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| 1 | An Uncanonical CCCH-Tandem Zinc-Finger Protein Represses Secondary Wall Synthesis and Controls Mechanical Strength in Rice显示文摘第二等的墙,代表生物资源的体积,在植物生长和改编上有大影响到环境。第二等的墙合成被一个复杂发信号 transduction 网络交换并且调整。然而,有这些规章的小径的有限理解。这里,我们报导(IIP4 )交往 ILA1 蛋白质 4 能镇压第二等的墙合成。IIP4 是象 Raf 一样 MAPKKK 的 phosphorylation 底层,但是它的功能是未知的。由产生 iip4 异种和相关转基因的植物,我们发现在 IIP4 的损害提高第二等的墙形成。基因表示和 transactivation 活动试金表明 IIP4 否定地调整 MYB61 和 CESA 的表示但是不绑 ? 他们的倡导者。IIP4 与 NAC29/NAC31 交往,第二等的墙合成的在上游的管理者,并且在植物压制下游地规章的小径。显示出的 Mutagenesis 分析那 phosphomimic IIP4 从原子核的蛋白质 translocate 到细胞质。而且,我们表明 IIP evolutionarily 被保存并且分享 unreported CCCH 主题,叫作 uncanonical CCCH 双人脚踏车锌手指蛋白质。一起,我们的学习提供机械学的卓见进第二等的墙合成的控制并且为在庄稼改进相关农学的特点介绍一个机会。 | Dongmei Zhang Zuopeng Xu Shaoxue Cao Kunling Chen Shance Li Xiangling Liu Caixia Gao Baocai Zhang Yihua Zhou | 2018 | Molecular Plant2018，11，1： | 14 |
| 2 | The RLCK-VND6 module coordinates secondary cell wall formation and adaptive growth in rice显示文摘The orderly deposition of secondary cell wall(SCW)in plants is implicated in various biological programs and is precisely controlled.Although many positive and negative regulators of SCW have been documented,the molecular mechanisms underlying SCW formation coordinated with distinct cellular physiological processes during plant adaptive growth remain largely unclear.Here,we report the identification of Cellulose Synthase co-expressed Kinase1(CSK1),which encodes a receptor-like cytoplasmic kinase,as a negative regulator of SCW formation and its signaling cascade in rice.Transcriptome deep sequencing of developing internodes and genome-wide co-expression assays revealed that CSK1 is co-expressed with cellulose synthase genes and is responsive to various stress stimuli.The increased SCW thickness and vigorous vessel transport in csk1 indicate that CSK1 functions as a negative regulator of SCW biosynthesis.Through observation of green fluorescent protein-tagged CSK1 in rice protoplasts and stable transgenic plants,we found that CSK1 is localized in the nucleus and cytoplasm adjacent to the plasma membrane.Biochemical and molecular assays demonstrated that CSK1 phosphorylates VASCULAR-RELATED NAC-DOMAIN 6(VND6),a master SCW-associated transcription factor,in the nucleus,which reduces the transcription of a suite of SCW-related genes,thereby attenuating SCW accumulation.Consistently,genetic analyses show that CSK1 functions upstream of VND6 in regulating SCW formation.Interestingly,our physiological analyses revealed that CSK1 and VND6 are involved in abscisic acid-mediated regulation of cell growth and SCW deposition.Taken together,these results indicate that the CSK1-VND6 module is an important component of the SCW biosynthesis machinery,which coordinates SCW accumulation and adaptive growth in rice.Our study not only identifies a new regulator of SCW biosynthesis but also reveals a fine-tuned mechanism for precise control of SCW deposition,offering tools for rationally tailoring agronomic traits. | Shaoxue Cao Yan Wang Yihong Gao Rui Xu Jianing Ma Zuopeng Xu Keke Shang-Guan Baocai Zhang Yihua Zhou | 2023 | Molecular Plant2023，16，6： | 0 |
| 3 | Effect of CuO addition on the sintering temperature and microwave dielectric properties of CaSiO_3–Al_2O_3 ceramics显示文摘CuO-doped CaSiO3–1 wt% Al2O3 ceramics were synthesized via a traditional solid-state reaction method, and their sintering behavior,microstructure and microwave dielectric properties were investigated. The results showed that appropriate CuO addition could accelerate the sintering process and assist the densification of CaSiO3–1 wt% Al2O3 ceramics, which could effectively lower the densification temperature from1250 1C to 1050 1C. However, the addition of CuO undermined the microwave dielectric properties. The optimal amount of CuO addition was found to be 0.8 wt%, and the derived CaSiO3–Al2O3ceramic sintered at 1100 1C presented good microwave dielectric properties of εr?7.27,Q f?16,850 GHz and τf? 39.53 ppm/1C, which is much better than those of pure CaSiO3 ceramic sintered at 1340oC(Q f?13,109 GHz).The chemical compatibility of the above ceramic with 30 Pd/70 Ag during the cofiring process has also been investigated, and the result showed that there was no chemical reaction between palladium–silver alloys and ceramics.& 2014 Chinese Materials Research Society. Production and hosting by Elsevier B.V. All rights reserved. | Denghao Li Huanping Wang Zuopeng He Zhen Xiao Ruoshan Lei Shiqing Xu | 2014 | Progress in Natural Science:Materials International2014，24，3： | 0 |
| 4 | Identification and fine mapping of a fertility restorer gene for wild abortive cytoplasmic male sterility in the elite indica rice non-restorer line 9311显示文摘The wild abortive(WA)-type cytoplasmic male sterility(CMS)derived from the wild rice species Oryza rufipogon Griff.is used widely in three-line indica hybrids.The identification and mapping of restorer of fertility(Rf)genes aided in the development of WA-type hybrids.Here we report that testcross F1 plants from the WA-type CMS line and 9311 exhibited stainable pollen grains with no seed set,indicating that 9311 carries minor-effect Rfs for WA-type CMS.We developed an advanced backcross population consisting of plants harboring small regions of donor chromosomal segments from 9311 in the WATianfeng A genetic background with moderate seed setting rates.Genetic analysis showed that the pollen fertility levels of the backcross individuals are governed by a single gene from 9311 that we named Rf19(t).By use of the RICE 40 K gene chip,three introduced segments were identified in the fertile lines,and a candidate region spanning 4.37–8.29 Mb on chromosome 1 was identified for Rf19(t).Finally,Rf19(t)was fine-mapped to a region of 90 kb between the DNA marker loci STS1-163 and STS1-183,in which eight ORFs were predicted.Also,using relative expression analyses,comparative sequence analyses and functional domain analyses,we identified LOC_Os01g10530 as the most likely candidate gene for Rf19(t).Furthermore,Rf19(t)was found to function in fertility restoration,most probably by regulating the degradation of m RNA transcribed from the mitochondrial gene WA352.These results increase our knowledge of fertility restoration in WA-type CMS lines and will facilitate the development of high-quality pairs of WAtype CMS and maintainer lines. | Zuopeng Xu Yuanyue Du Xixu Li Ruixuan Wang Zhi'ai Chen Xiangqiang Zhao Qiaoquan Liu Shuzhu Tang Honggen Zhang | 2023 | The Crop Journal2023，11，3： | 0 |
| 5 | Suppression of HIV-1 Integration by Targeting HIV-1 Integrase for Degradation with A Chimeric Ubiquitin Ligase显示文摘Human immunodeficiency virus(HIV) attacks human immune system and causes life-threatening acquired immune deficiency syndrome(AIDS). Treatment with combination antiretroviral therapy(cART) could inhibit virus growth and slow progression of the disease, however, at the same time posing various adverse effects. Host ubiquitin-proteasome pathway(UPP) plays important roles in host immunity against pathogens including viruses by inducing degradation of viral proteins. Previously a series of methods for retargeting substrates for ubiquitin-proteasome degradation have been successfully established. In this study, we attempted to design and construct artificial chimeric ubiquitin ligases(E3 s) based on known human E3 s in order to manually target HIV-1 integrase for ubiquitin proteasome pathway-mediated degradation.Herein, a series of prototypical chimeric E3 s have been designed and constructed, and original substrate-binding domains of these E3 s were replaced with host protein domains which interacted with viral proteins. After functional assessment screening, 146 LI was identified as a functional chimeric E3 for HIV-1 NL4-3 integrase. 146 LI was then further optimized to generate 146 LIS(146 LI short) which has been shown to induce Lys48-specific polyubiquitination and reduce protein level of HIV-1 NL4-3 integrase more effectively in cells. Lymphocyte cells with 146 LIS knock-in generated by CRISPR/Cas-mediated homology-directed repair(HDR) showed remarkably decreased integration of HIV-1 NL4-3 viral DNAs and reduced viral replication without obvious cell cytotoxicity. Our study successfully obtained an artificial chimeric E3 which can induce Lys48-specific polyubiquitination and proteasome-mediated degradation of HIV-1 NL4-3 integrase, thus effectively inhibiting viral DNA integration and viral replication upon virus infection. | Zuopeng Zhang Sen Yuan Shuting Xu Deyin Guo Lang Chen Wei Hou Min Wang | 2021 | Virologica Sinica2021，36，3： | 0 |
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