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| 1 | Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants显示文摘Green fl uorescent protein(GFP)has been widely used for monitoring gene expression and protein localization in diverse organisms.However,highly sensitive imaging equipment,like fl uorescence microscope,is usually required for the visualization of GFP,limitings its application to fi xed locations in samples.A reporter that can be visualized in realtime regardless the shape,size and location of the target samples will increase the fl exibility and ef fi ciency of research work.Here,we report the application of a GFP-like protein,called eYGFPuv,in both transient expression and stable transformation,in two herbaceous plant species(Arabidopsis and tobacco)and two woody plant species(poplar and citrus).We observed bright fl uorescence under UV light in all of the four plant species without any effects on plant growth or development.eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues.With a focus on in vitro transformation,we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identi fi ed visually during the callus stage and the shoot stage,enabling early and ef fi cient selection of transformants.Furthermore,whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants.In addition,our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics.This work demonstrates the feasibility of utilizing 1x eYGFPuv in studies of gene expression and plant transformation in diverse plants. | Guoliang Yuan Haiwei Lu Dan Tang Md Mahmudul Hassan Yi Li Jin-Gui Chen Gerald A.Tuskan Xiaohan Yang | 2021 | Horticulture Research2021，8，1： | 1 |
| 2 | Simple method for transformation and gene editing in medicinal plants显示文摘Gene-editing technologies have ushered in a significant advancement in plant genetics research and molecular breeding.However,a critical challenge hindering the widespread adoption of these technologies is the efficient delivery of gene-editing tools.The predominant methods for introducing these tools into plants typically involve Agrobacterium tumefaciens-mediated transformation or particle bombardment(Mao et al.,2019).Unfortunately,these traditional gene delivery methods require delicate and timeconsuming tissue culture procedures and show limited success,especially in medicinal and other less studied plants.A recent breakthrough is the development of the Cut-Dip-Budding(CDB)gene delivery system.The CDB system is highly effective for plants with root-suckering capabilities.It allows the delivery of transgenes and gene-editing tools into plants through hairy root induction followed by shoot regeneration from transformed hairy roots,bypassing the need for tissue culture processes(Cao et al.,2023). | Xuesong Cao Hongtao Xie Minglei Song Lianghui Zhao Hailiang Liu Guofu Li Jian‐Kang Zhu | 2024 | Journal of Integrative Plant Biology2024，66，1： | 0 |
| 3 | CRISPR-mediated acceleration of wheat improvement:advances and perspectives显示文摘Common wheat(Triticum aestivum)is one of the most widely cultivated and consumed crops globally.In the face of limited arable land and climate changes,it is a great challenge to maintain current and increase future wheat production.Enhancing agronomic traits in wheat by introducing mutations across all three homoeologous copies of each gene has proven to be a difficult task due to its large genome with high repetition.However,clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associ-ated nuclease(Cas)genome editing technologies offer a powerful means of precisely manipulating the genomes of crop species,thereby opening up new possibilities for biotechnology and breeding.In this review,we first focus on the development and optimization of the current CRISPR-based genome editing tools in wheat,emphasizing recent breakthroughs in precise and multiplex genome editing.We then describe the general procedure of wheat genome editing and highlight different methods to deliver the genome editing reagents into wheat cells.Furthermore,we summarize the recent applications and ad-vancements of CRISPR/Cas technologies for wheat improvement.Lastly,we discuss the remaining chal-lenges specific to wheat genome editing and its future prospects. | Ximeng Zhou Yidi Zhao Pei Ni Zhongfu Ni Qixin Sun Yuan Zong | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
| 4 | 植物中甜菜色素的研究进展显示文摘甜菜色素是一类水溶性含氮色素,与花青素在功能和器官分布上具有很多相似性。文章综述了国内外在甜菜色素研究中的相关进展,以期为更好地理解甜菜色素的生物合成机制及进一步开发利用提供参考依据。甜菜色素主要存在于石竹目植物中,分为甜菜红素和甜菜黄素,它不仅具有吸引昆虫授粉的作用,还可作为一种重要的渗透调节物质和非酶促抗氧化剂帮助植物抵抗逆境,维持植物体内正常的生理活动。甜菜色素生物合成途径独特,具有重要的医疗保健价值,目前已被广泛应用于食品、药物和化妆品中。文章从理化性质、合成代谢调控、生物学功能和应用价值等方面对甜菜色素进行了介绍,重点阐述了近年来在甜菜色素和花青素的关系以及光对甜菜色素生物合成影响方面的研究进展。 | 黄晨 张威 任红旭 | 2023 | 西北植物学报2023，43，12： | 0 |
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