|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 高效液相色谱法测定酒醅中的乳酸含量显示文摘采用高效液相色谱法测定浓香型白酒酒醅中的乳酸含量。该方法采用浸提、冷冻过滤、减压蒸馏等前处理方式,通过安捷伦ZORBXSB-C18色谱柱(4.6 mm×250 mm,5μm)分离,以甲醇和磷酸二氢钾溶液(0.02 mol/L,p H2.1)作为流动相,流速0.2 mL/min,检测波长210 nm,柱温30℃,进样量10μL。在100~800 mg/L范围内呈线性相关,相关系数R2为0.99992,重现性RSD为1.11%~1.35%,加标回收率为98.88%~99.52%。该方法分析结果较好,差异不显著,能够满足定量分析要求。 | 张宝玉 李良 吴昊 刘莎 田鹏 张超 董孝元 | 2019 | 酿酒科技2019，，2： | 9 |
| 2 | In vitro cytocompatibility evaluation of poly(octamethylene citrate) monomers toward their use in orthopedic regenerative engineering显示文摘Citrate based polymer poly(octamethylene citrate)(POC)has shown promise when formulated into composite material containing up to 65 wt%hydroxylapatite(HA)for orthopedic applications.Despite significant research into POC,insufficient information about the biocompatibility of the monomers 1,8-Octanediol and Citrate used in its synthesis is available.Herein,we investigated the acute cytotoxicity,immune response,and long-term functionality of both monomers.Our results showed a cell-type dependent cytotoxicity of the two monomers:1,8-Octanediol induced less acute toxicity to 3T3 fibroblasts than Citrate while presenting comparable cytotoxicity to MG63 osteoblast-like cells;however,Citrate demonstrated enhanced compatibility with hMSCs compared to 1,8-Octanediol.The critical cytotoxic concentration values EC30 and EC50,standard for comparing cytotoxicity of chemicals,were also provided.Additionally,Citrate showed slower and less inhibitory effects on long-term hMSC cell proliferation compared with 1,8-Octanediol.Furthermore,osteogenic differentiation of hMSCs exposure to Citrate resulted in less inhibitory effect on alkaline phosphatase(ALP)production.Neither monomer triggered undesired pro-inflammatory responses.In combination with diffusion model analysis of monomer release from cylindrical implants,based on which the maximum concentration of monomers in contact with bone tissue was estimated to be 2.2104 mmol/L,far lower than the critical cytotoxic concentrations as well as the 1,8-Octanediol concentration(0.4 mg/mL or 2.7 mmol/L)affecting hMSCs differentiation,we provide strong evidence for the cytocompatibility of the two monomers degraded from citrate-based composites in the orthopedic setting. | Chuying Ma Ethan Gerhard Qiaoling Lin Silun Xia April Dawn Armstrong Jian Yang | 2018 | Bioactive Materials2018，3，1： | 2 |
| 3 | 副干酪乳杆菌在多巴胺改性聚丙烯纤维膜上形成生物膜及其发酵性能显示文摘利用多巴胺在材料表面的自发黏附特性,制备了多巴胺改性的聚丙烯纤维膜,研究多巴胺改性对副干酪乳杆菌(Lactobacillus paracaise)在聚丙烯纤维膜上形成生物膜的影响及L.paracasei生物膜的发酵性能。利用扫描电子显微镜、红外光谱仪及接触角仪对多巴胺改性聚丙烯纤维膜表面形成的L.paracasei生物膜进行表征,并对L.paracasei生物膜的抗逆性与发酵能力进行研究。结果表明,多巴胺改性对聚丙烯纤维膜表面L.paracasei生物膜形成具有显著的促进作用,多巴胺改性聚丙烯纤维膜表面的生物膜具有极佳的抗逆性能,更耐受高温和酸碱环境,用于生物膜反应器中发酵生产的L-乳酸纯度维持在99%以上,同时反应器基本没有发酵延滞期,有效提高了生物发酵效率。 | 赵子舒 李吉年 郭谦 叶翔宇 胡梦欣 | 2017 | 食品科学2017，38，14： | 2 |
| 4 | 乳酸对成骨样细胞成骨表型及碱性磷酸酶基因表达的影响研究显示文摘目的明确聚乳酸降解终产物乳酸对细胞成骨表型和基因表达的影响机理,为聚乳酸支架的精确设计提供参考意义。方法配制乳酸浓度为8、16、22、27 mmol/L的培养基,将不含乳酸培养基作为对照组,检测培养基的pH和渗透压。利用培养基培养大鼠成骨样细胞Ros17/2.8,观察细胞形态,测定细胞Ⅰ型胶原、碱性磷酸酶(alkaline phosphatase,ALP)活性和骨钙素含量,盐酸四环素染色钙结节并定量分析。采用实时定量PCR法检测细胞ALP mRNA表达。结果与对照组相比,随着乳酸浓度增加,培养基pH下降,渗透压升高,成骨样细胞形态改变,数量减少。低浓度乳酸组(8、16 mmol/L)的Ⅰ型胶原量都显著高于对照组,其余组与对照组相比无显著差异。含乳酸组的细胞ALP活性都低于对照组,并且乳酸浓度越高,细胞ALP活性越低。含乳酸组的骨钙素量与对照组之间没有显著差异。随着乳酸浓度增加,含乳酸组细胞所形成的钙结节阳性面积减少,都低于对照组。低浓度乳酸组(8、16 mmol/L)的细胞ALP mRNA表达量都低于对照组。结论聚乳酸降解终产物乳酸可抑制细胞ALP mRNA的表达,降低ALP活性。随着乳酸浓度增加,乳酸抑制作用增强,最终降低细胞的成骨矿化能力。此外,乳酸不会抑制细胞分泌I型胶原和骨钙素。 | 刘艳军 徐永平 李淑英 罗祥林 陈元维 | 2023 | 中国骨质疏松杂志2023，29，8： | 0 |
| 5 | From Chinese Science Bulletin to Science Bulletin: celebrate the coming 50th birthday显示文摘As the only multidisciplinary journal supervised by Chinese Academy of Sciences and the National Natural Science Foundation of China,Science Bulletin will usher in its50th anniversary in 2016.A fifty-year history for a journal is always remarkable.However,the more important reason to celebrate besides the great age is:the fifty-year old journal continues to be vigorous and influential. | Xuming Jia Rui An Xiao-Ya Chen | 2015 | Science Bulletin2015，60，24： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员