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| 1 | Expression of Acyl-lipid 12-desaturase Gene in Prokaryotic and Eukaryotic Cells and Its Effect on Cold Stress Tolerance of Potato显示文摘We report the expression profile of acyl-lipid 12-desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coli) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants was observed for lines 2 (73%) and 3 (41%). Finally, our results showed that desaturase could enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product was higher than in the case of reporter lichenase gene absence in the construction. | Reza Maali Amiri Natalia O. Yur'eva Khristina R. Shimshilashvili Irina V. Goldenkova-Pavlova Vasiliy P. Pchelkin Elmira I. Kuznitsova Vladimir D. Tsydendambaev Tamara I. Trunova Dmitry A. Los Gholamreza Salehi Jouzani Alexander M. Nosov | 2010 | Journal of Integrative Plant Biology2010，52，3： | 6 |
| 2 | Finemappingandconservationa-nalysisoflinearB-cellepitopesofpestedespetitsruminantsvirusnucleoprotein显示文摘 | YuR FanX XuW etal | 2015 | VetMicrobiol2015，175，1： | 1 |
| 3 | Gas-filled metal-ceramic high-pressure spark-gap switches显示文摘 | Avilov E A Yur' ev A L | 2000 | Instruments and Experimental Techniques2000，43，2： | 1 |
| 4 | Diffuse pollution loading from urban stormwater runoff in Daejeon city, Korea显示文摘 | Geonha Kim Joonghyun Yur Jeongkon Kim | 2006 | Journal of Environmental Management2006，，1： | 1 |
| 5 | High-current electron gun with a field-emission cathode and diamond grid显示文摘 | N. N. Dzbanovskii P. V. Minakov A. A. Pilevskii A. T. Rakhimov B. V. Seleznev N. V. Suetin A. Yu. Yur’ev | 2005 | Technical Physics2005，，10： | 1 |
| 6 | Delayed 'Choice' Quantum Eraser显示文摘 | KIM YH YUR SCULLY M O | 2000 | Phys Rev Lett2000，84，： | 1 |
| 7 | Lipid fatty acid composition of potato plants transformed with the D12 - desaturase gene from cyanobacterium 显示文摘 | MAALI -AMIRI R GOLDENKOVA- PAVLOVA I V YUR' EVA N O | 2007 | Russian Journal of Plant Physiology2007，54，5： | 1 |
| 8 | Antioxidant vitamin and mineral levels in sheep with fluorosis显示文摘 | Yasar S F Yur | 2008 | Biol Trace Elem Res2008，123，13： | 1 |
| 9 | 264/AVC 显示文摘 | Xu Xiaozhong He Yur Improvements on {ast motion estimation strategy for H | 2008 | IEEE Transactions on Circuits and Systems forVideoTechnology2008，18，3： | 1 |
| 10 | Pimitary tunor transforming gene(PTTG) regulates placental JEG-3 cell division and survival:evidence from live cell imaging显示文摘 | YuR RenSG HorwitzGA etal | 2000 | Mol Endocrinol2000，14，8： | 1 |
| 11 | Diffuse pollution loading from urban stormwater runoffin Daejeon City,Korea显示文摘 | Kim G H Yur J H Kim J K | | 0，，01： | 1 |
| 12 | Organicvegetableproductiontechnologyintegrationresearchanddemonstration显示文摘 | JiaY-H(贾永华) YuR(于蓉) FengZ-H(冯志红) etal | 2014 | 中国科技成果2014，，22： | 1 |
| 13 | Effect of lycopene application in rats with experimental diabetes using lipoprotein, paraoxonase and cy- tokines显示文摘 | Yeqin SC Yur F Ceylan E | 2013 | J Memhr Biol2013，246，8： | 1 |
| 14 | Environmental effects on the water absorption and mechanical properties of carbon fibre reinforced PPS and PEEK composites显示文摘 | Ma C Yur S W | 1991 | Polymer Engineering and Science1991，31，1： | 1 |
| 15 | Regularizability of linear time-invariant descriptor systems under decentralized control显示文摘 | YUR | 2005 | Automatica2005，41，9： | 1 |
| 16 | Effect of lycopene application in rats with experimental diabetes using lipoprotein,paraoxonase and cytokines显示文摘 | Yegin SC Yur F Ceylan E | 2013 | J Membr Biol2013，246，8： | 1 |
| 17 | Diffuse pollution loading from urban stormwater runoff in Daejeon city, Korea 显示文摘 | Kim G Yur J Kim J | 2006 | Journal of Environment Management2006，7，： | 1 |
| 18 | Application of fiber Bragg grating level sensor and Fabry-Pérot pressure sensor to simultaneous measurement of liquid level and specific gravity显示文摘 | Lai C W Lo Y L Yur J P | 2012 | IEEE Sensors Journal2012，12，4： | 1 |
| 19 | Strength and Behavior of Grouted pile-sleeve Connections 显示文摘 | Lamport W B Jirsa J O Yur a J O | 1991 | Journal of Structural of Engineering1991，117，8： | 1 |
| 20 | Diffuse pollution loading from urban stormwater runoff in Daejeon city,Korea显示文摘 | Kima G H Yur J Y Kim J K | 2007 | Journal of Environmental Management2007，85，： | 1 |
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