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| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | CD4+ CD25highCD127low regulatory T cells in peripheral blood are not an independent factor for chronic graft-versus-host disease after allogeneic stem cell transplantation显示文摘 | Perz J B Gürel S Schonland S O | | 0，，： | 1 |
| 3 | Direct evaluation of the sp 3 content in diamond-like-carbon films by XPS显示文摘 | P Mérel M Tabbal M Chaker S Moisa J Margot | 1998 | Applied Surface Science1998，，1： | 1 |
| 4 | Pitfalls of high-pass filtering for detecting epileptic oscillations:a technical note on'false'ripples显示文摘 | BénarCG ChauvièreL BartolomeiF | 2010 | Clin Neurophysiol2010，121，3： | 1 |
| 5 | Analysis of dielectric photonic-crystal problems with MLFMA and schur-complement preconditioners显示文摘 | Ergül O Malas T Gürel L | 2011 | Lightwave Technology2011，29，6： | 1 |
| 6 | Regulated expression of Arabidopsis Shaker K+ channel genes involved in K+ uptake and distribution in the plant显示文摘 | Guillaume Pilot Frédéric Gaymard Karine Mouline Isabelle Chérel Hervé Sentenac | 2003 | Plant Molecular Biology2003，，5： | 1 |
| 7 | On Name-based Inter-domain Routing显示文摘 | Rajahalme J S?rel? M Visala K | 2011 | Computer Networks2011，55，4： | 1 |
| 8 | Optimization o{ oligonucleotide-hased DNA microarrays显示文摘 | REL GIO A SCHWAGER C RICHTER A | 2002 | Nucleic Acids Research2002，30，11： | 1 |
| 9 | Prevalence of myocardial bridging and correlation with coronary atherosclerosis studied with 64-slice CT coronary angiography显示文摘 | Gruttal IL Runzal G Rel G | 2009 | Radiolmed2009，114，： | 1 |
| 10 | Mean platelet volume and D-Dimer in patients with suspected deep venous thrombosis显示文摘 | Canan A Halicio lu SS Gürel S | | 0，，02： | 1 |
| 11 | Different brain mechanisms mediate two strategies in arithmetic:evidence from event-related brain potentials显示文摘 | Yagoubi REl Lemaire P Besson M | 2003 | Neuropsychologia2003，41，： | 1 |
| 12 | CA 125 levels and left ventricular function in patients with end-stage renal disease on maintenance hemodialysis显示文摘 | Yilmaz H Gürel OM Celik HT | 2014 | Ren Fail2014，36，2： | 1 |
| 13 | Optimization of gene transfer into barley (Hordeum vulgare L.) mature embryos by tissue electroporation显示文摘 | F. Gürel N. G?zükirmizi | 2000 | Plant Cell Reports2000，，8： | 1 |
| 14 | Comparison of red cell distribution width and a red cell discriminant function incorporating volume dispersion for distinguishing iron deficiency form beta thalassemia trait in patients with microcytosis 显示文摘 | Lima CS Rels AR Grotto HZ | 1996 | Revista Paulista de Medicina1996，114，5： | 1 |
| 15 | Multivariate Statistical Techniques for the Assessment of Surface Water Quality at the Mid-Black Sea Coast of Turkey显示文摘 | Feryal Akbal Levent Gürel Tolga Bahadr | 2011 | Water Air Soil Pollut2011，216，： | 1 |
| 16 | Mean platelet volume and D-dimer in patients with suspected deep venous thrombosis显示文摘 | Arzu Canan S?dd?ka Sezgin Hal?c?o?lu Safiye Gürel | 2012 | Journal of Thrombosis and Thrombolysis2012，，2： | 1 |
| 17 | Fractional - order Wien - bridge oscillator显示文摘 | W Ahmad REl Khazali A El - wakil | 2001 | Electr Lett2001，37，： | 1 |
| 18 | Predominant VNTR family of strains of Mycobacterium tuberculosis isolated from South Asian patients 显示文摘 | Gascoyne- Binzi DM Barlow REL Essex A | 2002 | Int J Tuberc Lung Dis2002，6，： | 1 |
| 19 | Flow injection analysis of sulphide based on its photoelectrocatalytic oxidation at poly-methylene blue modified glassy carbon electrode显示文摘 | Yusuf Dilgin Seda Canarslan Onder Ayyildiz Bensu Ertek Gürel Ni?li | 2012 | Electrochimica Acta2012，，： | 1 |
| 20 | Exact calibration of programming models of agricultural supply against exogenous supply elasticities显示文摘 | Pierre Mé rel Santiago Bucaram | 2010 | European Review of Agricultural Economics2010，，3： | 1 |
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