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| 1 | Control of Cell Wall Extensibility during Pollen Tube Growth显示文摘在这评论,当时,我们探讨成长尖端的花粉试管怎么完成它延伸的快速的率的问题 maintainingan 未经触动的房间墙。尽管 turgor 是必要的让生长发生,本地扩大率被本地 changesin 控制顶端的墙的粘性。我们集中于是思维 tobe 的几不同结构和内在的过程肌动朊细胞骨架,钙和质子生理学的主要参加者包括 exocytosis,组织和活动,和细胞的 energetics。我们认为肌动朊细胞骨架,特别地顶端的外皮的肌动朊穗,指导泡的 theflow 到顶端的领域,在他们与血浆膜熔化并且作出贡献的地方,他们到 expandingcell 的内容围。当时 pH 坡度由沿着 clearzone 的方面位于血浆膜的 proton-ATPase 产生了可以在穗调整快速的肌动朊周转和新聚合,集中尖端的钙坡度偏爱 secretiontowards 极的轴。显示出新墙材料的那 exocytosis 的最近的数据先于并且预言过程 ofcell 延伸为最新分泌的果胶的摄取贡献减少 inapical 的想法提供支持墙粘性并且到房间扩大。另外的主要因素将是由果胶的酸的钙的酶 pectinmethyl-esterase,和 chelation 的本地化和活动。最后,我们为反应的氧 speciesin 承认一个角色墙 viscosity.Key 词的控制: | Peter K. Hepler Caleb M. Rounds Lawrence J. Winship | 2013 | Molecular Plant2013,6,4: | 9 |
| 2 | Higher-Ordered Actin Structures Remodeled by Arabidopsis ACTIN-DEPOLYMERIZING FACTOR5 Are Important for Pollen Germination and Pollen Tube Growth显示文摘肌动朊细胞骨架的动力学为花粉萌芽和花粉试管生长是必要的。使解聚肌动朊的因素(ADF ) 典型地由切断 / 使解聚肌动朊细丝贡献肌动朊周转。最近,我们证明 Arabidopsis 亚纲 III ADF (ADF5 和 ADF9 ) 从保存 F-actin-depolymerizing 功能发展了 F-actin-bundling 功能。然而,很少对生理的功能,进化意义,和这些 neofunctionalized ADF 的肌动朊捆绑机制被知道。这里,我们报导 ? 引起的 ADF5 功能的那损失推迟了花粉萌芽,延迟的花粉试管生长,并且增加对由影响肌动朊捆的产生和维护的 latrunculin B (LatB ) 处理敏感。在生活房间的肌动朊细丝动力学的检查表明捆绑频率显著地在 adf5 花粉试管被减少,与它的生物化学的功能一致。进一步生物化学、基因的互补分析 ? 两个都表明了那 N-? 并且 ADF5 的 C 终端肌动朊绑定域为它的生理、生物化学的函数被要求。有趣地,当两个是不正常的肌动朊捆绑 ADF, ADF5,然而并非 ADF9 时,在成熟花粉起一个重要作用生理的活动。一起拿,我们的结果建议 ADF5 发展了捆绑肌动朊细丝的功能并且在花粉萌芽和花粉试管生长期间在肌动朊捆的形成,组织,和维护起一个重要作用。 | Jingen Zhu Qiong Nan Tao Qin Dong Qian Tonglin Mao Shunjie Yuan Xiaorong Wu Yue Niu Qifeng Bai Lizhe An Yun Xiang | 2017 | Molecular Plant2017,10,8: | 6 |
| 3 | Organelle trafficking, the cytoskeleton, and pollen tube growth显示文摘The pollen tube is fundamental for the reproduction of seed plants. Characteristically, it grows relatively quickly and uni‐directionally('polarized growth') to extend the male gametophyte to reach the female gametophyte. The pollen tube forms a channel through which the sperm cells move so that they can reach their targets in the ovule. To grow quickly and directionally, the pollen tube requires an intense movement of organelles and vesicles that allows the cell's contents to be distributed to sustain the growth rate. While the various organelles distribute more or less uniformly within the pollen tube, Golgi‐released secretory vesicles accumulate massively at the pollen tube apex, that is, the growing region. This intense movement of organelles and vesicles is dependent on the dynamics of the cytoskeleton,which reorganizes differentially in response to external signals and coordinates membrane trafficking with the growth rate of pollen tubes. | Giampiero Cai Luigi Parrotta Mauro Cresti | 2015 | Journal of Integrative Plant Biology2015,57,1: | 4 |
| 4 | Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning显示文摘极化的尖端生长是在许多优核质的一个基本细胞的过程。在这研究,我们检验了在 Arabidopsis 的在花粉尖端生长期间的到泡运输的肌动朊细胞骨架和它的关系的动态重构。我们发现从顶端的膜发源的肌动朊细丝形成由排列肌动朊与不同分布在外皮和内部细胞质的细丝绑的纵组成的专业化结构。用在联合与的基于肌动朊的药理学处理和基因异种捆牢(在 photobleaching 以后的荧光恢复) 在花粉试管的生长领域以内设想泡的运输的技术,我们证明外皮的肌动朊细丝便于尖端病房泡运输。我们也发现内部顶端的肌动朊细丝阻止泡的向后的运动,因此保证足够的泡在花粉试管尖端积累支持花粉试管的快速的生长。外皮、内部的顶端的肌动朊细丝的组合效果完美地在花粉试管尖端解释转换 V 锥形的泡分发模式的产生。当花粉试管转弯时,在面对的方面的顶端的肌动朊细丝经历 depolymerization 和聚合到使适应向新生长方向的顶端的肌动朊结构。重构的这肌动朊先于泡累积并且在试管形态学变化。因此,我们的学习在快速、方向性的花粉试管生长期间提供新卓见进在肌动朊动力学和泡运输之间的功能的关系。 | Xiaolu Qu Ruihui Zhang Meng Zhang Min Diao Yongbiao Xue Shanjin Huang | 2017 | Molecular Plant2017,10,7: | 3 |
| 5 | The pollen tube clear zone: Clues to the mechanism of polarized growth显示文摘Pollen tubes usually exhibit a prominent region at their apex called the 'clear zone' because it lacks light refracting amyloplasts. A robust, long clear zone often associates with fast growing pollen tubes, and thus serves as an indicator of pollen tube health. Nevertheless we do not understand how it arises or how it is maintained. Here we review the structure of the clear zone, and attempt to explain the factors that contribute to its formation. While amyloplasts and vacuolar elements are excluded from the clear zone,virtually all other organelles are present including secretory vesicles, mitochondria, Golgi dictyosomes, and the endoplas-mic reticulum(ER). Secretory vesicles aggregate into an inverted cone appressed against the apical plasma membrane.ER elements move nearly to the extreme apex, whereas mitochondria and Golgi dictyosomes move less far forward.The cortical actin fringe assumes a central position in the control of clear zone formation and maintenance, given its role in generating cytoplasmic streaming. Other likely factors include the tip-focused calcium gradient, the apical p H gradient, the in flux of water, and a host of signaling factors(small G-proteins). We think that the clear zone is an emergent property that depends on the interaction of several factors crucial for polarized growth. | Peter K.Hepler Lawrence J.Winship | 2015 | Journal of Integrative Plant Biology2015,57,1: | 3 |
| 6 | 微丝骨架调控植物特有生理活动的研究进展显示文摘微丝骨架参与了真核生物诸多重要的生理活动。真核生物的肌动蛋白均演化自同一祖先基因,在生化特性和调控机制上存在一定的相似性。动物和植物各自特异的生理活动和器官组成,动物和植物细胞中微丝骨架的存在形式、微丝结合蛋白种类及微丝动态调控机制等方面存在一定的差异。该文基于植物特有的生命活动和生理过程,重点归纳和概述了植物微丝骨架在部分植物特异生理活动中的作用机理的研究进展。 | 钱东 李盼盼 向云 | 2019 | 中国细胞生物学学报2019,41,3: | 2 |