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| 1 | Arabidopsis thaliana RECEPTOR DEAD KINASE1 Functions as a Positive Regulator in Plant Responses to ABA显示文摘Abscisic 酸(骆驼毛的织物) 是涉及重要压力相关、发展的植物过程的主要植物激素。限定膜的骆驼毛的织物信号 transduction 在早骆驼毛的织物发信号,而是连接核心发信号的分子的机制起一个重要作用到血浆膜的部件仍然保持不清楚。植物发展了很多像受体的 kinases (RLK ) 由察觉细胞外的刺激并且激活下游地发信号的回答调制多样的生物过程。在这研究,通常认为的充满白氨酸的 repeat-RLK 基因说出受体死了的 KINASE1 (AtRDK1 ) 在 Arabidopsis 被鉴别并且描绘 thaliana。RDK1 promoter-GUS 分析表明 RDK1 在 Arabidopsis 在各种各样的纸巾无所不在地被表示,并且它的表示被骆驼毛的织物主要导致。面对骆驼毛的织物, RDK1 缺乏的 rdk1-1 和 rdk1-2 线在子叶 greening 和根生长显示出重要抵抗,而 RDK1-overexpressing 线显示出提高的敏感。一致地,骆驼毛的织物应答的基因的表示是显著地在 rdk1 异种幼苗的 downregulated,它对有增加的水损失的干旱应力也过分敏感。有趣地, RDK1 被发现是不正常的 kinase 局部性到血浆膜并且没在幼苗开发的调停骆驼毛的织物的抑制期间要求它的 kinase 活动。因此, RDK1 与类型 2C 蛋白质磷酸酶 ABSCISIC 酸 INSENSITIVE1 (ABI1 ) 在血浆膜交往了;这个相互作用被骆驼毛的织物的外长的申请进一步提高,建议 ABI1 的调停 RDK1 的招募为骆驼毛的织物发信号是到血浆膜上重要的。一起拿,这些结果以一种骆驼毛的织物依赖者方式在对不能生活的压力条件的植物回答为 RDK1 揭示一个重要角色。 | Dhinesh Kumar Ritesh Kumar Dongwon Baek Tae-Kyung Hyun Woo Sik Chung Dae-Jin Yun Jae-Yean Kim | 2017 | Molecular Plant2017，10，2： | 10 |
| 2 | 用于通路(Gateway)克隆技术的植物表达载体研究进展显示文摘通路(Gateway)克隆技术是根据λ噬菌体基因组和大肠杆菌基因组之间的位点专一性重组分子机制开发的一套分子克隆新技术。利用该技术LR反应构建目的基因的表达载体时不需要经过酶切和连接等繁琐而又费时的过程,因此,可以节省很多时间。为了扩大Gateway技术在植物基因工程领域的应用,最近有很多研究机构和研究小组开发了能用于组成型或诱导型表达目的基因、基因沉默、启动子分析、蛋白质亚细胞定位、蛋白质/蛋白质相互作用、多个DNA片段的模块化组装和DNA组片段功能验证等研究用的植物表达载体。该文对这些技术的研究进展进行了综述。 | 肖素勤 孙振 轩秀霞 陈丽梅 | 2012 | 植物科学学报2012，30，5： | 6 |
| 3 | A Phytophthora capsid Effector Targets ACD11 Binding Partners that Regulate ROS-Mediated Defense Response in Arabidopsis显示文摘Reactive oxygen species (ROS) play a vital role in plant immune response, but the genes involved in the regulation of ROS are scantily reported. Phytophthora pathogens produce a large number of effectors to promote infection, but the modes of action adopted are largely unknown. Here, we report that RxLR207 could activate ROS-mediated cell death in Nicotiana benthamiana and was essential for virulence of P. capsici. We found that this effector targeted BPA1 (binding partner of ACD11) and four members of BPLs (BPAl-Like proteins)in Arabidopsis, and the bpa1 and bpl mutants had enhanced ROS accumulation and cell death under biotic or abiotic stresses. Furthermore, we showed that BPA1 and several BPLs functioned redundantly in plant immunity to P. capsici. We discovered that BPA1 and all six BPLs interacted with ACD11, and stabilization of ACD11 was impaired in the bpa 1, bpl2, bpl3, and bpl4 mutants. RxLR207 could promote the degradation of BPA1, BPL1, BPL2, and BPL4 to disrupt ACD11 stabilization in a 26S proteasome-dependent manner. Taken together, these fin dings indicate the important roles of Arabidopsis BPA1 and its homologs in ROS homeostasis and defense response, highlighting the usefulness of a pathogen effector-directed approach as a promising strategy for the discovery of novel plant immune regulators. | Qi Li Gan Ai Danyu Shen Fen Zou Ji Wang Tian Bai Yanyu Chen Shutian Li Meixiang Zhang Maofeng Jing Daolong Dou | 2019 | Molecular Plant2019，12，4： | 5 |
| 4 | Salt Stress Triggers Phosphorylation of the Arabidopsis Vacuolar K+ Channel TPK1 by Calcium-Dependent Protein Kinases (CDPKs)显示文摘 | Andreas Latz Norbert Mehlmer Simone Zapf Thomas D. Mueller Bernhard Wurzinger Barbara Pfister Edina Csaszar Rainer Hedrich Markus Teige Dirk Becker | 2013 | Molecular Plant2013，6，4： | 5 |
| 5 | A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species显示文摘Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains arduous.Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis thaliana.As an efficient high-throughput transient expression system is currently lacking in the model plant species A.thaliana,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in A.thaliana and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and N.benthamiana.Efficiency of this method was independently verified in three independent research facilities,pointing to the robustness of this technique.Furthermore,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis. | Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie | 2020 | Plant Communications2020，1，5： | 4 |
| 6 | The Fusarium oxysporum Avr2-Six5 Effector Pair Alters Plasmodesmatal Exclusion Selectivity to Facilitate Cell-to-Cell Movement of Avr2显示文摘病原体使用受动器蛋白质操作他们的主人。在西红柿的感染期间,真菌镰刀霉 oxysporum 分泌受动器 Avr2 和 Six5。而 Avr2 足够在 heterologous 系统触发 I-2-mediated 房间死亡,两个受动器在西红柿为 I-2-mediated 疾病抵抗被要求。Six5 怎么参予被触发的抵抗,是未知的。用双分子的荧光互补,我们发现了那 Avr2 和 Six5 的试金在 plasmodesmata 交往。单个房间的转变表明 2xRFP 标记蛋白质和 Avr2-GFP 仅仅面对 Six5 搬到附近的房间。因为 2xRFP 仅仅面对两个受动器是 translocated, Six5 独自不改变 plasmodesmatal transduction。在表示 SIX5 转基因的植物,病毒地表示的 Avr2-GFP 的分发,和 I-2-mediated 房间死亡的随后的发作,在野类型的西红柿不同于那。一起拿,我们的数据表演 ? 面对 Six5, Avr2 从房间搬到房间,它在易受影响的植物,但是在包含植物的 I-2 贡献毒力导致抵抗。 | Lingxue Cao Mila C. Blekemolen Nico Tintor Ben J.C. Cornelissen Frank L.W. Takken | 2018 | Molecular Plant2018，11，5： | 3 |
| 7 | 双分子荧光互补技术在神经系统变性疾病蛋白质寡聚化研究中的应用显示文摘神经系统变性疾病(ND)的病理特征是蛋白质聚集在细胞内或细胞外形成异常的堆积体而导致神经毒性。双分子荧光互补技术(Bi FC)是研究ND相关蛋白质寡聚化分子机制的重要工具。与其他技术不同的是,Bi FC不但可以在生理环境中对蛋白质-蛋白质之间的相互作用进行可视化检测,而且能够提供详细的亚细胞定位信息。本文通过综述Bi FC技术的原理、优缺点及其在ND研究领域中的应用进展,探讨该技术对揭示寡聚体和包涵体形成原因方面的潜力,为神经系统疾病开发新的治疗策略提供重要参考。 | 范红波 陈军芳 许杰 肖严 黄欣媛 | 2015 | 现代生物医学进展2015，15，2： | 1 |
| 8 | 拟南芥ROP2与GGB蛋白互作的分析显示文摘为了阐明拟南芥ROP蛋白家族成员ROP2与蛋白异戊二烯化中I型香叶酰基的β亚基GGB的互作关系,本研究先后采用酵母双杂交和三杂交技术分析了两者之间的相互作用。结果表明,ROP2与GGB之间在酵母细胞中并没有发生相互作用。我们又采用双分子荧光互补(BiFC)技术在植物体内再次验证两者之间的相互作用。重新构建了一套带多克隆位点的常规BiFC重组表达载体,基于这套BiFC载体,进行了验证分析。结果表明,实验组在本氏烟草的表皮细胞中发现了黄色荧光信号,并且信号定位在细胞膜上,而阴性对照组未发现荧光信号,说明GGB蛋白与ROP2蛋白在植物体内存在相互作用,并且这种相互作用发生在细胞膜上。本研究结果为进一步揭示ROP2的生化调控机制奠定了前期基础。 | 杨迷 胡燕 李继洋 李月 刘超 代培红 刘晓东 | 2020 | 分子植物育种2020，18，7： | 1 |
| 9 | The novel peptide Nb PPI1 identified from Nicotiana benthamiana triggers immune responses and enhances resistance against Phytophthora pathogens显示文摘In plants, recognition of small secreted peptides, such as damage/danger-associated molecular patterns(DAMPs), regulates diverse processes,includingstressandimmune responses. Here, we identified an SGPS(Ser-Gly-Pro-Ser) motif-containing peptide, Nicotiana tabacum NtPROPPI, and its two homologs in Nicotiana benthamiana, NbPROPPI1 and NbPROPPI2. Phytophthora parasitica infection and salicylic acid(SA) treatment induced NbPROPPI1/2 expression. Moreover, SignalP predicted that the 89-amino acid NtPROPPI includes a 24-amino acid N-terminal signal peptide and NbPROPPI1/2-GFP fusion proteins were mainly localized to the periplasm. Transient expression of NbPROPPI1/2 inhibited P.parasiticacolonization,andNbPROPPI1/2 knockdown rendered plants more susceptible to P. parasitica. An eight-amino-acid segment in the NbPROPPI1 C-terminus was essential for its immune function and a synthetic 20-residue peptide, NbPPI1, derived from the C-terminus of NbPROPPI1 provoked significant immune responses in N. benthamiana. These responses led to enhanced accumulation of reactive oxygen species, activation of mitogen-activated protein kinases, and up-regulation of the defense genes Flg22-induced receptor-like kinase(FRK) and WRKY DNA-binding protein 33(WRKY33). The NbPPI1-induced defense responses require Brassinosteroid insensitive1-associated receptor kinase 1(BAK1). These results suggest that NbPPI1 functions as a DAMP in N. benthamiana;this novel DAMP provides a potentially useful target for improving plant resistance to Pytophthora pathogens. | Qujiang Wen Manli Sun Xianglan Kong Yang Yang Qiang Zhang Guiyan Huang Wenqin Lu Wanyue Li Yuling Meng Weixing Shan | 2021 | Journal of Integrative Plant Biology2021，63，5： | 1 |
| 10 | Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta显示文摘An ever-increasing number of intracellular multi-protein networks have been identified in plant cells.Split-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex localization.Because of their simple protocols,they have become some of the most frequently used methods.However,standard fluorescent proteins present several drawbacks for sophisticated microscopy.With the HaloTag system,these drawbacks can be overcome,as this reporter forms covalent irreversible bonds with synthetic photostable fluorescent ligands.Dyes can be used in adjustable concentrations and are suitable for advanced microscopy methods.Therefore,we have established the Split-HaloTag imaging assay in plants,which is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent ligand.Its suitability and robustness were demonstrated using a well-characterized interaction as an example of protein–protein interaction at cellular structures:the anchoring of the molybdenumcofactor biosynthesis complex to filamentous actin.In addition,a specific interactionwas visualized in a more distinctivemannerwith subdiffractional polarizationmicroscopy,Airyscan,and structured illumination microscopy to provide examples of sophisticated imaging.Split-GFPand Split-HaloTag can complement one another,as Split-HaloTag represents an alternative option and an addition to the large toolbox of in vivo methods.Therefore,this promising new Split-HaloTag imaging assay provides a unique and sensitive approach formore detailed characterization of protein–protein interactions using specific microscopy techniques,such as 3D imaging,single-molecule tracking,and super-resolution microscopy. | Rieke Minner-Meinen Jan-Niklas Weber Andreas Albrecht Rainer Matis Maria Behnecke Cindy Tietge Stefan Frank Jutta Schulze Henrik Buschmann Peter Jomo Walla Ralf-R.Mendel Robert Hänsch David Kaufholdt | 2021 | Plant Communications2021，2，5： | 0 |
| 11 | Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion显示文摘根头发是由尖端生长,与花粉试管分享的一个过程,轴突,和真菌的 hyphae 发展的单个房间。然而,结构的植物细胞墙强加限制完成尖端生长。除了多糖,植物房间墙由 hydroxyproline 富有的 glycoproteins (HRGP ) 组成,它包括几组 O-glycoproteins,包括 extensins (EXT ) 。脯氨酸 hydroxylation, HRGP 的早 translational 以后修正(PTM ) 由 prolyl 4-hydroxylases (P4Hs ) 催化,定义他们的随后的 O-glycosylation 地点。在这个工作,我们的基因分析证明那 P4H5,并且到更小的程度 P4H2 和 P4H13,为根头发尖端生长是枢轴的。第二,我们证明 P4H5 把 invitro 比较喜欢为 EXT 底层而非为另外的 HRGP 的特性。第三由交换途径的 P4H 倡导者和蛋白质, weshow 那 P4H2 和 P4H13 有可互换的功能,但是不能代替 P4H5。这三 P4Hs 被显示被指向到能分泌的小径,在 P4H5 与 P4H2 和 P4H13 形成 dimers 的地方。最后,我们在房间墙建筑学上探索缺乏的脯氨酸 hydroxylation 的影响。一起拿,我们的结果支持在哪个在 EXT 上改正 peptidyl 脯氨酸 hydroxylation 的一个模型,并且可能在另外的 HRGP,在 Arabidopsis thaliana 为自己组装的合适的房间墙和因此根头发延伸被要求。 | Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen Petersen Norberto D. lusem Jose M. Estevez | 2015 | Molecular Plant2015，8，5： | 0 |
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