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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | Initial experience with EUS-guided cholangiopancreatography for biliary and pancreatic duct drainage: a Spanish national survey显示文摘 | Juan J. Vila Manuel Pérez-Miranda Enrique Vazquez-Sequeiros Monder Abu-Suboh Abadia Antonio Pérez-Millán Ferrán González-Huix Joan Gornals Julio Iglesias-Garcia Carlos De la Serna José R. Aparicio José C. Subtil Alberto álvarez Felipe de la Morena Jesús G | 2012 | Gastrointestinal Endoscopy2012，，6： | 2 |
| 3 | Superovlatory Response in Bos Indi- cus Breeds,Breeds,Borders or Lack of Knowledge 显示文摘 | Armaser R D Aparicio N Nader C | 2001 | Archives of Animal Breeding2001，44，： | 1 |
| 4 | Changes of banana starch by chemical and physical modification显示文摘 | Waliszewski K N Aparicio M A Bello L A | 2003 | Carbohydrate Polymers2003，52，3： | 1 |
| 5 | Nutritional status of hemodialysis patients: A French nutritional cooperative study显示文摘 | APARICIO M CANO N CHAUVEAU P | 1999 | Nephrol Dail Transplant1999，14，： | 1 |
| 6 | Changes of Banana Starch by Chemical and Physical Modification 显示文摘 | Waliszewski N K Aparicio M A Bello L A | 2003 | CarbohydrPolym2003，52，3： | 1 |
| 7 | Changes of banana starch by chemical and physical modification显示文摘 | Waliszewski K N Aparicio M A Bello L A Monroy J A | 2003 | Carbohydrate Polymers2003，52，3： | 1 |
| 8 | Spectral vegetation indices as non-destructive tools for determining durum wheat yield显示文摘 | Aparicio N Villegas D Casadesus J | 2000 | Agronomy Journal2000，92，： | 1 |
| 9 | Seedling development and biomass as affected by seed size and morphology in durum wheat显示文摘 | APARICIO N VILLAGAS D ARAUS J L | 2002 | Journal of Agricultural Science2002，139，2： | 1 |
| 10 | Thick sol-gel coatings based on the B2O3-SiO2 system显示文摘 | Villegas M A Aparicio M A Durán A | 1997 | Journal of Non-Crystalline Solids1997，，218： | 1 |
| 11 | Predictivepower control for PV plants with energy storage显示文摘 | Perez E Beltran H Aparicio N | 2013 | IEEE Transactions on Sustainable Energy2013，4，2： | 1 |
| 12 | Biomass accumulation and main stem elongation of durum wheat grown under Mediterranean conditions显示文摘 | Villegas D Aparicio N Blanco R | 2001 | Annals of Botany2001，88，： | 1 |
| 13 | Nutritional status of he- modialysis patients : A French National Cooperative Study 显示文摘 | Aparicio M Cano N Chauveau P | 1999 | Nephrol Dial Transplant1999，14，7： | 1 |
| 14 | On the representation of random packings of spheres for sintering simulations显示文摘 | APARICIO N D COCKS C F | 1995 | Acta Metall Mater1995，43，10： | 1 |
| 15 | Spectral Vegetation Indices as Nondestructive Tools for Determining Durum Wheat Yield显示文摘 | Aparicio N Villegas D Casadesus J | 2000 | Agronomy Journal2000，92，： | 1 |
| 16 | LCC-HVDC connection of offshore wind farms with reduced filter banks显示文摘 | Blasco-Gimenez R Aparicio N Ano-Villalba S | 2013 | IEEE Transactions on Industrial Power Electronics2013，60，6： | 1 |
| 17 | Biomassaccumulation and main stem elongation of durum wheatgrown under Mediterranean conditions显示文摘 | Villegas D Aparicio N Blanco R | 2001 | Annals of Botany2001，88，4： | 1 |
| 18 | Isotopic Discrimination as a Tool for Organic Farming Certification in Sweet Pepper显示文摘 | del Amor Francisco M Navarro Joaquín Aparicio Pedro M | 2008 | Journal of Environmental Quality2008，，1： | 1 |
| 19 | ESPEN Guidelines on parenteral nutrition: adult renal failure显示文摘 | Cano N J M Aparicio M Brunori G | 2009 | Clin Nutr2009，28，4： | 1 |
| 20 | Spectral vegetation indices as non destructive tools for determining durum wheat yield显示文摘 | Aparicio N Villegas D Casadesus J | 2000 | Agronomy Journal2000，92，： | 1 |
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