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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 3 | Expression,purification and immunocharacteristics of recombination UreB protein of H.pylori显示文摘INTRODUCTIONHelicobacter pylori (H . pylori) is associated with the development of chronic gastritis ,peptic ulcer and gastric cancer and gastric MALT lymphoma[1-9],H .pylori has many antigens ,including urease ,heat shock protein and vacuolating cytotoxin and so on ,and urease is an important factor in the colinization of the gastric mucosa and suspected to cause damage to the gastric mucosa[10-14].At the same time ,urdase is also one of the important protective antigens . | Chao Wu~1 Quan Ming Zou~1 Hong Guo~2 Xiao Peng Yuan~1 Wei Jun Zhang~1 Dong Shui Lu~1 Xu Hu Mao~1 ~1Department of Clinical Microbiology,Third Military Medical University,Chongqing 400038,China ~2Department of Gastroenterology,Xinqiao Hospital,Third Military Medical University,Chongqing 40003?,ChinaDr.Chao Wu graduated from Third Military Medical University as a postgraduate in 2000,now a lecturer,specialized in diagnosis,prevention and therapy of Helicobacter pylori infection,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 5 |
| 4 | Changes in nitrogen and phosphorus status under general and waste irrigation显示文摘 | Shrivastava V S Dusane A B 2000 | 2000 | Pollution Research2000，19，： | 1 |
| 5 | Recent topics in photoelectrochemistry:achievements and future prospects显示文摘 | Tryk D A Fujishima A Honda K 2000 | 2000 | Electrochim Acta2000，45，： | 1 |
| 6 | Epidemiological evidence of the effects of ultrafine particle exposure显示文摘 | Wichmann H E Peters A 2000 | 2000 | The Royal Society2000，358，： | 1 |
| 7 | New perspectives on nitrogen-fixing microorganisms in tropical and subtropical oceans显示文摘 | Zehr J P E J Carpenter and T A Villareal 2000 | 2000 | Trends in Microbiology2000，8，2： | 1 |
| 8 | Anoxia pretreatment protects soybean cells against H202-induced cell death:possible involvement of peroxidases and of alternative oxidase显示文摘 | Amor Y Chevion M Levine A 2000 | 2000 | FEES Lett2000，477，： | 1 |
| 9 | Three-fixation combined with bone 显示文摘 | Goslings J C Schulrman H dimensional dynamic external grafting for Kienbock's disease Upper Extremmity Surgery 2000 A Broekhcizen A H | 2000 | Techniques in Hand and2000，4，2： | 1 |
| 10 | Global and regional land cover characterization born satellite data: an introduction to the special issue显示文摘 | DeFries R S Belward A S 2000 | 2000 | International Journal of Remote Sensing2000，21，67： | 1 |
| 11 | Soil respiration and global carbon cycle显示文摘 | Schlesinger W H Andrews J A 2000 | 2000 | Biogeochemistry2000，48，： | 1 |
| 12 | Solid leaehate recirculation显示文摘 | GABR M A HOSSAIN waste settlement with Geotechnicai News 2000 M S BARLAZ M A | 2000 | 18(2): 50 - 552000，18，2： | 1 |
| 13 | Relation- ships between fine root dynamics and nitrogen availability in Michigan northern hardwood forests 显示文摘 | BURTON A J PREGITZER KS HENDRICK RL 2000 | 2000 | Oecologia2000，125，3： | 1 |
| 14 | Photodegradation of metolachor: Isolation,identification, and quantification of monochloroacetic acid 显示文摘 | Mabury S A 2000 | 2000 | J Agric Food Chem2000，48，： | 1 |
| 15 | Soil effects of transgenic agriculture:biological processes and ecological consequences显示文摘 | McGregor A N Turner M A 2000 | 2000 | NZ Soil News2000，48，6： | 1 |
| 16 | Landscape structure analysis of Kansas at three scales显示文摘 | Griffith J A Martinko E A Price K P 2000 | 2000 | Landscape and Urban Planning2000，52，： | 1 |
| 17 | Control of abscisic acid synthesis 显示文摘 | Taylor IB Burhidge A Thompson AJ 2000 | 2000 | J Exp Bot2000，51，： | 1 |
| 18 | Detection of Tricholoma matsutake by specific ITS primers显示文摘 | Kikuchi K Matsushita N Guerin-Laguette A 2000 | 2000 | Mycol Res2000，104，12： | 1 |
| 19 | The relationship between residents' attitudes toward tourism and tourism development options显示文摘 | Andereck K L Vogt C A(2000) | 2000 | Journal of Travel Research2000，2736，： | 1 |
| 20 | A simple model for batch activated sludge process显示文摘 | Kazmi A A Furumai H 2000 | 2000 | Water Sci Technol2000，42，34： | 1 |
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