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Genetic Relationships among Prunus mume var. pendula Using AFLP Markers

查看全文 作  者:Ming Jun1* Zhang Qixiang2 Ru Guangxin3 Mao Qingshan4 Yan Xiaolan4 Lan Yanping5 1Department of Horticulture, Hubei Agricultural College, Jingzhou 434025, P. R. China 2College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P. R. China 3Department of Forestry, Henan Agricultural University, Zhengzhou 450000, P. R. China 4The Research Center of China Mei Flowers, Wuhan 430000, P. R. China 5Institute of Plant Nutrition and Resource, Beijing Academy of Agriculture and Forestry, Beijing 100091, P. R. China 高影响力作者 出  处:《Forestry Studies in China》索引2003年第5卷第4期,共5页高影响力期刊 基  金:the Natural Science Foundation of Hubei Province; P. R. China (Grant No. 2001ABB118) 摘  要:Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form. 关 键 词:PRUNUS mume Sieb. et Zucc., amplified FRAGMENT length POLYMORPHISM (AFLP), PRUNUS murne VAR. pendula (Pendulous MCI Group), genetic relationship
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