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| 1 | Profilin Regulates Apical Actin Polymerization to Control Polarized Pollen Tube Growth显示文摘花粉试管生长在 flowering 植物复制期间是必要的步,其生长取决于动态顶端的肌动朊细丝的一张人口。顶端的肌动朊细丝被认为涉及泡熔化的规定并且在花粉试管指向。然而,在花粉试管调整顶端的肌动朊结构的构造的分子的机制仍然保持大部分不清楚。这里,我们在花粉试管在顶端的膜在肌动朊聚合的规定作为一个重要播放器识别 profilin。profilin 的 Downregulation 减少了在花粉试管的细丝状的肌动朊的数量和顶端的肌动朊细丝的导致的组织的破坏。肌动朊动力学的直接可视化表明在顶端的膜发源的肌动朊细丝的延伸在 profilin 异种花粉试管减少了。仅仅在有约束力的 poly-L-proline 是有缺点的变异的 profilin 部分在 profilin 异种花粉试管救肌动朊聚合缺点,尽管它充分救肌动朊周转显型。我们建议 profilin 在花粉试管尖端控制肌动朊结构的构造,大概由在顶端的膜赞成调停 formin 的肌动朊聚合。 | Xiaonan Liu Xiaolu Qu Yuxiang Jiang Ming Chang Ruihui Zhang Youjun Wu Ying Fu Shanjin Huang | 2015 | Molecular Plant2015，8，12： | 6 |
| 2 | Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning显示文摘极化的尖端生长是在许多优核质的一个基本细胞的过程。在这研究,我们检验了在 Arabidopsis 的在花粉尖端生长期间的到泡运输的肌动朊细胞骨架和它的关系的动态重构。我们发现从顶端的膜发源的肌动朊细丝形成由排列肌动朊与不同分布在外皮和内部细胞质的细丝绑的纵组成的专业化结构。用在联合与的基于肌动朊的药理学处理和基因异种捆牢(在 photobleaching 以后的荧光恢复) 在花粉试管的生长领域以内设想泡的运输的技术,我们证明外皮的肌动朊细丝便于尖端病房泡运输。我们也发现内部顶端的肌动朊细丝阻止泡的向后的运动,因此保证足够的泡在花粉试管尖端积累支持花粉试管的快速的生长。外皮、内部的顶端的肌动朊细丝的组合效果完美地在花粉试管尖端解释转换 V 锥形的泡分发模式的产生。当花粉试管转弯时,在面对的方面的顶端的肌动朊细丝经历 depolymerization 和聚合到使适应向新生长方向的顶端的肌动朊结构。重构的这肌动朊先于泡累积并且在试管形态学变化。因此,我们的学习在快速、方向性的花粉试管生长期间提供新卓见进在肌动朊动力学和泡运输之间的功能的关系。 | Xiaolu Qu Ruihui Zhang Meng Zhang Min Diao Yongbiao Xue Shanjin Huang | 2017 | Molecular Plant2017，10，7： | 3 |
| 3 | HSF1 facilitates the multistep process of lymphatic metastasis in bladder cancer via a novel PRMT5-WDR5-dependent transcriptional program显示文摘Background:Lymphatic metastasis has been associated with poor prognosis in bladder cancer patients with limited therapeutic options.Emerging evidence shows that heat shock factor 1(HSF1)drives diversified transcriptome to promote tumor growth and serves as a promising therapeutic target.However,the roles of HSF1 in lymphatic metastasis remain largely unknown.Herein,we aimed to illustrate the clinical roles and mechanisms of HSF1 in the lymphatic metastasis of bladder cancer and explore its therapeutic potential.Methods:We screened the most relevant gene to lymphatic metastasis among overexpressed heat shock factors(HSFs)and heat shock proteins(HSPs),and analyzed its clinical relevance in three cohorts.Functional in vitro and in vivo assays were performed in HSF1-silenced and-regained models.We also used Coimmunoprecipitation to identify the binding proteins of HSF1 and chromatin immunoprecipitation and dual-luciferase reporter assays to investigate the transcriptional program directed by HSF1.The pharmacological inhibitor of HSF1,KRIBB11,was evaluated in popliteal lymph node metastasis models and patientderived xenograft models of bladder cancer.Results:HSF1 expression was positively associated with lymphatic metastasis status,tumor stage,advanced grade,and poor prognosis of bladder cancer.Importantly,HSF1 enhanced the epithelial-mesenchymal transition(EMT)of cancer cells in primary tumor to initiate metastasis,proliferation of cancer cells in lymph nodes,and macrophages infiltration to facilitate multistep lymphatic metastasis.Mechanistically,HSF1 interacted with protein arginine methyltransferase 5(PRMT5)and jointly induced the monomethylation of histone H3 at arginine 2(H3R2me1)and symmetric dimethylation of histone H3 at arginine 2(H3R2me2s).This recruited the WD repeat domain 5(WDR5)/mixed-lineage leukemia(MLL)complex to increase the trimethylation of histone H3 at lysine 4(H3K4me3);resulting in upregulation of lymphoid enhancer-binding factor 1(LEF1),matrix metallopeptidase 9(MMP9),C-C motif chemokine ligand 20(CCL20),and E2F transcription factor 2(E2F2).Application of KRIBB11 significantly inhibited the lymphatic metastasis of bladder cancer with no significant toxicity.Conclusion:Our findings reveal a novel transcriptional program directed by the HSF1-PRMT5-WDR5 axis during the multistep process of lymphatic metastasis in bladder cancer.Targeting HSF1 could be a multipotent and promising therapeutic strategy for bladder cancer patients with lymphatic metastasis. | Ming Huang Wen Dong Ruihui Xie Jilin Wu Qiao Su Wuguo Li Kai Yao Yuelong Chen Qianghua Zhou Qiang Zhang Wenwen Li Liang Cheng Shengmeng Peng Siting Chen Jian Huang Xu Chen Tianxin Lin | 2022 | Cancer Communications2022，42，5： | 2 |
| 4 | Plant villins: Versatile actin regulatory proteins显示文摘Regulation of actin dynamics is a central theme in cell biology that is important for different aspects of cell physiology.Villin, a member of the villin/gelsolin/fragmin superfamily of proteins, is an important regulator of actin. Villins contain six gelsolin homology domains(G1–G6) and an extra headpiece domain. In contrast to their mammalian counterparts, plant villins are expressed widely, implying that plant villins play a more general role in regulating actin dynamics. Some plant villins have a de fined role in modifying actin dynamics in the pollen Invitube; most of their in vivo activities remain to be ascertained.Recently, our understanding of the functions and mechanisms of action for plant villins has progressed rapidly, primarily due to the advent of Arabidopsis thaliana genetic approaches and imaging capabilities that can visualize actin dynamics at the single filament level in vitro and in living plant cells. In this review,we focus on discussing the biochemical activities and modes of regulation of plant villins. Here, we present current understanding of the functions of plant villins. Finally, we highlight some of the key unanswered questions regarding the functions and regulation of plant villins for future research. | Shanjin Huang Xiaolu Qu Ruihui Zhang | 2015 | Journal of Integrative Plant Biology2015，57，1： | 2 |
| 5 | Premiums between Cross-listed Shares:Determinants and Assessment of Financial Reform Policy Effectiveness显示文摘This paper explores determinants of price premiums between A-share and American depositary receipts(ADR)or H-share and sheds light on policies using daily data from cross-listed companies from 2002-2020.Market sentiment and financial openness are critical in explaining both types of price premiums.Expected exchange rate changes significantly impact the A-share versus ADR premium but liquidity is essential for the A-share versus H-share premium.The introduction of Shanghai-Hong Kong Stock Connect in November 2014 has effectively increased the price discovery capacity of the A-share market,and investors were more adaptive to the RMB foreign exchange rate volatility after Chinese exchange rate system reform in 2015.The paper provides insights into future capital market reform in China. | Xuechun Zhang Ruihui Xu Xue Liu | 2022 | China & World Economy2022，30，3： | 0 |
| 6 | Edge atomic Fe sites decorated porous graphitic carbon as an efficient bifunctional oxygen catalyst for Zinc-air batteries显示文摘The development of advanced bifunctional oxygen electrocatalysts for oxygen reduction and evolution reactions(ORR and OER) is critical to the practical application of zinc-air batteries(ZABs). Herein, a silica-assisted method is reported to integrate numerous accessible edge Fe-Nx sites into porous graphitic carbon(named Fe-N-G) for achieving highly active and robust oxygen electrocatalysis. Silica facilitates the formation of edge Fe-Nx sites and dense graphitic domains in carbon by inhibiting iron aggregation.The purification process creates a well-developed mass transfer channel for Fe-N-G. Consequently,Fe-N-G delivers a half-wave potential of 0.859 V in ORR and an overpotential of 344 m V at10 m A cm^(-2)in OER. During long-term operation, the graphitic layers protect edge Fe-Nx sites from demetallation in ORR and synergize with Fe OOH species endowing Fe-N-G with enhanced OER activity.Density functional theory calculations reveal that the edge Fe-Nx site is superior to the in-plane Fe-Nx site in terms of OH* dissociation in ORR and OOH* formation in OER. The constructed ZAB based on Fe-N-G cathode shows a higher peak power density of 133 m W cm^(-2)and more stable cycling performance than Pt/C + RuO2counterparts. This work provides a novel strategy to obtain high-efficiency bifunctional oxygen electrocatalysts through space mediation. | Ruihui Gan Yali Wang Xiangwu Zhang Yan Song Jingli Shi Chang Ma | 2023 | Journal of Energy Chemistry2023，，8： | 0 |
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