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| 1 | Effect of acetazolamide on stable carbon isotope fractionation in Chlamydomonas reinhardtii and Chlorella vulgaris显示文摘The effect of extracellular carbonic anhydrase (CAex) on stable carbon isotope fractionation in algae is still unclear. The stable carbon isotope composition and algal growth in the presence and absence of the membrane-impermeable CA inhibitor acetazolamide were compared in Chlamydomonas reinhardtii and Chlorella vulgaris. The CAex of both algal species contributed about 9‰ of the stable carbon isotope fractionation and exhibited a dosage effect. Therefore, evidence in vivo that CAex leads to a larger carbon isotope fractionation of algae is presented. | WU YanYou XU Ying LI HaiTao XING DeKe | 2012 | Chinese Science Bulletin2012，57，7： | 10 |
| 2 | Quantification of photosynthetic inorganic carbon utilisation via a bidirectional stable carbon isotope tracer显示文摘The amount of bicarbonate utilised by plants is usually ignored because of limited measurement methods.Accordingly, this study quantified the photosynthetic assimilation of inorganic carbon(CO_2and HCO_3^-) by plants. The net photosynthetic CO_2assimilation(PN), the photosynthetic assimilation of CO_2 and bicarbonate(PN'),the proportion of increased leaf area(fLA) and the stable carbon isotope composition(δ^(13)C) of Orychophragmus violaceus(Ov) and Brassica juncea(Bj)under three bicarbonate levels(5, 10 and 15 mm NaHCO_3)were examined to determine the relationship among PN,PN' and fLA. PN', not PN,changed synchronously with fLA.Moreover, the proportions of exogenous bicarbonate and total bicarbonate(including exogenous bicarbonate and dissolved CO_2-generated bicarbonate) utilised by Ov were 2.27 % and 5.28 % at 5 mm bicarbonate, 7.06 % and13.28 % at 10 mm bicarbonate, and 8.55 % and 17.31 % at15 mm bicarbonate, respectively. Meanwhile, the proportions of exogenous bicarbonate and total bicarbonate utilised by Bj were 1.77 % and 3.28 % at 5 mm bicarbonate,2.11 % and 3.10 % at 10 mm bicarbonate, and 2.36 % and3.09 % at 15 mm bicarbonate, respectively. Therefore, the dissolved CO_2-generated bicarbonate and exogenous bicarbonate are important sources of inorganic carbon for plants. | Hongtao Hang Yanyou WU | 2016 | Acta Geochimica2016，35，2： | 6 |
| 3 | Empowering fall webworm surveillance with mobile phone-based community monitoring: a case study in northern China显示文摘Recent advances in information and communication technologies, such as mobile Internet and smartphones, have created new paradigms for participatory environment monitoring. The ubiquitous mobile phones with capabilities such as a global positioning system,camera, and network access, offer opportunities to establish distributed monitoring networks that can perform a wide range of measurements for a landscape. This study examined the potential of mobile phone-based community monitoring of fall webworm(Hyphantria cunea Drury).We built a prototype of a participatory fall webworm monitoring system based on mobile devices that streamlined data collection, transmission, and visualization. We also assessed the accuracy and reliability of the data collected by the local community. The system performance was evaluated at the Ziya commune of Tianjin municipality in northern China, where fall webworm infestation has occurred. The local community provided data with accuracy comparable to expert measurements(Willmott's index of agreement>0.85). Measurements by the local community effectively complemented remote sensing images in both temporal and spatial resolution. | Chengbo Wang Yanyou Qiao Honggan Wu Yuanfei Chang Muyao Shi | 2016 | Journal of Forestry Research2016，27，6： | 4 |
| 4 | Effects of carbon anhydrase on utilization of bicarbonate in microalgae:a case study in Lake Hongfeng显示文摘A bidirectional labeling method was established to distinguish the proportions of HCO_3^- and CO_2 utilization pathways of microalgae in Lake Hongfeng. The method was based on microalgae cultured in a medium by adding equal concentrations of NaH^(13)CO_3 with different δ^(13)C values simultaneously. The inorganic carbon sources were quantified according to the stable carbon isotope composition in the treated microalgae. The effects of extracellular carbonic anhydrase(CAex) on the HCO^-_3 and CO_2 utilization pathways were distinguished using acetazolamide, a potent membrane-impermeable carbonic anhydrase inhibitor. The results show utilization of the added HCO^-_3 was only 8% of the total carbon sources in karst lake. The proportion of the HCO^-_3 utilization pathway was 52% of total inorganic carbon assimilation. Therefore, in the natural water of the karst area, the microalgae used less bicarbonate that preexisted in the aqueous medium than CO_2 derived from the atmosphere. CAex increased the utilization of inorganic carbon from the atmosphere. The microalgae with CAex had greater carbon sequestration capacity in this karst area. | Haitao Li Yanyou Wu Lihua Zhao | 2018 | Acta Geochimica2018，37，4： | 4 |
| 5 | Effect of Zn deficiency and excessive bicarbonate on the allocation and exudation of organic acids in two Moraceae plants显示文摘The effect of zinc(Zn) deficiency and excessive bicarbonate on the allocation and exudation of organic acids in plant organs(root, stem, and leaf) and root exudates of two Moraceae plants(Broussonetia papyrifera and Morus alba) were investigated. Two Moraceae plants were hydroponically grown and cultured in nutrient solution in four different treatments with 0.02 mM Zn or no Zn,combined with no or 10 mM bicarbonate. The variations of organic acids in different plant organs were similar to those of root exudates in the four treatments except B. papyrifera, which was in a treatment that was a combination of 0.02 mM Zn and no bicarbonate. The response characteristics in the production, translocation, and allocation of organic acids in the plant organs and root exudates varied with species and treatments. Organic acids in plant organs and root exudates increased under Zn-deficient conditions,excessive bicarbonate, or both. An increase of organic acids in the leaves resulted in an increase of root-exuded organic acids. B. papyrifera translocated more oxalate and citrate from the roots to the rhizosphere than M. alba under the dual influence of 10 mM bicarbonate and Zn deficiency. Organic acids of leaves may be derived from dark respiration and photorespiration. By comparison, organic acids in stems, roots, and root exudates may be derived from dark respiration and organic acid translocation from the leaves. These results provide evidence for the selective adaptation of plants to environments with low Zn levels or high bicarbonate levels such as a karst ecosystem. | Kuan Zhao Yanyou Wu | 2018 | Acta Geochimica2018，37，1： | 2 |
| 6 | Does bicarbonate affect the nitrate utilization and photosynthesis of Orychophragmus violaceus?显示文摘The effect of bicarbonate(HCO_3^-) on the growth and development of plants varies by species. To better understand inorganic carbon and nitrogen assimilation changes of karst-adaptable plants under different HCO_3^- treatments, we conducted experiments on seedlings and in vitro plantlets of Orychophragmus violaceus(Ov).We found that the vital photosynthesis potential(as measured by net photosynthetic rate, actual photochemical efficiency of photosystem-II, photochemical quenching coefficient, and the instantaneous carbon isotope ratio of3-phosphoglycerate) was consistent under different HCO_3^- treatments of Ov. Bicarbonate's lack of effect on carbon assimilation of Ov may be related to carbonic anhydrase in Ov converting HCO_3^- to H2 O and CO2. In this way, Ov could prevent HCO_3^- ion toxicity and high pH from harming its growth and development under HCO_3^- stress.This study also found that high HCO_3^- concentrations could promote nitrogen assimilation and utilization of Ov through changes in related indexes(foliar nitrogen isotope fractionation ratio, stable nitrogen isotope assimilation ratio, foliar stable nitrogen isotope fractionation, nitrate nitrogen utilization efficiency, and nitrate utilization share)under different HCO_3^- treatments. Bicarbonate has different effects on photosynthesis and on inorganic nitrogen assimilation of Ov, which may be connected tophotosynthesis providing electrons for nitrate/nitrite reduction through the photosynthetic chain. | Ye Lu Yanyou Wu Kaiyan Zhang | 2018 | Acta Geochimica2018，37，6： | 2 |
| 7 | Bicarbonate use and carbon dioxide concentrating mechanisms in photosynthetic organisms显示文摘Photosynthesis is crucial to the reduction of carbon dioxide in the atmosphere.The key enzyme of photosynthesis,Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco),has two mutably competing substrates,CO2 and O2.It has features of carboxylase and oxygenase.Rubisco performs the function of carboxylase to reduce inorganic carbon to form organic substances,which precondition is that more carbon dioxide accumulates around it.Carbon dioxide concentrating mechanisms(CCMs)are vital to cope with the limit of carbon dioxide.Various bicarbonate use pathway has a differential contribution to inorganic carbon assimilation.Bicarbonate transport,extracellular bicarbonate dehydration,or H+-ATPase-driven bicarbonate uptake,which induced CCMs,can support a considerable share of photosynthesis in photosynthetic organisms.However,CCMs in thylakoid membranes may be the most important.The CCMs occurred in the plasma membrane were secondary,evolutionary,and inducible,while CCMs coupled with photosynthetic oxygen evolution in thylakoid membranes,were primitive,major,and indispensable.A hypothetical schematic model of CCMs occurred in the plasma membrane and thylakoid membranes being proposed. | Yanyou Wu | 2021 | Acta Geochimica2021，40，5： | 2 |
| 8 | Photosynthetic capability and Fe, Mn, Cu, and Zn contents in two Moraceae species under different phosphorus levels显示文摘The strong adaptability of Broussonetia papyrifera(L.) Vent. to low phosphorus(P) conditions can be attributed to the large amount of root-exuded organic acids and the high ef?ciency of P extraction. However,microelement contents are in?uenced by low-P stress, and their effects on the photosynthetic capability of B. papyrifera remain unknown. In this study, we investigated the effects of low-P treatment on net photosynthetic rate(P_N);chlorophyll a ?uorescence(ChlF) characteristics; and Fe,Mn, Cu, and Zn contents of B. papyrifera and Morus alba L. seedlings. Results show that B. papyrifera exhibited better photosynthetic capability under moderate P de?-ciency(0.125, 0.063, and 0.031 mmol/L P treatments),whereas the photosynthetic capability of M. alba decreased under moderate and severe P de?ciency(0.016 and0 mmol/L P treatments). Under moderate P de?ciency, the decrease in Cu and Zn contents in B. papyrifera was lower than that in M. alba. Under severe P de?ciency, a considerable decrease of photosynthetic capability in B. papyrifera and M. alba was associated with low Cu and Zn contents. The P N of the two Moraceae species exhibited a better correlation with Cu and Zn contents than with Fe or Mn content. P de?ciency could not only decrease cyclic photophorylation and photosynthetic ef?ciency, but could also affect the stability of thylakoid membrane structure and electron transport ef?ciency by in?uencing the contents of Cu or Zn, thereby affecting photosynthesis. | Deke Xing Yanyou Wu Rui Yu Yansheng Wu Chuan Zhang Zheng Liang | 2016 | Acta Geochimica2016，35，3： | 2 |
| 9 | The influence of three mangrove species on the distribution of inorganic nitrogen and phosphorus in the Quanzhou Bay estuarine wetland soils显示文摘This study aims to investigate the effects of region and three regional dominated mangrove species(Avicennia marina, Aegiceras corniculatum and Kandelia candel) on the distribution of inorganic nitrogen and phosphorus. Measurement of the inorganic nitrogen and phosphorus and enzymatic activities was carried out in soils covered by three mangrove species in the Quanzhou Bay estuarine wetlands, a typical coastal wetland in China.Species with a higher biomass in upstream and midstream absorb more nitrogen from soils, and the retention of the available phosphorus in the soils of different regions causes the regional variation of phosphorus. In areas dominated by A. marina, nitrate nitrogen is lower while available phosphorus is higher. Meanwhile, nitrate nitrogen and available phosphorus are higher in the soils covered by K. candel.Moreover, all three species affect the elemental and enzymic stoichiometry. The mangrove species influences the diversity of the elemental and enzymic stoichiometric relationship through differential microenvironments, which induce the biodiversity of wetland ecosystems. Thus, this study may facilitate a better understanding of the transformation ability of mangroves to nitrogen and phosphorus and will therefore be beneficial for providing a basis for the ecological restoration of estuarine wetlands. | Guiyao Zhou Yanyou Wu Deke Xing Mingming Zhang Rui Yu Weiyi Qiao Qaiser Javed | 2016 | Acta Geochimica2016，35，1： | 1 |
| 10 | Study on photosynthetic characteristics of Orychophragmus violaceus related to shade-tolerance显示文摘 | Yanyou Wu Pingping Li Yuguo Zhao Jizhan Wang Xiangming Wu | 2007 | Scientia Horticulturae2007，，2： | 1 |
| 11 | Comparison of carbonic anhydrase activity among various species of plantlets显示文摘 | Wu Yanyou Li Xiteng Li Pingping | 2006 | Plant Cell Tissue and Organ Culture2006，84，1： | 1 |
| 12 | Combined effect of bicarbonate and water in photosynthetic oxygen evolution and carbon neutrality显示文摘Carbon neutrality is widely concerned and highly valued by many countries.Biosphere has always maintained the balance between oxidized organic substances and assimilated organic matter,resulting in netzero carbon dioxide(CO_(2)) emissions and maintaining its own carbon neutrality.Nature has set a good example for human beings to coordinate oxygen(O_(2)) balance and CO_(2)balance,and achieve carbon neutrality.How does photosynthetic oxygen evolution initiate carbon and water neutrality?My synthesis shows that photo system Ⅱ functions as carbonic anhydrase to catalyze the reaction of CO_(2)hydration under physiological conditions,and CO_(2)hydration coupled with chemical equilibrium,H^(+)+HCO_(3)^(-)→1/2O_(2)+2e^(-)+2H^(+)+CO_(2),occurs in a photosystem Ⅱ corecomplex.Meanwhile,I focused on the revisiting of four classical heavy oxygen(O^(18)) labeling experiments and found that bicarbonate can promote photo synthetic oxygen evolution,and that photo synthetic oxygen evolution can alternately come from bicarbonate and water,not only water.Bicarbonate photolysis and water photolysis account for half of the photo synthetic oxygen evolution respectively,which can well explain the bicarbonate effect,Dole effect and plants’ environmental adaptability.Photosynthetic oxygen evolution initiated the journey of water metabolism and carbon metabolism in nature,which led to the coupling as 1:1(mol/mol) stoichiometric relationship between the reduction of CO_(2)and oxidation of organic carbon,coordinated the evolution of the atmosphere,hydrosphere,lithosphere and biosphere,and realized 'carbon neutrality' in the whole Earth system. | Yanyou Wu | 2023 | Acta Geochimica2023，42，1： | 1 |
| 13 | The δ^15N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations显示文摘Natural nitrogen isotope composition(δ^(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. δ^(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar δ^(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus(Ov) and Brassica napus(Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar δ^(15)N and nitrate assimilation.The plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication stage.Nitrogen isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase activity.Moreover, high external nitrate concentrations couldcontribute to improved foliar nitrogen content and enhanced nitrate assimilation ability. | Kaiyan Zhang Yanyou Wu | 2017 | Acta Geochimica2017，36，2： | 1 |
| 14 | Comparison of carbonic anhydrase activity among various species of planttets显示文摘 | Wu Yanyou Li Xiteng Li Pingping | 2005 | Plant Cell Tissue and Organ Culture2005，84，1： | 1 |
| 15 | The distribution characteristics of nitrogen and phosphorus in the ecological system of Mt. Beigu wetland显示文摘The Mt. Beigu wetland, which has undergone periodical changes in water level, lies by the Yangtze River, and its dominant plants are Phragmites communis, Phalaris arundinacea and Polygonum lapathifolium. In order to study the distribution characteristics of nitrogen and phosphorus in the ecological system of the Mt. Beigu wetland, the authors measured the contents of total nitrogen and total phosphorus in Phragmites communis, Phalaris arundinacea and Polygonum lapathifolium, and the contents of nitrogen and phosphorus in the wetland soils with different plant species. In addition the authors investigated the influence of various plants on the spatial and seasonal (spring/autumn) distributions of nitrogen and phosphorus in the wetland soil. The contents of nitrogen and phosphorus in Phalaris arundinacea are significantly higher than those of the other two plant species in the same part. Secondary pollution of phosphorus in the wetland results mainly from Phalaris arundinacea. Phragmites communis effectively carries away nitrogen and phosphorus in the wetland soil in the wet season. The capacity of Polygonum lapathifolium to remove nitrogen is lowest among the 3 species of plants. These findings offer a theoretical foundation for the selection of plant species to restore the ecological environment and for the selection of time and depth for purging silt on the riverside wetland. | WU Yanyou | 2009 | Chinese Journal Of Geochemistry2009，28，1： | 1 |
| 16 | Spatial and seasonal variation of salt ions under the influence of halophytes, in a coastal flat in eastern China显示文摘 | Yanyou Wu Rongcheng Liu Yuguo Zhao Pingping Li Congqiang Liu | 2009 | Environmental Geology2009，，7： | 1 |
| 17 | Effects of low nutrition on photosynthetic capacity and accumulation of total N and P in three climber plant species显示文摘To study the effects of low nutrition on pho tosynthetic capacity and accumulation of total nitrogen(N) and phosphorus(P) in three climber plant species Pharbitis nil(Linn.) Choisy, Lonicera japonica Thunb. and Parthenocissus tricuspidata(Sieb.et Zucc.) Planch, al climber plants were exposed to low nutrition at 6 levels(Hoagland solution as control, 1/2, 1/4, 1/8, 1/16 and 1/32 strength Hoagland solution) for 30 days. Photosynthetic capacity was determined by measuring leaf chlorophyl fluorescence, chlorophyll content, carbonic anhydrases activity and growth. Accumulation of total N and P was studied by measuring N and P content in plant tissues. Low nutrition decreased the photosynthetic capacity of P. nil while L. japonica maintained high photosynthetic capacity under low nutrition. Photosynthetic apparatus of P. tricu spidata suffered no damage when exposed to low nutrition L. japonica and P. tricuspidata had better adaptability to low nutrition than P. nil. With a faster growth rate, P. ni consumed more nutrition(N and P), and its growth was mainly affected by P deficiency under low nutrition Although L. japonica suffered damage from N and P deficiency simultaneously, but the nutrient deficiency was not serious except for 1/32-strength Hoagland solution P. tricuspidata grew slowly, so its requirement of N and Pwere the least, even if it was mainly affected by the P deficiency, it could still grow well under low nutrition.With the consideration of fertilizing N and P fertilizers in karst areas which were with lower N and P contents, plant species, N/P ratio threshold and low nutrition level should be taken into account synchronously. This study could provide a general consideration for the planning and developing low nutrition resistant plants and fertilizing the three climber plant species in the low nutrition environment. | Deke Xing Yanyou Wu | 2015 | Chinese Journal Of Geochemistry2015，34，1： | 1 |
| 18 | Is bicarbonate directly used as substrate to participate in photosynthetic oxygen evolution显示文摘If the photosynthetic organisms assimilated only CO_(2) in the Archean atmosphere,hydroxide ion in the Archean seawater would not increase.If plants would not consume bicarbonate as a direct substrate during photosynthesis,it is difficult to explain the evolution of Earth's environment.To date,it is generally accepted that photosynthetic O_(2) evolution of plants come from water photolysis.However,it should be debated by evaluating the effect of bicarbonate in photosynthetic O_(2) evolution,analyzing the role of carbonic anhydrase(CA) in photosynthetic O_(2) evolution,and the relationship between thylakoid CA and photosynthetic O_(2) evolution.In the paper,I propose that bicarbonate is directly used as substrate to participate in photosynthetic O_(2) evolution.The rationality of bicarbonate photolysis of plants is discussed from the thermodynamics and evolution of Earth's environment.The isotopic evidence that bicarbonate is not the direct substrate of photosynthetic O_(2) release is reexamined,and the new explanation of bicarbonate photolysis in photosynthetic O_(2) evolution is proposed. | Yanyou Wu | 2021 | Acta Geochimica2021，40，4： | 1 |
| 19 | Composition and activity of external carbonic anhydrase of microalgae from karst lakes in China显示文摘 | WU Yanyou LI Pingping WANG Baoli | 2008 | Phycological Research2008，56，2： | 1 |
| 20 | Biosorption of trace metals from aqueous multimetal solutions by green microalgae显示文摘Two strains of green microalgae C.reinhardti and C.pyrenoidosa were examined for their biosorption of Mn2+,Fe2+,Co2+,Ni2+,Cu2+,Zn2+,and Cd2+from aqueous multi-metal solutions.A wide range of biosorption capacities can be observed due to different strains of microalgae and different species of trace metals.This characteristic was ascribed to the distinct components and structures of algal cell walls and the different physicochemical properties of trace metals,such as atomic weight and ion density.C.pyrenoidosa showed higher uptake capacities than C.reinhardti and both of them had a preference for the uptake of cadmium over others in the trace metal solution,suggesting they can be a good biomaterial for biosorption of cadmium.Live microalgal cells displayed a more complex sorption process than dead microalgal cells because of cell assimilation. | ZHAO Yanchuang WANG Baoli LIU Congqiang WU Yanyou | 2013 | Chinese Journal Of Geochemistry2013，32，4： | 0 |
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