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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | A novel protein-repellent dental composite containing 2-methacryloyloxyethyl phosphorylcholine显示文摘Secondary caries due to biofilm acids is a primary cause of dental composite restoration failure.To date,there have been no reports of dental composites that can repel protein adsorption and inhibit bacteria attachment.The objectives of this study were to develop a protein-repellent dental composite by incorporating 2-methacryloyloxyethyl phosphorylcholine(MPC) and to investigate for the first time the effects of MPC mass fraction on protein adsorption,bacteria attachment,biofilm growth,and mechanical properties.Composites were synthesized with 0(control),0.75%,1.5%,2.25%,3%,4.5%and 6%of MPC by mass.A commercial composite was also tested as a control.Mechanical properties were measured in three-point flexure.Protein adsorption onto the composite was determined by the microbicinchoninic acid method.A human saliva microcosm biofilm model was used.Early attachment at 4 h,biofilm at 2 days,live/dead staining and colony-forming units(CFUs) of biofilms grown on the composites were investigated.Composites with MPC of up to 3%had mechanical properties similar to those without MPC and those of the commercial control,whereas 4.5%and 6%MPC decreased the mechanical properties(P<0.05).Increasing MPC from 0 to 3%reduced the protein adsorption on composites(P<0.05).The composite with 3%MPC had protein adsorption that was 1/12 that of the control(P<0.05).Oral bacteria early attachment and biofilm growth were also greatly reduced on the composite with 3%MPC,compared to the control(P<0.05).In conclusion,incorporation of MPC into composites at 3%greatly reduced protein adsorption,bacteria attachment and biofilm CFUs,without compromising mechanical properties.Protein-repellent composites could help to repel bacteria attachment and plaque build-up to reduce secondary caries.The protein-repellent method might be applicable to other dental materials. | Ning Zhang Chen Chen Mary AS Melo Yu-Xing Bai Lei Cheng Hockin HK Xu | 2015 | International Journal of Oral Science2015，7，2： | 8 |
| 3 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 4 | Influence of IFNα-2b and BCG on the release of TNF and IL-1 by Kupffer cells in rats with hepatoma显示文摘INTRODUCTIONKupffer cells are residential macrophages in the liver ,which play a critical role in the maintenance of normal liver function and in immunal surveilance of hepatocellular carcinoma (HCC) and other cancers[1].The biological immune modulants have cancers[2].In our previous studies ,the combined use of biological immune modulants showed better dffects . | Xian Yong Bai~1 Xiu Hong Jia~2 Ling Zhong Cheng~3 Yun Di Gu~3 ~1Department of Histology and Embryology,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~2Departrnent of Pediatrics,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~3Department of Histology and Embryology,Shanghai Medical University,Shanghai 200032,ChinaDr.Xian Yong Bai graduated from the Shanghai Medical University as a postgraduate in 1995,Professor of Histology and Embryology,specialized in hepatic tumor immunology,having 36 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 7 |
| 5 | Traumatic brain inju- ry-induced cognitive and histological deficits are attenuated by delayed and chronic treatment with the 5-HT(1A)-receptor ago- nist buspirone 显示文摘 | Olsen AS Sozda CN Cheng JP | 2012 | J Neurotrauma2012，29，10： | 1 |
| 6 | Expression of HBx and COX-2 in chronic hepatitis B,cirrhosis and hepatocellular carcinoma:implication of HBx in upregulation of COX-2显示文摘 | Cheng AS Chan HL Leung WK | 2004 | Mod Pathol2004，17，10： | 1 |
| 7 | Cyclooxygenase-2 pathway correlates with vascular endothelial growth factor expression and tumor anglogenesis in hepatitis B virus associated hepatocytes carcinoma 显示文摘 | Cheng AS Chan HL TO KF | 2004 | Int J Oncol2004，24，4： | 1 |
| 8 | High serum interlenkin-6 level predicts future hepatocellular carcinoma development in patients with chronic hepatitis B显示文摘 | Wong VW Yu J Cheng AS | 2009 | Int J Cancer2009，124，12： | 1 |
| 9 | High serum interleukin-6 level predicts future hepatocellular carcinoma development in patients with chronic hepatitis B显示文摘 | Wong VW Yu J Cheng AS | 2009 | Int J Cancer2009，124，12： | 1 |
| 10 | Matrix metallopro-teinase 2 and basement membrane in-tegrity: a unifying mechanism for progressive renal injury 显示文摘 | Cheng S Polio AS Mabimbar R | 2006 | FASEB J2006，20，11： | 1 |
| 11 | Intravitreal sustained release corticosteroid-5-fluorurail conjugate in the treatment of experimental proliferative vitreoretinopathy显示文摘 | Berger AS Cheng CK Pearson PA Ashton P Crooks PA Cynkowski T | 1996 | Invest Ophthalmol Vis Sci1996，37，： | 1 |
| 12 | High serum interleukin-6level predicts future hepatocellular carcinoma development inpatients with chronic hepatitis B显示文摘 | Wong VW Yu J Cheng AS | 2009 | Int J Cancer2009，124，12： | 1 |
| 13 | Cyclooxygenase-2 pathway correlates with vascular endothelial growth factor expression and tumor angiogenesis in hepatic B virus-associated hepatocelluar carcinoma显示文摘 | Cheng AS Chan HL To KF | 2004 | In J Oncol2004，24，4： | 1 |
| 14 | Trop2 i- dentifies a subpopulation ofmurine and human prostate basalcells with stem cell characteristics显示文摘 | GOLDSTEIN AS LAWSON DA CHENG D | 2008 | Proc Natl Acad Sci LISA2008，105，20： | 1 |
| 15 | Cell cycle-related kinase is a direct androgen receptor-regulated gene that drives β-cateninfr cell factor-dependent hepatocarcino- genesis 显示文摘 | Feng H Cheng AS Tsang DP | 2011 | J Clin Invest2011，121，8： | 1 |
| 16 | Xingpi Yang Er particles combination in the treatment of infantile pneumonia diarrhea in 90cases of clinical observation显示文摘 | Zhou Gaoliang Cheng FangHai Huang Xianlai such as | 2012 | Chin Trad Chin Med Sci Tech2012，19，1： | 1 |
| 17 | Bcl -xL is an antiapoptotic regulator for postnatal CNS neurons显示文摘 | Parsadanian AS Cheng Y Keller- Peck CR | 1998 | J Neurosci1998，18，3： | 1 |
| 18 | FK-16derived from the anticancer peptide LL-37induces caspase-independent apoptosis and autophagic cell death in colon cancer cells显示文摘 | Ren SX Shen J Cheng AS | 2013 | PLoS One2013，8，63： | 1 |
| 19 | Presenilin-mediated modulation of eapacitative calcium entry 显示文摘 | Yoo AS Cheng I Chung S | 2000 | Neuron2000，27，3： | 1 |
| 20 | TNF inhibits Notch-1 in skeletal muscle cells by Ezh2 and DNA methylation mediated repression :implieations in duchenne muscular dystrophy 显示文摘 | Acharyya S Sharma SM Cheng AS | 2010 | PLoS One2010，5，12： | 1 |
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