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| 1 | Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing显示文摘Tracing the emergence of the first hematopoietic stem cells(HSCs)in human embryos,particularly the scarce and transient precursors thereof,is so far challenging,largely due to the technical limitations and the material rarity.Here,using single-cell RNA sequencing,we constructed the first genome-scale gene expression landscape covering the entire course of endothelial-to-HSC transition during human embryogenesis.The transcriptomically defined HSC-primed hemogenic endothelial cells(HECs)were captured at Carnegie stage(CS)12-14 in an unbiased way,showing an unambiguous feature of arterial endothelial cells(ECs)with the up-regulation of RUNX1,MYB and ANGPTL Importantly,subcategorizing CD34+CD45-ECs into a CD44+population strikingly enriched HECs by over 10-fold.We further mapped the developmental path from arterial ECs via HSC-primed HECs to hematopoietic stem progenitor cells,and revealed a distinct expression pattern of genes that were transiently over-represented upon the hemogenic fate choice of arterial ECs#including EMCN,PROCR and RUNX1T1.We also uncovered another temporally and molecularly distinct intra-embryonic HEC population,which was detected mainly at earlier CS 10 and lacked the arterial feature.Finally,we revealed the cellular components of the putative aortic niche and potential cellular interactions acting on the HSCprimed HECs.The cellular and molecular programs that underlie the generation of the first HSCs from HECs in human embryos,together with the ability to distinguish the HSC-primed HECs from others,will shed light on the strategies for the production of clinically useful HSCs from pluripotent stem cells. | Yang Zeng Jian He Zhijie Bai Zongcheng Li Yandong Gong Chen Liu Yanli Ni Junjie Du Chunyu Ma Lihong Bian Yu Lan Bing Liu | 2019 | Cell Research2019，29，11： | 14 |
| 2 | Disruption of adipocyte HIF-1α improves atherosclerosis through the inhibition of ceramide generation显示文摘Atherosclerosis is a chronic multifactorial cardiovascular disease.Western diets have been reported to affect atherosclerosis through regulating adipose function.In high cholesterol diet-fed ApoE^(−/−)mice,adipocyte HIF-1αdeficiency or direct inhibition of HIF-1αby the selective pharmacological HIF-1αinhibitor PX-478 alleviates high cholesterol diet-induced atherosclerosis by reducing adipose ceramide generation,which lowers cholesterol levels and reduces inflammatory responses,resulting in improved dyslipidemia and atherogenesis.Smpd3,the gene encoding neutral sphingomyelinase,is identified as a new target gene directly regulated by HIF-1αthat is involved in ceramide generation.Injection of lentivirus-SMPD3 in epididymal adipose tissue reverses the decrease in ceramides in adipocytes and eliminates the improvements on atherosclerosis in the adipocyte HIF-1α-deficient mice.Therefore,HIF-1αinhibition may constitute a novel approach to slow atherosclerotic progression. | Pengcheng Wang Guangyi Zeng Yu Yan Song-yang Zhang Yongqiang Dong Yangming Zhang Xingzhong Zhang Huiying Liu Zhipeng Zhang Changtao Jiang Yanli Pang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 6 |
| 3 | Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses显示文摘Human skeletal stem cells(SSCs)have been discovered in fetal and adult long bones.However,the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive.Here we map the transcriptional landscape of human limb buds and embryonic long bones at single-cell resolution to address this fundamental question.We found remarkable heterogeneity within human limb bud mesenchyme and epithelium,and aligned them along the proximal-distal and anterior-posterior axes using known marker genes.Osteo-chondrogenic progenitors first appeared in the core limb bud mesenchyme,which give rise to multiple populations of stem/progenitor cells in embryonic long bones undergoing endochondral ossification.Importantly,a perichondrial embryonic skeletal stem/progenitor cell(eSSPC)subset was identified,which could self-renew and generate the osteochondral lineage cells,but not adipocytes or hematopoietic stroma.eSSPCs are marked by the adhesion molecule CADM1 and highly enriched with FOXP1/2 transcriptional network.Interestingly,neural crest-derived cells with similar phenotypic markers and transcriptional networks were also found in the sagittal suture of human embryonic calvaria.Taken together,this study revealed the cellular heterogeneity and lineage hierarchy during human embryonic skeletogenesis,and identified distinct skeletal stem/progenitor cells that orchestrate endochondral and intramembranous ossification. | Jian He Jing Yan Jianfang Wang Liangyu Zhao Qian Xin Yang Zeng Yuxi Sun Han Zhang Zhijie Bai Zongcheng Li Yanli Ni Yandong Gong Yunqiao Li Han He Zhilei Bian Yu Lan Chunyu Ma Lihong Bian Heng Zhu Bing Liu Rui Yue | 2021 | Cell Research2021，31，7： | 5 |
| 4 | Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells显示文摘Whereas the critical roles of innate lymphoid cells(ILCs)in adult are increasingly appreciated,their developmental hierarchy in early human fetus remains largely elusive.In this study,we sorted human hematopoietic stem/progenitor cells,lymphoid progenitors,putative ILC progenitor/precursors and mature ILCs in the fetal hematopoietic,lymphoid and non-lymphoid tissues,from 8 to 12 post-conception weeks,for single-cell RNA-sequencing,followed by computational analysis and functional validation at bulk and single-cell levels.We delineated the early phase of ILC lineage commitment from hematopoietic stem/progenitor cells,which mainly occurred in fetal liver and intestine.We further unveiled interleukin-3 receptor as a surface marker for the lymphoid progenitors in fetal liver with T,B,ILC and myeloid potentials,while IL-3RA–lymphoid progenitors were predominantly B-lineage committed.Notably,we determined the heterogeneity and tissue distribution of each ILC subpopulation,revealing the proliferating characteristics shared by the precursors of each ILC subtype.Additionally,a novel unconventional ILC2 subpopulation(CRTH2–CCR9+ILC2)was identified in fetal thymus.Taken together,our study illuminates the precise cellular and molecular features underlying the stepwise formation of human fetal ILC hierarchy with remarkable spatiotemporal heterogeneity. | Chen Liu Yandong Gong Han Zhang Hua Yang Yang Zeng Zhilei Bian Qian Xin Zhijie Bai Man Zhang Jian He Jing Yan Jie Zhou Zongcheng Li Yanli Ni Aiqing Wen Yu Lan Hongbo Hu Bing Liu | 2021 | Cell Research2021，31，10： | 2 |
| 5 | Heterogeneity in endothelial cells and widespread venous arterialization during early vascular development in mammals显示文摘Arteriogenesis rather than unspecialized capillary expansion is critical for restoring effective circulation to compromised tissues in patients.Deciphering the origin and specification of arterial endothelial cells during embryonic development will shed light on the understanding of adult arteriogenesis.However,during early embryonic angiogenesis,the process of endothelial diversification and molecular events underlying arteriovenous fate settling remain largely unresolved in mammals.Here,we constructed the single-cell transcriptomic Iandscape of vascular endothelial cells(VECs)during the time window for the occurrenee of key vasculogenic and angiogenic events in both mouse and human embryos.We uncovered two distinct arterial VEC types,the major artery VECs and arterial plexus VECs,and un expectedly diverge nt arteriove nous characteristics among VECs that are located in morphologically undistinguishable vascular plexus intra-embryonically.Using computational prediction and further lineage tracing of venous-featured VECs with a newly developed Nr2f2^(CrexER) | Siyuan Hou Zongcheng Li Ji Dong Yun Gao Zhilin Chang Xiaochen Ding Shuaili Li Yunqiao Li Yang Zeng Qian Xin Baihan Wang Yanli Ni Xiaowei Ning Yuqiong Hu Xiaoying Fan Yu Hou Xianlong Li Lu Wen Bin Zhou Bing Liu Fuchou Tang Yu Lan | 2022 | Cell Research2022，32，4： | 2 |
| 6 | Synthesis of Microporous Nitrogen-Rich Covalent-Organic Framework and Its Application in CO_(2) Capture显示文摘An imine-based nitrogen-rich covalent-organic framework(COF)was successfully synthesized using two tri-angular building units under solvothermal reaction condition.The gas adsorption properties of the obtained micro-porous nitrogen-rich COF were investigated.The results indicated that the activated COF material presented good up take capabilities of CO_(2) and CH_(4) at 61.2 and 43.4 cm^(3)·g^(−1)at 1 atm and 273 K,respectively,showing its applica-tion potential in selective gas capture and separation. | Qiang Gao Linyi Bai Xiaojing Zhang Peng Wang Peizhou Li Yongfei Zeng Ruqiang Zou Yanli Zhao | 2015 | Chinese Journal of Chemistry2015，33，1： | 2 |
| 7 | Effects of Planting Mode on Growth, Yield and Quality of Mini-watermelon under Soilless Cultivation显示文摘[Objective]The paper was to screen out planting mode suitable for mini-watermelon in spring greenhouses under soilless cultivation.[Method]The effects of single row planting with one main vine and one lateral vein,double row planting with one main vine and one lateral vein,and double row planting with two lateral veins on the growth period,growth,fruit quality and yield of mini-watermelon were studied.[Result]The planting mode of single row planting with one main vine and one lateral vein was adopted.The earliest date of harvest was May 28;the fruit-setting rate was 106.3%;and the single fruit weight was 1.33 kg.The lowest abnormal fruit rate was 0.73%;the highest yield was 54465.45 kg/hm^2,and the central sugar content was the highest of 12.95%.[Conclusion]Single row planting mode under soilless cultivation has certain demonstration and popularization prospect. | Zhang Ying Ma Chao Zeng Jianbo Zhu Li Chen Yanli Li Yunfei Li Jing You Xuesong | 2019 | Plant Diseases and Pests2019，10，4： | 1 |
| 8 | Comparative Test on New Small Watermelon Varieties with Red Flesh under Soilless Culture Conditions显示文摘In order to screen out the small watermelon varieties with red flesh suitable for soilless cultivation and to meet people's requirements of high-quality watermelons,comparative tests were conducted on 6 small watermelon varieties with red flesh under soilless culture conditions in 2018.Based on the data of field traits,yield traits,quality traits and commercial traits,the integrated results showed that ‘Chuanqi 2’ had good quality and high yield,and the overall performance was the best. | Xuesong YOU Jianbo ZENG Li ZHU Chao MA Yanli CHEN Yunfei LI Ting LI Ying ZHANG | 2018 | Agricultural Biotechnology2018，7，6： | 1 |
| 9 | Predicting sustained viral response to hepatitis C using a rapid and simple IL28B rs8099917 genotyping assay显示文摘 | LI Wei ZENG Yanli WANG Junjie | 2012 | Antiviral Research2012，94，1： | 1 |
| 10 | Computational studies of σ-type weak interactions between NCO/NCS radicals and XY(X = H,Cl;Y = F,Cl,and Br)显示文摘The triatomic radicals NCO and NCS are of interest in atmospheric chemistry,and both the ends of these radicals can potentially serve as electron donors during the formation of σ-type hydrogen/halogen bonds with electron acceptors XY(X = H,Cl;Y = F,Cl,and Br).The geometries of the weakly bonded systems NCO/NCS···XY were determined at the MP2/aug-cc-pVDZ level of calculation.The results obtained indicate that the geometries in which the hydrogen/halogen atom is bonded at the N atom are more stable than those where it is bonded at the O/S atom,and that it is the molecular electrostatic potential(MEP)-not the electronegativity-that determines the stability of the hydrogen/halogen bond.For the same electron donor(N or O/S) in the triatomic radical and the same X atom in XY,the bond strength decreases in the order Y = F > Cl > Br.In the hydrogen/halogen bond formation process for all of the complexes studied in this work,transfer of spin electron density from the electron donor to the electron acceptor is negligible,but spin density rearranges within the triatomic radicals,being transferred to the terminal atom not interacting with XY. | LI XiaoYan ZENG YanLi ZHANG XueYing ZHENG ShiJun MENG LingPeng | 2012 | Science China Chemistry2012，55，7： | 1 |
| 11 | A General One‐Pot Strategy for the Synthesis of High‐Performance Transparent‐Conducting‐Oxide Nanocrystal Inks for All‐Solution‐Processed Devices显示文摘 | Jizhong Song Sergei A. Kulinich Jianhai Li Yanli Liu Haibo Zeng | 2015 | Angew. Chem. Int. Ed2015，，： | 1 |
| 12 | Influence of the Li???π Interaction on the H/X???π Interactions in HOLi???C6H6???HOX/XOH (X=F, Cl, Br, I) Complexes显示文摘 | Yanli Zeng Wenjie Wu Xiaoyan Li Shijun Zheng Lingpeng Meng | 2013 | ChemPhysChem2013，，： | 1 |
| 13 | Theoretical Studies on the Isomerizations of CH3 NO2 显示文摘 | YANG Lijuan LI Xiaoyan ZENG Yanli | 2009 | Chin J Chem2009，27，6： | 1 |
| 14 | Characteristics of alveolar macrophages in bronchioalveolar lavage fluids from active tuberculosis patients identified by single-cell RNA sequencing显示文摘Tuberculosis(TB),is an infectious disease caused by Mycobacterium tuberculosis(M.tuberculosis),and presents with high morbidity and mortality.Alveolar macrophages play an important role in TB pathogenesis although there is heterogeneity and functional plasticity.This study aimed to show the characteristics of alveolar macrophages from bronchioalveolar lavage fluid(BALF)in active TB patients.Single-cell RNA sequencing(scRNA-seq)was performed on BALF cells from three patients with active TB and additional scRNA-seq data from three healthy adults were established as controls.Transcriptional profiles were analyzed and compared by differential gene expression and functional enrichment analysis.We applied pseudo-temporal trajectory analysis to investigate correlations and heterogeneity within alveolar macrophage subclusters.Alveolar macrophages from active TB patients at the single-cell resolution are described.We found that TB patients have higher cellular percentages in five macrophage subclusters.Alveolar macrophage subclusters with increased percentages were involved in inflammatory signaling pathways as well as the basic macrophage functions.The TB-increased alveolar macrophage subclusters might be derived from M1-like polarization state,before switching to an M2-like polarization state with the development of M.tuberculosis infection.Cell-cell communications of alveolar macrophages also increased and enhanced in active TB patients.Overall,our study demonstrated the characteristics of alveolar macrophages from BALF in active TB patients by using scRNA-seq. | Qianqian Chen Chunmei Hu Wei Lu Tianxing Hang Yan Shao Cheng Chen Yanli Wang Nan Li Linling Jin Wei Wu Hong Wang Xiaoning Zeng Weiping Xie | 2022 | The Journal of Biomedical Research2022，36，3： | 1 |
| 15 | ZnO-Based Transparent Conductive Thin Films: Doping, Performance, and Processing显示文摘 | Yanli Liu Yufang Li Haibo Zeng Xijin Xu | 2013 | Journal of Nanomaterials2013，，： | 1 |
| 16 | Study on the reaction of 3-methyl-2-butenal and 3-methylbutanal with Cl atoms: kinetics and reaction mechanism显示文摘The reaction of Cl atoms with two C5aldehydes(3-methyl-2-butenal and 3-methylbutanal)were investigated by proton-transfer-reaction mass spectrum(PTR-MS)using smog chamber at 298±1 K and 760 Torr.A relative rate method was used to determine the rate constants of the title reactions with m-xylene and trans-2-butene as reference compounds:(3.04±0.18)×10^(-10)and(2.07±0.14)×10^(-10)cm^(3)/(molecule·sec)for 3-methyl-2-butenal and 3-methylbutanal,respectively.Additionally,the gas-phase products were also identified by PTR-MS,and the possible reaction mechanisms were proposed basing on the identified products.The detected gas-phase products are similar for two C5aldehydes reactions,mainly including small molecules of aldehydes,ketones and chlorinated aldehyde compounds.The atmospheric lifetimes(τ)calculated for 3-methyl-2-butenal(τ=7.0 hr,marine boundary layer(MBL))and 3-methylbutanal(τ=10.3 hr,MBL)according to the obtained rate constants.The results indicate that Cl atoms at MBL are competitive with OH radicals for the degradation contribution of C5aldehyde compounds. | Bo Shi Weigang Wang Cici Fan Yuchan Zhang Zheng Sun Yanli Zeng Maofa Ge | 2022 | Journal of Environmental Sciences2022，34，6： | 0 |
| 17 | Implementation Status and Strategy of Undergraduate Tutorial System in the Context of Academy System显示文摘The cooperative education mode of academy system and undergraduate tutorial system is an important reform mode for cultivating top-notch innovative talents in colleges and universities.However,there is still room for improvement in the operation of tutorial system.For example,the lack of synergy in the academy system leads to problems such as insufficient understanding between teachers and students and limited guidance effect.Therefore,this study analyzes the implementation status of tutorial system,and puts forward the corresponding strategies for the existing problems. | Jie ZHU Liping ZENG Chengcheng SI Yanli CHEN Guopeng ZHU | 2023 | Asian Agricultural Research2023，15，11： | 0 |
| 18 | Screening of Red-flesh Small Watermelon Varieties for Substrate Cultivation in Spring Greenhouses显示文摘[Objectives] This study was conducted to screen small watermelon varieties suitable for spring greenhouses. [Methods] The field traits, yield traits, quality traits and commodity of 6 new red-flesh watermelon varieties were compared. [Results] 'Jingmei 3 K' and 'Sumeng No.5' had obvious advantages in quality traits, yield traits, and commodity traits. The yield reached 62 163.3 and 63 733.5 kg/hm2, respectively;the single melon weights reached 2.16 and 2.21 kg, respectively;and the central sugar content reached 13.2% and 13.1%, respectively. Meanwhile, the juice contents were high, and the taste was sweet and refreshing. They are suitable for planting under the condition of substrate cultivation technology. [Conclusions] This study provides a theoretical basis for large-area substrate cultivation and production. | Chao MA Jianbo ZENG Shengqi MU Ying ZHANG Yanli CHEN Ting LI Xuesong YOU | 2021 | Agricultural Biotechnology2021，10，1： | 0 |
| 19 | Effects of Different Amino Acid Fertilizers on Growth,Development and Quality of Mini Watermelon under Substrate Cultivation显示文摘In order to select suitable mini watermelon varieties for spring greenhouse cultivation under substrate cultivation,the field character,yield character,quality character and trade trait of six new mini watermelon varieties with red flesh were compared in the test.The results showed that mini watermelon applied with alanine and arginine had obvious advantages in quality character,yield character and trade trait.The yields reached 3876.8 and 44120.20 kg/667m2;the weight of single melon reached 1.98 and 2.10 kg;and the central sugar content reached 13.34%and 13.25%,respectively.Meantime,mini watermelon was juicy,sweet,tasty and refreshing,and was suitable for planting under substrate cultivation. | Zeng Jianbo Ma Chao Zhang Ying Li Ting Hu Xiaoyi You Xuesong Chen Yanli | 2021 | Plant Diseases and Pests2021，12，1： | 0 |
| 20 | Effects of Different Planting Densities on Growth, Yield and Quality of Mini-watermelon under Soilless Cultivation显示文摘[Objective]The paper was to study the effects of different plant densities on growth,yield and quality of mini-watermelon under soilless cultivation.[Method]Five planting densities were designed under double row planting with one main vine and one lateral vein in soilless cultivation,including 5.25×10^4,3.90×10^4,3.15×10^4,2.70×10^4 and 2.25×10^4 plants/hm^2.[Conclusion]Under the planting density of 3.15×10^4 plants/hm^2,the yield of mini-watermelon was the highest of 58.719 t/hm^2;the yield of plot was the highest of 260.97 kg;the central sugar content was the highest of 13.20%;the earliest date of harvest was June 1,ranking second;the fruit setting rate was 108.9%;the single fruit weight was 1.72 kg;and the malformed fruit rate was 0.48%.[Conclusion]Under soilless cultivation,3.15×10^4 plants/hm^2 is the best planting density. | Ma Chao Zeng Jianbo Zhang Ying Chen Yanli Li Ting You Xuesong | 2019 | Plant Diseases and Pests2019，10，5： | 0 |
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