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| 1 | Incidence and characteristics of HBV reactivation in hematological malignant patients in south Egypt显示文摘AIM:To investigate characteristics of hepatitis B virus(HBV)implicated in HBV reactivation in patients with hematological malignancies receiving immunosuppressive therapy.METHODS:Serum samples were collected from 53 patients with hematological malignancies negative for hepatitis B surface antigen(HBsAg)before the start of and throughout the chemotherapy course.HBV reactivation was diagnosed when the HBsAg status changed from negative to positive after the initiation of chemotherapy and/or when HBV DNA was detected by realtime detection polymerase chain reaction(RTD-PCR).For detecting the serological markers of HBV infection,HBsAg as well as antibodies to the core antigen(antiHBc)and to the surface antigen were measured in the sera by CEIA.Nucleic acids were extracted from sera,and HBV DNA sequences spanning the S gene were amplified by RTD-PCR.The extracted DNA was further subjected to PCR to amplify the complete genome as well as the specific genomic sequences bearing the enhancerⅡ/core promoter/pre-core/core regions(nt1628-2364).Amplicons were sequenced directly.RESULTS:Thirty-five(66%)of the 53 HBsAg-negative patients were found to be negative serologically for antiHBc,and the remaining 18(34%)patients were positive for anti-HBc.Five of the 53(9.4%)patients with hematologic malignancies experienced HBV reactivation.Genotype D1 was detected in all five patients.Four types of mutant strains were detected in the S gene product of HBV strains and were isolated from 3 patients with HBV reactivation:T/S120,L143,and I126.HBV DNA was detected in the pretreatment HBsAg-negative samples in one of the five patients with HBV reactivation.In this patient,sequences encompassing the HBV full genome obtained from sera before the start of chemotherapy and at the time of de novo HBV hepatitis were detected and it showed 100%homology.Furthermore,in the phylogenetic tree,the sequences were clustered together,thereby indicating that this patient developed reactivation from an occult HBV infection.CONCLUSION:Past infection with HBV is a risk factor for HBV reactivation in Egypt.Mandatory anti-HBc screening prior to chemotherapy in patients with hematological malignancies is recommended. | Abeer Elkady Sahar Aboulfotuh Elsayed Mostafa Ali Douaa Sayed Nashwa M Abdel-Aziz Amany M Ali Shuko Murakami Sayuki Iijima Yasuhito Tanaka | 2013 | World Journal of Gastroenterology2013，19，37： | 7 |
| 2 | Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt显示文摘AIM To investigate the prevalence and virological characteristics of occult hepatitis B virus(HBV) infections in patients with hematological malignancies in South Egypt.METHODS Serum samples were collected from 165 patients with hematological malignancies to monitor titers of HBV DNA, hepatitis B surface antigen(HBs Ag), and antibodies to HBV core(anti-HBc) and surface antigens. Serum samples negative for HBs Ag and positive for anti-HBc were subjected to nucleic acid extraction and HBV DNA detection by real-time polymerase chain reaction. DNA sequences spanning the S region were analyzed in cases with occult HBV infection. In vitro comparative study of constructed 1.24-fold wild type and S protein mutant HBV genotype D clones was further performed. RESULTS HBV DNA was detected in 23(42.6%) of 54 patients with hematological malignancies who were HBsA g negative, but anti-HBc positive, suggesting the presence of occult HBV infection. The complete HBV genome was retrieved from 6 occult HBV patients, and P120 T and S143 L were detected in 3 and 2 cases, respectively. Site directed mutagenesis was done to produce 1.24-fold genotype D clones with amino acid mutations T120 and L143. The in vitro analyses revealed that a lower level of extracellular HBsA g was detected by chemiluminescence enzyme immunoassay(CLEIA) with the clone containing T120 mutation, compared with the wild type or the clone with S143 L mutation despite the similar levels of extracellular and intracellular HBs Ag detected by Western blot. Southern blot experiments showed that the levels of intracellular HBV DNA were not different between these clones. CONCLUSION Occult HBV infection is common in patients with hematological malignancies and associated with P120 T and S143 L mutations. 120 T mutation impairs the detection of HBsA g by CLEIA. | Abeer Elkady Sayuki Iijima Sahar Aboulfotuh Elsayed Mostafa Ali Douaa Sayed Nashwa M Abdel-Aziz Amany M Ali Shuko Murakami Masanori Isogawa Yasuhito Tanaka | 2017 | World Journal of Hepatology2017，9，9： | 2 |
| 3 | Effectiveness of hepatitis B virus vaccination program in Egypt:Multicenter national project显示文摘AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory. | Iman I Salama Samia M Sami Zeinab Nabil Ahmed Said Manal H El-Sayed Lobna A El Etreby Thanaa M Rabah Dalia M Elmosalami Amany T Abdel Hamid Somaia I Salama Aida M Abdel Mohsen Hanaa M Emam Safaa M Elserougy Amal I Hassanain Naglaa F Abd Alhalim Fatma A Shaaban Samia A Hemeda Nihad A Ibrahim Ammal M Metwally | 2015 | World Journal of Hepatology2015，7，22： | 2 |
| 4 | Yield and quality of maize as affected by slow-release nitrogen in newly reclaimed sand- y soil显示文摘 | Amany A B Zeidan M S Hozayn M | 2006 | American-Eurasian Journal of Agricultural & Environmental2006，1，3： | 1 |
| 5 | Thin-film thermo- couples based on the system In203-SnO2 显示文摘 | CHEN X GREGORY O J AMANI M | 2011 | Journal of the American Ceramic Society2011，94，3： | 1 |
| 6 | Production of high protein quality noodles using wheat flour fortified with different protein products from lupine显示文摘 | MAHMOUD E A M NASSEF S L AMANY BASUNY M M | 2012 | Annals of Agricultural Science2012，57，2： | 1 |
| 7 | Effect of blend levels on composite wheat doughs performance made from yam and cassava native starches and bread quality显示文摘 | Nindjin C Amani G N Sindic M | 2011 | Carbohydrate Polymers2011，86，4： | 1 |
| 8 | Correlation of serum lipoprotein ratios with insulin resistance in infertile women with polycystic ovarian syndrome:a case control study显示文摘 | Ghaffarzad A Amani R Mehrzad Sadaghiani M | 2016 | Int J Fertil Steril2016，10，1： | 1 |
| 9 | Canopy temperature depression associated with yield of irrigated spring wheat cultivars in a hot climate显示文摘 | Amani Iischer R A Eynolds M P | 1996 | J Agron Crop Sci1996，176，2： | 1 |
| 10 | Optimization of the Prodution of biosurfactant from Iranian indigeous bacteria for the reduction of surface tension and enhanced oil recovery显示文摘 | AMANI H HAGHIGHI M SARRAFZADEH M H | 2011 | Petroleum Science and Technology2011，29，3： | 1 |
| 11 | Pre-treatment effect of different doses of soy isoflavones on spatial learning and memory in an ovariectomized animal model of Alzheimer's disease显示文摘 | Sarkaki A Amani R Badavi M | | 0，，08： | 1 |
| 12 | Enhancement of Decolorization Rate and COD Removal from Dyes Containing Wastewater by the Addition of Hydrogen Peroxide Under Solar Photocatalytic Oxida- tion 显示文摘 | Ghaly Montaser Y Farah Joseph Y Fathy Amany M | 2007 | Desalination2007，217，13： | 1 |
| 13 | Study of syntrophic anaerobic digestion of volatile fatty acids using enriched cultures at mesophilic conditions显示文摘 | AMANI T NOSRATI M MOUSAVI S M | | 0，，： | 1 |
| 14 | Effect of ovariectomy on reference memory version of Morris water maze in young adult rats显示文摘 | Sarkaki A Amani R Badavi M | 2008 | Iran Biomed J2008，12，2： | 1 |
| 15 | Alli- cin from garlic inhibits the biofilm formation and urease activ- ity of Proteus mirabilis in vitro 显示文摘 | RANJBAR- OMID M ARZANLOU M AMANI M | 2015 | FEMS Microbiol Lett2015，362，9： | 1 |
| 16 | Detection of triacetone triperoxide (TATP) using a thermodynamic based gas senor显示文摘 | AMANI M CHU Y WATERMAN K L | 2012 | Sensors and Actuators B-Chemical2012，162，1： | 1 |
| 17 | Canopy temperature depression associated with yield of irrigated spring wheat cultivation a hot climate显示文摘 | AMANI I FISCHER R A REYNOLDS M P | 1996 | Journal of Agronomy and Crop Science1996，176，2： | 1 |
| 18 | Culti-objective Simu-lated Annealing Algorithm for Partner Selection in Virtual Enterpri-ses显示文摘 | Hisham M Abdelsalam Amany M Mohamed | 2013 | Computational intelligence2013，427，： | 1 |
| 19 | Effect of HEMADO on Level of CKMB and LDH Enzymes after Ischemia/Reperfusion In- jury in Isolated Rat Heart 显示文摘 | Amani M Jeddi S Ahmadiasl N | 2013 | Bioimpacts2013，3，2： | 1 |
| 20 | Optimiza- tion of the production of biosurfactant from Iranian indigenous bacteria for the reduction of surface tension and enhanced oil re- covery显示文摘 | AMANI H HAGHIGHI M SARRAFZADEH M H | 2011 | Petrol Sci Technol2011，29，30： | 1 |
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