| 1 | 3D microfluidic gradient generator for combination antimicrobial susceptibility testing显示文摘Microfluidic concentration gradient generators(µ-CGGs)have been utilized to identify optimal drug compositions through antimicrobial susceptibility testing(AST)for the treatment of antimicrobial-resistant(AMR)infections.Conventionalµ-CGGs fabricated via photolithography-based micromachining processes,however,are fundamentally limited to two-dimensional fluidic routing,such that only two distinct antimicrobial drugs can be tested at once.This work addresses this limitation by employing Multijet-3D-printed microchannel networks capable of fluidic routing in three dimensions to generate symmetric multidrug concentration gradients.The three-fluid gradient generation characteristics of the fabricated 3Dµ-CGG prototype were quantified through both theoretical simulations and experimental validations.Furthermore,the antimicrobial effects of three highly clinically relevant antibiotic drugs,tetracycline,ciprofloxacin,and amikacin,were evaluated via experimental single-antibiotic minimum inhibitory concentration(MIC)and pairwise and three-way antibiotic combination drug screening(CDS)studies against model antibiotic-resistant Escherichia coli bacteria.As such,this 3Dµ-CGG platform has great potential to enable expedited combination AST screening for various biomedical and diagnostic applications. | Eric Sweet Brenda Yang Joshua Chen Reed Vickerman Yujui Lin Alison Long Eric Jacobs Tinglin Wu Camille Mercier Ryan Jew Yash Attal Siyang Liu Andrew Chang Liwei Lin | 2020 | Microsystems & Nanoengineering2020，6，1： | 1 |
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