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| 1 | Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development显示文摘在繁殖开发期间,米饭植物开发决定最后的谷物收益的唯一的花器官。OsMADS1,象 SEPALLATA 一样疯盒子的基因之一,被解开了玩关键角色 inrice 花的机关身份说明和花的分裂组织确定性。然而,在调整花开发与另外的花的 homeotic 基因位于 OsMADS1 的相互作用下面的分子的机制 remainslargely 逃犯。在这个工作,我们在他们的蛋白质之中与物理相互作用一起与 B- , C- ,和 D 班基因学习了 OsMADS1 的基因相互作用。我们证明在 OsMADS1 和 OsMADS3 之间的物理、基因的相互作用为花的分裂组织活动维护和机关身份说明是必要的;当 OsMADS1 身体上并且遗传上在调整花的分裂组织确定性和 suppressingspikelet 分裂组织回复与 OsMADS58 交往时。我们提供了重要基因证据在花开发期间支持二米饭 C 班基因(OsMADS3 和 OsMADS58 ) 的 neofunctionalization。基因表示介绍和量的 RT-PCR 分析进一步表明 OsMADS1 影响涉及发信号的花的身份和荷尔蒙的许多基因的表达式,并且染色质 immunoprecipitation (芯片)-PCR 试金进一步证明 OsMADS17 是 OsMADS1 的直接目标基因。一起拿,这些结果表明 OsMADS1 在指定米饭有多样化的规章的功能花的机关和分裂组织身份,可能通过它和不同花的 homeotic 管理者的基因、物理的相互作用。 | Yun Hu Wanqi Liang Changsong Yin Xuelian Yang Baozhe Ping Anxue Li Ru Jia Mingjiao Chen Zhijing Luo Qiang Cai Xiangxiang Zhao Dabing Zhang Zheng Yuan | 2015 | Molecular Plant2015，8，9： | 10 |
| 2 | Effects of stick-slip on stress intensity factors for subsurface short cracks in rolling contact显示文摘This paper theoretically investigates the effects of stick-slip in rolling contact zone on stress intensity factors(SIFs)for subsurface short cracks.New mathematical models for SIFs including stick-slip ratio are deduced in two cases.One is a subsurface short crack parallel to surface,and the numerical analysis shows that the value of KⅡincreases with the increase of stick-slip ratio;the other is a subsurface short crack perpendicular to the surface,and the numerical analysis indicates that the positive value of KⅠdecreases with the increase of stick-slip ratio.As △KⅠand △KⅡare necessary to evaluate the fatigue crack propagation rate or fatigue lifetime,the influences of stick-slip ratio on them are then discussed.It is found that the maximum variations of △KⅠand △KⅡare both around 3.0%due to stick-slip ratio variation. | LIU WenTao ZHANG Yun FENG ZhiJing ZHAO JingShan | 2013 | Science China(Technological Sciences)2013，56，10： | 6 |
| 3 | Investigation of CRISPR/Cas9-induced SD1 rice mutants highlights the importance of molecular characterization in plant molecular breeding显示文摘Although Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/CRISPR-associated 9(Cas9)system has been widely used for basic research in model plants,its application for applied breeding in crops has faced strong regulatory obstacles,due mainly to a poor understanding of the authentic output of this system,particularly in higher generations.In this study,different from any previous studies,we investigated in detail the molecular characteristics and production performance of CRISPR/Cas9-generated SD1(semi-dwarf 1)mutants from T2 to T4 generations,of which the selection of T1 and T2 was done only by visual phenotyping for semidwarf plants.Our data revealed not only on-and off-target mutations with small or lager indels but also exogenous elements in T2 plants.All indel mutants passed stably to T3 or T4 without additional modifications independent on the presence of Cas9,while some lines displayed unexpected hereditary patterns of Cas9 or some exogenous elements.In addition,effects of various SD1 alleles on rice height and yield differed depending on genetic backgrounds.Taken together,our data showed that the CRISPR/Cas9 system is effective in producing homozygous mutants for functional analysis,but it may be not as precise as expected in rice,and that early and accurate molecular characterization and screening must be carried out for generations before transitioning of the CRISPR/Cas9 system from laboratory to field. | Sukumar Biswas Jiaqi Tian Rong Li Xiaofei Chen Zhijing Luo Mingjiao Chen Xiangxiang Zhao Dabing Zhang Staffan Persson Zheng Yuan Jianxin Shi | 2020 | Journal of Genetics and Genomics2020，47，5： | 4 |
| 4 | Micro-blogs Entity Recognition Based on DSTCRF显示文摘We proposed a unified model for Chinese named entity recognition in micro-blogs. The models provide a simple statistical framework to incorporate a wide variety of linguistic knowledge and statistical models in a unified way. In our approach, KNN classifier is used to get suitable training data. An optimal algorithm to generate the hierarchically structured DSTCRF is executed to select the structure attributes of the named entity in micro-blogs knowledge. The experimental results showed that the accuracy rate was significantly improved. | WANG Jing LIU Zhijing ZHAO Hui | 2014 | Chinese Journal of Electronics2014，23，1： | 3 |
| 5 | OsMADS32 interacts with PI-like proteins and regulates rice flower development显示文摘OsMADS32 is a monocot specific MIKC^c type MADS-box gene that plays an important role in regulating rice floral meristem and organs identity, a crucial process for reproductive success and rice yield. However, its underlying mechanism of action remains to be clari fied. Here, we characterized a hypomorphic mutant allele of OsMADS32/CFO1, cfo1-3 and identi fied its function in controlling rice flower development by bioinformatics and protein-protein interaction analysis. The cfo1-3 mutant produces defective flowers,including loss of lodicule identity, formation of ectopic lodicule or hull-like organs and decreased stamen number,mimicking phenotypes related to the mutation of B class genes. Molecular characterization indicated that mis-splicing of OsMADS32 transcripts in the cfo1-3 mutant resulted in an extra eight amino acids in the K-domain of OsMADS32 protein.By yeast two hybrid and bimolecular fluorescence complementation assays, we revealed that the insertion of eight amino acids or deletion of the internal region in the K1 subdomain of Os MADS32 affects the interaction between OsMADS32 with PISTILLATA(PI)-like proteins OsMADS2 and OsMADS4. This work provides new insight into the mechanism by which Os MADS32 regulates rice lodicule and stamen identity, by interaction with two PI-like proteins via its K domain. | Huanhuan Wang Liang Zhang Qiang Cai Yun Hu Zhenming Jin Xiangxiang Zhao Wei Fan Qianming Huang Zhijing Luo Mingjiao Chen Dabing Zhang Zheng Yuan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 2 |
| 6 | Evaluation of short--term psychological functions in opiate addicts af- ter ablating the nucleus accumbens via stereotactic sur gery显示文摘 | Fei He Hao Guan Zhijing Zhao | 2008 | Stereotact Funct Neurosurg2008，86，5： | 1 |
| 7 | The mechanism theory and application of deployable structures based on SLE显示文摘 | ZHAO Jingshan CHU Fulei FENG Zhijing | 2009 | Mechanism and Machine Theory2009，44，2： | 1 |
| 8 | Analysis of the singularity of spatial parallel manipulator with terminal constraints显示文摘 | ZHAO Jingshan FENG Zhijing ZHOU Kai | 2005 | Mechanism and Machine Theory2005，40，： | 1 |
| 9 | Cognitive radio spectrum allocation using evolution algorithms显示文摘 | Zhao Zhijing Peng Zheng Zheng Shilian | 2009 | IEEE Trans on Wireless Communications2009，8，9： | 1 |
| 10 | The Mechanism Theory and Application of Deployable Structures Based on SLE显示文摘 | Zhao Jingshan Chu Fulei Feng Zhijing | 2009 | Mechanism and Machine Theory2009，44，2： | 1 |
| 11 | Recognizing human activities using non-linear SVM decision tree显示文摘 | Zhao Haiyong Liu Zhijing Zhang Hao | 2011 | Intelligent Computing and Information Sicence2011，134，7： | 1 |
| 12 | Human actionrecognition using the image contour 显示文摘 | ZHAO Haiyong LIU Zhijing ZHANG Hao | 2010 | Journal of Optoelectr- orics-Laser2010，，10： | 1 |
| 13 | Establishment and Application of Heminested RT-PCR Assay for Detection of Mosquito-Borne Flavivirus—Guizhou Province,China,2018显示文摘Introduction:Mosquito-borne flavivirus can lead to serious infectious diseases worldwide and cause high mortality and disability.In order to strengthen epidemiological investigation of flavivirus and meet the needs of early warning of diseases,a simple,rapid,and sensitive detection method needs to be established to prevent and control mosquito-borne diseases.Methods:Using the NS5 gene of flavivirus in GenBank,3 universal primers targeting the conserved regions were designed.The complementary DNAs(cDNAs)of Japanese encephalitis virus(JEV),dengue virus(DENV),West Nile virus(WNV),and yellow fever virus(YFV)were used as the template to optimize the reaction conditions.A heminested reversetranscriptase polymerase chain reaction(hnRT-PCR)was established to verify the sensitivity and specificity of this method and to detect field-caught mosquitoes.Results:Our results showed that this method exhibited better specificity,higher sensitivity,and the ability to detect multiple viruses simultaneously.The lowest detection limit of JEV,DENV-2,YFV,and WNV was 3×10^(4),3×10^(6),3×10^(5),and 3×10^(4)copies/μL,respectively.Mosquito-borne flavivirus was successfully detected in the field-caught mosquito samples using the method established in this study.Discussion:The hnRT-PCR method established in this study can be employed for the rapid detection of flavivirus and provide technical support for early and rapid diagnosis of mosquito-borne flavivirus. | Zhijing Xue Ning Zhao Jun Wang Xiuping Song Fengxia Meng Wenqin Liang Jingzhu Zhou Dan Wang Zhong Zhang Qiyong Liu | 2021 | China CDC weekly2021，3，1： | 1 |
| 14 | Exploring User Influence for Topical Group Recommendation显示文摘With the development of social networks,many recommendation systems recommend items to a group of users, known as group recommendation. However, it will not be an appropriate recommendation without user topical influence analysis. We proposed a new group recommendation based on user topical influence analysis.We firstly construct several topical sub-groups depending on topics. Then we analyze user topical influence in subgroup, including user influence on specific topic and the topical sub-group. Besides, four user-factors are introduced to calculate the user social influence on topical sub-groups more accurately. Based on user topical influence analysis,we present our topical group recommendation algorithm,which calculates the predicted rating value for sub-group by aggregating weighted ratings of all users in the subgroup. The experimental results show convincingly that our proposed method can improve the group recommendation quality. | WANG Jing ZHAO Hui LIU Zhijing | 2017 | Chinese Journal of Electronics2017，26，1： | 1 |
| 15 | The mechanismtheory and application of deployable structures based onSLE显示文摘 | Zhao Jingshan Chu Fulei Feng Zhijing | 2009 | Mechanism and Machine Theory2009，44，32： | 1 |
| 16 | Effects of operating conditions on the performance degradation and anode microstructure evolution of anode-supported solid oxide fuel cells显示文摘Performance degradation shortens the life of solid oxide fuel cells in practical applications.Revealing the degradation mechanism is crucial for the continuous improvement of cell durability.In this work,the effects of cell operating conditions on the terminal voltage and anode microstructure of a Ni-yttria-stabilized zirconia anode-supported single cell were investigated.The microstructure of the anode active area near the electrolyte was characterized by laser optical microscopy and focused ion beam-scanning electron microscopy.Ni depletion at the anode/electrolyte interface region was observed after 100 h discharge tests.In addition,the long-term stability of the single cell was evaluated at 700℃for 3000 h.After an initial decline,the anode-supported single cell exhibits good durability with a voltage decay rate of 0.72%/kh and an electrode polarization resistance decay rate of 0.17%/kh.The main performance loss of the cell originates from the initial degradation. | Xin Yang Zhihong Du Qian Zhang Zewei Lyu Shixue Liu Zhijing Liu Minfang Han Hailei Zhao | 2023 | International Journal of Minerals,Metallurgy and Materials2023，30，6： | 1 |
| 17 | NSVM Decision Tree for Multi-class Classification显示文摘 | YAO Yong ZHAO Hui LIU Zhijing | 2008 | Chinese Journal of Electronics2008，17，4： | 1 |
| 18 | Bioremediation Process and Bioremoval Mechanism of Heavy Metal Ions in Acidic Mine Drainage显示文摘 | ZHAO Ying FU Zhijing CHEN Xiaofei ZHANG Guoyan | 2018 | Chemical Research in Chinese Universities2018，34，1： | 1 |
| 19 | Improving sample preparation to investigate lignin intensity of xylem at the cellular level by confocal Raman microspectroscopy of Populus tomentosa显示文摘Confocal Raman microspectroscopy(CRM)is an important tool for analyzing the compositional distribution of cell walls in situ.In this study,we improved the sample preparation method using paraffin-embedded sections combined with hexane dewaxing to obtain high resolution Raman images.We determined that the cell wall components of fiber cells were different from those of ray cells and vessel cells in the xylem of Populus tomentosa.Acetyl bromide and CRM methods produced similar trends when the difference in lignin intensity in the xylem region was compared between transgenic PtrLac4 and wild-type P.tomentosa.However,CRM proved more useful to analyze the lignin distribution in each cell type and distinguished the detailed difference in lignin intensity at the cellular level.Thus,CRM proved to be a useful in situ method to rapidly analyze the spatial variation of lignin content in the xylem of woody plants. | Bing Wang Mei Luo Yadi Liu Xiaorui Guo Xiatong Liu Chong Zhang Zhijing Zhao Di Liu Hui Li Hai Lu | 2021 | Journal of Forestry Research2021，32，5： | 0 |
| 20 | Impact of preoperative therapy on surgical outcomes of laparoscopic total gastrectomy for gastric/gastroesophageal junction cancer显示文摘Objective: As laparoscopic surgery is widely applied for primarily treated gastric cancer(GC)/gastroesophageal junction cancer(GEJC) and gains many advantages, the feasibility of laparoscopic total gastrectomy(LTG) for GC/GEJC patients who have received preoperative therapy(PT) has come to the fore. This study aims to analyze the safety and feasibility of LTG after PT for GC/GEJC patients.Methods: We retrospectively analyzed the data of 511 patients with GC/GEJC undergoing LTG, of which 405received LTG(LTG group) and 106 received PT+LTG(PT-LTG group) at Nanfang Hospital between June 2018and September 2022. The surgical outcomes were compared between the two groups.Results: The surgical duration was significantly longer in the PT-LTG group(P<0.001), while the incidence of intraoperative complications(P=1.000), postoperative complications(LTG group vs. PT-LTG group: 26.2% vs.23.6%, P=0.587), the classification of complication severity(P=0.271), and postoperative recovery was similar between two groups. Notably, the incidence of anastomotic complications of esophagojejunostomy was also comparable between the two groups(LTG group vs. PT-LTG group: 5.9% vs. 5.7%, P=0.918). The univariate and multivariate analysis confirmed that positive proximal margin [positive vs. negative: odds ratio(OR)=14.094, 95%confidence interval(95% CI): 2.639-75.260, P=0.002], rather than PT, has an impact on anastomotic complications after LTG(OR=0.945, 95% CI: 0.371-2.408, P=0.905).Conclusions: PT did not increase the surgical risk of LTG for GC/GEJC. Therefore, considering the positive effect of PT on long-term survival, the broader application of PT and LTG for GC/GEJC is supported by our findings. | Yuehong Chen Zhijing Yang Mingli Zhao Chuanjin Xu Yuxuan Zhu Huimin Zhang Huilin Huang Yanmei Peng Yanfeng Hu Tian Lin Tao Chen Hao Chen Liying Zhao Hao Liu Guoxin Li Jiang Yu Xinhua Chen | 2023 | Chinese Journal of Cancer Research2023，35，4： | 0 |
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