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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Association of farnesyl diphosphate synthase polymorphisms and response to alendronate treatment in Chinese postmenopausal women with osteoporosis显示文摘 | Liu Y Li M Zhou PR Xing XP Xia WB Xu L Liu H J Zhang ZL LiaoEY Chen DC Liu J Tao TZ Wu W | 2014 | Chinese Medical Journal2014，，4： | 16 |
| 3 | Magnetic resonance imaging of hepatocellular carcinoma induced by diethylnitrosamine in Sprague-Dawley rats显示文摘Diethylnitrosamine (DENA) is able to induce various benign and malignant liver lesions in rats with a high success rate and a low mortality rate. It provides a more appropriate model that better simulates the various lesions occurring in humans than the usual model of tumor implantations. The aim of the present study was to evaluate MRI liver examination in Sprague-Dawley(SD) rats as a routine method to detect hepatocellular carcinoma (HCC) nodules induced by DENA and to follow up their growth. METHODS:Hepatic carcinogenesis was induced in 80 male SD rats using oral DENA solution. All animals were imaged for liver tumor detection with a 1.5 Tesla magnet (Siemens Sonata,Erlangen, Germany) using correspondence scan parameters and a radio-frequency knee coil. Macroscopic examinations were performed along the axial MRI sections to evaluate magnetic resonance imaging (MRI) findings, and histopathological assessment was also performed. RESULTS:No false negative results were obtained on MR images. Hepatic tumors in 72 rats were confirmed macro-scopically and 68 rats were detected by MRI till the 20th week. The smallest and the largest nodules detected by MRI were 2 mm and 37 mm in diameter respectively. The agreement rate of MRI with macroscopic observation was 39. 1% and 97. 4% respectively for 2 mm to 5 mm and more than 5 mm nodules. CONCLUSIONS:The hepatic tumor induced by DENA provides a more representative range of tumors for imaging diagnosis and interventional treatment. MRI is the best approach for scrutinizing pathological changes of rat livers in the period of observation. | Department of Radiology, West China Hospital, Sichuan University, Chengdu 610041, China (Li X, Zhou XP and Guan YS) Department of Radiology, Ruijin Hospieal. Shanghai Second Medical University, Shanghai 200025 , China. Current address:Astra Zeneca R & D Alderley, Macclesfield SK 10 4 TG U. K. (Wang YX) | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 3 |
| 4 | Mutational analysis of the PTEN gene in gliomas: molecular and pathological correlations显示文摘 | Li YJ Hoang-Xuan K | 1999 | Int J Cancer1999，84，2： | 1 |
| 5 | Epigenetic PTEN silencing in malignant melanomas without PTEN mutation显示文摘 | Zhou XP Gimm O Hampel H | 2000 | Am J Pathol2000，157，： | 1 |
| 6 | The pathogenic meehanism of severe acute pancreatitis complicated with renal injury: a review of current knowledge显示文摘 | Zhang XP Wang L Zhou YF | 2008 | Dig Dis Sei2008，53，2： | 1 |
| 7 | Clinically applicable medels to characterize BRCA1 and BRCA2 variants of uncertain significance 显示文摘 | Spearcman AD Sweet K Zhou XP | 2008 | J Clin Oncol2008，26，33： | 1 |
| 8 | Freguent loss of PTEN expression is liked to elevated phosporylated AKT levels, but not associated with p27 and cyclin D expression, in primary epithelial ovarian carcinomas显示文摘 | Zhou XP Araki T | 2001 | Am J Pathol2001，158，6： | 1 |
| 9 | Streptozotocin induced cardiomyopathy in diabetic rats显示文摘 | Zhu XX Zhou XP Zhong XL | 1993 | Chi Med J1993，106，6： | 1 |
| 10 | Mutational analysis of the PTEN genein gliomas:molecular and pathological correlations显示文摘 | Li YJ Hoang Xuan K | 1999 | Int J Cancer1999，84，2： | 1 |
| 11 | The pathogenic mechanism of severe acute pancreatitis complicated with renal injury: a review of current knowledge显示文摘 | Zhang XP Wang L Zhou YF | 2008 | Dig Dis Sci2008，53，2： | 1 |
| 12 | Up-regulation of {gamma}-catenin compensates for the loss of {beta}-catenin in adult cardiac myocytes显示文摘 | Zhou J Qu J Yi XP | 2006 | Am J Physiol Heart Circ Physiol2006，，： | 1 |
| 13 | Clinical features and outcome ofneuro-psychiatric lupus in Chinese:analysis of 240 hospitalized patients显示文摘 | Zhou HQ Zhang FC Tian XP | 2008 | Lupus2008，17，2： | 1 |
| 14 | The pathogenic mechanism of severe acute pancreat itis complicated with renal injury:a review of current knowledge显示文摘 | Zhang XP Wang L Zhou YF | 2008 | Dig Dis Sci2008，53，2： | 1 |
| 15 | Frequent loss of PTEN expression is linked to elevated phosphorylated Akt levels, but not associated with p27 and cyclin D1 expression, in primary epithelial ovarian carcinoma显示文摘 | Kurose K Zhou XP Araki T | 2001 | Am J Pathol2001，158，9： | 1 |
| 16 | Soluble egg antigen from Schistosoma japonicum modulates the progressionof chronic progressive experimental autoimmune encephalomyelitis viaTh2-shift response显示文摘 | Zheng XP Hu XQ Zhou GY | 2008 | J Neuroimmunol2008，194，： | 1 |
| 17 | PTEN mutational spectra, expression levels, and subcellular localization in microsatellite stable and unstable colorectal cancers显示文摘 | Zhou XP Loukola A Salovaara R | 2002 | Am J Pathol2002，161，2： | 1 |
| 18 | Evaluation of antiinflammatory activity of the total flavonoids of Laggera pterodonta on acute and chronic inflammation models显示文摘 | Wu YH Zhou CX Li XP | | 0，，7： | 1 |
| 19 | Frequent loss of PTEN expression is linked to elevated phosphory lated AKT levels,but not associated with P27 and cyclin D1 expression,in primary epithelial ovarian carcinomas显示文摘 | Kurose K Zhou XP Araki T | 2001 | AM J Pathol2001，158，6： | 1 |
| 20 | Streptozotocin induced cardiomyopathy in diabetic rats显示文摘 | Zhu XX Zhou XP Zhong XL | 2003 | Chin Med J(Engl)2003，106，6： | 1 |
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