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| 1 | Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro显示文摘Dear Editor,In December 2019,a novel pneumonia caused by a previously unknown pathogen emerged in Wuhan,a city of 11 million people in central China.The initial cases were linked to exposures in a seafood market in Wuhan.1 As of January 27,2020,the Chinese authorities reported 2835 confirmed cases in China's Mainland,including 81 deaths.Additionally,19 confirmed cases were identified in Hong Kong,Macao and Taiwan,and 39 imported cases were identified in Thailand,Japan,South Korea,United States,Vietnam,Singapore,Nepal,France,Australia and Canada.The pathogen was soon identified as a novel coronavirus(2019-nCoV),which is closely related to sever acute respiratory syndrome CoV(SARS-CoV).2 Currently,there is no specific treatment against the new virus.Therefore,identifying effective antiviral agents to combat the disease is urgently needed. | Manli Wang Ruiyuan Cao Leike Zhang Xinglou Yang Jia Liu Mingyue Xu Zhengli Shi Zhihong Hu Wu Zhong Gengfu Xiao | 2020 | Cell Research2020，30，3： | 563 |
| 2 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 3 | Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein显示文摘Very recently,a novel coronavirus,2019-nCoV,emerged in Wuhan,China and then quickly spread worldwide,resulting in>17,388 confirmed cases and 361 deaths as of 3 February 2020,thus calling for the development of safe and effective therapeutics and prophylatics.1,2 Similar to severe acute respiratory syndrome(SARS)-CoV,2019-nCoV belongs to lineage B betacoronavirus,and it has the ability to utilize human angiotensin-converting enzyme 2(ACE2)as a receptor to infect human cells. | Shuai Xia Yun Zhu Meiqin Liu Qiaoshuai Lan Wei Xu Yanling Wu Tianlei Ying Shuwen Liu Zhengli Shi Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，7： | 27 |
| 4 | A new type of leak-floor flip bucket显示文摘Flip buckets are commonly used to discharge flow away from a hydraulic structure into the downstream to dissipate energy.A new leak-floor flip bucket is presented,making the ski-jump water jet a typical long-narrow nappe.Based on the model experiments and numerical simulation,the flow pattern,formation process and mechanism of the leak-floor flip bucket are studied.The results show that cross section flow shape develops from the'Y-type'to'|-type',and this is because the natural pressure difference is generated when water flows through the leak-floor area and moves transversely from both sides to the center.Different from the slit-type flip bucket with sidewall contraction,the leak-floor flit bucket makes the water jet narrow and long without high pressure on the side walls of the flip bucket.Under the same jet length condition,the maximum sidewall pressure of the slit-type is 4.67 times that of the leak-floor flip bucket.The effects of flow discharge on the jet length are less significant for the leak-floor bucket than for the slit-type bucket. | DENG Jun YANG ZhengLi TIAN Zhong ZHANG FaXing WEI WangRu YOU Xiang XU WeiLin | 2016 | Science China(Technological Sciences)2016，59，4： | 15 |
| 5 | Restricted allowable generalized quantum gates显示文摘An allowable generalized quantum gate(introduced by Long,Liu and Wang)has the form of U1 0 d kk k c U -=∑,where Uk's are unitary operators on a Hilbert space H and 1 0 1 d kkc -= |∑|≤and 1 k |c|≤(0≤k≤d-1).In this work we consider a kind of AGQGs,called restricted allowable generalized quantum gates(RAGQGs),satisfying 1 0 0 1 d kk c -= <∑||≤.Some properties of the set RAGQG(H)of all RAGQGs on H are established.Especially,we prove that the extreme points of RAGQG(H)are exactly unitary operators on H and that B(H)=R+RAGQG(H). | CAO HuaiXin LI Li CHEN ZhengLi ZHANG Ye GUO ZhiHua | 2010 | Chinese Science Bulletin2010，55，20： | 14 |
| 6 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 7 | Separation control using synthetic vortex generator jets in axial compressor cascade显示文摘在一条高速度的飞机串联风隧道进行的试验性的调查由使用表明那不稳定的流动控制合成(零集体流动) 旋涡生成器喷气能有效地改进空气动力学的表演和还原剂(或消除) 在轴的压缩机串联的流动分离。到来的流动的马赫数字直到 0.7,大多数测试用例在妈 = 0.3。发生是界面层与 70% 弦长度在被分开的 10 °。刺激频率,振幅,地点和沥青角度的角色被调查。初步的结果证明刺激振幅起一个很重要的作用,最佳的刺激地点只分离点是在上游的,并且最佳的沥青角度是 35 °。损失系数的最大的相对减小是 22.8% 。 | Xinqian Zheng Sheng Zhou Anping Hou Zhengli Jiang Daijun Ling | 2006 | Acta Mechanica Sinica2006，22，6： | 11 |
| 8 | Rapid Detection of Filoviruses by Real-time TaqMan Polymerase Chain Reaction Assays显示文摘Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 1010 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China. | Yi Huang Hongping Wei Yunpeng Wang Zhengli Shi Herve Raoul Zhiming Yuan | 2012 | Virologica Sinica2012，27，5： | 10 |
| 9 | Emerging infectious diseases associated with bat viruses显示文摘Bats play important roles as pollen disseminators and pest predators.However,recent interest has focused on their role as natural reservoirs of pathogens associated with emerging infectious diseases.Prior to the outbreak of severe acute respiratory syndrome(SARS),about 60 bat virus species had been reported.The number of identified bat viruses has dramatically increased since the initial SARS outbreak,and most are putative novel virus species or genotypes.Serious infectious diseases caused by previously identified bat viruses continue to emerge throughout in Asia,Australia,Africa and America.Intriguingly,bats infected by these different viruses seldom display clinical symptoms of illness.The pathogenesis and potential threat of bat-borne viruses to public health remains largely unknown.This review provides a brief overview of bat viruses associated with emerging human infectious diseases. | SHI ZhengLi | 2013 | Science China(Life Sciences)2013，56，8： | 10 |
| 10 | Viral Metagenomics Analysis of Planktonic Viruses in East Lake,Wuhan,China显示文摘East Lake(Lake Donghu),located in Wuhan,China,is a typical city freshwater lake that has been experiencing eutrophic conditions and algal blooming during recent years.Marine and fresh water are considered to contain a large number of viruses.However,little is known about their genetic diversity because of the limited techniques for culturing viruses.In this study,we conducted a viral metagenomic analysis using a high-throughput sequencing technique with samples collected from East Lake in Spring,Summer,Autumn,and Winter.The libraries from four samples each generated 234,669,71,837,12,820,and 34,236 contigs(>90 bp each),respectively.The genetic structure of the viral community revealed a high genetic diversity covering 23 viral families,with the majority of contigs homologous to DNA viruses,including members of Myoviridae,Podoviridae,Siphoviridae,Phycodnaviridae,and Microviridae,which infect bacteria or algae,and members of Circoviridae,which infect invertebrates and vertebrates.The highest viral genetic diversity occurred in samples collected in August,then December and June,and the least diversity in March.Most contigs have low-sequence identities with known viruses.PCR detection targeting the conserved sequences of genes(g20,psbA,psbD,and DNApol)of cyanophages further confirmed that there are novel cyanophages in the East Lake.Our viral metagenomic data provide the first preliminary understanding of the virome in one freshwater lake in China and would be helpful for novel virus discovery and the control of algal blooming in the future. | Xingyi Ge Yongquan Wu Meiniang Wang Jun Wang Lijun Wu Xinglou Yang Yuji Zhang Zhengli Shi | 2013 | Virologica Sinica2013，28，5： | 8 |
| 11 | Quantitative sufficient conditions for adiabatic approximation显示文摘Two linear In this letter, we prove the following conclusions by introducing a function Fn(t): (1) If a quantum system S with a time-dependent non-degenerate Hamiltonian H(t) is initially in the n-th eigenstate of H(0), then the state of the system at time t will remain in the n-th eigenstate of H(t) up to a multiplicative phase factor if and only if the values Fn(t) for all t are always on the circle centered at 1 with radius 1; (2) If a quantum system S with a time-dependent Hamiltonian H(t) is initially in the n-th eigenstate of H(0), then the state of the system at time t will remain-uniformly approximately in the n-th eigenstate of H(t) up to a multiplicative phase factor if and only if the values Fn(t) for all t are always outside of the circle centered at 1 with radius 1 . Moreover, some quantitative sufficient conditions for the state of the system at time t to remain-uniformly approximately in the n-th eigenstate of H(t) up to a multiplicative phase factor are established. Lastly, our results are illustrated by a spin-half particle in a rotating magnetic field. | CAO HuaiXin GUO ZhiHua CHEN ZhengLi WANG WenHu | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，7： | 7 |
| 12 | Shuanghuanglian injection downregulates nuclear factor-kappa B expression in mice with viral encephalitis显示文摘A mouse model of viral encephalitis was induced by intracranial injection of a Coxsackie virus B3 suspension. Quantitative real-time reverse transcription-PCR and western blot assay were applied to detect mRNA and protein expression of intelectin-2 and nuclear factor-kappa B in the viral encephalitis and control groups. Nuclear factor-kappa B and intelectin-2 mRNA and protein expression were significantly increased in mice with viral encephalitis. After intraperitoneal injection of Shuanghuanglian at a dose of 1.5 mg/kg for 5 successive days, intelectin-2 and nuclear factor-kappa B protein and mRNA expression were significantly decreased. To elucidate the relationship between intelectin-2 and nuclear factor-kappa B, mice with viral encephalitis were administered an intracerebral injection of 107 pfu recombinant lentivirus expressing intelectin shRNA. Both protein and mRNA levels of intelectin and nuclear factor-kappa B in brain tissue of mice were significantly decreased. Experimental findings suggest that Shuanghuanglian injection may downregulate nuclear factor-kappa B production via suppression of intelectin production, thus inhibiting inflammation associated with viral encephalitis. | Naibing Gu Ye Tian Zhengli Di Caiping Han Hui Lei Gejuan Zhang | 2012 | Neural Regeneration Research2012，7，33： | 7 |
| 13 | Operational properties and matrix representations of quantum measures显示文摘Denoted by M(A),QM(A)and SQM(A)the sets of all measures,quantum measures and subadditive quantum measures on a σ-algebra A,respectively.We observe that these sets are all positive cones in the real vector space F(A)of all real-valued functions on A and prove that M(A)is a face of SQM(A).It is proved that the product of m grade-1 measures is a grade-m measure.By combining a matrix Mμto a quantum measureμon the power set An of an n-element set X,it is proved thatμν(resp. μ⊥ν)if and only if μν M M(resp.MμMv=0).Also,it is shown that two nontrivial measuresμandνare mutually absolutely continuous if and only ifμ·ν∈QM(An).Moreover,the matrices corresponding to quantum measures are characterized. Finally,convergence of a sequence of quantum measures on An is introduced and discussed;especially,the Vitali-Hahn-Saks theorem for quantum measures is proved. | GUO ZhiHua CAO HuaiXin CHEN ZhengLi YIN JunCheng | 2011 | Chinese Science Bulletin2011，56，16： | 6 |
| 14 | Transition of axial segregation patterns in a long rotating drum显示文摘Axial segregation of a bidisperse mixture of particles in a long rotating drum is studied using the discrete element method. Simulation results show that particle interaction is responsible for axial segregation,the patterns of which are influenced by the end wall effect. Axial segregation patterns transform under competing influences of the end walls and the particle interaction forces. The two influential factors vary with various rotational speeds and end wall friction levels. The result is the transition of different axial segregation patterns: two large-particle bands at both ends, two small-particle bands at both ends, or a random segregation pattern where either a large-particle band or small-particle band may appear at either end. | Zequn Cui Yongzhi Zhao Youchuan Chen Xiao Liu Zhengli Hua Chilou Zhou Jinyang Zheng | 2014 | Particuology2014，12，2： | 6 |
| 15 | Dynamic analysis of the Wenchuan Earthquake disaster and reconstruction with 3-year remote sensing data显示文摘Earth observation is an effective technique that plays an important role in earthquake damage reduction and reconstruction.This paper introduces the results of dynamic analysis on monitoring and assessing heavily impacted areas affected by the Wenchuan Earthquake using remote sensing data acquired in the past 3 years from 2008 to 2010.Immediately after the disaster on 12 May 2008,the Chinese Academy of Sciences launched a project entitled‘Wenchuan Earthquake Disasters Monitoring and Assessment Using Remote Sensing Technology.’More than 400 images from 17 satellites and 20.2TB airborne remote sensing data were acquired to facilitate quick monitoring and evaluation of severely damaged areas in 14 counties.Results of the image analyses were forwarded on a timely basis to assist with consultative service and decisionmaking support.In subsequent years,in order to monitor the process of environmental restoration and reconstruction,airborne optical remote sensing images covering most of the severely damaged areas were again acquired in May 2009 and April 2010.These images were analyzed and compared along with images from 2008.Results were useful in support of further work on environmental protection and reconstruction in earthquake-damaged areas.Three typical areas were selected for illustrative purposes including Tangjiashan Barrier Lake,Beichuan County,and counties of Yingxiu and the new Beichuan.These results well demonstrate the importance and effectiveness of the utility of earth observation for disaster mitigation and reconstruction. | Huadong Guo Liangyun Liu Liping Lei Yanhong Wu Liwei Li Bing Zhang Zhengli Zuo Zhen Li | 2010 | International Journal of Digital Earth2010，3，4： | 5 |
| 16 | Development and Validation of a Novel Reverse Phase HPLC for Determination of Aloperine in Aloperine Tablets显示文摘Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antimflammatory actions. Methods The samples were analyzed on a ODS column with methanol-water-triethylamine (3:97:0.1 V/V) as a mobile phase, The flow rate was 1.0 mL·min^-1, and UV detection wavelength 205 nm. Results Linear regression equation was A = 1.6920C + 1.7455 (r^2 = 0.9999, n = 5) in concentratim ranging from 20 to 120μg·mL^-1. The recoveries were 101.2±1.46 % at 80 μg·mL^-1, 100.5±0.75% at 100μg·mL^-1, and 100.7±1.10% at 120 μg·mL^-1, respectively, and the precisions of aloperine within or between run were from 0.80% to 1.98% ( n = 5). The relative contents of aloperine in three lots of tablets were 101.59 ± 1.38%, 98.46 ± 0.23%, and 99.41 ± 1.09% ( n = 3).Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection. | WANGHua ZHENGLi WANGZhan ZHANGYong-qiang] WUFeng-lan | 2003 | Journal of Chinese Pharmaceutical Sciences2003，12，4： | 4 |
| 17 | Application Effect of CEMP in the Foreland Basin on the Western Edge of Ordos显示文摘Foreland basins on the western edge of Ordos are characterized by typical loess geomorphologic features. Its surface is severely cut, and its underground geological structures are complicated.Making use of the advantage of CEMP in exploration in frontland as well as successful multi-year exploration experiences in West China, we first employed the CEMP method to conduct exploration on the western edge of the Ordos basin and its surrounding areas. The practices proved that rich and reliable geological results have been achieved with the CEMP method in complicated areas covered with loess. This can provide important evidence for researching the contact relationship among basins and mountains, structural feature of target layers and predict favourable areas. | ZhengLi WangYuqing HeZhanxiang TaoDeqiang AnSixi | 2004 | Applied Geophysics2004，1，2： | 3 |
| 18 | Comprehensive interactome analysis of the spike protein of swine acute diarrhea syndrome coronavirus显示文摘Swine acute diarrhea syndrome coronavirus(SADS‐CoV)is a recently discovered coronavirus that causes severe and acute diarrhea and rapid weight loss in piglets.SADS‐CoV was reported to be capable of infecting cell lines derived from diverse species,including bats,mice,hamsters,rats,chickens,pigs,nonhuman primates,and humans,implying its high risk of cross‐species infection.However,its receptor is still unknown.In this study,the receptor‐binding domain of the SADS‐CoV spike(S)protein was purified and then subjected to affinity purification(AP)‐coupled mass spectrometry(MS)‐based proteomic analysis to identify the interactors of the SADS‐CoV S protein.Forty‐three host proteins were identified,and a Gene Ontology analysis indicated that these interactors can be grouped into categories such as“cell‐cell adhesion”,“translation”“viral transcription”,suggesting that these processes may participate in the SADS‐CoV life cycles.RNA interference‐based screening of these interactors indicated that PPIB and vimentin can affect SADS‐CoV replication.Our study provides an overarching view into the host interactome of the SADS‐CoV S protein and highlights potential targets for the development of therapeutics against SADS‐CoV. | Qingxing Wang Yun Luo Weijuan Shang Zhengli Shi Gengfu Xiao Leike Zhang | 2021 | Biosafety and Health2021，3，3： | 3 |
| 19 | Cellular immunotherapy for hematological malignancy: recent progress and future perspectives显示文摘Advancements in the field of cellular immunotherapy have accelerated in recent years and have changed the treatment landscape for a variety of hematologic malignancies.Cellular immunotherapy strategies exploit the patient’s immune system to kill cancer cells.The successful use of CD19 chimeric antigen receptor(CAR)T-cells in treating B-cell malignancies is the paradigm of this revolution,and numerous ongoing studies are investigating and extending this approach to other malignancies.However,resistance to CAR-Tcell therapy and non-durable efficacy have prevented CAR-T-cells from becoming the ultimate therapy.Because natural killer(NK)cells play an essential role in antitumor immunity,adoptively transferred allogeneic NK and CAR-modified NK cell therapy has been attempted in certain disease subgroups.Allogenic hematopoietic stem cell transplantation(allo-HSCT)is the oldest form of cellular immunotherapy and the only curative option for hematologic malignancies.Historically,the breadth of application of allo-HSCT has been limited by a lack of identical sibling donors(ISDs).However,great strides have recently been made in the success of haploidentical allografts worldwide,which enable everyone to have a donor.Haploidentical donors can achieve comparable outcomes to those of ISDs and even better outcomes in certain circumstances because of a stronger graft vs.tumor effect.Currently,novel strategies such as CAR-T or NK-based immunotherapy can be applied as a complement to allo-HSCT for curative effects,particularly in refractory cases.Here,we introduce the developments in cellular immunotherapy in hematology. | Zhengli Xu Xiaojun Huang | 2021 | Cancer Biology & Medicine2021，18，4： | 3 |
| 20 | Complex duality quantum computers acting on pure and mixed states显示文摘The aim of this paper is to establish a mathematical fundamental of complex duality quantum computers(CDQCs) acting on vector-states(pure states) and operator-states(mixed states),respectively.A CDQC consists of a complex divider,a group of quantum gates represented by unitary operators,or quantum operations represented by completely positive and trace-preserving mappings,and a complex combiner.It is proved that the divider and the combiner of a CDQC are an isometry and a contraction,respectively.It is proved that the divider and the combiner of a CDQC acting on vector-states are dual,and in the finite dimensional case,it is proved that the divider and the combiner of a CDQC acting on operator-states(matrix-states) are also dual.Lastly,the loss of an input state passing through a CDQC is measured. | CAO HuaiXin CHEN ZhengLi GUO ZhiHua REN FangGuo | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 3 |
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