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| 1 | Repair calvarial defect of osteoporotic rats by berberine functionalized porous calcium phosphate scaffold显示文摘In this article,we propose a simple scheme of using berberine(BBR)to modify porous calcium phosphate ceramics(named PCPC).These BBR molecules regulate the crystallization of hydroxyapatite nanorods on PCPC.We found that these nanorods and the adsorbed BBR changed the interface micro-environment of PCPC by SEM images.The microenvironment of PCPC surface is essential for promoting BMSCs’proliferation and differentiation.These results demonstrated that PCPC/BBR markedly improved the bone regeneration of osteoporosis rats.Moreover,PCPC/BBR had significantly increased the expression levels of ALP,osteocalcin and bone morphogenetic protein2 and RUNX2 in BMSCs originated from osteoporosis rats. | Dahao Wang Peng Zhang Xifan Mei Zhenhua Chen | 2021 | Regenerative Biomaterials2021，8，3： | 2 |
| 2 | 茶多酚对骨组织细胞的影响及其在组织工程中的应用进展显示文摘骨组织在维持机体运动与健康方面具有重要的作用,研究骨组织细胞的生物学功能及其在组织工程中的应用对临床预防及治疗骨科相关疾病具有重要意义。茶多酚是茶叶的主要成分,其具有天然的抗氧化性、清除自由基活性和抗炎作用。研究表明,茶多酚主要通过增强成骨细胞形成和减少破骨细胞的发生来促进骨重建过程;此外,茶多酚还可作为人造骨材料的表面修饰分子,在骨组织工程领域广泛应用。综述了茶多酚在骨组织细胞中的调控作用及其在组织工程领域的应用,并对相关的研究内容进行回顾和展望,以期为开展后续的研究工作提供参考及理论基础。 | 曹振 潘光玉 王丽萍 郭勇 | 2020 | 国际生物医学工程杂志2020，43，3： | 1 |
| 3 | 茶多酚对胫骨骨折大鼠愈合及BMP2/Smad1/Runx2通路的影响显示文摘目的探究茶多酚(tea polyphenols, TP)对胫骨骨折大鼠的愈合作用,并初步探究其作用机制。方法将大鼠分为假手术组(Sham),模型组(Model),TP低、中、高剂量组(TP-L、TP-M、TP-H),阳性组(骨肽片组,Ossotide组),每组12只大鼠,X射线观察大鼠胫骨骨折愈合情况,并扫描离体胫骨检测骨矿物质密度值(BMD);HE染色观察胫骨组织形态学变化;酶动力学法检测血清碱性磷酸酶(alkaline phosphatase, ALP)水平;氮蓝四唑光还原法检测超氧化物歧化酶(superoxide dismutase, SOD)水平,硫代巴比妥酸法检测丙二醛(malondialdehyde, MDA)水平;蛋白免疫印迹法检测骨形态发生蛋白-2(BMP2)、磷酸化-Sma-Mad蛋白1(p-Smad1)、Sma-Mad蛋白1(Smad1)、Runt相关转录因子2(Runx2)蛋白表达情况。结果与Control组相比,Model组胫骨影像学愈合评分降低,BMD降低,胫骨出现较多纤维组织及软骨组织,形态学愈合评分降低,血清ALP水平降低,MDA升高,SOD降低,BMP2、p-Smad1、Runx2蛋白表达降低,差异显著(P<0.05);与Model组相比,TP各组、Ossotide组胫骨影像学愈合评分升高,BMD升高;纤维组织逐渐被骨组织替代,形态学愈合评分升高;血清ALP水平升高,MDA降低,SOD升高,BMP2、p-Smad1、Runx2蛋白表达升高,具有剂量依赖性,差异显著(P<0.05)。结论 TP可促进胫骨骨折大鼠骨折愈合,其机制可能与降低组织氧化应激损伤,激活BMP2/Smad1/Runx2通路有关。 | 刘立民 索南昂秀 | 2021 | 沈阳药科大学学报2021，38，5： | 1 |
| 4 | 茶多酚对牛乳过敏原α_(s1)-酪蛋白构象和抗原性的影响显示文摘为探明茶多酚对α_(s1)-酪蛋白构象和抗原性的影响,选用茶多酚中活性较强的表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)和表没食子儿茶素(epigallocatechin,EGC)接枝α_(s1)-酪蛋白。通过荧光光谱、同步荧光光谱和圆二色谱研究α_(s1)-酪蛋白构象变化,通过间接竞争酶联免疫方法、免疫印迹实验分析α_(s1)-酪蛋白抗原性变化。结果表明:EGC、EGCG均对α_(s1)-酪蛋白内部荧光有猝灭作用,对α_(s1)-酪蛋白的酪氨酸(tyrosine,Tyr)和色氨酸(tryptophan,Trp)残基均有影响,α_(s1)-酪蛋白的α-螺旋和β-折叠含量略有增加,无规卷曲含量略有降低;α_(s1)-酪蛋白构象的变化导致其抗原性显著降低,EGCG对α_(s1)-酪蛋白构象和抗原性的影响显著强于EGC。 | 郭红蕾 周胜云 丛艳君 闫文杰 | 2022 | 乳业科学与技术2022，45，4： | 0 |
| 5 | Preparation of EGCG decorated,injectable extracellular vesicles for cartilage repair in rat arthritis显示文摘Arthritis is a kind of chronic inflammatory autoimmune disease,which can destroy joint cartilage and bone,leading to joint pain,joint swelling,and limited mobility.Traditional therapies have many side effects or focus too much on anti-inflammation while neglecting joint repair.In this experiment,we combined Epigallocatechin gallate(EGCG)with extracellular vesicles derived from macrophages to treat rheumatoid arthritis.Sustained-release resulted in a significant decrease in chondrocyte expression of hypoxia-inducible factor 1-alpha,a decrease in apoptosis-related proteins Cytochrome C,Caspase-3,Caspase-9,and Bax.Molecular biological analysis showed that extracellular vesicles-encapsulated EGCG(EVs-EGCG)more significantly upregulated type II collagen expression by about 1.8-fold than EGCG alone,which was more beneficial for arthritis repair.Animal experiments revealed that these EGCG-coated extracellular vesicles significantly reduced swelling,decreased synovial hyperplasia,repaired cartilage,and attenuated arthritis-related pathology scores in arthritic rats.Measurement data showed that EVs-EGCG treatment reduced joint swelling by approximately 39.5%in rheumatoid rats.In vitro studies have shown that this EVs-EGCG can increase the expression of cartilage type II collagen and reduce apoptosis of chondrocytes.Moreover,it was demonstrated in vivo experiments to reduce cartilage destruction in rheumatoid arthritis rats,providing a solution for the treatment of rheumatoid arthritis. | Changwei Song Shibo Xu Linna Chang Xingjun Zhao Xifan Mei Xiuli Ren Zhenhua Chen | 2021 | Regenerative Biomaterials2021，8，6： | 0 |
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