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| 1 | Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability显示文摘The unicellular cyanobacterium, Synechococcus elongatus PCC 7942(Syn7942), synthesizes sucrose as the only compatible solute under salt stress. A series of engineered Syn7942 strains for sucrose production were constructed. The overexpression of the native sps(encoding a natively fused protein of sucrose phosphate synthase SPS and sucrose phosphate phosphatase SPP) in Syn7942 wild type caused a 93% improvement of sucrose productivity. The strain FL130 co-overexpressing sps and csc B(encoding a sucrose transporter) exhibited a 74% higher extracellular sucrose production than that overexpressing csc B only. Both results showed the significant improvement of sucrose productivity by the double functional protein SPS-SPP. Afterwards, FL130 was cultivated under a modified condition, and the cell-free culture medium containing 1.5 g L-1 sucrose was pre-treated with an acid hydrolysis technique. Cultivated with the neutralized hydrolysates as the starting media, two widely used microorganisms, Escherichia coli and Saccharomyces cerevisiae, showed a comparable growth with that in the control media supplemented with glucose. These results clearly demonstrated that the cell-free culture of sucrose-secreting cyanobacteria can be applied as starting media in microbial cultivation. | DUAN Yangkai LUO Quan LIANG Feiyan LU Xuefeng | 2016 | Journal of Ocean University of China2016，15，5： | 3 |
| 2 | Switching mechanism and optimisation research on a pressure-attitude adaptive adjusting coal seam water jet slotter显示文摘To investigate the attitude-switching mechanisms of existing jet slotters,which integrate drilling,punching and slotting operations,and to improve its fracture ability,we used the power bond diagram theory to analyse the dynamic flow pressure,and force of slotters.A mathematical model was developed for the dynamic characteristics of slotter systems.Furthermore,to study the effect of the main characteristic parameters on the ability of the nozzle to erode sandstone,multi-orthogonal experiments were carried out.And the optimised slots were applied in later practical operations.The research results show that the inlet fluid passed through the time-varying orifice to generate pressure differential thrust,which overcame the spring force,pushed the valve core to open the side nozzle,and closed the rear cavity channel thereby realising the switch of the slotter attitude.An optimal plan was established to balance the diameter,depth,and volume of punching,and a rock-breaking plan was developed for the slotter.Subsequently,the optimised water jet slotter was practically used in coal seam gas drainage.Compared with conventional dense drilling,water jet slotting technology significantly improves the ability,efficiency,and effect of increasing the permeability of the coal seam. | Yiyu Lu Yangkai Zhang Jiren Tang Qi Yao | 2022 | International Journal of Mining Science and Technology2022，32，6： | 1 |
| 3 | Exploring the photosynthetic production capacity of sucrose by cyanobacteria显示文摘 | Wei Du Feiyan Liang Yangkai Duan Xiaoming Tan Xuefeng Lu | 2013 | Metabolic Engineering2013，，： | 1 |
| 4 | Aldosterone dysregulation predicts the risk of mortality and rehospitalization in heart failure with a preserved ejection fraction显示文摘Serum aldosterone is associated with cardiac remodeling,which contributes to morbidity and mortality in heart failure(HF);however,the prognostic value of aldosterone in HF with a preserved ejection fraction(HFpEF)is unclear.We used liquid chromatography-tandem mass spectrometry to quantify serum aldosterone in 873 patients with HFpEF in a Registry Study of Biomarkers for HF.The retrospective study was conducted at Beijing Anzhen Hospital from May 2017 to October 2019.The primary endpoint was a composite of all-cause mortality and rehospitalization for HF.Aldosterone concentrations in patients with and without events were 124.22 pmol L^(-1)(interquartile range(IQR):48.62–256.20)and 96.33 pmol L^(-1)(IQR:37.33–215.76),respectively(P=0.023).Aldosterone independently predicted all-cause mortality(adjusted hazard ratio(aHR),1.55;95%confidence interval(95%CI),1.06–2.27;P=0.024)and the primary endpoint(a HR,1.43;95%CI,1.11–1.85;P=0.006).Patients with high aldosterone concentrations were at higher risk of concentric remodeling(adjusted odds ratio(aOR),1.45;95%CI,1.03–2.04;P=0.034).Patients with high aldosterone and B-type natriuretic peptide concentrations were at a higher risk of the primary endpoint(hazard ratio(HR),1.85;95%CI,1.29–2.66;P=0.001).We conclude that elevated aldosterone is associated with a risk of rehospitalization with HF and all-cause mortality in patients with HFpEF. | Bingbing Ke Xin Tan Lu Ren Yangkai Fan Yixin Zhang Fengjuan Li Qiqi Sun Tong Liu Lixin Jia Yuan Wang Jie Du | 2022 | Science China(Life Sciences)2022，65，3： | 1 |
| 5 | Construction of Industrialization Development Model for'1+4+X'Community-Based Elderly Care in Gannan Old Revolutionary Base Area in the Context of Population Aging显示文摘In the context of controlling population development in a planned way,China entered an aging society in the early 21 st century.In this context,how to meet the needs of the elderly care and form an effective elderly care model has become a key problem to be solved urgently by local government departments.With the continuous advancement of the elderly care policy,the community-based elderly care has gradually become the mainstream and is in the stage of vigorous promotion.Taking the Gannan old revolutionary base area in Jiangxi Province as an example,this study puts forward'1+4+X'community-based elderly care model based on the policy system of community-based elderly care,the physical and mental health of the elderly,the material space and the construction of evaluation system,and explores how to promote the application of this community model efficiently in the form of industrialization,so as to drive the economic growth of the Gannan old revolutionary base area,promote the employment development and improve the community-based elderly care service. | Chenxin YANG Jun XIAO Liyuan WANG Jiaxin LIU Yangkai LU | 2023 | Asian Agricultural Research2023，15，5： | 0 |
| 6 | Formation Principle and Characteristics of Self‑Supercharging Pulsed Water Jet显示文摘High-pressure pulsed water jet technology has considerable development potential in the field of rock fragmentation.To overcome the shortcomings of existing pulsed jets,a self-supercharging pulsed water jet(SSPWJ)generation method is proposed,which is based on the theory of the pulsed water jet and the principle of hydraulic boosting.The proposed method changes the flow direction of the fluid medium through the valve core to make the piston reciprocate in the cylinder and relies on the effective area difference between the front and rear chambers in the stroke stage of the piston to realize the organic combination of“pulse”and“supercharging”of the jet,thus forming an SSPWJ.On the basis of the formation principle of the SSPWJ,a SSPWJ testing platform was constructed,and tests were performed on the jet pressure acquisition,morphology capture,and granite erosion.Both the jet pressure and the jet morphology exhibited periodic changes,and a higher pulse pressure was obtained at lower inlet pressure.The error of the pressure ratio calculated according to the experimental results was<3%relative to the theoretical design value,confirming the feasibility of the method.The pulse pressure and pulse frequency are controllable;that is,as the inlet flow rate increases in the stroke stage of the piston,the pulse pressure and pulse frequency increase,and the pulse duration decreases.As the inlet flow rate increases in the backward-stroke stage of the piston,the pulse frequency increases,and the pulse pressure and pulse duration remain unchanged.Under the combined action of the waterhammer pressure,high-speed lateral flow,and high-frequency dynamic load of the SSPWJ,local flaky exfoliation was observed when the granite surface was eroded.The results of this study lay the foundation for enriching the theory of pulsed jet generation and expanding its application range. | Zhaolong Ge Yuanfei Ling Jiren Tang Yiyu Lu Yangkai Zhang Lei Wang Qi Yao | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
| 7 | A New Mobile Sliding Door for Rural Elderly-friendly Suspended Bathroom and Toilet in the Context of Rural Population Aging显示文摘Taking the rural left-behind elderly as the research object,this paper made an in-depth analysis of the living needs of the rural'empty-nest elderly'.Combined with the current market development situation of the elderly-friendly bathroom sliding door in China,it proposed the design scheme and industrial development strategy of the suspended bathroom sliding door,to provide a certain reference for the elderly-friendly development of home decoration basic materials for elderly homes in rural areas. | Jianjian HE Yufeng WU Yangkai LU Zengyan TU Yihan JIAO | 2022 | Asian Agricultural Research2022，14，6： | 0 |
| 8 | Improving anti-Gram-positive-bacterial performance and osteogenesis for zinc alloy via mussel mimetic polydopamine with non-antibiotic lysozyme and parathyroid hormone显示文摘In this work,a novel coating with a non-antibiotic agent for inhibiting Gram-positive bacteria and promoting osteogenesis was prepared on zinc-aluminium alloy(ZA6-1)via mussel mimetic polydopamine(PDA)containing lysozyme(LYS)and parathyroid hormone(PTH).The results indicate that as-deposited coatings can efficiently decrease the degradation rate of ZA6-1 from 0.52 to 0.16 mm/year,and the addition of LYS weakens the coating resistance,while the addition of PTH enhances the coating resis-tance.In spite that no obvious inhibition of Escherichia coli is observed,the coated zinc alloys show good in vitro antibacterial performance against Staphylococcus aureus.Compared with ZA6-1 zinc alloys,the increase of antibacterial efficacy reaches 86.9%-90.1%.Furthermore,the lower hydrophilicity(26.4°),higher osteoblast cell viability(>100%),good osteoblast cell morphology and better osteoblast cell differentiation(ALP=107.7%)for PDA-LYS/PTH coated samples support that as-prepared coating is promising for modifying biodegradable zinc implants. | Deren Zhao Tianhao Chen Jinqian Chen Banglun Huang Jihua Nie Hulin Liu Yangkai Liu Zilin Chen Feng Zou Qiqi Lu Xiaodong Tan Yunjie Xiang Jing Li Yongping Zhang Xue Feng Xi Rao | 2023 | Biosurface and Biotribology2023，9，4： | 0 |
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