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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | 通过DAVID电离室信号剖面的迭代解卷积加强IMRT应用固定检测的准确性显示文摘 | H K Looe D Harder A Ruhmann K C Willborn B Poppe | 2010 | 医学生物学物理2010，，： | 1 |
| 3 | Modulation of learning and anxiety by corticotropin-releasing factor (CRF) and stress: Differential roles of CRF receptor 1 and 2 显示文摘 | RUHMANN A | 1999 | Journal of Neuroscience1999，19，12： | 1 |
| 4 | The mechanical properties of cancellous bone in the proximal tibia of ovariectomized rats显示文摘 | HOGAN H A RUHMANN S P SAMPSON H W | 2000 | J Bone Miner Res2000，15，2： | 1 |
| 5 | Thermochromic polymers- Function by design显示文摘 | Seeboth A Lotzsch D Ruhmann R | 2014 | Chemical Reviews2014，114，5： | 1 |
| 6 | Enhaneed aeeuracy of the permanent surveillance of IMRT deliveries by iterative deeonvolu- tion of DAVID chamber signal profiles显示文摘 | H K Looe D Harder A Ruhmann | 2010 | Phys Med Biol2010，55，： | 1 |
| 7 | Thermotropic and thermochromic polymer based materials for adaptive solar control显示文摘 | Seeboth A Ruhmann R Muhling O | 2010 | Materials2010，3，12： | 1 |
| 8 | Modulation of learning and anxiety by corticotropin-releasing factor (CRF) and stress: differential roles of CRF receptors 1 and 2显示文摘 | Radulovic J Ruhmann A Liepold T Spiess J | 1999 | J Neurosci1999，19，： | 1 |
| 9 | The effect of a carben- fiber couch on the depth-dose curves and transmission properties for megavoltage photon beams 显示文摘 | Poppe B Chofor N Ruhmann A et ai | 2007 | Strahlenther Onkol2007，183，： | 1 |
| 10 | The effect of a carbon-fiber couch on the depth-dose curves and transmission properties for megavoltage photon beams显示文摘 | Poppe B Chofor N Ruhmann A | 2007 | Strahlenther Onkol2007，183，1： | 1 |
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