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| 1 | Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems显示文摘The CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we developed Hi-TOM(available at http://gffzz103f3ce5d9b14419hvbqcukqw6b6k6xc9.ffgz.tsg.suse.edu.cn/hi-tom/), an online tool to track the mutations with precise percentage for multiple samples and multiple target sites. We also described a corresponding next-generation sequencing(NGS) library construction strategy by fixing the bridge sequences and barcoding primers. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations frequently induced by genome editing. Hi-TOM does not require special design of barcode primers,cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems. | Qing Liu Chun Wang Xiaozhen Jiao Huawei Zhang Lili Song Yanxin Li Caixia Gao Kejian Wang | 2019 | Science China(Life Sciences)2019，62，1： | 37 |
| 2 | Growth,invasion,metastasis,differentiation,angiogenesis and apoptosis of gastric cancer regulated by expression of PTEN encoding products显示文摘AIM: To investigate expression of PTEN in gastric cancer and to explore its roles in tumorigenesis and progression of gastric cancer.METHODS: Formalin-fixed and paraffin-embedded tissues of adjacent non-tumor mucosa and primary foci from 113cases of gastric cancers were studied for the expression of PTEN and Caspase-3 andmicrovessel density (MVD)by streptavidin-peroxidase (S-P) immunohistochemistry with antibodies against PTEN, Caspase-3, and CD34. The relationship between PTEN and Caspase 3 expression and clinicopathological parameters of tumors was compared.RESULTS: Primary gastric cancer cells expressed PTEN less frequently than adjacent epithelial cells of primary foci (54.9% vs89.4%; P=0.000, χ2=33.474). PTEN expression was significantly associated with invasive depth (P=0.003,rs=0.274), metastasis (P=0.036, rs=0.197), growth pattern (P=0.008, rs=0.282), Lauren′s classification (P=0.000,rs=0.345), and histological classification (P=0.005, rs=0.262)of tumors, but not with tumor size (P=0.639, rs=0.045),Borrmann′s classification (P=0.544, rs=0.070) or TNM staging (P=0.172, rs=0.129). PTEN expression was negatively correlated with MDV in primary gastric cancer (P=0.020,F=5.558). Primary gastric cancer cells showed less frequent immunoreactivity to Caspase-3 than adjacent epithelial cells of primary foci (32.7 % vs 50.4 %; P=0.007,χ2=7.286).Caspase-3 expression was dependent of PTEN expression in primary gastric cancer cells (P=0.000, χ2=15.266).CONCLUSION: Down-regulated expression of PTEN plays an important role in tumorigenesis, progression, growth,differentiation and angiogenesis of gastric cancer. Low expression of PTEN can decrease expression of Caspase-3to disorder apoptosis of tumor cells, which might explain the molecular mechanisms of PTEN contributions to tumorigenesis and progression of gastric cancer. | Wei-GuoJiang Yin-ChangZhang YanXin Hua-ChuanZheng Yi-LingLi Jin-MinSun Xue-FeiYang Xiao-HanLi | 2003 | World Journal of Gastroenterology2003，9，8： | 33 |
| 3 | Pathobiological behavior and molecular mechanism of signet ring cell carcinoma and mucinous adenocarcinoma of the stomach:A comparative study显示文摘AIM:To elucidate the distinctive pathobiological behavior between signet ring cell carcinoma (SRC) and mucinous adenocarcinoma of the stomach.METHODS: Based on the histological growth patterns and cell-functional differentiation classifications of stomach carcinoma, we conducted a series of comparative studies.All paraffin-embedded and frozen blocks were collected from the files of Cancer Institute of China Medical University. On the basis of histopathological observation, we applied enzymatic and mucous histochemistry, immunohistochemistry,flow cytometry (FCM) and molecular biology to compare these two categories of gastric cancers in terms of the DNA ploidy, proliferative kinetics, the expression of gastric carcinoma associated gene product and instabilities of mitochondrial DNA (mtDNA).RESULTS:Gastric SRC was commonly seen in females below 45 years, mostly presenting diffuse growth and ovary or uterine cervix metastasis. The majority of SRC were absorptive and mucus-producing functional differentiation type (AMPFDT), which growth relied on estrogen. Meanwhile,stomach mucinous adenocarcinomas were mostly observed in males over 50 years, prone to massive growth or nest growth and extensive peritoneal infiltration, showing two categories of cell-functional differentiation types: AMPFDT and mucus-secreting functional differentiation type (MSFDT).Expressions of ER, enzyme c-PDE and 67kDaLN-R in SRC were evidently higher than that in mucinous adenocarcinoma,while expressions of LN, CN-Ⅳ, CD44v6, and PTEN protein were obviously lower in SRC than that in mucinous adenocarcinoma (P<0.05).There was no statistic significance in VEGF, ECD and instabilities of mtDNA (P>0.05) between the above two gastric carcinomas.CONCLUSION: Though SRC and mucinous adenocarcinoma were both characterized by abundant mucus-secretion, they were quite different in morphology, ultrastructure, cell-functional differentiation and protein expression, indicating different mechanisms of carcinogenesis. We concluded that combining histological growth patterns, cell-functional differentiation type with tumor related markers might be significant in early diagnosis and prognosis assessment for SRC and mucinous adenocarcinoma of the stomach. | Xue-FeiYang LinYang Xiao-YunMao Dong-YingWu Su-MinZhang YanXin | 2004 | World Journal of Gastroenterology2004，10，5： | 33 |
| 4 | EXPRESSION OF MASPIN AND KAI1 AND THEIR CLINICOPATHOLOGICAL SIGNIFICANCE IN CARCINOGENESIS AND PROGRESSION OF GASTRIC CANCER显示文摘To investigate the roles of maspin and kai1 expression in tumorigenesis and progression of gastric cancer. Methods Maspin and kai1 expressions were detected in normal gastric mucosa (n = 182), gastric dysplasia (n = 69), and gastric cancer (n = 113) by immunohisto-chemistry. Their expressions were compared with clinicopathological parameters of tumors. Relationship between maspin and kai1 expression was also concerned in gastric cancer. Results The positive rates of maspin expression were 79.8% (145/182), 75.4% (52/69), and 50.4% (57/113) in normal gastric mucosa, gastric dysplasia, and gastric cancer, while those of kai1 expression were 81.9% (149/182), 65.2% (49/69), and 58.4% (66/113) in corresponding tissues respectively. Gastric cancer less frequently expressed maspin than the normal gastric mucosa and gastric dysplasia (P < 0.05), while dysplasia and cancer showed less frequent expression of kai1 than normal mucosa (P < 0.05). Maspin expression showed negative association with invasive depth, metastasis, Lauren’s and histological classifications (P < 0.05), but not with tumor size, Borrmann’s classification, growth pattern or TNM staging (P > 0.05). Kai1 expression was negatively correlated with invasive depth, metastasis, growth pattern, Lauren’s and histo-logical classifications (P < 0.05), but not with tumor size, Borrmann’s classification or TNM staging (P > 0.05). Maspin and kai1 were collaboratively expressed in gastric cancer (P < 0.05). Conclusions Down-regulated expressions of maspin and kai1 play an important role in gastric carcinogenesis. Abnormal expression of maspin and kai1 might have inhibitory effects on invasion and metastasis of gastric cancer and act as an effe-ctive and objective marker to indicate the pathobiological behaviors of gastric cancer. | Hua-chuanZheng Meng-chunWang Jin-yiLi Xue-feiYang Jin-minSun YanXin | 2004 | Chinese Medical Sciences Journal2004，19，3： | 29 |
| 5 | Variations of mitochondrial D-loop region plus downstream gene 1 2S rRNA-tRNA^(phe) and gastric carcinomas显示文摘AIM: To explore the instabilities, polymorphisms and other variations of mitochondrial D-loop region and downstream gene 12S rRNA-tRNAPhe in gastric cancers, and to study their relationship with gastric cancer.METHODS: Three adjacent regions (D-loop, tRNAphe and 12S rRNA) were detected for instabilities, polymorphisms and other variations via PCR amplification followed by direct DNA sequencing in 22 matched gastric cancerous tissues and para-cancerous normal tissues.RESULTS: PolyC or (CA)n instabilities were detected in 13/22(59.1%) gastric cancers and 9/22(40.9 %) in the control (P>0.05). There existed 2/12(16.7%) and 6/10(60%)alterations of 12S rR NA-tRNAphe in well differentiated gastric cancers and poorly differentiated ones, respectively(P0.05).Some new variations were found, among which np 318 and np 321 C-T transitions in D-loop region were two of the five bases for H-strand replication primer. Np 523 AC-deletion and np 527 C-T transition occurred at mtTF1 binding site (mtTFBS), which were associated with the transcription of downstream mitochondrial genome. Seven samples showed the np 16 182 polyC instabilities, five of which simultaneously showed np 16 189 T-C transitions.CONCLUSION: There is no statistic significance of instabilities and polymorphisms in mitochondrial D-loop region between gastric cancerous and para-cancerous normal tissues, which suggests that the instability might relate to heredity or be dependent on aging. There is asignificant correlation between differentiation degree of gastric cancer and variant frequencies of 12S rRNA-tRNAphe. The poorly differentiated gastric cancers are more prone to 12S rRNAtRNAphe variations, or gastric cancers with 12S rRNA-tRNAphe variations are more likely to be poorly differentiated, np 16189 T-C transition may be one of the important reasons for polyC instability in gastric cancer. | Cheng-BoHan FanLi Yu-JieZhao Jia-MingMa Dong-YingWu Yu-KuiZhang YanXin | 2003 | World Journal of Gastroenterology2003，9，9： | 20 |
| 6 | A Possible Dynamic Mechanism for Rapid Production of the Extreme Hourly Rainfall in Zhengzhou City on 20 July 2021显示文摘In this study,the unprecedented extreme rainfall event during 19-20 July 2021,which caused devastating flooding in Zhengzhou City and its nearby areas,is examined based on observational data analysis and WRF model 40-h simulations on 1-km horizontal resolution.The results show that the model successfully reproduces(i)major synopticscale weather systems(i.e.,the western Pacific subtropical high,the Tibetan high,two typhoons,and the Huang-Huai cyclone),(ii)convective initiation along the east to north edge of the Songshan Mountain,where orographic lifting is obvious,and(iii)subsequent formation of the convective storm producing the extreme rainfall in Zhengzhou.In particular,the model generates the maximum rainfall rate of 233 mm h^(-1)and 40-h accumulated rainfall of 704 mm,corresponding well to the observed extreme values of 201.9 mm h^(-1)and 818 mm,at nearly observed timing and location.Importantly,the model reproduces an intense quasi-stationary,well-organized meso-γ-scale convective system,surrounded by an arc-shaped convergence zone,allowing the development of convective updrafts in a three-quarter circle around the convective system,in a way similar to“multidirectional pumping,”attracting all associated precipitation overlaid and concentrated into the same trailing region to generate the extreme hourly rainfall over Zhengzhou.Our study emphasizes the significant contribution of the unique dynamic structure of the well-organized meso-γ-scale convective system to the record-high hourly rainfall.A possible dynamic mechanism for short-time extreme rainfall production is proposed.That is,the arc-shaped convergence zone of the mesoscale convective system,acting like multidirectional lifting pumps,transports precipitation from different directions into the same region,and thus produces the extreme rainfall.The results gained herein may shed new light on better understanding and forecasting of short-time extreme rainfall. | Jinfang YIN Haodong GU Xudong LIANG Miao YU Jisong SUN Yanxin XIE Feng LI Chong WU | 2022 | Journal of Meteorological Research2022，36，1： | 17 |
| 7 | 胃肠道癌:中国、美国和欧洲显示文摘胃肠道癌包括结直肠癌、胃癌和食管癌,给全球带来了沉重的医疗和经济负担,无论是每年新发病例数还是死亡例数,均居癌症首位。食管癌和胃癌多见于发展中国家,而结直肠癌则是西方国家的主要胃肠道恶性肿瘤。然而,一些经济转型的发展中国家,其胃肠道癌谱正在发生巨大转变,同时,在西方国家,由于某些危险因素的暴露增加,食管癌和胃癌也有抬头的趋势。所有胃肠道癌的发生发展均与生活习惯密切相关,而且都能通过癌前病变予以识别。因此,这些胃肠道癌都非常适宜早期筛查。本文中,我们综述了胃肠道癌在中国、美国、欧洲的流行病学特点,总结其主要危险因素以及这些危险因素在上述地区的分布情况,并对当前的筛查策略进行了探讨。 | Yumo Xie Lishuo Shi Xiaosheng He Yanxin Luo | 2021 | Gastroenterology Report2021，9，2： | 16 |
| 8 | The HuangZaoSi Maize Genome Provides Insights into Genomic Variation and Improvement History of Maize显示文摘Maize is a globally important crop that was a classic model plant for genetic studies. Here, we report a 2.2 Gb draft genome sequence of an elite maize line, HuangZaoSi (HZS). Hybrids bred from HZS-improved lines (HILs) are planted in more than 60% of maize fields in China. Proteome clustering of six completed sequeneed maize genomes show that 638 proteins fall into 264 HZS-specific gene families with the majority of contributions from tandem duplication events. Resequencing and comparative analysis of 40 HZSrelated lines reveals the breeding history of HILs. More than 60% of identified selective sweeps were clustered in identity.by.descent conserved regions, and yield-related genes/QTLs were enriched in HZS characteristic selected regions. Furthermore, we dem on strated that HZS-specific family genes were not uniformly distributed in the genome but enriched in improvement/function.related genomic regions. This study provides an important and novel resource for maize genome research and expands our knowledge on the breadth of genomic variation and improvement history of maize. | Chunhui Li Wei Song Yingfeng Luo Shenghan Gao Ruyang Zhang Zi Shi Xiaqing Wang Ronghuan Wang Fengge Wang Jidong Wang Yanxin Zhao Aiguo Su Shuai Wang Xin Li Meijie Luo Shuaishuai Wang Yunxia Zhang Jianrong Ge Xinyu Tan Ye Yuan Xiaochun Bi Hang He Jianbing Yan Yuandong Wang Songnian Hu Jiuran Zhao | 2019 | Molecular Plant2019，12，3： | 15 |
| 9 | Relationship between abnormality of FHIT gene and EBV infection in gastric cancer显示文摘AIM: To examine the aberrant expression of fragile histidine triad (FHIT) gene and protein in gastric cancer, and to evaluate the role of FHIT gene and the relationship between FHIT gene and EBV infection in gastric carcinogenesis.METHODS: FHIT transcripts were detected by nested RT PCR in 30 cases of gastric cancer and their products were sequenced. FHIT protein was detected by Western blot.EBV infection was detected by PCR method in 50 cases of gastric cancer.RESULTS: The wild type transcripts were detected in all 30 matched normal tissues of gastric cancer. Aberrant transcripts were found in 11/30 (36.7%) gastric cancerous tissues. Sequencing analysis of the aberrant fragments found an RT-PCR product missing exons 5-7 in one case of gastric cancer, and another product missing exons 4-7. Four of ten (40.0%) cases of primary gastric cancer showed absent or decreased expression of FHIT protein as compared with their matched normal tissues. EBV was detected in 5/50 (10%) gastric cancers, among which 4/5 (80%) had aberrant transcripts of FHIT gene. CONCLUSION: Loss of FHIT gene or FHIT protein p1ays an important role in carcinogenesis, development and progression of gastric cancer. EBV infection might influence carcinogenesis of gastric cancer by inducing the abnormality of FHIT gene. | Yu-PingXiao Cheng-BoHan Xiao-YunMao Jin-YiLi LeiXu Chang-ShanRen YanXin | 2005 | World Journal of Gastroenterology2005，11，21： | 15 |
| 10 | TRAIL receptor mediates inflammatory cytokine release in an NF-κB-dependent manner显示文摘在现在的文章,我们报导 DR4 或 DR5 overexpression 戏剧性地在 293T, MDA-MB-231 和 HCT-116 房间以一种 NF-魏 B 依赖者方式激活煽动性的 cytokines IL-8, TNF- 伪, CCL20, MIP-2 和 MIP-1 尾的版本。我们显示出调停受体的信号是的那死亡细胞外域无关,而 DR4 细胞内部的领域的 overexpression 的效果是少得多有势力。表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾,在导致 TNF 的 NF-魏 B 激活起一个必要作用,被发现涉及肿瘤坏死因素相关导致 apoptosis ligand (小道) 调停受体的信号 transduction。表明小径的 FADD-caspase,被报导了主要与 apoptosis 有关,作为与表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾为 DR4 或 DR5 调停 overexpression 的 NF-魏 B 激活和 cytokine 分泌物和串音是必要的被识别。而且, DR5 论战的抗体(AD5-10 ) 触发了煽动性的 cytokine 版本。这些数据和以前的报告,提供小道和小道受体在发炎起一个重要作用的充分证据。 | Wanhu Tang Weimin Wang Yaxi Zhang Shilian Liu Yanxin Liu Dexian Zheng | 2009 | Cell Research2009，19，6： | 14 |
| 11 | Expression of Fas ligand and Caspase-3 contributes to formation of immune escape in gastric cancer显示文摘AIM: To study the role of Fas ligand (FasL) and Caspase-3expression in carcinogenesis and progression of gastric cancer and molecular mechanisms of relevant immune escape.METHODS: FasL and Caspase-3 expression was studied in adjacent epithelial cells, cancer cells and lymphocytes of primary foci, and cancer cells of metastatic foci from 113 cases of gastric cancer by streptavidin-biotin-peroxidase (S-P) immunohistochemistry. Expression of both proteins in cancer cells of primary foci was compared with clinicopathological features of gastric cancer. The relationship between FasL expression in cancer cells and Caspase-3expression in cancer cells or infiltrating lymphocytes of primary foci was investigated.RESULTS: Cancer cells of primary foci expressed FasL in 53.98 % (61/113) of gastric cancers, more than their adjacent epithelial cells (34.51%, 39/113) (P=0.003, X2=8.681), while the expression of Caspase-3 in cancer cells of primary foci was detected in 32.74 % (37/113) of gastric cancers, less than in the adjacent epithelial cells (50.44 %, 57/113)(P=0.007, X2=7.286). Infiltrating lymphocytes of the primary foci showed positive immunoreactivity to Caspase-3 in 70.80 % (80/113) of gastric cancers, more than their corresponding adjacent epithelial cells (P=0.001, X2=10.635)or cancer cells of primary foci (P=0.000, X2=32.767). FasL was less expressed in cancer cells of metastases (51.16 %,22/43) than in those of the corresponding primary foci (81.58 %, 31/38) (P=0.003, X2=9.907). Conversely,Caspase-3 was more expressed in cancer cells of metastases (58.14 %, 25/43) than in those of the corresponding primary foci (34.21%, 13/38) (P=0.031, X2=4.638). FasL expression was significantly correlated with tumor size (P=0.035,rs=0.276), invasive depth (P=0.039, rs=0.195), metastasis (P=0.039, rs=0.195), differentiation (P=0.015, rs=0.228)and Lauren′s classification (P=0.038, rs=0.196), but not with age or gender of patients, growth pattern or TNM staging of gastric cancer (P>0.05). In contrast, Caspase-3 expression showed no correlation with any dinicopathological parameters described above in cancer cells of the primary foci (P>0.05).Interestingly, FasL expression in primary gastric cancer cells paralleled to Caspase-3 expression in infiltrating lymphocytes of the primary foci (P=0.016, X2=5.825).CONCLUSION: Up-regulated expression of FasL and downregulated expression of Caspase-3 in cancer cells of primary foci play an important role in gastric carcinogenesis. As an effective marker to reveal the biological behaviors, FasL is implicated in differentiation, growth, invasion and metastasis of gastric cancer by inducing apoptosis of infiltrating lymphocytes. Chemical substances derived from the primary foci and metastatic microenvironment can inhibit the growth of metastatic cells by enhancing Caspase-3 expression and diminishing FasL expression. | Jin-MinSun Zheng-LiWei Xue-Feiyang Yin-ChangZhang YanXin Hua-ChuanZheng | 2003 | World Journal of Gastroenterology2003，9，7： | 13 |
| 12 | Clean Energy Consumption of Power Systems Towards Smart Agriculture: Roadmap, Bottlenecks and Technologies显示文摘Over the past decades,both agriculture and power systems have faced serious problems,such as the power supply shortage in agriculture,and difficulties of clean energy consump-tion in the power system.To address and overcome these issues,this paper proposes an idea to combine smart agriculture and clean energy consumption,use surplus clean energy to supply agriculture production,and utilize smart agriculture to support power system with clean energy penetration.A comprehensive review has been conducted to first depict the roadmap of coupling a agriculture-clean energy system,analyze their feasibilities and advantages.The recent technologies and bottlenecks are summa-rized and evaluated for the development of a combined system consisting of smart agriculture production and clean energy consumption.Several case studies are introduced to explore the mutual benefits of agriculture-clean energy systems in both the energy and food industries. | Junyong Liu Yanxin Chai Yue Xiang Xin Zhang Si Gou Youbo Liu | 2018 | CSEE Journal of Power and Energy Systems2018，4，3： | 9 |
| 13 | Association Analysis for Quality Traits in a Diverse Panel of Chinese Sesame(Sesamum indicum L.)Germplasm显示文摘这研究的主要目的是评估芝麻(Sesamum indicum L.) 的潜力为协会分析的面板,并且调查油的基因基础内容(OC ) ,蛋白质内容,油的酸集中,和用协会印射的罂酸的酸集中。216 芝麻就职的一块面板是在多环境试用的 phenotyped 并且采指纹, 608 多态的 loci 由 79 份教材生产了,包括简单顺序重复(SSR ) ,顺序相关的放大多型性(SRAP ) ,和放大碎片长度多型性(AFLP ) 。人口结构分析在人口揭示了二亚群。Q 模型为它能力更好表演了重新识别在与另外的三个混合模型相比的高度重要的 P 值的四个特点的协会。并且在 2010 和 2011 的四个特点的 35 和 25 个协会的一个总数被识别分别地,在 Bonferroni 修正以后与 Q 当模特儿。在那些协会之中,仅仅为 OC 的在二环境被重新识别,并且与多重特点同时联系的几个标记被发现。这些结果为它的细微人口层化和家庭关系在这块芝麻面板为营养的特点的协会分析建议 Q 模型的力量和稳定性,它能在把帮助复杂特点,并且能帮助为改进营养的质量开发策略。 | Wenliang Wei Yanxin Zhang Haixia Lü Donghua Li Linhai Wang Xiurong Zhang | 2013 | Journal of Integrative Plant Biology2013，55，8： | 9 |
| 14 | Pathobiological significance of vascular endothelial growth factor and Maspin expressions in human gastric carcinoma显示文摘AIM: To investigate the correlation between expression of vascular endothelial growth factor (VEGF) and cell differentiation, invasion, metastasis and Maspin expression in gastric carcinoma.METHODS: Formalin-fixed paraffin-embedded tissue specimens from 73 cases of gastric carcinoma were studied with SP immunohistochemistry, using anti-VEGF monoclonal antibody, and thirty-nine of them were studied using antiMaspin monoclonal antibody. VEGF expression was compared with the clinical stage, lymph node metastasis, and Borrmann's and WHO's classification of gastric carcinoma.RESULTS: The positive rate of VEGF expression was significantly higher in adjacent non-carcinoma epithelia (ANCE) than in non-metaplastic, non-carcinoma gastric epithelia (NMNCE), which were at least 4 cm distant from the primary tumor (P = 0.000, x^2= 73.03). The positive rate of VEGF expression was significantly higher in advanced gastric carcinoma (AGC) than in early gastric carcinoma (EGC) (P = 0.032, x^2 = 4.62). The positive rate of VEGF expression in gastric carcinomas with lymph node metastases was significantly higher than that in those without metastasis (P = 0.006, x^2 = 7.47). Maspin was weakly expressed in 16 out of 39 cases of NMNCE, and the positive immunoreaction was limited to gland cells of the stomach body. There was no significant correlation between the expression of VEGF and histological or gross classifications, and correlation between the expressions of VEGF and Maspin in gastric carcinoma (P = 0.648, x^2 = 0.21).CONCLUSION: Expression of VEGF is significantly correlated to the malignant biological behaviors of gastric carcinoma,but there is no significant correlation between the expression of VEGF and Maspin. | Jian-JunLi YingChen Su-MinZhang Dong-YingWu Yan-PingWang YanXin | 2004 | World Journal of Gastroenterology2004，10，18： | 7 |
| 15 | Studies of new EST-SSRs derived from Gossypium barbadense显示文摘Existing cotton EST-SSR markers are mostly derived from Gossypium arboreum and Gossypium hir-sutum, but EST-SSR markers from Gossypium barbadense are scarce. One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G. barbadense cv. Pima3-79. Among the SSRs, trinucleotide AAG appeared at a high frequency of 11.76%. 36 accessions (consisting of 13 diploids of the A genome, 11 diploids of the D genome and 12 allotetraploids of the AD genome) were employed to test new EST-SSRs. 76 EST-SSRs were successfully amplified, and 313 polymorphic fragments were yielded, with an average of 4.11 fragments per primer pair. The PIC ranged from 0.17 to 0.95 with an average of 0.53. Based on Jaccard’s genetic similarity coefficient, these 36 accessions were clustered into three groups. 21 EST-SSRs exhibited polymorphisms in BC1 population ((Emian22 × Pima3-79) × Emian22), 24 polymor- phic loci were generated, while 22 of the 24 polymorphic loci were integrated with our interspecific BC1 backbone genetic linkage map, and anchored in 12 chromosomes. This study effectively proved that EST-SSRs from G. barbadense are valuable for genetic diversity analysis and genetic mapping. | ZHANG YanXin LIN ZhongXu LI Wu TU LiLi NIE YiChun ZHANG XianLong | 2007 | Chinese Science Bulletin2007，52，18： | 7 |
| 16 | Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia显示文摘Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality seed and market value. Variation in sesame seed components including oil, oleic acid,linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139 samples collected from different countries. Oil and protein contents were between 40.8%and 60.3%(mean 53.0%) and 15.5% to 25.5%(mean 20.4%), respectively. Linoleic acid,ranging from 31.8% to 52.4%(mean 46%) was more abundant than oleic acid(31.8%–50.6%,mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed West African sesame cultivars containing especially high levels of seed components, which may command high market values. Two clusters of sesame samples grouped by seed composition were obtained, including one cluster with high oil and oleic acid content and the other with high protein and linoleic acid content. This study revealed that sesame samples from Africa and Asia harbor high variation for major seed components and also provided background information for breeding high-nutrition varieties according to the demands of sesame seed markets. | Komivi Dossa Xin Wei Marème Niang Pan Liu Yanxin Zhang Linhai Wang Boshou Liao Ndiaga Cissé Xiurong Zhang Diaga Diouf | 2018 | The Crop Journal2018，6，2： | 7 |
| 17 | On the modification of hypereutectic Al-Si alloys using rare earth Er显示文摘Hypereutectic Al-17.5Si (wt pct) and Al-25Si (wt pct) alloys with various content of rare earth Er were prepared by conventional casting technique. The effect of Er on the microstructure and properties of the hypereutectic Al-Si alloys was investigated using optical microscopy, scanning electron microscopy as well as friction and wear tests. It was found that optimal amount of Er exists in modifying the hypereutectic Al-Si alloys. An appropriate addition of Er improved the anti-wear properties, and reduced the friction coefficient of the hypereutectic Al-Si alloys studied. The primary silicon crystals in the hypereutectic Al-Si alloys were refined with the suitable addition of Er element. The modification mechanism was also discussed in this paper. | Pengfei XING Bo GAO Yanxin ZHUANG Kaihua LIU Ganfeng TU | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，5： | 7 |
| 18 | Microstructure and composition evolution of a single-crystal superalloy caused by elements interdiffusion with an overlay NiCrAlY coating on oxidation显示文摘MCrAlY(M=Ni and/or Co)overlay coating is widely used as a protective coating against high temperature oxidation and corrosion.However,due to its big difference in chemical composition with the underlying superalloy,elements interdiffusion occurs inevitably.One of the direct results is the formation of interdiffusion zone(IDZ)and secondary reaction zone(SRZ)with a high density of fine topological closed-packed phases(TCPs),weakening dramatically the mechanical properties of the alloy substrate.It is by now the main problem of modern high-temperature metallic coatings,but there are still hardly any reports studying the formation,growth and transformation of IDZ and SRZ in deep,as well as the precipitation of TCPs.In this work,a typical NiCrAlY coating is deposited by arc ion plating on a single-crystal superalloy N5.Elements interdiffusion between them and its relationship on microstructure were clarified.Cr rather than Al from the coating diffuses into the alloy at high temperatures and segregates immediately beneath their interface,contributing largely to the formation of IDZ.Simultaneously,diffusion of Ni from the deep alloy to IDZ leads to the formation and continuous expansion of SRZ. | Lanlan Yang Minghui Chen Jinlong Wang Yanxin Qiao Pingyi Guo Shenglong Zhu Fuhui Wang | 2020 | Journal of Materials Science & Technology2020，42，10： | 7 |
| 19 | Surface Water and Groundwater Interactions in Wetlands显示文摘Wetland ecosystems are critical habitats for various types of wild lives and are important components of global ecosystem. However, with climate change and human activities, wetlands are facing with degradation. Surface water and groundwater(SW-GW) interactions play an essential role in matter and energy cycling in wetlands, and therefore affect the evolution and health of wetlands. But the role of groundwater in wetland ecosystems has been neglected or simplified. In this paper, we reviewed how surface water interacts with groundwater, and made a systematic summarization of the role of SW-GW interactions(such as maintaining water balance and biological diversity and removing pollution) in wetland ecological functions. We also reviewed the methods to investigate, simulate and quantify SW-GW interactions and related reactions. Finally, we illustrated how climate change and human activities affect SW-GW interactions and therefore affect wetland functions. We highlight the importance of groundwater in wetlands and the urgency to intensify the research in integrated multidisciplinary monitoring and simulation methods, dominant variables and thresholds and integrated water resources management of SW-GW interactions, and further aim to stimulate better protection and restoration of wetlands all over the world. | Xiancang Wu Teng Ma Yanxin Wang | 2020 | Journal of Earth Science2020，31，5： | 6 |
| 20 | The effect of PET calculations in DRAINMOD on drainage and crop yields predictions in a subhumid vertisol soil district显示文摘The field hydrology model-DRAINMOD has several options for evapotranspiration (ET) calculation. The choice of each calculation method may affect modeling results because ET is one of the most important hydrologic components in field hydrology. ET calculation method may also have significant impact on crop yield predictions. As variable ET estimation methods are available nowadays, this paper presents a study of different ET inputs on simulated crop yields and drainage volume based on weather and soil data from a subhumid climate zone in China. The study results show that the effect of ET estimate on drainage and crop yield prediction is affected by crop species and growing period; more accurate ET estimate is also critical in simulating the effect of controlled drainage on field hydrology and crop production. The results of this study indicate that the effort contributed to better ET estimate is very important in model applications. | LUO Wan JING WeiHua JIA ZhongHua LI Jin PAN YanXin | 2009 | Science China(Technological Sciences)2009，52，11： | 6 |
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