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| 1 | 傅里叶叠层显微成像技术:理论、发展和应用显示文摘高分辨率是光学显微技术发展至今不断追求的目标之一。然而随着当前显微镜系统功能与性能的不断革新,高分辨率与大视场难以同时兼顾的问题日益突出,这个问题极大地限制了其在许多领域的应用。傅里叶叠层显微成像技术(FPM)是近年来发展出的一种新型计算成像技术,其能通过同时恢复强度和相位分布来提供宽域高分辨率的成像能力。FPM虽然是在2013年才被提出,但是由于其融合了大视场、高分辨率、定量相位成像等诸多优点,近年来已经在光学显微、生物医学、生命科学等领域获得了大量研究和广泛关注。从基本原理、实验系统与成像模式、系统与算法的改进方法等几个方面对FPM的研究现状、应用领域和最新进展进行了综述,并讨论了现存的一些关键问题以及今后可能的研究方向。 | 孙佳嵩 张玉珍 陈钱 左超 | 2016 | 光学学报2016，36，10： | 31 |
| 2 | Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens显示文摘A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view(FOV).We report a subvoxel light-sheet microscopy(SLSM)method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution.A nonaxial,continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional(3-D)nanoscale shifts encoded.Then,by adopting a subvoxel-resolving procedure,the SLSM method models these low-resolution,cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample.This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times,with high acquisition speeds of gigavoxels per minute.By fast reconstruction of 3-D cultured cells,intact organs,and live embryos,SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue(>100 mm3)and observing single cell(∼1-μm resolution).It also eliminates the need of complicated mechanical stitching or modulated illumination,using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput,high-resolution 3-D microscopy,which could be tailored for a wide range of biomedical applications in pathology,histology,neuroscience,etc. | Peng Fei Jun Nie Juhyun Lee Yichen Ding Shuoran Li Hao Zhang Masaya Hagiwara Tingting Yu Tatiana Segura Chih-Ming Ho Dan Zhu Tzung KHsiaic | 2019 | Advanced Photonics2019，1，1： | 6 |
| 3 | High spatial and temporal resolution synthetic aperture phase microscopy显示文摘A new optical microscopy technique,termed high spatial and temporal resolution synthetic aperture phase microscopy(HISTR-SAPM),is proposed to improve the lateral resolution of wide-field coherent imaging.Under plane wave illumination,the resolution is increased by twofold to around 260 nm,while achieving millisecond-level temporal resolution.In HISTR-SAPM,digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability.An off-axis interferometer is used to measure the sample scattered complex fields,which are then processed to reconstruct high-resolution phase images.Using HISTR-SAPM,we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap(i.e.,a full pitch of 330 nm).As the reconstruction averages out laser speckle noise while maintaining high temporal resolution,HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells,such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells.We envision that HISTR-SAPM will broadly benefit research in material science and biology. | Cheng Zheng Di Jin Yanping He Hongtao Lin Juejun Hu Zahid Yaqoo Peter T.C.So Renjie Zhou | 2020 | Advanced Photonics2020，2，6： | 5 |
| 4 | Frequency-domain diagonal extension imaging显示文摘The pixel size of a charge-coupled device(CCD)camera plays a major role in the image resolution,and the square pixels are attributed to the physical anisotropy of the sampling frequency.We synthesize the high sampling frequency directions from multiple frames acquired with different angles to enhance the resolution by 1.4×over conventional CCD orthogonal sampling.To directly demonstrate the improvement of frequency-domain diagonal extension(FDDE)microscopy,lens-free microscopy is used,as its resolution is dominantly determined by the pixel size.We demonstrate the resolution enhancement with a mouse skin histological specimen and a clinical blood smear sample.Further,FDDE is extended to lens-based photography with an ISO 12233 resolution target.This method paves a new way for enhancing the image resolution for a variety of imaging techniques in which the resolution is primarily limited by the sampling pixel size,for example,microscopy,photography,and spectroscopy. | Shan Jiang Meiling Guan Jiamin Wu Guocheng Fang Xinzhu Xu Dayong Jin Zhen Liu Kebin Shi Fan Bai Shu Wang Peng Xi | 2020 | Advanced Photonics2020，2，3： | 3 |
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