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| 1 | Ethylene-induced microtubule reorientation is essential for fast inhibition of root elongation in Arabidopsis显示文摘Microtubule reorientation is a long-standing observation that has been implicated in regulating the inhibitory effect of ethylene on axial elongation of plant cells. However, the signaling mechanism underlying ethylene-induced microtubule reorientation has remained elusive. Here, we reveal, by live confocal imaging and kinetic root elongation assays, that the time courses of ethylene-induced microtubule reorientation and root elongation inhibition are highly correlated, and that microtubule reorientation is required for the full responsiveness of root elongation to ethylene treatment. Our genetic analysis demonstrated that the effect of ethylene on microtubule orientation and root elongation is mainly transduced through the canonical linear ethylene signaling pathway. By using pharmacological and genetic analyses, we demonstrate further that the TIR1/AFBs-Aux/IAAs-ARFs auxin signaling pathway, but not the ABP1-ROP6-RIC1 auxin signaling branch, is essential for ethylene-induced microtubule reorientation and root elongation inhibition. Together, these findings offer evidence for the functional significance and elucidate the signaling mechanism for ethylene-induced microtubule reorientation in fast root elongation inhibition in Arabidopsis. | Yichuan Wang Yusi Ji Ying Fu Hongwei Guo | 2018 | Journal of Integrative Plant Biology2018，60，9： | 4 |
| 2 | H3K4me3 epigenomic landscape derived from ChIP-Seq of 1000 mouse early embryonic cells显示文摘 | Jie Shen Dongqing Jiang Yusi Fu Xinglong Wu Hongshan Guo Binxiao Feng Yuhong Pang Aaron M Streets Fuchou Tang Yanyi Huang | 2015 | Cell Research2015，25，1： | 4 |
| 3 | On the complexity of ω-pushdown automata显示文摘Finite automata over infinite words(called ω-automata) play an important role in the automatatheoretic approach to system verification. Different types of ω-automata differ in their succinctness and complexity of their emptiness problems, as a result, theory of ω-automata has received considerable research attention.Pushdown automata over infinite words(called ω-PDAs), a generalization of ω-automata, are a natural model of recursive programs. Our goal in this paper is to conduct a relatively complete investigation on the complexity of the emptiness problems for variants of ω-PDAs. For this purpose, we consider ω-PDAs of five standard acceptance types: B¨uchi, Parity, Rabin, Streett and Muller acceptances. Based on the transformation for ω-automata and the efficient algorithm proposed by Esparza et al. in CAV'00 for verifying the emptiness problem of ω-PDAs with B¨uchi acceptance, it is trivial to check the emptiness problem of other ω-PDAs. However, this naive approach is not optimal. In this paper, we propose novel algorithms for the emptiness problem of ω-PDAs based on the observations of the structure of accepting runs. Our algorithms outperform algorithms that go through B¨uchi PDAs. In particular, the space complexity of the algorithm for Streett acceptance that goes through B¨uchi acceptance is exponential, while ours is polynomial. The algorithm for Parity acceptance that goes through B¨uchi acceptance is in O(k3n2m) time and O(k2nm) space, while ours is in O(kn2m) time and O(nm) space, where n(resp. m and k) is the number of control states(resp. transitions and index). Finally,we show that our algorithms yield a better solution for the pushdown model checking problem against linear temporal logic with fairness. | Yusi LEI Fu SONG Wanwei LIU Min ZHANG | 2017 | Science China(Information Sciences)2017，60，11： | 0 |
| 4 | In-situ synthesis of quantum dots in the nucleus of live cells显示文摘The cell nucleus is the main site for the storage and replication of genetic material,and the synthesis of substances in the nucleus is rhythmic,regular and strictly regulated by physiological processes.However,whether exogenous substances,such as nanoparticles,can be synthesized in situ in the nucleus of live cells has not been reported.Here,we have achieved in-situ synthesis of CdSx Se1−x quantum dots(QDs)in the nucleus by regulation of the glutathione(GSH)metabolic pathway.High enrichment of GSH in the nucleus can be accomplished by the addition of GSH with the help of the Bcl-2 protein.Then,high-valence Se is reduced to low-valence Se by glutathione-reductase-catalyzed GSH,and interacts with the Cd precursor formed through Cd and thiol-rich proteins,eventually generating QDs in the nucleus.Our work contributes to a new understanding of the syntheses of substances in the cell nucleus and wi l l pave the way for the development of advanced‘supercells’. | Yusi Hu Zhi-Gang Wang Haohao Fu Chuanzheng Zhou Wensheng Cai Xueguang Shao Shu-Lin Liu Dai-Wen Pang | 2024 | National Science Review2024，11，3： | 0 |
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