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| 1 | Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy显示文摘An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection. | FAN XiaoJun ZHENG Bo FU YueJun SUN Yi LIANG AiHua | 2008 | Chinese Science Bulletin2008，53，12： | 7 |
| 2 | Volatile Compounds in the Flowers of Freesia Parental Species and Hybrids显示文摘几个世纪,小苍兰属植物是在养花工业的最重要的庄稼之一。这里,从双小苍兰属植物的全部花收集的水空间样品测定一,三四倍体小苍兰属植物栽培变种的三四倍体小苍兰属植物栽培变种,和种间染种用气体被分析用色层法分离结合了质量选择察觉者。总共, 75 不同混合物被识别。混合物主要是萜烯,烃,白酒,脂肪酸酉旨和芳香的班混合物。在这些之中,沉香醇除了无味的白四倍体 F 从所有有香味的花被检测。混合一。在 F 之间的沉香醇的稳定的继承。混合一 and 他们的 F1 子孙被观察。基于现在的分析,在小苍兰属植物和沉香醇的芳香之间的关系被讨论。 | Yang Fu Xiang Gao Yiqun Xue Yuejun Hui Fengqing Chen Quanping Su Li Wang | 2007 | Journal of Integrative Plant Biology2007，49，12： | 4 |
| 3 | Interplanting Patterns of Cash Crops under Forest显示文摘At the same time of tending forests,it is necessary to explore the under-forest economic development mode,bring into play production function of economic plants,establish cultivation mode of edible fungus,wild vegetables and medicinal plants under forest,to obtain certain economic benefit in short period. This paper made a preliminary study on significance and existing problems of the under-forest economy,and came up with several interplanting patterns,in the hope of providing a comprehensive operation and cultivation approach for developing the under-forest economy. | Renjie DU Yuejun QU Hu JIN Haibo JIANG Yunchang DU Dongyue ZHOU Jing FU Ping SHI | 2014 | Asian Agricultural Research2014，6，11： | 1 |
| 4 | Integrating electromagnetic surface and antenna array for reflection suppression and excellent radiation显示文摘The electromagnetic surface antenna array(EMSAA)has been proposed for obtaining reflection suppression and excellent radiation simultaneously.The antenna with rectangular radiation patch is used to design anisotropic electromagnetic surface.Preternatural reflection characteristics of the element antenna can be tailored depending on the incident polarizations.EMSAA can be constructed by using single structured element antenna with 90° rotation and orthometric arrangement.This orthometric arrangement of EMSAA is helpful to achieve reflection suppression and excellent radiation.The simulated results show that the reflection of EMSAA is suppressed from 5.0 GHz to 8.0 GHz with peak reduction of 12.3 dB.The linear-and circular-polarized radiation properties of EMSAA are obtained and the maximum gain is 14.3 dBi.The measured results are consistent with the simulation results.The results demonstrate that the reflection suppression and excellent radiation are achieved simultaneously.Such design of EMSAA will open the path for integrating antenna fields and electromagnetic surface(EMS)fields. | ZHENG Yuejun DING Liang CHEN Qiang GUO Min FU Yunqi | 2021 | Journal of Systems Engineering and Electronics2021，32，3： | 1 |
| 5 | Electric-controlled metasurface antenna array with ultra-wideband frequency reconfigurable reflection suppression显示文摘The electric-controlled metasurface antenna array(ECMSAA)with ultra-wideband frequency reconfigurable reflection suppression is proposed and realized.Firstly,an electriccontrolled metasurface with ultra-wideband frequency reconfigurable in-phase reflection characteristics is designed.The element of the ECMSAA is constructed by loading the single electric-controlled metasurface unit on the conventional patch antenna element.The radiation properties of the conventional patch antenna and the reflection performance of electric-controlled metasurface are maintained when the antenna and the metasurface are integrated.Thus,the ECMSAA elements have excellent radiation properties and ultra-wideband frequency reconfigurable in-phase reflection characteristics simultaneously.To take a further step,a 6×10 ECMSAA is realized based on the designed metasurface antenna element.Simulated and measured results prove that the reflection of the ECMSAA is dynamically suppressed in the P and L bands.Meanwhile,high-gain and multi-polarization radiation properties of the ECMSAA are achieved.This design method not only realizes the frequency reconfigurable reflection suppression of the antenna array in the ultra-wide frequency band but also provides a way to develop an intelligent low-scattering antenna. | ZHENG Yuejun CHEN Qiang DING Liang YUAN Fang FU Yunqi | 2023 | Journal of Systems Engineering and Electronics2023，34，6： | 0 |
| 6 | The microRNA pathway activation in insect cell model upon Autographa californica multiple nucleopolyhedrovirus infection显示文摘Background:The immune system of insects exerts fundamentally different antiviral mechanisms than mammals.MicroRNAs(miRNAs)play vital roles in developing insect antiviral immunity.MiRNAs expression profiles of insects changed significantly during baculovirus infection.Methods:Differential expression profiles of miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing(NGS)and RT-qPCR during Autographa californica multiple nucleopolyhedrovirus(AcMNPV)infection.The transcription levels of genes were detected by RT-qPCR.The 50%tissue culture infective dose(TCID_(50))endpoint dilution assay was used to determine the proliferation of progeny virus.Results:NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,and 10 of them were significantly up-or down-regulated.Though RT-qPCR analysis,we observed the similar trends for the expression patterns of significantly differentially expressed miRNAs from NGS.Moreover,the transcription levels of core genes,Exportin5,Dicer1,and Argonaute1,in miRNA biogenesis pathways were significantly increased after AcMNPV infection.For five selected miRNAs,miR-34-5p could regulate the proliferation of baculovirus progeny virus and energy metabolism.Conclusion:The miRNAs biogenesis pathway in Sf9 cells plays an important role and may be stimulated to resist AcMNPV infection.This work provides evidence for the molecular mechanism of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology. | QIAOJIN JIA YUEJUN FU | 2023 | BIOCELL2023，47，3： | 0 |
| 7 | A novel domain-duplicated SlitFAR3 gene involved in sex pheromone biosynthesis in Spodoptera litura显示文摘Fatty acyl reductases(FARs)are key enzymes that participate in sex pheromone biosynthesis by reducing fatty acids to fatty alcohols.Lepidoptera typically harbor numerous FAR gene family members.Although FAR genes are involved in the biosynthesis of sex pheromones in moths,the key FAR gene of Spodoptera litura remains unclear.In this work,we predicted 30 FAR genes from the S.litura genome and identified a domain duplication within gene SlitFAR3,which exhibited high and preferential expression in the sexually mature female pheromone glands(PGs)and a rhythmic expression pattern during the scotophase of sex pheromone production.The molecular docking of SlitFAR3,as predicted using a 3D model,revealed a co-factor NADPH binding cavity and 2 substrate binding cavities.Functional expression in yeast cells combined with comprehensive gas chromatography indicated that the SlitFAR3 gene could produce fatty alcohol products.This study is the first to focus on the special phenomenon of FAR domain duplication,which will advance our understanding of biosynthesis-related genes from the perspective of evolutionary biology. | Biyun Zhang Fengqi Li Cheng Qu Hongxia Duan Yuejun Fu Chen Luo | 2023 | Insect Science2023，30，3： | 0 |
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