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| 1 | Multiplexed intracellular detection based on dual-excitation/dualemission upconversion nanoprobes显示文摘Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously.Herein,we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles(UCNP)loaded with two dyes for sensitization and quenching of the upconversion luminescence(UCL),respectively.Based on the two independent energy transfer processes of near-infrared(NIR)dye IR845 to UCNP and UCNP to visible dye PAPS-Zn,CIO-and Zn2+were simultaneously detected with a limit of detection(LOD)of41.4 and 10.5 nM,respectively.By tilizing a purpose built 830/980 nm dual-laser confocal microscope,both intrinsic and exogenous CIO and Zn2+in live MCF-7 cells have been accurately quantified.Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells.Through modulation of NIR dye and visible dye with other reactive groups,the nanoprobes can be extended to analyze various intraellular species,which provides a promising tool to study the biological activities in live cells and diagnose diseases. | Jianxi Ke Shan Lu Zhuo Li Xiaoying Shang Xingjun Li Renfu Li Datao Tu Zhuo Chen Xueyuan Chen | 2020 | Nano Research2020，13，7： | 2 |
| 2 | Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time显示文摘Controllably and efficaciously localized CRISPR/Cas9 plasmids transfection plays an essential role in genetic editing associated with various key human diseases.We employed near-infrared(NIR)light-responsive CRISPR/Cas9 plasmids delivery via a charge-reversal nanovector to achieve highly efficient and site-specific gene editing.The nanovector with abundant positive charges was fabricated on the basis of an ultraviolet-sensitive conjugated polyelectrolyte coated on an upconversion nanomaterial(UCNP-UVP-P),which can convert into negative charges upon 980 nm light irradiation.Using the as-prepared nanovector,we demonstrated the plasmids could be efficiency transfected into tumor cells(~63%±4%)in a time-contolled manner,and that functional CRISPR/Cas9 proteins could be successfully expressed in a selected NIR-irradiated region.Particularly,this strategy was successfully applied to the delivery of CRISPR/Cas9 gene to tumor cells in vivo,inducing high efficiency editing of the target gene PLK-1 under photolrradiation.Therefore,this precisely controlled gene regulation strategy has the potential to serve as a new paradigm for gene engineering in complex biological systems. | Yunxia Wu Judun Zheng Qin Zeng Tao Zhang Da Xing | 2020 | Nano Research2020，13，9： | 2 |
| 3 | 基于透明微针拉曼原位检测H2O_(2)显示文摘在聚甲基丙烯酸甲酯-微针(PMMA-MNs)阵列上修饰聚多巴胺(PDA)层,通过离心技术将金纳米粒子(AuNPs)组装在MNs表面,构建纳米金@聚多巴胺@聚甲基丙烯酸甲酯微针阵列(Au@PDA@MNs).Au@PDA@MNs有强表面增强拉曼散射(SERS)效应,以罗丹明6G(R6G)作为拉曼探针,在10^(-4)~10^(-8) mol·L^(-1)的范围内有良好的线性响应,检测限(LOD)可达3.1×10^(-10) mol·L^(-1).为了检测过氧化氢(H2O_(2)),4-巯基苯硼酸(4-MPBA)被修饰在Au@PDA@MNs上以构建功能化的MNs.利用4-MPBA分子氧化生成4-羟基硫代苯酚(4-HTP)和MNs的透光性,基于1000 cm^(-1)和1068 cm^(-1)处拉曼强度比(I1000/I1068)的变化,完成了H2O_(2)的拉曼定量检测,I1000/I1068的变化与H2O_(2)浓度成定量关系.线性响应范围为10~2000μmol·L^(-1,)LOD为0.93μmol·L^(-1).MNs和SERS技术的结合为确定具有短半衰期和弱拉曼信号的疾病相关生物标志物开辟了一条新途径. | 彭程 王丰 杨海峰 | 2022 | 上海师范大学学报(自然科学版)2022，51，5： | 0 |
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