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| 1 | SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA显示文摘HBV infection initiates hepatitis B and promotes liver cirrhosis and hepatocellular carcinoma.IFN-α is commonly used in hepatitis B therapy,but how it inhibits HBV is not fully understood.We screened 285 human interferon-stimulated genes(ISGs)for anti-HBV activity using a cell-based assay,which revealed several anti-HBV ISGs.Among these ISGs,SAMD4A was the strongest suppressor of HBV replication.We found the binding site of SAMD4A in HBV RNA,which was a previously unidentified Smaug recognition region(SRE)sequence conserved in HBV variants.SAMD4A binds to the SRE site in viral RNA to trigger its degradation.The SAM domain in SAMD4A is critical for RNA binding and the C-terminal domain of SAMD4A is required for SAMD4A anti-HBV function.Human SAMD4B is a homolog of human SAMD4A but is not an ISG,and the murine genome encodes SAMD4.All these SAMD4 proteins suppressed HBV replication when overexpressed in vitro and in vivo.We also showed that knocking out the Samd4 gene in hepatocytes led to a higher level of HBV replication in mice and AAV-delivered SAMD4A expression reduced the virus titer in HBVproducing transgenic mice.In addition,a database analysis revealed a negative correlation between the levels of SAMD4A/B and HBV in patients.Our data suggest that SAMD4A is an important anti-HBV ISG for use in IFN therapy of hepatitis B and that the levels of SAMD4A/B expression are related to HBV sensitivity in humans. | Yuze Wang Xinrui Fan Yunlong Song Yifei Liu Ruixin Liu Jianfeng Wu Xiaoling Li Quan Yuan Guo Fu Ningshao Xia Jiahuai Han | 2021 | Cellular & Molecular Immunology2021，18，4： | 1 |
| 2 | Impact of dietary supplementation of b-hydroxybutyric acid on performance,nutrient digestibility,organ development and serum stress indicators in early-weaned goat kids显示文摘The objective of this study was to determine the effect of dietary supplementation of b-hydroxybutyric acid(BHBA)on performance,nutrient digestibility,organ development,and serum composition in earlyweaned goat kids.Sixty-four goat kids at 30 d of age were assigned to 4 treatments in a completely randomized design:1)control(basal diet);2)low(basal diet with 3 g/d per animal BHBA);3)medium(basal diet with 6 g/d per animal BHBA;and 4)high(basal diet with 9 g/d per animal BHBA).Subsequently,48(6 kids per treatment)goat kids were randomly selected and slaughtered at 60 and 90 d of age.Compared with the control group,BHBA at low and high doses increased body weight(P<0.05),average daily gain(P<0.01),and average daily starter intake(P<0.01).The BHBA improved organ development,especially at the lowest dose(P<0.01).The digestibility of dry matter and crude protein increased with age(P<0.05).However,BHBA did not affect nutrient digestibility.Compared with the control group,serum ceruloplasmin increased(P<0.05)with high BHBA level at 90 d of age.However,the serum creatinine(P<0.05)increased over time but was not affected by BHBA.The serum total antioxidant capacity and superoxide dismutase decreased with the high dose of BHBA at 90 d of age(P<0.01).In contrast,the serum glutathione peroxidase and malondialdehyde increased with the high doses of BHBA(P<0.01).Overall,low doses of BHBA were positive for growth performance,organ development,and health status against weaning stress.Whereas high doses of BHBA in the long term could negatively affect antioxidant status. | Mahmoud M.Abdelsattar Einar Vargas-Bello-Perez Yimin Zhuang Yuze Fu Naifeng Zhang | 2022 | Animal Nutrition2022，，2： | 1 |
| 3 | Tuning the magnetic anisotropy in LSMO manganite films through non-magnetic nanoparticles显示文摘We report the observation of anomalous magnetic anisotropy driven by nonmagnetic ZrO2 nanoparticles in epitaxial La2/3Sr1/3MnO (LSMO) films grown on LaAlO3 (LAO) substrates. The compressive epitaxial strain imposed by the lattice mismatch of substrate and film is tuned by the density of ZrO2 nanoparticles embedded in the film matrix and affects the magnetic anisotropy as well as the magnetotransport properties. Epitaxial 54 nm thick LSMO thin films with different concentrations of ZrO2 nanoparticles demonstrate anisotropic hysteresis loops concomitant with anisotropic magnetotresistance behavior. The biaxial epitaxial strain, induced by the substrate/film lattice parameter mismatch is partially relaxed by increasing the density of precipitates and they serve as a tuning parameter for the strain state. We interpret our results by a strain-induced interplay of impurity scattering, weak localization and magnetic domain structure. | Yuze Gao Jincang Zhang Xinwen Fu Guixin Cao Hanns-Ulrich Habermeier | 2013 | Progress in Natural Science:Materials International2013，23，2： | 0 |
| 4 | Transcriptomic and metabolomic insights into the roles of exogenous b-hydroxybutyrate acid for the development of rumen epithelium in young goats显示文摘Beta-hydroxybutyric acid(BHBA),as one of the main metabolic ketones in the rumen epithelium,plays critical roles in cellular growth and metabolism.The ketogenic capacity is associated with the maturation of rumen in young ruminants,and the exogenous BHBA in diet may promote the rumen development.However,the effects of exogenous BHBA on rumen remain unknown.This is the first study to investigate the mechanisms of BHBA on gene expression and metabolism of rumen epithelium using young goats as a model through multi-omics techniques.Thirty-two young goats were divided into control,low dose,middle dose,and high dose groups by supplementation of BHBA in starter(0,3,6,and 9 g/day,respectively).Results demonstrated the dietary of BHBA promoted the growth performance of young goats and increased width and length of the rumen papilla(P<0.05).Hub genes in host transcriptome that were positively related to rumen characteristics and BHBA concentration were identified.Several upregulated hub genes including NDUFC1,NDUFB4,NDUFB10,NDUFA11 and NDUFA1 were enriched in the gene ontology(GO)pathway of nicotinamide adenine dinucleotide(NADH)dehydrogenase(ubiquinone)activity,while ATP5ME,ATP5PO and ATP5PF were associated with ATP synthesis.RT-PCR revealed the expression of genes(HMGCS2,BDH1,SLC16A3,etc.)associated with lipolysis increased significantly by BHBA supplementation(P<0.05).Metabolomics indicated that some metabolites such as glucose,palmitic acid,cortisol and capric acid were also increased(P<0.05).This study revealed that BHBA promoted rumen development through altering NADH balance and accelerating lipid metabolism,which provides a theoretical guidance for the strategies of gastrointestinal health and development of young ruminants. | Yimin Zhuang Jianmin Chai Mahmoud M.Abdelsattar Yuze Fu Naifeng Zhang | 2023 | Animal Nutrition2023，，4： | 0 |
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