tion technologies for the remediation of contaminated soils and sedi- ments:An overview显示文摘 | Suman Raj D S Aparna C Rekha P | 2005 | Land Contamination and Reclamation2005，13，1： | 1 |
| 16 | Culture of human cervical cancer cells,SiHa,in the presence of fibronectin activates MMP-2显示文摘 | Aparna M Jayati C Anirnddha B | 2006 | Cancer Res Clin Oncol2006，132，8： | 1 |
| 17 | Comparison of Various Advanced Oxidation Processes for the Degra- dation of 4-ehloro-2 Nitrophenol显示文摘 | Saritha P Aparna C Himabindu V | 2007 | Journal of Hazard- ous Materials2007，149，3： | 1 |
| 18 | Epigenetic thera- py of cancer with histone deacetylase inhibitors显示文摘 | Lakshmaiah K C Jacob L A Aparna S | 2014 | J Cancer Res Ther2014，10，3： | 1 |
| 19 | Constitutive expression of protective antigen gene of Bacillus anthracis in Escherichia coli 显示文摘 | Vibha C Aparna S Waheed SM | 2001 | Biochem Biophy Res Communications2001，283，： | 1 |
| 20 | Enhancement of dissolution of irbesartan using liquisolid technology显示文摘 | Santosh P Aparna C Srinivas P | 2012 | Int J Pharm Technol2012，4，1： | 1 |
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