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| 1 | Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas显示文摘AIM To observe the drug sensitizing effect andrelated mechanisms of fas gene transduction onhuman drug-resistant gastric cancer cellSGC7901/VCR(resistant to Vincristine).METHODS The cell cycle alteration wasobserved by FACS.The sensitivity of gastriccancer cells to apoptosis was determined by invitro apoptosis assay.The drug sensitization ofcells to several anti-tumor drugs was observedby MTT assay.Immunochemical method wasused to show expression of P-gp and Topo Ⅱ ingastric cancer cells.RESULTS Comparing to SGC7901 and pBK-SGC7901/VCR,fas-SGC7901/VCR showeddecreasing G2 cells and increasing S cells,theG2 phase fraction of pBK-SGC7901/VCR wasabout 3.0 times that of fas-SGC7901/VCR,but Sphase fraction of fas-SGC7901/VCR was about1.9 times that of pBK-SGC7901/VCR,indicatingS phase arrest of fas-SGC7901/VCR.FACS alsosuggested apoptosis of fas-SGC7901/VCR,fas-SGC7901/VCR was more sensitive to apoptosisinducing agent VM-26 than pBK-SGC7901/VCR.MTT assay showed increased sensitization offas-SGC7901/VCR to DDP,MMC and 5-FU,butsame sensitization to VCR according to pBK-SGC7901/VCR.SGC7901,pBK-SGC7901/ VCRand fas-SGC7901/VCR had positively stainedTopo Ⅱ equally.P-gp staining in pBK- SGC7901/VCR was stronger than in SG07901,but there was little staining of P-gp in fas.SGC7901/VCR.CONCLUSION fas gene transduction couldreverse the MDR of human drug-resistant gastriccancer cell SGC7901/VCR to a degree,possiblybecause of higher sensitization to apoptosis anddecreased expression of P-gp. | Yin F Shi YQ Zhao WP Xiao B Miao JY Fan DM | 2000 | World Journal of Gastroenterology2000，6，5： | 24 |
| 2 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 3 | Activated CD4^+ CD25 ^+ T cells selectively kill B lymphocytes 显示文摘 | Zhao DM Thornton AM Dipaolo Rj | 2006 | Blood2006，107，： | 1 |
| 4 | Bax Reversal of multidrug resistance of gastriccancercellline27901/VCRbyBaxgenetransduction显示文摘 | Zhao QY Xiao B Fan DM | 2000 | ChinJDigest2000，20，1： | 1 |
| 5 | Effect of trehalose on PC12cells overexpressing wild-type or A53T mutantα-synuclein显示文摘 | Lan DM Liu FT Zhao J | 2012 | Neurochem Res2012，37，9： | 1 |
| 6 | Phospho- proteomic analysis of the human pituitary 显示文摘 | Beranova-Giorgianni S Zhao Y Desiderio DM | 2006 | Pituitary2006，9，2： | 1 |
| 7 | Regulation of mature T cell responses by the Wnt signaling pathway显示文摘 | Xue HH Zhao DM | 2012 | Ann NY Acad Sci2012，1247，： | 1 |
| 8 | Activated monoeytes in pefitumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1 显示文摘 | Kuang DM Zhao Q Peng C | 2009 | J Exp Med2009，206，6： | 1 |
| 9 | Location of MBL-associat-ed serine proleases binding motifs on human mannan-bindinglectin (MBL)显示文摘 | Zuo DM Cai XM Zhao N | 2010 | Protein Peptide Lett2010，17，： | 1 |
| 10 | Peritumoral neutrophils link inflammatory response to disease progression by fostering angio-genesis in hepatocellular carcinoma 显示文摘 | Kuang DM Zhao Q Wu Y | 2011 | J Hepatol2011，54，5： | 1 |
| 11 | Dose-related influence of chronic alcohol consumption on cerebral ischemia/reperfusion injury显示文摘 | Zhao H Mayhan WG Arrick DM | | 0，，： | 1 |
| 12 | Activated CD4+ CD25+T cells selectively kill B lymphocytes显示文摘 | Zhao DM Thomton AM Dipaolo RJ | | 0，，： | 1 |
| 13 | Modifiedbusulfan and cyclophosphamide conditioning regimen for allogeneic hematopoietic stem cell transplantation in the treatment of patients with hematologic malignancies显示文摘 | Zhao XF Mao XF Wan DM | 2014 | Transplant Proc2014，46，5： | 1 |
| 14 | Tumor-educated tolerogenic dendritic cells induce CD3epsilon down-regulation and apoptosis of T cells through oxygen-dependent pathways显示文摘 | Kuang DM Zhao Q Xu J | 2008 | J Immunol2008，181，5： | 1 |
| 15 | Activated CD4 + CD25 + T cells selectively kill B lymphocytes显示文摘 | Zhao DM Thornton AM Dipaolo RJ | 2006 | Blood2006，107，10： | 1 |
| 16 | ActivatedCD69 + T cells foster immune privilege by regulatingIDO expression in tumor-associated macrophages 显示文摘 | ZHAO Q KUANG DM WU Y | 2012 | J Immunol2012，188，3： | 1 |
| 17 | Early cenozoic two-phase extension and late cenozoic thermal subsidence and inversion of theBohaiBasin, northernChina显示文摘 | Allen MB Macdonald DM Zhao X | 1997 | Marine and Petroleum Geology1997，14，78： | 1 |
| 18 | Alcohol-induced exacerbation of ischemic brain injury:role of NAD(P)H oxidase显示文摘 | Zhao H Mayhan WG Arrick DM | | 0，，： | 1 |
| 19 | Identification of critical extracellular loop residues involved in alpha 1-adrenergic receptor subtype-selective antagonist binding显示文摘 | Zhao MM Hwa J Perez DM | 1996 | Mol Pharmacol1996，50，5： | 1 |
| 20 | The third extracellular loop of the beta 2-adrenergic receptor can modulate receptor/G protein affinity显示文摘 | Zhao MM Gaivin RJ Perez DM | 1998 | Mol Pharmacol1998，53，3： | 1 |
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