|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability显示文摘The unicellular cyanobacterium, Synechococcus elongatus PCC 7942(Syn7942), synthesizes sucrose as the only compatible solute under salt stress. A series of engineered Syn7942 strains for sucrose production were constructed. The overexpression of the native sps(encoding a natively fused protein of sucrose phosphate synthase SPS and sucrose phosphate phosphatase SPP) in Syn7942 wild type caused a 93% improvement of sucrose productivity. The strain FL130 co-overexpressing sps and csc B(encoding a sucrose transporter) exhibited a 74% higher extracellular sucrose production than that overexpressing csc B only. Both results showed the significant improvement of sucrose productivity by the double functional protein SPS-SPP. Afterwards, FL130 was cultivated under a modified condition, and the cell-free culture medium containing 1.5 g L-1 sucrose was pre-treated with an acid hydrolysis technique. Cultivated with the neutralized hydrolysates as the starting media, two widely used microorganisms, Escherichia coli and Saccharomyces cerevisiae, showed a comparable growth with that in the control media supplemented with glucose. These results clearly demonstrated that the cell-free culture of sucrose-secreting cyanobacteria can be applied as starting media in microbial cultivation. | DUAN Yangkai LUO Quan LIANG Feiyan LU Xuefeng | 2016 | Journal of Ocean University of China2016，15，5： | 3 |
| 2 | Tomographic SAR imaging with large elevation aperture: a P-band small UAV demonstration显示文摘Elevation resolution is an important indicator in tomographic SAR imaging as it represents the ability to discriminate closed targets in elevation. In general, the elevation resolution is proportional to the length of the elevation aperture. However, as the elevation aperture increases, the geometric consistency of the image will undesirably deteriorate and hence fails the image coregistration approach required by the traditional super-resolution tomographic imaging. In this paper, a new super-resolution tomographic imaging method is proposed to overcome the inconsistency problem caused by the large elevation aperture. The core strategy is to get rid of two-dimensional image coregistration by applying a three-dimensional(3 D) back projection like imaging manner: the 3 D space is firstly divided into a 3 D imaging grid, each of which is individually imaged via compressive sensing for super-resolution. The effectiveness of the proposed approach is evaluated by both computer simulations and real P-band UAV SAR data. | Tao ZENG Minkun LIU Yan WANG Zegang DING Linghao LI Zhen WANG Yangkai WEI Jianping WANG | 2022 | Science China(Information Sciences)2022，65，3： | 2 |
| 3 | Malicious Code Modeling and Analysis in Weighted Scale-Free Networks显示文摘We study the detailed malicious code propagating process in scale-free networks with link weights that denotes traffic between two nodes. It is found that the propagating velocity reaches a peak rapidly then decays in a power-law form, which is different from the well-known result in unweighted network case. Simulation results show that the nodes with larger strength are preferential to be infected, but the hierarchical dynamics are not clearly found. The simulation results also show that larger disper- sion of weight of networks leads to slower propagating, which indicates that malicious code propagates more quickly in unweighted scale-free net- works than in weighted scale-free networks under the same condition. These results show that not only the topology of networks but also the link weights affect the malicious propagating process. | WANG Changguang WANG Fangwei ZHANG Yangkai MA Jianfengi | 2007 | Wuhan University Journal of Natural Sciences2007，12，1： | 2 |
| 4 | Investigating thermal residual stress effect on mechanical behaviors of fiber composites with different fiber arrays 显示文摘 | Tsai JiaLin Chi YangKai | 2008 | Composites: PartB2008，39，4： | 1 |
| 5 | Switching mechanism and optimisation research on a pressure-attitude adaptive adjusting coal seam water jet slotter显示文摘To investigate the attitude-switching mechanisms of existing jet slotters,which integrate drilling,punching and slotting operations,and to improve its fracture ability,we used the power bond diagram theory to analyse the dynamic flow pressure,and force of slotters.A mathematical model was developed for the dynamic characteristics of slotter systems.Furthermore,to study the effect of the main characteristic parameters on the ability of the nozzle to erode sandstone,multi-orthogonal experiments were carried out.And the optimised slots were applied in later practical operations.The research results show that the inlet fluid passed through the time-varying orifice to generate pressure differential thrust,which overcame the spring force,pushed the valve core to open the side nozzle,and closed the rear cavity channel thereby realising the switch of the slotter attitude.An optimal plan was established to balance the diameter,depth,and volume of punching,and a rock-breaking plan was developed for the slotter.Subsequently,the optimised water jet slotter was practically used in coal seam gas drainage.Compared with conventional dense drilling,water jet slotting technology significantly improves the ability,efficiency,and effect of increasing the permeability of the coal seam. | Yiyu Lu Yangkai Zhang Jiren Tang Qi Yao | 2022 | International Journal of Mining Science and Technology2022，32，6： | 1 |
| 6 | Exploring the photosynthetic production capacity of sucrose by cyanobacteria显示文摘 | Wei Du Feiyan Liang Yangkai Duan Xiaoming Tan Xuefeng Lu | 2013 | Metabolic Engineering2013，，： | 1 |
| 7 | 显示文摘 | SHURu-xin(束茹欣) SUNPing(孙平) YANGKai(杨凯) ZHANGJian-ping(张建平) LIUTai-ang(刘太昂) | 2011 | 烟草科技2011，292，： | 1 |
| 8 | Self-generating nanogaps for highly effective surface-enhanced Raman spectroscopy显示文摘The fabrication of surface enhanced Raman spectroscopy(SERS)substrates with controlled high density hot spots still remains challenging.Herein,we report highly effective SERS substrates containing the self-generating(SG)nanogaps from polystyrene nanosphere monolayer through isotropic plasma etching.The emergence of multimode hot spots,i.e.,metal film over nanosphere(MFON)-like hot spots(closed gaps,0 nm),individual self-aligned hot spots(discrete gaps,>20 nm)and threedimensional(3D)hot spots(nanogaps,1-10 nm),makes the SG SERS substrates superior as compared to the traditional MFON or the well-ordered self-aligned SERS substrates in terms of enhancement,uniformity,and reproducibility.The SG SERS substrates can function as the excellent SERS platforms for trace molecule detection in the practical application fields. | Yangkai Chen Huan Li Jianmei Chen Dong Li Mengyuan Zhang Guanghua Yu Lin Jiang Yi Zong Bin Dong Zhoufang Zeng Yandong Wang Lifeng Chi | 2022 | Nano Research2022，15，4： | 1 |
| 9 | Titanium carbide nanosheets with defect structure for photothermal-enhanced sonodynamic therapy显示文摘Sonodynamic therapy(SDT)has attracted widespread interest in biomedicine,owing to its novel and noninvasive therapeutic method triggered by ultrasound(US).Herein,the Ti_(3)C_(2) MXene nanosheets(Ti_(3)C_(2) NSs)are developed as good sonosensitizers via a two-step method of chemical exfoliation and high-temperature treatment.With the high-temperature treatment,the oxygen defect of Ti_(3)C_(2) MXene nanosheets(H-Ti_(3)C_(2) NSs)is greatly increased.Therefore,the electron(e^(-))and hole(h^(+))generated by US can be separated faster due to the improved degree of oxidation,and then the recombination of e^(-)-h^(+)can be prevented with the abundant oxygen defect under US irradiation,which induced the sonodynamic efficiency greatly to improve around 3.7-fold compared with Ti_(3)C_(2) NSs without high-temperature treatment.After PEGylation,the H-Ti_(3)C_(2)-PEG NSs show good stability and biocompatibility.In vitro studies exhibit that the inherent property of mild photothermal effect can promote the endocytosis of H-Ti_(3)C_(2)-PEG NSs,which can improve the SDT efficacy.In vivo studies further display that the increased blood supply by the mild photothermal effect can significantly relieve hypoxia in the tumor microenvironment,showing photothermal therapy(PTT)enhanced SDT.Most importantly,the H-Ti_(3)C_(2)-PEG NSs can be biodegraded and excreted out of the body,showing no significant long-term toxicity.Our work develops the defective H-Ti_(3)C_(2) NSs as high-efficiency and safe sonosensitizers for photothermal-enhanced SDT of cancer,extending the biomedical application of MXene-based nanoplatforms. | Guangqiang Li Xiaoyan Zhong Xianwen Wang Fei Gong Huali Lei Yangkai Zhou Chengfei Li Zhidong Xiao Guoxi Ren Liang Zhang Zhiqiang Dong Zhuang Liu Liang Cheng | 2022 | Bioactive Materials2022，7，2： | 1 |
| 10 | Aldosterone dysregulation predicts the risk of mortality and rehospitalization in heart failure with a preserved ejection fraction显示文摘Serum aldosterone is associated with cardiac remodeling,which contributes to morbidity and mortality in heart failure(HF);however,the prognostic value of aldosterone in HF with a preserved ejection fraction(HFpEF)is unclear.We used liquid chromatography-tandem mass spectrometry to quantify serum aldosterone in 873 patients with HFpEF in a Registry Study of Biomarkers for HF.The retrospective study was conducted at Beijing Anzhen Hospital from May 2017 to October 2019.The primary endpoint was a composite of all-cause mortality and rehospitalization for HF.Aldosterone concentrations in patients with and without events were 124.22 pmol L^(-1)(interquartile range(IQR):48.62–256.20)and 96.33 pmol L^(-1)(IQR:37.33–215.76),respectively(P=0.023).Aldosterone independently predicted all-cause mortality(adjusted hazard ratio(aHR),1.55;95%confidence interval(95%CI),1.06–2.27;P=0.024)and the primary endpoint(a HR,1.43;95%CI,1.11–1.85;P=0.006).Patients with high aldosterone concentrations were at higher risk of concentric remodeling(adjusted odds ratio(aOR),1.45;95%CI,1.03–2.04;P=0.034).Patients with high aldosterone and B-type natriuretic peptide concentrations were at a higher risk of the primary endpoint(hazard ratio(HR),1.85;95%CI,1.29–2.66;P=0.001).We conclude that elevated aldosterone is associated with a risk of rehospitalization with HF and all-cause mortality in patients with HFpEF. | Bingbing Ke Xin Tan Lu Ren Yangkai Fan Yixin Zhang Fengjuan Li Qiqi Sun Tong Liu Lixin Jia Yuan Wang Jie Du | 2022 | Science China(Life Sciences)2022，65，3： | 1 |
| 11 | The mechanism and therapy of aortic aneurysms显示文摘Aortic aneurysm is a chronic aortic disease affected by many factors.Although it is generally asymptomatic,it poses a significant threat to human life due to a high risk of rupture.Because of its strong concealment,it is difficult to diagnose the disease in the early stage.At present,there are no effective drugs for the treatment of aneurysms.Surgical intervention and endovascular treatment are the only therapies.Although current studies have discovered that inflammatory responses as well as the production and activation of various proteases promote aortic aneurysm,the specific mechanisms remain unclear.Researchers are further exploring the pathogenesis of aneurysms to find new targets for diagnosis and treatment.To better understand aortic aneurysm,this review elaborates on the discovery history of aortic aneurysm,main classification and clinical manifestations,related molecular mechanisms,clinical cohort studies and animal models,with the ultimate goal of providing insights into the treatment of this devastating disease.The underlying problem with aneurysm disease is weakening of the aortic wall,leading to progressive dilation.If not treated in time,the aortic aneurysm eventually ruptures.An aortic aneurysm is a local enlargement of an artery caused by a weakening of the aortic wall.The disease is usually asymptomatic but leads to high mortality due to the risk of artery rupture. | Jianing Gao Huanhuan Cao Gaofei Hu Yufei Wu Yangkai Xu Hongtu Cui Hong S.Lu Lemin Zheng | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 1 |
| 12 | 显示文摘 | YANGKai CAIJia-yue ZHANGChao-ping etal(杨凯 蔡嘉月 张朝平 等) | 2014 | 光谱学与光谱分析2014，34，12： | 1 |
| 13 | 显示文摘 | RENYong-Guo(任永国) LIUZi-Qiang(刘自强) YANGKai(杨凯) etal | | ZhongguoTaoci0，，： | 1 |
| 14 | 显示文摘 | YUChang-Lin(余长林) YANGKai(杨凯) YUJi-Mei(余济美) etal | | WuliHuaxueXuebao0，，： | 1 |
| 15 | Price Discovery Function of Agricultural Futures Market in China--Based on VECM-PT-IS and DCC-MGARCH-t models显示文摘Agricultural futures market plays an important role in financial system,and its function of price discovery and hedging is of great significance to the long-term price stability for agricultural products.However,in China,agricultural futures market is still in construction stage,and scholars have not fully studied its price discovery function.Hence,this study will investigate the price discovery function of China agricultural futures market.The causal relationship,price contribution degree and volatility spillover effect of futures and spot markets are studied by comparing the price discovery function of soybean,yellow corn and soybean oil futures and spot.Taking the average daily settlement price of futures and spot in Dalian Commodity Exchange as study objects,the VECM and PT-IS model is used to investigate the causal relationship and the difference in price contribution between them.Then DDC-MGARCH-t model is used to analyze their volatility spillover effect.The empirical results show that there is obvious mutual guiding relationship between agricultural futures and spot market,and the price contribution of futures is significantly higher than that of spot,proving that agricultural futures have the function of price discovery.Meanwhile,the volatility spillover effect between agricultural futures and spot is bidirectional.The impact of internal fluctuations is often greater than that of external shocks. | Yangkai Guo | 2018 | 经济管理学刊(中英文版)2018，7，2： | 1 |
| 16 | Construction of Industrialization Development Model for'1+4+X'Community-Based Elderly Care in Gannan Old Revolutionary Base Area in the Context of Population Aging显示文摘In the context of controlling population development in a planned way,China entered an aging society in the early 21 st century.In this context,how to meet the needs of the elderly care and form an effective elderly care model has become a key problem to be solved urgently by local government departments.With the continuous advancement of the elderly care policy,the community-based elderly care has gradually become the mainstream and is in the stage of vigorous promotion.Taking the Gannan old revolutionary base area in Jiangxi Province as an example,this study puts forward'1+4+X'community-based elderly care model based on the policy system of community-based elderly care,the physical and mental health of the elderly,the material space and the construction of evaluation system,and explores how to promote the application of this community model efficiently in the form of industrialization,so as to drive the economic growth of the Gannan old revolutionary base area,promote the employment development and improve the community-based elderly care service. | Chenxin YANG Jun XIAO Liyuan WANG Jiaxin LIU Yangkai LU | 2023 | Asian Agricultural Research2023，15，5： | 0 |
| 17 | Effects of two vectors on the expression of the NbNAC1 transcription factor and preparation of its polyclonal antibody显示文摘The NAC(NAM,ATAF,and CUC)superfamily is one of the largest plant-specific families containing transcription factors.An increasing number of studies suggest that NAC1 is involved in plants response to different biotic and abiotic stimulis.Nicotiana benthamiana is a widely used system for evaluating plant-pathogen interactions.In order to study the biochemical function of NbNAC1,NbNAC1 protein and antibody are essential.Therefore,we focused on developing a prokaryotic expression system for producing the Nicotiana benthamiana NbNAC1 protein of in Escherichia coli and the preparation of its polyclonal antibody.Firstly,we constructed two different molecular weight prokaryotic expression vectors:pGE vector with GST tag(pGEX4T-1–NbNAC1)and pET expression vector with His tag(pET28a-NbNAC1).The NbNAC1 protein can be successfully expressed in both vectors.The His-tagged NbNAC1 proteins are insoluble,while the GST-tagged NbNAC1 proteins are partially soluble.We then successfully purified and enriched both proteins.The His-tagged NbNAC1 was chosen to immunize rabbits owing to an unknown protein accompanying the GST-tagged NbNAC1.The anti-NbNAC1 polyclonal antibody had good specificity and could be used in subsequent protein-related studies. | FENG ZHU QINQIN ZHANG YANGKAI ZHOU QIPING ZHANG MENGYAO CAO ZHAOLIN JI | 2022 | BIOCELL2022，46，9： | 0 |
| 18 | Formation Principle and Characteristics of Self‑Supercharging Pulsed Water Jet显示文摘High-pressure pulsed water jet technology has considerable development potential in the field of rock fragmentation.To overcome the shortcomings of existing pulsed jets,a self-supercharging pulsed water jet(SSPWJ)generation method is proposed,which is based on the theory of the pulsed water jet and the principle of hydraulic boosting.The proposed method changes the flow direction of the fluid medium through the valve core to make the piston reciprocate in the cylinder and relies on the effective area difference between the front and rear chambers in the stroke stage of the piston to realize the organic combination of“pulse”and“supercharging”of the jet,thus forming an SSPWJ.On the basis of the formation principle of the SSPWJ,a SSPWJ testing platform was constructed,and tests were performed on the jet pressure acquisition,morphology capture,and granite erosion.Both the jet pressure and the jet morphology exhibited periodic changes,and a higher pulse pressure was obtained at lower inlet pressure.The error of the pressure ratio calculated according to the experimental results was<3%relative to the theoretical design value,confirming the feasibility of the method.The pulse pressure and pulse frequency are controllable;that is,as the inlet flow rate increases in the stroke stage of the piston,the pulse pressure and pulse frequency increase,and the pulse duration decreases.As the inlet flow rate increases in the backward-stroke stage of the piston,the pulse frequency increases,and the pulse pressure and pulse duration remain unchanged.Under the combined action of the waterhammer pressure,high-speed lateral flow,and high-frequency dynamic load of the SSPWJ,local flaky exfoliation was observed when the granite surface was eroded.The results of this study lay the foundation for enriching the theory of pulsed jet generation and expanding its application range. | Zhaolong Ge Yuanfei Ling Jiren Tang Yiyu Lu Yangkai Zhang Lei Wang Qi Yao | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 0 |
| 19 | A New Mobile Sliding Door for Rural Elderly-friendly Suspended Bathroom and Toilet in the Context of Rural Population Aging显示文摘Taking the rural left-behind elderly as the research object,this paper made an in-depth analysis of the living needs of the rural'empty-nest elderly'.Combined with the current market development situation of the elderly-friendly bathroom sliding door in China,it proposed the design scheme and industrial development strategy of the suspended bathroom sliding door,to provide a certain reference for the elderly-friendly development of home decoration basic materials for elderly homes in rural areas. | Jianjian HE Yufeng WU Yangkai LU Zengyan TU Yihan JIAO | 2022 | Asian Agricultural Research2022，14，6： | 0 |
| 20 | Improving anti-Gram-positive-bacterial performance and osteogenesis for zinc alloy via mussel mimetic polydopamine with non-antibiotic lysozyme and parathyroid hormone显示文摘In this work,a novel coating with a non-antibiotic agent for inhibiting Gram-positive bacteria and promoting osteogenesis was prepared on zinc-aluminium alloy(ZA6-1)via mussel mimetic polydopamine(PDA)containing lysozyme(LYS)and parathyroid hormone(PTH).The results indicate that as-deposited coatings can efficiently decrease the degradation rate of ZA6-1 from 0.52 to 0.16 mm/year,and the addition of LYS weakens the coating resistance,while the addition of PTH enhances the coating resis-tance.In spite that no obvious inhibition of Escherichia coli is observed,the coated zinc alloys show good in vitro antibacterial performance against Staphylococcus aureus.Compared with ZA6-1 zinc alloys,the increase of antibacterial efficacy reaches 86.9%-90.1%.Furthermore,the lower hydrophilicity(26.4°),higher osteoblast cell viability(>100%),good osteoblast cell morphology and better osteoblast cell differentiation(ALP=107.7%)for PDA-LYS/PTH coated samples support that as-prepared coating is promising for modifying biodegradable zinc implants. | Deren Zhao Tianhao Chen Jinqian Chen Banglun Huang Jihua Nie Hulin Liu Yangkai Liu Zilin Chen Feng Zou Qiqi Lu Xiaodong Tan Yunjie Xiang Jing Li Yongping Zhang Xue Feng Xi Rao | 2023 | Biosurface and Biotribology2023，9，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员