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您的检索式：作者名="Henrik Buschmann"
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| 1 | Arabidopsis Mutants and the Network of M icrotu bu le-Associated Functions显示文摘在早优核质,微导管系统从事有丝分裂,细胞内部的运输,和基于鞭子的活动性。在植物系,多细胞的身体的进化包含了一些核心函数,其它的损失,和与多细胞的植物习惯联系的新微导管函数的详尽阐述的保存。这多样化被保存的两个(animal/fungi-like ) 和小说的存在反映(似植物) 把微导管相关的功能编码为 Arabidopsis 染色体的序列。微导管异种的收集在最近的年很快成长了。这些异种介绍大量显型,与微导管系统的功能的多样化的假设一致。在这评论,我们集中于变异的分析并且特别地,为指出基因功能的小径作为一个珍贵工具讨论两倍变异的分析。未来挑战将是在植物定义微导管功能的基因、物理的相互作用的完全的网络。除了在 MAPome 的功能的分析考察最近的进步,我们在场在微导管损害的 Arabidopsis 异种的一个联机数据库工作。 | Henrik Buschmann Clive W. Lloyd | 2008 | Molecular Plant2008，1，6： | 6 |
| 2 | Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta显示文摘An ever-increasing number of intracellular multi-protein networks have been identified in plant cells.Split-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex localization.Because of their simple protocols,they have become some of the most frequently used methods.However,standard fluorescent proteins present several drawbacks for sophisticated microscopy.With the HaloTag system,these drawbacks can be overcome,as this reporter forms covalent irreversible bonds with synthetic photostable fluorescent ligands.Dyes can be used in adjustable concentrations and are suitable for advanced microscopy methods.Therefore,we have established the Split-HaloTag imaging assay in plants,which is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent ligand.Its suitability and robustness were demonstrated using a well-characterized interaction as an example of protein–protein interaction at cellular structures:the anchoring of the molybdenumcofactor biosynthesis complex to filamentous actin.In addition,a specific interactionwas visualized in a more distinctivemannerwith subdiffractional polarizationmicroscopy,Airyscan,and structured illumination microscopy to provide examples of sophisticated imaging.Split-GFPand Split-HaloTag can complement one another,as Split-HaloTag represents an alternative option and an addition to the large toolbox of in vivo methods.Therefore,this promising new Split-HaloTag imaging assay provides a unique and sensitive approach formore detailed characterization of protein–protein interactions using specific microscopy techniques,such as 3D imaging,single-molecule tracking,and super-resolution microscopy. | Rieke Minner-Meinen Jan-Niklas Weber Andreas Albrecht Rainer Matis Maria Behnecke Cindy Tietge Stefan Frank Jutta Schulze Henrik Buschmann Peter Jomo Walla Ralf-R.Mendel Robert Hänsch David Kaufholdt | 2021 | Plant Communications2021，2，5： | 0 |
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